Vibrio phage Phriendly

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9N9B1|A0A5B9N9B1_9CAUD Uncharacterized protein OS=Vibrio phage Phriendly OX=2596675 GN=CPT_Phriendly_044 PE=4 SV=1
MM1 pKa = 7.76AIIKK5 pKa = 9.85VITHH9 pKa = 6.48KK10 pKa = 10.71LGNITEE16 pKa = 5.21DD17 pKa = 2.83IHH19 pKa = 8.63VDD21 pKa = 3.3IAAGSCACPICGDD34 pKa = 3.98YY35 pKa = 11.23VDD37 pKa = 5.81LLIDD41 pKa = 3.67EE42 pKa = 5.32DD43 pKa = 4.43GDD45 pKa = 4.04YY46 pKa = 9.63KK47 pKa = 8.53TTCDD51 pKa = 4.83CDD53 pKa = 3.54IDD55 pKa = 5.44DD56 pKa = 4.19YY57 pKa = 11.86VEE59 pKa = 4.44KK60 pKa = 10.89GDD62 pKa = 3.85YY63 pKa = 10.95NDD65 pKa = 4.1MEE67 pKa = 4.71EE68 pKa = 4.53EE69 pKa = 4.27LDD71 pKa = 5.11DD72 pKa = 5.55LRR74 pKa = 11.84DD75 pKa = 4.13DD76 pKa = 3.55YY77 pKa = 12.13GEE79 pKa = 4.09AVKK82 pKa = 10.63EE83 pKa = 3.72RR84 pKa = 11.84DD85 pKa = 3.62NFEE88 pKa = 5.49LEE90 pKa = 3.96VDD92 pKa = 3.84DD93 pKa = 6.9LKK95 pKa = 11.59LQLEE99 pKa = 4.3NLKK102 pKa = 10.74ASYY105 pKa = 9.94EE106 pKa = 4.02ADD108 pKa = 2.99IEE110 pKa = 4.16ALEE113 pKa = 4.46EE114 pKa = 4.16EE115 pKa = 4.81YY116 pKa = 10.45QQKK119 pKa = 8.19ITVRR123 pKa = 11.84DD124 pKa = 4.17GIIDD128 pKa = 3.93DD129 pKa = 5.54LEE131 pKa = 4.2EE132 pKa = 3.72QLAATRR138 pKa = 11.84EE139 pKa = 4.2EE140 pKa = 4.24

Molecular weight: 15.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N8I5|A0A5B9N8I5_9CAUD Uncharacterized protein OS=Vibrio phage Phriendly OX=2596675 GN=CPT_Phriendly_013 PE=4 SV=1
MM1 pKa = 7.57ACKK4 pKa = 9.52QVKK7 pKa = 9.89LNRR10 pKa = 11.84KK11 pKa = 7.18MAAKK15 pKa = 10.12ASRR18 pKa = 11.84MTKK21 pKa = 10.17GNEE24 pKa = 4.12SVTGNTDD31 pKa = 3.63RR32 pKa = 11.84IMRR35 pKa = 11.84DD36 pKa = 3.07AEE38 pKa = 4.26NLFHH42 pKa = 7.07RR43 pKa = 11.84SKK45 pKa = 11.01RR46 pKa = 11.84GTIYY50 pKa = 10.69SASCKK55 pKa = 9.59GARR58 pKa = 3.89

Molecular weight: 6.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

15442

34

919

217.5

24.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.959 ± 0.391

1.179 ± 0.149

6.605 ± 0.201

7.169 ± 0.411

3.685 ± 0.206

7.486 ± 0.374

2.111 ± 0.173

5.569 ± 0.211

7.026 ± 0.37

7.68 ± 0.295

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.044 ± 0.133

5.563 ± 0.22

3.549 ± 0.204

3.983 ± 0.256

4.527 ± 0.189

5.479 ± 0.3

5.589 ± 0.244

6.495 ± 0.186

1.574 ± 0.152

3.73 ± 0.149
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski