Ezakiella coagulans
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1626 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U1E2J3|A0A2U1E2J3_9FIRM Branched-chain amino acid aminotransferase OS=Ezakiella coagulans OX=46507 GN=C7381_10854 PE=4 SV=1
MM1 pKa = 8.02 DD2 pKa = 3.54 EE3 pKa = 4.68 HH4 pKa = 6.93 KK5 pKa = 10.56 KK6 pKa = 9.39 HH7 pKa = 6.82 HH8 pKa = 6.78 NDD10 pKa = 4.04 DD11 pKa = 3.77 EE12 pKa = 4.79 HH13 pKa = 7.06 EE14 pKa = 4.44 CTCGCGCGCHH24 pKa = 6.98 DD25 pKa = 4.34 EE26 pKa = 5.46 DD27 pKa = 5.86 EE28 pKa = 4.39 MVEE31 pKa = 4.01 YY32 pKa = 8.9 DD33 pKa = 3.62 TVNITFEE40 pKa = 4.35 DD41 pKa = 4.05 DD42 pKa = 3.91 KK43 pKa = 11.53 EE44 pKa = 4.51 SEE46 pKa = 4.47 CAVLDD51 pKa = 3.52 QFKK54 pKa = 10.45 IGEE57 pKa = 4.23 KK58 pKa = 10.12 EE59 pKa = 4.16 YY60 pKa = 10.56 MVLLPLEE67 pKa = 4.82 DD68 pKa = 3.91 NEE70 pKa = 4.41 EE71 pKa = 4.07 EE72 pKa = 3.77 LVYY75 pKa = 10.19 IYY77 pKa = 10.43 RR78 pKa = 11.84 YY79 pKa = 8.4 TDD81 pKa = 3.47 DD82 pKa = 3.9 EE83 pKa = 4.66 NGSFTLDD90 pKa = 3.54 VIEE93 pKa = 6.36 DD94 pKa = 3.81 EE95 pKa = 5.33 DD96 pKa = 4.64 EE97 pKa = 4.59 LDD99 pKa = 3.78 EE100 pKa = 4.62 VCDD103 pKa = 3.79 KK104 pKa = 11.3 FEE106 pKa = 4.34 EE107 pKa = 4.25 RR108 pKa = 11.84 AEE110 pKa = 4.14 LNEE113 pKa = 4.13 EE114 pKa = 4.07
Molecular weight: 13.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.63
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.961
Wikipedia 3.668
Rodwell 3.643
Grimsley 3.541
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.05
Thurlkill 3.656
EMBOSS 3.694
Sillero 3.935
Patrickios 0.668
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|A0A2U1E5J1|A0A2U1E5J1_9FIRM Phosphoglycerol transferase MdoB-like AlkP superfamily enzyme OS=Ezakiella coagulans OX=46507 GN=C7381_102105 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 9.68 QPKK8 pKa = 8.57 NRR10 pKa = 11.84 QRR12 pKa = 11.84 KK13 pKa = 7.76 KK14 pKa = 7.74 VHH16 pKa = 5.67 GFRR19 pKa = 11.84 EE20 pKa = 3.96 RR21 pKa = 11.84 MRR23 pKa = 11.84 TKK25 pKa = 10.51 NGRR28 pKa = 11.84 NVLKK32 pKa = 10.48 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 NRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.85 VLSAA44 pKa = 4.11
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.906
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.069
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1626
0
1626
533091
27
4123
327.9
37.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.801 ± 0.063
0.811 ± 0.027
6.426 ± 0.054
7.88 ± 0.061
4.802 ± 0.045
6.421 ± 0.057
1.358 ± 0.024
8.8 ± 0.078
9.093 ± 0.066
8.839 ± 0.073
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.752 ± 0.038
5.645 ± 0.058
2.996 ± 0.048
2.075 ± 0.032
3.916 ± 0.045
5.983 ± 0.053
5.092 ± 0.064
6.62 ± 0.053
0.606 ± 0.018
4.084 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here