Escherichia phage St11Ph5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; unclassified Gamaleyavirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D2W342|A0A2D2W342_9CAUD Uncharacterized protein OS=Escherichia phage St11Ph5 OX=2047765 GN=St11Ph5_00005 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84FYY4 pKa = 11.46SLVALSTSCYY14 pKa = 10.06LSTVTKK20 pKa = 10.31QDD22 pKa = 3.71CLNGKK27 pKa = 9.56LDD29 pKa = 3.69GTTILYY35 pKa = 8.59EE36 pKa = 4.38PNEE39 pKa = 4.15EE40 pKa = 3.69ADD42 pKa = 4.09EE43 pKa = 4.95FIGTCLMLGIPITFIKK59 pKa = 10.03IDD61 pKa = 3.49TSVEE65 pKa = 3.36ISEE68 pKa = 4.31FARR71 pKa = 11.84VYY73 pKa = 10.35ILSTAEE79 pKa = 3.87PMVVTTLTGRR89 pKa = 11.84LITLYY94 pKa = 10.11PSTSYY99 pKa = 11.09LSSDD103 pKa = 3.22EE104 pKa = 4.34CALL107 pKa = 3.82

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D2W320|A0A2D2W320_9CAUD Tail fiber protein OS=Escherichia phage St11Ph5 OX=2047765 GN=St11Ph5_00080 PE=4 SV=1
MM1 pKa = 7.33VRR3 pKa = 11.84RR4 pKa = 11.84LNRR7 pKa = 11.84KK8 pKa = 9.1VEE10 pKa = 4.16LVPNWKK16 pKa = 9.95RR17 pKa = 11.84CWKK20 pKa = 8.21WASIQISTLGLIMFSAIDD38 pKa = 4.26IIQPMLTGLPRR49 pKa = 11.84HH50 pKa = 6.0ILEE53 pKa = 5.57DD54 pKa = 3.56IPHH57 pKa = 7.03GSNIAIALFALNIIGRR73 pKa = 11.84LIRR76 pKa = 11.84FRR78 pKa = 11.84PKK80 pKa = 10.29EE81 pKa = 4.06DD82 pKa = 3.18SHH84 pKa = 7.49EE85 pKa = 4.23SS86 pKa = 3.3

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

22334

43

3595

250.9

27.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.579 ± 0.594

0.94 ± 0.17

5.852 ± 0.159

6.577 ± 0.272

3.604 ± 0.163

6.613 ± 0.258

1.782 ± 0.145

6.0 ± 0.278

6.515 ± 0.292

8.315 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.892 ± 0.124

5.731 ± 0.164

4.048 ± 0.147

4.124 ± 0.295

4.424 ± 0.148

6.045 ± 0.217

6.251 ± 0.254

6.797 ± 0.246

1.137 ± 0.119

3.775 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski