Amino acid dipepetide frequency for Vibrio phage Marilyn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.789AlaAla: 9.789 ± 1.189
0.455AlaCys: 0.455 ± 0.188
5.919AlaAsp: 5.919 ± 0.681
6.754AlaGlu: 6.754 ± 0.695
3.111AlaPhe: 3.111 ± 0.433
6.298AlaGly: 6.298 ± 0.741
1.442AlaHis: 1.442 ± 0.352
5.236AlaIle: 5.236 ± 0.822
6.754AlaLys: 6.754 ± 0.593
6.83AlaLeu: 6.83 ± 0.729
1.821AlaMet: 1.821 ± 0.425
3.718AlaAsn: 3.718 ± 0.4
3.035AlaPro: 3.035 ± 0.407
4.25AlaGln: 4.25 ± 0.473
4.022AlaArg: 4.022 ± 0.523
5.008AlaSer: 5.008 ± 0.564
5.767AlaThr: 5.767 ± 0.492
5.995AlaVal: 5.995 ± 0.692
1.214AlaTrp: 1.214 ± 0.29
2.428AlaTyr: 2.428 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
0.531CysAla: 0.531 ± 0.17
0.076CysCys: 0.076 ± 0.071
0.986CysAsp: 0.986 ± 0.31
0.607CysGlu: 0.607 ± 0.222
0.379CysPhe: 0.379 ± 0.265
0.759CysGly: 0.759 ± 0.228
0.304CysHis: 0.304 ± 0.124
0.455CysIle: 0.455 ± 0.174
0.455CysLys: 0.455 ± 0.187
0.228CysLeu: 0.228 ± 0.131
0.304CysMet: 0.304 ± 0.172
0.152CysAsn: 0.152 ± 0.085
0.379CysPro: 0.379 ± 0.174
0.379CysGln: 0.379 ± 0.205
0.759CysArg: 0.759 ± 0.247
0.304CysSer: 0.304 ± 0.17
0.531CysThr: 0.531 ± 0.163
0.835CysVal: 0.835 ± 0.262
0.076CysTrp: 0.076 ± 0.084
0.152CysTyr: 0.152 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
5.16AspAla: 5.16 ± 0.76
0.683AspCys: 0.683 ± 0.241
4.25AspAsp: 4.25 ± 0.611
5.767AspGlu: 5.767 ± 0.518
2.884AspPhe: 2.884 ± 0.41
5.236AspGly: 5.236 ± 0.486
0.835AspHis: 0.835 ± 0.339
3.567AspIle: 3.567 ± 0.432
3.263AspLys: 3.263 ± 0.457
4.25AspLeu: 4.25 ± 0.579
1.138AspMet: 1.138 ± 0.267
2.504AspAsn: 2.504 ± 0.317
1.897AspPro: 1.897 ± 0.423
2.732AspGln: 2.732 ± 0.505
2.125AspArg: 2.125 ± 0.374
4.932AspSer: 4.932 ± 0.597
3.187AspThr: 3.187 ± 0.393
4.857AspVal: 4.857 ± 0.557
0.531AspTrp: 0.531 ± 0.148
2.58AspTyr: 2.58 ± 0.437
0.0AspXaa: 0.0 ± 0.0
Glu
6.374GluAla: 6.374 ± 0.665
0.531GluCys: 0.531 ± 0.161
3.415GluAsp: 3.415 ± 0.505
5.388GluGlu: 5.388 ± 1.041
2.201GluPhe: 2.201 ± 0.38
3.642GluGly: 3.642 ± 0.46
1.821GluHis: 1.821 ± 0.316
5.236GluIle: 5.236 ± 0.623
5.008GluLys: 5.008 ± 0.726
7.816GluLeu: 7.816 ± 1.055
2.125GluMet: 2.125 ± 0.511
4.022GluAsn: 4.022 ± 0.536
2.808GluPro: 2.808 ± 0.503
3.263GluGln: 3.263 ± 0.545
3.946GluArg: 3.946 ± 0.531
4.629GluSer: 4.629 ± 0.634
3.567GluThr: 3.567 ± 0.397
4.477GluVal: 4.477 ± 0.711
1.366GluTrp: 1.366 ± 0.302
2.352GluTyr: 2.352 ± 0.399
0.0GluXaa: 0.0 ± 0.0
Phe
3.187PheAla: 3.187 ± 0.493
0.379PheCys: 0.379 ± 0.156
3.718PheAsp: 3.718 ± 0.461
1.821PheGlu: 1.821 ± 0.332
1.366PhePhe: 1.366 ± 0.302
2.808PheGly: 2.808 ± 0.387
0.607PheHis: 0.607 ± 0.207
1.594PheIle: 1.594 ± 0.278
2.58PheLys: 2.58 ± 0.464
1.594PheLeu: 1.594 ± 0.411
0.911PheMet: 0.911 ± 0.322
2.884PheAsn: 2.884 ± 0.453
1.138PhePro: 1.138 ± 0.221
1.29PheGln: 1.29 ± 0.245
1.29PheArg: 1.29 ± 0.291
2.049PheSer: 2.049 ± 0.4
2.808PheThr: 2.808 ± 0.442
2.049PheVal: 2.049 ± 0.33
0.455PheTrp: 0.455 ± 0.192
1.973PheTyr: 1.973 ± 0.411
0.0PheXaa: 0.0 ± 0.0
Gly
5.919GlyAla: 5.919 ± 0.758
0.759GlyCys: 0.759 ± 0.197
3.718GlyAsp: 3.718 ± 0.591
4.477GlyGlu: 4.477 ± 0.62
2.808GlyPhe: 2.808 ± 0.391
6.83GlyGly: 6.83 ± 1.03
1.062GlyHis: 1.062 ± 0.269
3.567GlyIle: 3.567 ± 0.456
5.388GlyLys: 5.388 ± 0.514
4.401GlyLeu: 4.401 ± 0.517
1.669GlyMet: 1.669 ± 0.325
4.25GlyAsn: 4.25 ± 0.436
1.366GlyPro: 1.366 ± 0.312
2.201GlyGln: 2.201 ± 0.383
3.111GlyArg: 3.111 ± 0.428
4.401GlySer: 4.401 ± 0.624
3.567GlyThr: 3.567 ± 0.457
5.084GlyVal: 5.084 ± 0.52
1.518GlyTrp: 1.518 ± 0.297
2.352GlyTyr: 2.352 ± 0.553
0.0GlyXaa: 0.0 ± 0.0
His
1.138HisAla: 1.138 ± 0.32
0.076HisCys: 0.076 ± 0.075
1.138HisAsp: 1.138 ± 0.313
0.911HisGlu: 0.911 ± 0.299
1.138HisPhe: 1.138 ± 0.299
1.214HisGly: 1.214 ± 0.275
0.607HisHis: 0.607 ± 0.247
1.062HisIle: 1.062 ± 0.292
1.138HisLys: 1.138 ± 0.382
1.442HisLeu: 1.442 ± 0.348
0.455HisMet: 0.455 ± 0.175
0.759HisAsn: 0.759 ± 0.196
1.062HisPro: 1.062 ± 0.296
0.531HisGln: 0.531 ± 0.188
1.29HisArg: 1.29 ± 0.314
1.29HisSer: 1.29 ± 0.306
1.29HisThr: 1.29 ± 0.284
1.29HisVal: 1.29 ± 0.354
0.228HisTrp: 0.228 ± 0.148
0.683HisTyr: 0.683 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
4.174IleAla: 4.174 ± 0.548
0.304IleCys: 0.304 ± 0.15
5.464IleAsp: 5.464 ± 0.774
5.008IleGlu: 5.008 ± 0.704
1.669IlePhe: 1.669 ± 0.279
4.629IleGly: 4.629 ± 0.568
1.138IleHis: 1.138 ± 0.265
2.352IleIle: 2.352 ± 0.395
4.401IleLys: 4.401 ± 0.589
3.567IleLeu: 3.567 ± 0.454
1.062IleMet: 1.062 ± 0.274
3.718IleAsn: 3.718 ± 0.515
1.594IlePro: 1.594 ± 0.368
2.277IleGln: 2.277 ± 0.402
2.58IleArg: 2.58 ± 0.543
4.781IleSer: 4.781 ± 0.79
4.629IleThr: 4.629 ± 0.774
3.415IleVal: 3.415 ± 0.578
0.759IleTrp: 0.759 ± 0.235
1.594IleTyr: 1.594 ± 0.332
0.0IleXaa: 0.0 ± 0.0
Lys
6.374LysAla: 6.374 ± 0.661
0.455LysCys: 0.455 ± 0.181
3.491LysAsp: 3.491 ± 0.653
4.629LysGlu: 4.629 ± 0.592
1.594LysPhe: 1.594 ± 0.33
3.642LysGly: 3.642 ± 0.476
1.594LysHis: 1.594 ± 0.386
4.477LysIle: 4.477 ± 0.647
5.615LysLys: 5.615 ± 0.687
6.602LysLeu: 6.602 ± 0.691
1.973LysMet: 1.973 ± 0.335
3.111LysAsn: 3.111 ± 0.31
2.732LysPro: 2.732 ± 0.495
3.339LysGln: 3.339 ± 0.387
2.884LysArg: 2.884 ± 0.443
3.946LysSer: 3.946 ± 0.594
4.174LysThr: 4.174 ± 0.442
3.718LysVal: 3.718 ± 0.413
0.986LysTrp: 0.986 ± 0.31
2.732LysTyr: 2.732 ± 0.381
0.0LysXaa: 0.0 ± 0.0
Leu
7.057LeuAla: 7.057 ± 0.845
1.214LeuCys: 1.214 ± 0.363
3.946LeuAsp: 3.946 ± 0.568
5.767LeuGlu: 5.767 ± 0.687
2.959LeuPhe: 2.959 ± 0.505
4.401LeuGly: 4.401 ± 0.63
1.366LeuHis: 1.366 ± 0.327
4.098LeuIle: 4.098 ± 0.665
5.691LeuLys: 5.691 ± 0.779
4.25LeuLeu: 4.25 ± 0.669
1.594LeuMet: 1.594 ± 0.293
4.629LeuAsn: 4.629 ± 0.612
3.718LeuPro: 3.718 ± 0.505
2.352LeuGln: 2.352 ± 0.37
4.477LeuArg: 4.477 ± 0.759
6.45LeuSer: 6.45 ± 0.644
4.174LeuThr: 4.174 ± 0.509
4.553LeuVal: 4.553 ± 0.55
0.835LeuTrp: 0.835 ± 0.233
1.897LeuTyr: 1.897 ± 0.324
0.0LeuXaa: 0.0 ± 0.0
Met
2.884MetAla: 2.884 ± 0.482
0.076MetCys: 0.076 ± 0.081
1.29MetAsp: 1.29 ± 0.291
1.745MetGlu: 1.745 ± 0.373
1.062MetPhe: 1.062 ± 0.318
1.518MetGly: 1.518 ± 0.405
0.379MetHis: 0.379 ± 0.182
1.442MetIle: 1.442 ± 0.356
1.594MetLys: 1.594 ± 0.246
1.973MetLeu: 1.973 ± 0.359
0.911MetMet: 0.911 ± 0.254
0.911MetAsn: 0.911 ± 0.186
0.911MetPro: 0.911 ± 0.251
1.366MetGln: 1.366 ± 0.244
1.897MetArg: 1.897 ± 0.434
1.214MetSer: 1.214 ± 0.254
1.897MetThr: 1.897 ± 0.388
1.518MetVal: 1.518 ± 0.334
0.076MetTrp: 0.076 ± 0.067
1.138MetTyr: 1.138 ± 0.319
0.0MetXaa: 0.0 ± 0.0
Asn
6.222AsnAla: 6.222 ± 0.602
0.379AsnCys: 0.379 ± 0.178
2.428AsnAsp: 2.428 ± 0.405
3.339AsnGlu: 3.339 ± 0.606
1.821AsnPhe: 1.821 ± 0.359
3.946AsnGly: 3.946 ± 0.595
0.986AsnHis: 0.986 ± 0.286
2.049AsnIle: 2.049 ± 0.357
2.959AsnLys: 2.959 ± 0.417
4.857AsnLeu: 4.857 ± 0.649
1.214AsnMet: 1.214 ± 0.285
2.959AsnAsn: 2.959 ± 0.432
2.504AsnPro: 2.504 ± 0.453
1.973AsnGln: 1.973 ± 0.352
3.263AsnArg: 3.263 ± 0.451
3.187AsnSer: 3.187 ± 0.506
2.049AsnThr: 2.049 ± 0.344
2.201AsnVal: 2.201 ± 0.411
0.607AsnTrp: 0.607 ± 0.199
2.504AsnTyr: 2.504 ± 0.396
0.0AsnXaa: 0.0 ± 0.0
Pro
3.035ProAla: 3.035 ± 0.469
0.228ProCys: 0.228 ± 0.172
3.111ProAsp: 3.111 ± 0.641
3.035ProGlu: 3.035 ± 0.499
1.366ProPhe: 1.366 ± 0.372
1.973ProGly: 1.973 ± 0.352
1.062ProHis: 1.062 ± 0.352
2.428ProIle: 2.428 ± 0.427
2.049ProLys: 2.049 ± 0.35
2.808ProLeu: 2.808 ± 0.488
1.062ProMet: 1.062 ± 0.253
1.29ProAsn: 1.29 ± 0.331
1.745ProPro: 1.745 ± 0.487
1.366ProGln: 1.366 ± 0.284
1.366ProArg: 1.366 ± 0.472
2.808ProSer: 2.808 ± 0.469
2.656ProThr: 2.656 ± 0.435
2.201ProVal: 2.201 ± 0.45
0.379ProTrp: 0.379 ± 0.166
1.062ProTyr: 1.062 ± 0.276
0.0ProXaa: 0.0 ± 0.0
Gln
2.732GlnAla: 2.732 ± 0.569
0.379GlnCys: 0.379 ± 0.189
2.125GlnAsp: 2.125 ± 0.25
2.58GlnGlu: 2.58 ± 0.414
1.366GlnPhe: 1.366 ± 0.241
2.201GlnGly: 2.201 ± 0.316
0.683GlnHis: 0.683 ± 0.219
2.959GlnIle: 2.959 ± 0.42
2.049GlnLys: 2.049 ± 0.348
4.098GlnLeu: 4.098 ± 0.61
1.138GlnMet: 1.138 ± 0.251
2.504GlnAsn: 2.504 ± 0.395
1.897GlnPro: 1.897 ± 0.357
1.518GlnGln: 1.518 ± 0.284
2.656GlnArg: 2.656 ± 0.468
2.201GlnSer: 2.201 ± 0.371
2.428GlnThr: 2.428 ± 0.312
2.277GlnVal: 2.277 ± 0.441
0.911GlnTrp: 0.911 ± 0.257
1.214GlnTyr: 1.214 ± 0.246
0.0GlnXaa: 0.0 ± 0.0
Arg
4.022ArgAla: 4.022 ± 0.569
0.455ArgCys: 0.455 ± 0.183
2.504ArgAsp: 2.504 ± 0.644
3.87ArgGlu: 3.87 ± 0.614
2.201ArgPhe: 2.201 ± 0.419
2.428ArgGly: 2.428 ± 0.424
1.138ArgHis: 1.138 ± 0.257
4.022ArgIle: 4.022 ± 0.637
3.035ArgLys: 3.035 ± 0.542
4.022ArgLeu: 4.022 ± 0.561
1.518ArgMet: 1.518 ± 0.324
1.973ArgAsn: 1.973 ± 0.415
1.973ArgPro: 1.973 ± 0.446
2.352ArgGln: 2.352 ± 0.41
1.897ArgArg: 1.897 ± 0.386
2.884ArgSer: 2.884 ± 0.614
3.187ArgThr: 3.187 ± 0.513
2.58ArgVal: 2.58 ± 0.395
0.759ArgTrp: 0.759 ± 0.23
1.138ArgTyr: 1.138 ± 0.327
0.0ArgXaa: 0.0 ± 0.0
Ser
5.995SerAla: 5.995 ± 0.82
0.607SerCys: 0.607 ± 0.196
4.098SerAsp: 4.098 ± 0.53
6.222SerGlu: 6.222 ± 0.645
2.884SerPhe: 2.884 ± 0.5
4.477SerGly: 4.477 ± 0.672
1.214SerHis: 1.214 ± 0.313
4.932SerIle: 4.932 ± 0.819
5.464SerLys: 5.464 ± 0.639
3.491SerLeu: 3.491 ± 0.455
2.277SerMet: 2.277 ± 0.403
3.263SerAsn: 3.263 ± 0.52
2.049SerPro: 2.049 ± 0.393
1.897SerGln: 1.897 ± 0.386
1.821SerArg: 1.821 ± 0.355
3.642SerSer: 3.642 ± 0.517
3.718SerThr: 3.718 ± 0.607
4.857SerVal: 4.857 ± 0.59
0.911SerTrp: 0.911 ± 0.27
1.897SerTyr: 1.897 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
5.919ThrAla: 5.919 ± 0.715
0.455ThrCys: 0.455 ± 0.159
4.401ThrAsp: 4.401 ± 0.484
3.415ThrGlu: 3.415 ± 0.522
1.973ThrPhe: 1.973 ± 0.341
4.932ThrGly: 4.932 ± 0.491
1.29ThrHis: 1.29 ± 0.323
3.794ThrIle: 3.794 ± 0.545
3.642ThrLys: 3.642 ± 0.633
6.222ThrLeu: 6.222 ± 0.724
1.062ThrMet: 1.062 ± 0.292
2.277ThrAsn: 2.277 ± 0.44
2.352ThrPro: 2.352 ± 0.381
2.277ThrGln: 2.277 ± 0.343
2.049ThrArg: 2.049 ± 0.39
4.098ThrSer: 4.098 ± 0.451
3.491ThrThr: 3.491 ± 0.562
3.946ThrVal: 3.946 ± 0.444
0.607ThrTrp: 0.607 ± 0.193
2.277ThrTyr: 2.277 ± 0.387
0.0ThrXaa: 0.0 ± 0.0
Val
5.767ValAla: 5.767 ± 0.546
0.531ValCys: 0.531 ± 0.159
3.263ValAsp: 3.263 ± 0.388
4.401ValGlu: 4.401 ± 0.702
1.973ValPhe: 1.973 ± 0.368
3.946ValGly: 3.946 ± 0.448
0.835ValHis: 0.835 ± 0.261
3.415ValIle: 3.415 ± 0.496
3.794ValLys: 3.794 ± 0.661
3.718ValLeu: 3.718 ± 0.613
2.201ValMet: 2.201 ± 0.484
4.325ValAsn: 4.325 ± 0.984
1.821ValPro: 1.821 ± 0.412
2.125ValGln: 2.125 ± 0.494
4.098ValArg: 4.098 ± 0.45
4.401ValSer: 4.401 ± 0.656
4.325ValThr: 4.325 ± 0.471
4.174ValVal: 4.174 ± 0.451
1.138ValTrp: 1.138 ± 0.258
2.277ValTyr: 2.277 ± 0.312
0.0ValXaa: 0.0 ± 0.0
Trp
0.911TrpAla: 0.911 ± 0.238
0.304TrpCys: 0.304 ± 0.178
0.911TrpAsp: 0.911 ± 0.231
1.214TrpGlu: 1.214 ± 0.327
0.683TrpPhe: 0.683 ± 0.221
0.986TrpGly: 0.986 ± 0.254
0.304TrpHis: 0.304 ± 0.137
0.911TrpIle: 0.911 ± 0.21
0.759TrpLys: 0.759 ± 0.231
1.442TrpLeu: 1.442 ± 0.378
0.379TrpMet: 0.379 ± 0.159
0.835TrpAsn: 0.835 ± 0.228
0.152TrpPro: 0.152 ± 0.103
0.455TrpGln: 0.455 ± 0.25
0.531TrpArg: 0.531 ± 0.164
0.911TrpSer: 0.911 ± 0.252
0.835TrpThr: 0.835 ± 0.255
0.531TrpVal: 0.531 ± 0.165
0.379TrpTrp: 0.379 ± 0.158
0.607TrpTyr: 0.607 ± 0.22
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.732TyrAla: 2.732 ± 0.434
0.304TyrCys: 0.304 ± 0.125
2.201TyrAsp: 2.201 ± 0.388
3.035TyrGlu: 3.035 ± 0.396
1.062TyrPhe: 1.062 ± 0.255
2.58TyrGly: 2.58 ± 0.447
0.076TyrHis: 0.076 ± 0.065
1.518TyrIle: 1.518 ± 0.347
2.428TyrLys: 2.428 ± 0.443
1.821TyrLeu: 1.821 ± 0.361
0.911TyrMet: 0.911 ± 0.26
1.366TyrAsn: 1.366 ± 0.277
1.897TyrPro: 1.897 ± 0.439
1.973TyrGln: 1.973 ± 0.355
1.897TyrArg: 1.897 ± 0.42
2.58TyrSer: 2.58 ± 0.424
2.201TyrThr: 2.201 ± 0.434
1.973TyrVal: 1.973 ± 0.338
0.304TyrTrp: 0.304 ± 0.131
1.062TyrTyr: 1.062 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (13179 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski