Human associated cyclovirus 1 (isolate Homo sapiens/Pakistan/PK5510/2007) (HuCyV-1) (Cyclovirus PK5510)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Cyclovirus; Human associated cyclovirus 1

Average proteome isoelectric point is 8.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|D4N3P3|CAPSD_HCYV5 Capsid protein OS=Human associated cyclovirus 1 (isolate Homo sapiens/Pakistan/PK5510/2007) OX=742918 GN=Cap PE=3 SV=1
MM1 pKa = 7.38SNSTVRR7 pKa = 11.84RR8 pKa = 11.84FCFTWNNYY16 pKa = 8.05TEE18 pKa = 5.06LNYY21 pKa = 10.86ALCQEE26 pKa = 5.14FIKK29 pKa = 10.42KK30 pKa = 7.9YY31 pKa = 9.24CKK33 pKa = 10.01YY34 pKa = 10.37GIVGKK39 pKa = 10.07EE40 pKa = 3.76LAPTTNTPHH49 pKa = 6.74LQGFCNLQKK58 pKa = 10.56PMRR61 pKa = 11.84FSTIKK66 pKa = 10.18KK67 pKa = 9.42RR68 pKa = 11.84LDD70 pKa = 3.25NGIHH74 pKa = 6.27IEE76 pKa = 3.9KK77 pKa = 10.89SMGSDD82 pKa = 3.28TQNQTYY88 pKa = 9.73CSKK91 pKa = 10.76SGEE94 pKa = 4.14FFEE97 pKa = 6.59AGDD100 pKa = 3.94PQCQGKK106 pKa = 10.38RR107 pKa = 11.84NDD109 pKa = 3.68LQSVVDD115 pKa = 4.81TIQAGNGSLSSIANEE130 pKa = 4.23HH131 pKa = 5.04PTAYY135 pKa = 9.28IRR137 pKa = 11.84YY138 pKa = 7.99FRR140 pKa = 11.84GIQEE144 pKa = 4.25YY145 pKa = 9.66IKK147 pKa = 9.02TVRR150 pKa = 11.84PIPPRR155 pKa = 11.84YY156 pKa = 9.07HH157 pKa = 5.28KK158 pKa = 9.86TEE160 pKa = 3.2VRR162 pKa = 11.84YY163 pKa = 8.98YY164 pKa = 10.09HH165 pKa = 6.81GPPGSGKK172 pKa = 8.61SRR174 pKa = 11.84RR175 pKa = 11.84ALEE178 pKa = 4.0EE179 pKa = 3.77ATALASDD186 pKa = 4.84LNDD189 pKa = 2.77IYY191 pKa = 11.34YY192 pKa = 10.39KK193 pKa = 10.63PRR195 pKa = 11.84GTWWDD200 pKa = 3.84GYY202 pKa = 10.05KK203 pKa = 9.86QQSCVIIDD211 pKa = 4.9DD212 pKa = 4.45FYY214 pKa = 11.76GWIKK218 pKa = 9.81YY219 pKa = 10.71DD220 pKa = 3.92EE221 pKa = 4.17MLKK224 pKa = 9.91ICDD227 pKa = 3.53RR228 pKa = 11.84YY229 pKa = 9.77PYY231 pKa = 10.1KK232 pKa = 10.94VQIKK236 pKa = 10.43GGFEE240 pKa = 4.14EE241 pKa = 4.92FTSKK245 pKa = 10.99YY246 pKa = 9.93IWITSNIDD254 pKa = 3.02TNLLYY259 pKa = 10.79KK260 pKa = 10.82FNDD263 pKa = 3.53YY264 pKa = 11.34NDD266 pKa = 3.56TAFVRR271 pKa = 11.84RR272 pKa = 11.84IEE274 pKa = 4.02IKK276 pKa = 10.79LLIEE280 pKa = 4.14

Molecular weight: 32.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|D4N3P3|CAPSD_HCYV5 Capsid protein OS=Human associated cyclovirus 1 (isolate Homo sapiens/Pakistan/PK5510/2007) OX=742918 GN=Cap PE=3 SV=1
MM1 pKa = 7.52PFSRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84VVRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 8.54PVRR17 pKa = 11.84RR18 pKa = 11.84LRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84FFKK28 pKa = 10.43RR29 pKa = 11.84AGRR32 pKa = 11.84GSFRR36 pKa = 11.84VKK38 pKa = 9.6LTRR41 pKa = 11.84FVSITQDD48 pKa = 2.64ISKK51 pKa = 7.04TTQFSFEE58 pKa = 4.04ITPSDD63 pKa = 3.44FTEE66 pKa = 4.42FATLADD72 pKa = 3.39AFEE75 pKa = 4.46AVRR78 pKa = 11.84FTRR81 pKa = 11.84AKK83 pKa = 9.56VTVLPQQNVSNNSTSLIPAYY103 pKa = 10.98CMFPWHH109 pKa = 6.92KK110 pKa = 9.99EE111 pKa = 3.91LPTVATFNGFLSIDD125 pKa = 3.41RR126 pKa = 11.84AKK128 pKa = 10.56CFRR131 pKa = 11.84GTQVGIQHH139 pKa = 6.07YY140 pKa = 10.21VPSTLDD146 pKa = 3.49SIQTKK151 pKa = 9.73GAAGYY156 pKa = 8.47EE157 pKa = 4.3VQSVQTKK164 pKa = 8.83YY165 pKa = 10.87KK166 pKa = 8.88PTIQISGGASTVVLYY181 pKa = 10.03TGALGMQALSDD192 pKa = 3.82APEE195 pKa = 4.19NAQAHH200 pKa = 5.09YY201 pKa = 10.25NIKK204 pKa = 9.91IDD206 pKa = 3.47MWCTFINQTSFNKK219 pKa = 10.34

Molecular weight: 25.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

499

219

280

249.5

28.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.611 ± 1.32

2.204 ± 0.512

4.409 ± 0.744

4.409 ± 1.024

6.012 ± 1.074

6.012 ± 0.607

1.603 ± 0.143

7.014 ± 0.942

6.814 ± 0.819

5.411 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.603 ± 0.137

4.81 ± 0.71

4.409 ± 0.097

5.21 ± 0.445

7.816 ± 1.649

6.814 ± 0.582

8.016 ± 0.965

5.01 ± 1.409

1.403 ± 0.3

5.411 ± 1.639
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski