Mycobacterium virus Dorothy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7KDR3|J7KDR3_9CAUD DNA methylase OS=Mycobacterium virus Dorothy OX=1225861 GN=75 PE=3 SV=1
MM1 pKa = 7.38NATEE5 pKa = 5.72DD6 pKa = 3.71GLEE9 pKa = 4.22PLGEE13 pKa = 4.45APDD16 pKa = 4.23LGAPRR21 pKa = 11.84YY22 pKa = 9.14PMSAEE27 pKa = 4.13VTFHH31 pKa = 7.62RR32 pKa = 11.84ARR34 pKa = 11.84CTKK37 pKa = 10.5CGDD40 pKa = 3.08IEE42 pKa = 4.31TDD44 pKa = 3.37YY45 pKa = 11.76GDD47 pKa = 4.22FSAYY51 pKa = 10.11SDD53 pKa = 3.39PGGAINAVLAAADD66 pKa = 3.49WFGRR70 pKa = 11.84SAPTGEE76 pKa = 5.08LIDD79 pKa = 4.08FGGGRR84 pKa = 11.84MGQRR88 pKa = 11.84YY89 pKa = 7.81QLVEE93 pKa = 4.45LLCPDD98 pKa = 3.6CRR100 pKa = 11.84RR101 pKa = 11.84CEE103 pKa = 3.99VCGTAKK109 pKa = 10.44AYY111 pKa = 9.85PIDD114 pKa = 3.85DD115 pKa = 4.0HH116 pKa = 6.93LVCEE120 pKa = 4.29DD121 pKa = 4.12HH122 pKa = 7.29EE123 pKa = 4.31DD124 pKa = 3.94HH125 pKa = 6.79EE126 pKa = 5.25FEE128 pKa = 4.96AAPP131 pKa = 3.95

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7KM93|J7KM93_9CAUD Minor tail protein OS=Mycobacterium virus Dorothy OX=1225861 GN=17 PE=4 SV=1
MM1 pKa = 7.17QLRR4 pKa = 11.84IIRR7 pKa = 11.84GGAMSSDD14 pKa = 4.14FWWGMFVIPAAALAVAGVLVAVMAVIWASAKK45 pKa = 9.67WGGNEE50 pKa = 3.63YY51 pKa = 10.91KK52 pKa = 10.34LWPKK56 pKa = 10.29RR57 pKa = 11.84WGQRR61 pKa = 11.84EE62 pKa = 4.31SIVTVVATAKK72 pKa = 9.94SVRR75 pKa = 11.84YY76 pKa = 8.89LWIPGWHH83 pKa = 5.78IVICRR88 pKa = 11.84TTMATQEE95 pKa = 4.67SRR97 pKa = 11.84PEE99 pKa = 3.5WQRR102 pKa = 11.84RR103 pKa = 11.84LRR105 pKa = 11.84VQHH108 pKa = 6.91AIGAAIRR115 pKa = 11.84AEE117 pKa = 4.23EE118 pKa = 4.07EE119 pKa = 3.92AA120 pKa = 4.55

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

18761

30

1168

180.4

19.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.33 ± 0.425

1.269 ± 0.157

6.641 ± 0.235

5.986 ± 0.329

2.969 ± 0.185

8.992 ± 0.613

2.212 ± 0.191

4.349 ± 0.187

3.438 ± 0.213

7.372 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.13

3.385 ± 0.173

5.938 ± 0.213

3.545 ± 0.173

7.036 ± 0.411

5.73 ± 0.297

6.386 ± 0.28

7.137 ± 0.269

2.484 ± 0.177

2.548 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski