Nodularia phage vB_NspS-kac65v151

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ravarandavirus; Nodularia virus kac65v151

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 206 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482MIW1|A0A482MIW1_9CAUD Uncharacterized protein OS=Nodularia phage vB_NspS-kac65v151 OX=2557579 GN=kac65v151_gp080 PE=4 SV=1
MM1 pKa = 7.89EE2 pKa = 5.04EE3 pKa = 3.76LQIAKK8 pKa = 8.52EE9 pKa = 3.73ICEE12 pKa = 4.44EE13 pKa = 4.11YY14 pKa = 10.37MSDD17 pKa = 3.41GFAGAGNADD26 pKa = 3.71PVVCFYY32 pKa = 10.99IKK34 pKa = 10.22IGNLVV39 pKa = 3.07

Molecular weight: 4.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482MHF1|A0A482MHF1_9CAUD Uncharacterized protein OS=Nodularia phage vB_NspS-kac65v151 OX=2557579 GN=kac65v151_gp194 PE=4 SV=1
MM1 pKa = 7.29GVMGTRR7 pKa = 11.84FKK9 pKa = 11.11FIFWHH14 pKa = 5.67NQTPYY19 pKa = 10.53IDD21 pKa = 3.61TPRR24 pKa = 11.84PKK26 pKa = 10.58GAGILGSTSPLRR38 pKa = 11.84LDD40 pKa = 3.26SLRR43 pKa = 11.84YY44 pKa = 8.22LTQRR48 pKa = 11.84WFSPQALTFPLPGGSCVTANFVV70 pKa = 3.22

Molecular weight: 7.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

206

0

206

43981

22

3282

213.5

23.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.242 ± 0.233

1.187 ± 0.116

5.868 ± 0.141

6.678 ± 0.211

3.893 ± 0.171

6.157 ± 0.181

1.517 ± 0.14

6.228 ± 0.151

7.299 ± 0.292

9.309 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.773 ± 0.117

4.907 ± 0.144

4.129 ± 0.166

4.661 ± 0.281

4.668 ± 0.124

7.265 ± 0.191

6.228 ± 0.196

6.444 ± 0.131

1.103 ± 0.095

3.447 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski