Oceanisphaera avium
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2484 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0CXV7|A0A1Y0CXV7_9GAMM Glycerol-3-phosphate acyltransferase OS=Oceanisphaera avium OX=1903694 GN=plsY PE=3 SV=1
MM1 pKa = 7.67 NIGLFYY7 pKa = 11.06 GSTTCYY13 pKa = 10.67 TEE15 pKa = 3.74 MAAEE19 pKa = 4.63 KK20 pKa = 10.19 IGEE23 pKa = 4.01 QLGAEE28 pKa = 4.38 LVDD31 pKa = 3.28 LHH33 pKa = 6.62 NIKK36 pKa = 10.53 DD37 pKa = 3.78 VPLARR42 pKa = 11.84 AQDD45 pKa = 3.92 YY46 pKa = 8.97 PILILGISTWDD57 pKa = 3.44 FGEE60 pKa = 4.3 LQEE63 pKa = 5.19 DD64 pKa = 4.56 WEE66 pKa = 4.73 SHH68 pKa = 5.62 WDD70 pKa = 5.36 DD71 pKa = 4.88 IDD73 pKa = 3.97 QLDD76 pKa = 4.2 LNGHH80 pKa = 4.84 VVALFGMGDD89 pKa = 3.32 QLGYY93 pKa = 11.02 GEE95 pKa = 4.72 WFQDD99 pKa = 4.14 ALGLLHH105 pKa = 7.38 DD106 pKa = 4.43 KK107 pKa = 10.96 VIAQGATVVGYY118 pKa = 8.69 WPNVGYY124 pKa = 10.49 EE125 pKa = 4.16 FEE127 pKa = 4.19 ASKK130 pKa = 11.24 ALTADD135 pKa = 3.28 GEE137 pKa = 4.85 YY138 pKa = 10.63 FVGLSLDD145 pKa = 3.57 EE146 pKa = 4.73 ANQYY150 pKa = 11.02 DD151 pKa = 4.36 DD152 pKa = 3.55 SDD154 pKa = 3.92 ARR156 pKa = 11.84 IQSWVAQILEE166 pKa = 4.19 EE167 pKa = 4.12 MAVLL171 pKa = 4.31
Molecular weight: 19.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.554
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.884
Patrickios 0.744
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|A0A1Y0D069|A0A1Y0D069_9GAMM Uncharacterized protein OS=Oceanisphaera avium OX=1903694 GN=CBP12_13175 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.2 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 KK22 pKa = 7.89 ATVGGRR28 pKa = 11.84 KK29 pKa = 9.05 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.61 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2484
0
2484
797316
28
2604
321.0
35.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.142 ± 0.055
0.996 ± 0.017
5.103 ± 0.037
5.889 ± 0.046
3.755 ± 0.034
6.998 ± 0.05
2.509 ± 0.026
5.636 ± 0.032
4.297 ± 0.035
11.811 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.495 ± 0.024
3.6 ± 0.035
4.293 ± 0.03
5.262 ± 0.051
5.226 ± 0.035
6.098 ± 0.037
5.022 ± 0.029
6.794 ± 0.042
1.332 ± 0.025
2.743 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here