Tobacco streak virus (strain WC) (TSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus; Tobacco streak virus

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P89679|2B_TOBSV Suppressor of silencing 2b OS=Tobacco streak virus (strain WC) OX=12318 GN=ORF2b PE=3 SV=1
MM1 pKa = 7.83DD2 pKa = 5.53LVIKK6 pKa = 10.56NLIVYY11 pKa = 8.55HH12 pKa = 5.57LRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84IDD18 pKa = 2.76IGTSFGIEE26 pKa = 3.65PADD29 pKa = 3.82YY30 pKa = 10.41IDD32 pKa = 3.67WVKK35 pKa = 10.78VFLLKK40 pKa = 10.42FIIEE44 pKa = 4.12HH45 pKa = 5.26TARR48 pKa = 11.84FADD51 pKa = 4.68FATIHH56 pKa = 5.02TTMLLVLGEE65 pKa = 4.74DD66 pKa = 3.58DD67 pKa = 3.88PNYY70 pKa = 10.36VEE72 pKa = 5.19KK73 pKa = 9.84DD74 pKa = 3.61TPIMEE79 pKa = 4.98IDD81 pKa = 4.14PFYY84 pKa = 11.2LPYY87 pKa = 10.75DD88 pKa = 4.42DD89 pKa = 6.34LDD91 pKa = 3.39VDD93 pKa = 4.07YY94 pKa = 10.73TSLRR98 pKa = 11.84VCGDD102 pKa = 3.42EE103 pKa = 5.19DD104 pKa = 3.93QSCSDD109 pKa = 4.01RR110 pKa = 11.84DD111 pKa = 3.67EE112 pKa = 5.07LSDD115 pKa = 4.08FISNISHH122 pKa = 6.27IPEE125 pKa = 4.21GTSWGSEE132 pKa = 3.59SDD134 pKa = 3.47TSFVEE139 pKa = 4.1HH140 pKa = 7.24LEE142 pKa = 4.41TIQDD146 pKa = 3.34IPTKK150 pKa = 10.76CEE152 pKa = 3.63IADD155 pKa = 3.93KK156 pKa = 10.05PVEE159 pKa = 4.77EE160 pKa = 5.36IPFDD164 pKa = 4.63DD165 pKa = 5.22DD166 pKa = 4.06GKK168 pKa = 10.75VVNDD172 pKa = 3.18VWVDD176 pKa = 3.61AEE178 pKa = 4.56LSNAPEE184 pKa = 4.1ISCDD188 pKa = 3.28ADD190 pKa = 2.89IRR192 pKa = 11.84ACGFVSLRR200 pKa = 11.84LLEE203 pKa = 4.17SSRR206 pKa = 11.84GYY208 pKa = 9.84PKK210 pKa = 8.65WTPEE214 pKa = 3.93RR215 pKa = 11.84VSSGLNPDD223 pKa = 3.99LPVNSKK229 pKa = 9.96PAVDD233 pKa = 4.71EE234 pKa = 4.31IFPHH238 pKa = 5.7HH239 pKa = 6.92HH240 pKa = 6.28SVDD243 pKa = 3.3DD244 pKa = 4.14RR245 pKa = 11.84FFQEE249 pKa = 3.7WVEE252 pKa = 3.99THH254 pKa = 7.34DD255 pKa = 3.95IDD257 pKa = 6.24LEE259 pKa = 4.47VTSCDD264 pKa = 3.8LDD266 pKa = 3.23MSTFNDD272 pKa = 3.13WTKK275 pKa = 11.11GVDD278 pKa = 3.3TRR280 pKa = 11.84LVPNMSVGGLSHH292 pKa = 7.1RR293 pKa = 11.84VPTQRR298 pKa = 11.84EE299 pKa = 3.62ALLAIKK305 pKa = 9.37KK306 pKa = 10.3RR307 pKa = 11.84NMNVPEE313 pKa = 4.11LQSNFDD319 pKa = 3.87HH320 pKa = 7.95DD321 pKa = 5.07DD322 pKa = 3.38VLNRR326 pKa = 11.84CVTRR330 pKa = 11.84FITHH334 pKa = 5.6VVDD337 pKa = 3.52KK338 pKa = 10.93TRR340 pKa = 11.84LSKK343 pKa = 10.94LNPISGEE350 pKa = 3.79EE351 pKa = 3.48LHH353 pKa = 6.79YY354 pKa = 10.77FNQYY358 pKa = 10.65LEE360 pKa = 4.47NKK362 pKa = 9.44NPPLSEE368 pKa = 4.1YY369 pKa = 10.16KK370 pKa = 10.95GPVPLVALDD379 pKa = 4.49KK380 pKa = 10.26YY381 pKa = 9.66MHH383 pKa = 6.57MIKK386 pKa = 7.75TTLKK390 pKa = 10.38PVEE393 pKa = 4.37EE394 pKa = 4.96DD395 pKa = 3.04NLHH398 pKa = 6.21IEE400 pKa = 4.23RR401 pKa = 11.84PIPATITYY409 pKa = 8.34HH410 pKa = 5.89KK411 pKa = 9.69KK412 pKa = 9.51GVVMMTSPYY421 pKa = 9.61FLCAMVRR428 pKa = 11.84LLYY431 pKa = 10.08VLKK434 pKa = 10.74SKK436 pKa = 10.49FVVPTGKK443 pKa = 9.74YY444 pKa = 9.12HH445 pKa = 7.59QIFQMNPEE453 pKa = 4.33LLKK456 pKa = 10.62HH457 pKa = 5.58SKK459 pKa = 8.92EE460 pKa = 3.97FKK462 pKa = 10.52EE463 pKa = 3.83IDD465 pKa = 3.34FSKK468 pKa = 10.51FDD470 pKa = 3.37KK471 pKa = 11.04SQGRR475 pKa = 11.84LHH477 pKa = 7.01HH478 pKa = 6.99DD479 pKa = 3.25VQFRR483 pKa = 11.84LFLALGIPEE492 pKa = 4.7HH493 pKa = 6.97FVTTWFNSHH502 pKa = 5.67EE503 pKa = 4.0KK504 pKa = 10.58SYY506 pKa = 10.97IRR508 pKa = 11.84DD509 pKa = 3.57RR510 pKa = 11.84DD511 pKa = 3.73CGIGFSVDD519 pKa = 3.25YY520 pKa = 10.46QRR522 pKa = 11.84RR523 pKa = 11.84TGDD526 pKa = 2.74ACTYY530 pKa = 10.46LGNTLVTLSVLSYY543 pKa = 11.16VYY545 pKa = 10.73DD546 pKa = 4.12LSNPNILFVAASGDD560 pKa = 3.57DD561 pKa = 3.67SLIGSIEE568 pKa = 3.73PLPRR572 pKa = 11.84EE573 pKa = 4.19KK574 pKa = 10.83EE575 pKa = 4.13DD576 pKa = 3.47LCVSLFNFEE585 pKa = 4.08TKK587 pKa = 10.2FPHH590 pKa = 5.6NQPFICSKK598 pKa = 9.9FLLVVEE604 pKa = 4.98CDD606 pKa = 3.73DD607 pKa = 4.32GSEE610 pKa = 4.08EE611 pKa = 4.15VLAVPNPLKK620 pKa = 10.61LLQKK624 pKa = 10.54LGPKK628 pKa = 9.39NLQVTVLDD636 pKa = 4.97DD637 pKa = 4.31YY638 pKa = 11.44YY639 pKa = 11.6QSLCDD644 pKa = 3.84ILWVFNDD651 pKa = 3.15ADD653 pKa = 3.74VCRR656 pKa = 11.84RR657 pKa = 11.84TAEE660 pKa = 3.76LAEE663 pKa = 3.78YY664 pKa = 10.54RR665 pKa = 11.84RR666 pKa = 11.84FKK668 pKa = 9.84GTKK671 pKa = 8.33KK672 pKa = 10.63CLFLEE677 pKa = 4.46SALLSLPSLVANRR690 pKa = 11.84MKK692 pKa = 10.58FVRR695 pKa = 11.84RR696 pKa = 11.84TINLEE701 pKa = 3.62SSRR704 pKa = 11.84ACIRR708 pKa = 11.84NDD710 pKa = 3.14VYY712 pKa = 11.68SDD714 pKa = 3.93LVPHH718 pKa = 6.61FDD720 pKa = 4.21SRR722 pKa = 11.84VSRR725 pKa = 11.84CDD727 pKa = 3.36DD728 pKa = 3.32SDD730 pKa = 3.84GVRR733 pKa = 11.84TSTFDD738 pKa = 3.27DD739 pKa = 3.77RR740 pKa = 11.84KK741 pKa = 9.49SSKK744 pKa = 10.14HH745 pKa = 6.32ASDD748 pKa = 3.6KK749 pKa = 10.83LRR751 pKa = 11.84KK752 pKa = 7.13TEE754 pKa = 4.07CYY756 pKa = 10.54GEE758 pKa = 3.97ARR760 pKa = 11.84CRR762 pKa = 11.84IKK764 pKa = 10.41PRR766 pKa = 11.84RR767 pKa = 11.84NRR769 pKa = 11.84KK770 pKa = 8.99SEE772 pKa = 4.03SGAVQYY778 pKa = 10.3SQSSGIEE785 pKa = 3.72TGKK788 pKa = 10.81ANSSRR793 pKa = 11.84KK794 pKa = 9.77GRR796 pKa = 11.84IKK798 pKa = 10.7LHH800 pKa = 5.83

Molecular weight: 91.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P89678|RDRP_TOBSV RNA-directed RNA polymerase 2a OS=Tobacco streak virus (strain WC) OX=12318 GN=ORF2a PE=3 SV=1
MM1 pKa = 6.93NTLIQGPDD9 pKa = 3.26HH10 pKa = 7.37PSNAMSSRR18 pKa = 11.84ANNRR22 pKa = 11.84SNNSRR27 pKa = 11.84CPTCIDD33 pKa = 4.0EE34 pKa = 4.81LDD36 pKa = 3.45AMARR40 pKa = 11.84NCPAHH45 pKa = 5.55NTVNTVSRR53 pKa = 11.84RR54 pKa = 11.84QRR56 pKa = 11.84RR57 pKa = 11.84NAARR61 pKa = 11.84AAAYY65 pKa = 9.79RR66 pKa = 11.84NANARR71 pKa = 11.84VPLPLPVVSVSRR83 pKa = 11.84PQAKK87 pKa = 10.2ASLRR91 pKa = 11.84LPNNQVWVTRR101 pKa = 11.84KK102 pKa = 9.79ASEE105 pKa = 3.9WSAKK109 pKa = 9.28TVDD112 pKa = 3.85TNDD115 pKa = 4.54AIPFKK120 pKa = 10.15TIVEE124 pKa = 4.91GIPEE128 pKa = 4.17IGAEE132 pKa = 4.19TKK134 pKa = 10.45FFRR137 pKa = 11.84LLIGFVAVSDD147 pKa = 4.06GTFGMVDD154 pKa = 3.6GVTGDD159 pKa = 4.29VIPDD163 pKa = 3.61PPVVGRR169 pKa = 11.84LGFKK173 pKa = 10.51KK174 pKa = 9.03NTYY177 pKa = 9.52RR178 pKa = 11.84SRR180 pKa = 11.84DD181 pKa = 3.17FDD183 pKa = 4.71LGGKK187 pKa = 9.19LLNQLDD193 pKa = 3.73DD194 pKa = 4.66RR195 pKa = 11.84AVVWCLDD202 pKa = 3.29EE203 pKa = 4.46RR204 pKa = 11.84RR205 pKa = 11.84RR206 pKa = 11.84EE207 pKa = 4.1AKK209 pKa = 9.57RR210 pKa = 11.84VQLAGYY216 pKa = 8.3WIAISKK222 pKa = 7.65PAPLMPPEE230 pKa = 5.11DD231 pKa = 3.6FLVNQDD237 pKa = 3.07

Molecular weight: 26.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2625

205

1094

525.0

59.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.248 ± 1.081

2.286 ± 0.264

6.933 ± 0.839

6.095 ± 0.351

4.305 ± 0.334

4.533 ± 0.282

2.743 ± 0.46

5.905 ± 0.388

6.248 ± 0.345

9.105 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.368

4.305 ± 0.442

5.181 ± 0.777

2.324 ± 0.129

5.79 ± 0.683

8.114 ± 0.366

5.79 ± 0.644

8.038 ± 0.298

0.952 ± 0.112

2.59 ± 0.518
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski