BtNv-AlphaCoV/SC2013

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Nyctacovirus; Nyctalus velutinus alphacoronavirus SC-2013

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1WHF2|A0A0U1WHF2_9ALPC Membrane protein OS=BtNv-AlphaCoV/SC2013 OX=1503291 GN=M PE=3 SV=1
MM1 pKa = 7.78KK2 pKa = 10.1INLGLPPSTSSFVSGYY18 pKa = 10.85LPTPGNWTCRR28 pKa = 11.84NGGTTKK34 pKa = 10.76LQGHH38 pKa = 6.65ARR40 pKa = 11.84AVFMRR45 pKa = 11.84YY46 pKa = 8.29YY47 pKa = 10.57AYY49 pKa = 10.61ARR51 pKa = 11.84AWSFGVGPSTTIDD64 pKa = 3.22GHH66 pKa = 6.16YY67 pKa = 10.63GLYY70 pKa = 9.68VWHH73 pKa = 6.99SNSAGHH79 pKa = 4.81MTVRR83 pKa = 11.84ICRR86 pKa = 11.84WYY88 pKa = 10.36QDD90 pKa = 3.88RR91 pKa = 11.84DD92 pKa = 3.98PIAQNSPHH100 pKa = 6.99DD101 pKa = 4.18TTSSDD106 pKa = 3.6CILNVRR112 pKa = 11.84QPYY115 pKa = 9.54VFTHH119 pKa = 6.91AGFQVIGVSWTGEE132 pKa = 3.68AVTVYY137 pKa = 10.27GKK139 pKa = 10.63SKK141 pKa = 10.55LYY143 pKa = 10.24RR144 pKa = 11.84LYY146 pKa = 11.28VPGASLWNSVAFTCSKK162 pKa = 10.9DD163 pKa = 3.62DD164 pKa = 3.61SCGHH168 pKa = 5.98QIITKK173 pKa = 9.35PITVNATTDD182 pKa = 3.47DD183 pKa = 3.7KK184 pKa = 11.83GVITSYY190 pKa = 8.59TVCDD194 pKa = 3.49RR195 pKa = 11.84CDD197 pKa = 3.45GFPHH201 pKa = 6.72HH202 pKa = 6.86VFAVQEE208 pKa = 4.26GGKK211 pKa = 9.69IPGSFDD217 pKa = 3.13FTNWFYY223 pKa = 10.92LTNTSSPFDD232 pKa = 3.35GRR234 pKa = 11.84FVSNQPLQIQCLWPIPALTSTTGIIYY260 pKa = 9.97FNSSRR265 pKa = 11.84FTDD268 pKa = 3.11PHH270 pKa = 5.65QRR272 pKa = 11.84CNGYY276 pKa = 10.2NEE278 pKa = 4.11VGGLADD284 pKa = 3.87HH285 pKa = 6.86LRR287 pKa = 11.84FAINVTDD294 pKa = 4.3RR295 pKa = 11.84GAFQLGVISLLAVHH309 pKa = 6.45NAYY312 pKa = 10.43NFSCSNISNFVDD324 pKa = 3.11ATTIGVPFGKK334 pKa = 8.9TYY336 pKa = 10.21QPYY339 pKa = 8.43YY340 pKa = 10.61CFVTEE345 pKa = 5.37GYY347 pKa = 10.21NISANRR353 pKa = 11.84TFVGVMPSDD362 pKa = 3.33VRR364 pKa = 11.84EE365 pKa = 4.22IVVSRR370 pKa = 11.84YY371 pKa = 9.41GSVYY375 pKa = 9.83INGYY379 pKa = 10.08KK380 pKa = 9.84IFNVGEE386 pKa = 4.09LYY388 pKa = 10.95GVVLNFSSLTGSDD401 pKa = 3.63FWTVAYY407 pKa = 10.32ANEE410 pKa = 4.18VNVLVDD416 pKa = 3.32IEE418 pKa = 4.33EE419 pKa = 4.44TYY421 pKa = 9.3ITGILYY427 pKa = 10.44CDD429 pKa = 3.55TPLNRR434 pKa = 11.84LKK436 pKa = 10.75CQQQRR441 pKa = 11.84FFMDD445 pKa = 3.65DD446 pKa = 3.2GFYY449 pKa = 10.89SAIDD453 pKa = 3.5LAPPVVQTIVLLPEE467 pKa = 3.89YY468 pKa = 10.05TGLTNITLDD477 pKa = 3.5VNVTFNPPSCIQCAPTVNSILLNGEE502 pKa = 4.08SGGSVCVSTNRR513 pKa = 11.84FSVDD517 pKa = 3.51FKK519 pKa = 11.11LTVNSQAYY527 pKa = 10.03DD528 pKa = 2.96IGVRR532 pKa = 11.84TGSCPFSYY540 pKa = 10.85GSLNNFVKK548 pKa = 10.71FGSICFSLVDD558 pKa = 3.98NGGCPMPINAINYY571 pKa = 8.97IKK573 pKa = 10.0IEE575 pKa = 4.01YY576 pKa = 8.66PIGVLYY582 pKa = 8.93VTHH585 pKa = 6.75SPGEE589 pKa = 4.41AITGVPKK596 pKa = 10.97GFIKK600 pKa = 10.83RR601 pKa = 11.84LGFLDD606 pKa = 4.89ASILHH611 pKa = 6.46LNVCTEE617 pKa = 3.72YY618 pKa = 10.96NIYY621 pKa = 10.75GIVGKK626 pKa = 10.71GIINKK631 pKa = 9.66ANSTLHH637 pKa = 5.56TGIVYY642 pKa = 9.8TDD644 pKa = 3.34TAGVLVSFKK653 pKa = 11.02NVTTGDD659 pKa = 3.48IYY661 pKa = 11.4SVIPCQTSMQYY672 pKa = 11.35AVIADD677 pKa = 4.05NIVGVISADD686 pKa = 3.3TATGITFNHH695 pKa = 7.09TIATPMFYY703 pKa = 10.83YY704 pKa = 9.65STNTDD709 pKa = 3.65RR710 pKa = 11.84NCTEE714 pKa = 3.8PVLTYY719 pKa = 10.29ATMGICADD727 pKa = 3.7GAIGYY732 pKa = 7.2VQPRR736 pKa = 11.84VVSTTPATPITTGNLTIPVNFTVSIQAEE764 pKa = 4.38YY765 pKa = 10.38VQVSLRR771 pKa = 11.84AVVVDD776 pKa = 3.84CATYY780 pKa = 9.6VCNGNVRR787 pKa = 11.84CLQLLRR793 pKa = 11.84QYY795 pKa = 8.82VTACTSVEE803 pKa = 3.79NALALNARR811 pKa = 11.84LEE813 pKa = 4.34SQEE816 pKa = 4.09VADD819 pKa = 4.71MLAVDD824 pKa = 3.84YY825 pKa = 10.79AAYY828 pKa = 10.22RR829 pKa = 11.84SSLEE833 pKa = 3.88LNTPQFEE840 pKa = 4.46NGFNISAVLPAGEE853 pKa = 4.51GKK855 pKa = 10.84GSFIEE860 pKa = 5.32DD861 pKa = 3.63LLFDD865 pKa = 3.9KK866 pKa = 11.22VITNGLGTVDD876 pKa = 4.6ADD878 pKa = 3.57YY879 pKa = 11.06KK880 pKa = 10.91RR881 pKa = 11.84CIEE884 pKa = 4.21EE885 pKa = 3.81KK886 pKa = 10.71GAIADD891 pKa = 3.94VVCRR895 pKa = 11.84QYY897 pKa = 11.77YY898 pKa = 10.12KK899 pKa = 10.81GISVLPALTDD909 pKa = 3.65PARR912 pKa = 11.84MGLYY916 pKa = 9.94SASLMGALTLGAFGGGAVAAPFSIAVFSKK945 pKa = 10.78LNYY948 pKa = 9.39IALQTDD954 pKa = 5.27LIQEE958 pKa = 4.1NQKK961 pKa = 10.71LISAAFNNAMGNITKK976 pKa = 10.55AFTDD980 pKa = 3.55VNTALQHH987 pKa = 6.02VSDD990 pKa = 4.04AVKK993 pKa = 9.61TVATALNKK1001 pKa = 9.86VQDD1004 pKa = 3.84AVNTQGEE1011 pKa = 4.55ALQKK1015 pKa = 9.77LTSQLAQNFDD1025 pKa = 4.76AISSSIDD1032 pKa = 3.65DD1033 pKa = 4.42IYY1035 pKa = 11.93NKK1037 pKa = 10.43LDD1039 pKa = 3.33VLAADD1044 pKa = 3.83AQVDD1048 pKa = 3.7RR1049 pKa = 11.84LINGRR1054 pKa = 11.84LSALSTFVSAQLVKK1068 pKa = 10.62YY1069 pKa = 10.37SEE1071 pKa = 4.51VKK1073 pKa = 10.49ASRR1076 pKa = 11.84NLAMQKK1082 pKa = 10.19VNEE1085 pKa = 4.39CVKK1088 pKa = 10.43SQSSRR1093 pKa = 11.84LGFCGNGTHH1102 pKa = 7.02LFSMVTGAPDD1112 pKa = 3.52GLMFLHH1118 pKa = 6.3TVLLPTEE1125 pKa = 4.26YY1126 pKa = 10.87KK1127 pKa = 10.4EE1128 pKa = 3.97VAAWAGLCVGGKK1140 pKa = 10.25AFVLRR1145 pKa = 11.84DD1146 pKa = 3.52VQLLLFIRR1154 pKa = 11.84IIQYY1158 pKa = 10.87LVTSRR1163 pKa = 11.84NMYY1166 pKa = 9.15QPRR1169 pKa = 11.84VPQMSDD1175 pKa = 2.95FVQIQSCAITYY1186 pKa = 10.33VNLTSEE1192 pKa = 4.64EE1193 pKa = 4.19FNSVVPDD1200 pKa = 3.91YY1201 pKa = 11.33IDD1203 pKa = 3.42VNKK1206 pKa = 9.6TLEE1209 pKa = 4.48DD1210 pKa = 4.0FAATLPNRR1218 pKa = 11.84TYY1220 pKa = 11.2PDD1222 pKa = 3.84FSLDD1226 pKa = 4.0QFNHH1230 pKa = 5.93TYY1232 pKa = 11.21LNISGQISVLEE1243 pKa = 4.11NKK1245 pKa = 9.97SAEE1248 pKa = 4.06LLLITEE1254 pKa = 5.3RR1255 pKa = 11.84LQQHH1259 pKa = 5.86IQNINNSLIDD1269 pKa = 4.32LEE1271 pKa = 4.24WLDD1274 pKa = 4.42RR1275 pKa = 11.84LEE1277 pKa = 5.26TYY1279 pKa = 10.02VKK1281 pKa = 9.41WPWWVWLCFAVVFVILLGLMLWCCIATGCCGCCSCITASCAGCCDD1326 pKa = 3.47CRR1328 pKa = 11.84GKK1330 pKa = 10.56RR1331 pKa = 11.84LQRR1334 pKa = 11.84YY1335 pKa = 6.61EE1336 pKa = 3.78VEE1338 pKa = 4.83KK1339 pKa = 10.91IHH1341 pKa = 6.56IQQ1343 pKa = 3.3

Molecular weight: 147.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1WHG1|A0A0U1WHG1_9ALPC 3C-like proteinase OS=BtNv-AlphaCoV/SC2013 OX=1503291 PE=3 SV=1
MM1 pKa = 7.5SSSKK5 pKa = 11.24GNVGFDD11 pKa = 2.82NAARR15 pKa = 11.84GRR17 pKa = 11.84SGRR20 pKa = 11.84VPFSFYY26 pKa = 10.58MPVINNSSQPFYY38 pKa = 11.42KK39 pKa = 10.64VMPQNAVPKK48 pKa = 10.12GQGNKK53 pKa = 9.62DD54 pKa = 3.41QQIGYY59 pKa = 9.11WNEE62 pKa = 3.54QVRR65 pKa = 11.84WRR67 pKa = 11.84MVKK70 pKa = 8.84GTRR73 pKa = 11.84KK74 pKa = 9.86DD75 pKa = 4.25LPSKK79 pKa = 8.43WHH81 pKa = 6.58FYY83 pKa = 10.71YY84 pKa = 10.91LGTGPHH90 pKa = 6.95ADD92 pKa = 2.84LKK94 pKa = 10.73FRR96 pKa = 11.84QRR98 pKa = 11.84QQGVFWVAKK107 pKa = 9.07EE108 pKa = 3.93GAKK111 pKa = 10.44AEE113 pKa = 4.07PTGLGTRR120 pKa = 11.84GRR122 pKa = 11.84NAEE125 pKa = 3.99LTTPIFNPGLPDD137 pKa = 3.77SIEE140 pKa = 4.01IVDD143 pKa = 3.7QYY145 pKa = 11.8SRR147 pKa = 11.84PNSRR151 pKa = 11.84ASSRR155 pKa = 11.84ARR157 pKa = 11.84SQSNDD162 pKa = 2.8QGNRR166 pKa = 11.84SRR168 pKa = 11.84SQSNNRR174 pKa = 11.84AQSNNRR180 pKa = 11.84SQSRR184 pKa = 11.84GRR186 pKa = 11.84QNQNQNNQPTGDD198 pKa = 3.75GGSNGQRR205 pKa = 11.84NQPRR209 pKa = 11.84NRR211 pKa = 11.84SNSRR215 pKa = 11.84NRR217 pKa = 11.84SGNQGRR223 pKa = 11.84NGGSQQDD230 pKa = 3.39LVAAVRR236 pKa = 11.84EE237 pKa = 4.29ALAGMGFKK245 pKa = 10.69PNTSGSGRR253 pKa = 11.84NTPVKK258 pKa = 10.27VPKK261 pKa = 10.25GDD263 pKa = 3.51KK264 pKa = 9.99PLNKK268 pKa = 8.24PTKK271 pKa = 9.61APASQVEE278 pKa = 4.24KK279 pKa = 9.98PVWKK283 pKa = 8.87RR284 pKa = 11.84TPHH287 pKa = 4.91SQEE290 pKa = 3.68NVEE293 pKa = 4.42VCFGPRR299 pKa = 11.84DD300 pKa = 3.64TYY302 pKa = 11.68QNFGDD307 pKa = 4.02SQLVRR312 pKa = 11.84LGVDD316 pKa = 3.37YY317 pKa = 10.33PHH319 pKa = 6.77YY320 pKa = 9.41PQIGEE325 pKa = 5.1LIPSQAALLFGSEE338 pKa = 3.81ITAHH342 pKa = 5.69EE343 pKa = 4.6RR344 pKa = 11.84GDD346 pKa = 4.15NIQLTYY352 pKa = 9.36IYY354 pKa = 10.7KK355 pKa = 8.98MEE357 pKa = 4.0VPKK360 pKa = 10.67DD361 pKa = 3.88HH362 pKa = 7.17KK363 pKa = 11.01SLAAFLPHH371 pKa = 6.55IGAYY375 pKa = 9.99ADD377 pKa = 3.5STEE380 pKa = 5.07DD381 pKa = 3.27VTLPPALPPKK391 pKa = 7.99QQRR394 pKa = 11.84LRR396 pKa = 11.84RR397 pKa = 11.84SASTEE402 pKa = 3.74VLADD406 pKa = 3.48TTSVVDD412 pKa = 3.63EE413 pKa = 4.71EE414 pKa = 4.44EE415 pKa = 4.32VEE417 pKa = 4.36EE418 pKa = 4.5VVDD421 pKa = 5.25DD422 pKa = 3.74VTGQDD427 pKa = 3.01EE428 pKa = 4.52TFAA431 pKa = 5.66

Molecular weight: 47.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

8882

75

6598

1480.3

164.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.71 ± 0.244

3.445 ± 0.8

5.562 ± 0.68

3.67 ± 0.431

5.663 ± 0.641

6.834 ± 0.433

1.959 ± 0.149

4.447 ± 0.918

4.976 ± 0.854

8.759 ± 0.598

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.027 ± 0.288

5.506 ± 0.504

3.637 ± 0.628

2.984 ± 0.874

3.839 ± 0.807

7.329 ± 0.324

6.192 ± 0.589

10.493 ± 0.722

1.317 ± 0.266

4.65 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski