Moraxella macacae 0408225
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1822 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L2F5K3|L2F5K3_9GAMM Serine acetyltransferase OS=Moraxella macacae 0408225 OX=1230338 GN=MOMA_07271 PE=3 SV=1
MM1 pKa = 7.57 SNTPADD7 pKa = 3.95 LKK9 pKa = 11.14 YY10 pKa = 10.44 VASHH14 pKa = 5.26 EE15 pKa = 4.22 WLRR18 pKa = 11.84 QEE20 pKa = 3.75 SDD22 pKa = 3.33 GVITVGITHH31 pKa = 7.56 HH32 pKa = 6.55 AQDD35 pKa = 3.87 LLGDD39 pKa = 3.68 VVYY42 pKa = 10.77 IEE44 pKa = 4.51 LPEE47 pKa = 4.19 VGTNVEE53 pKa = 3.83 VDD55 pKa = 3.19 AEE57 pKa = 4.26 IAVVEE62 pKa = 4.47 SVKK65 pKa = 10.67 AASDD69 pKa = 3.76 VYY71 pKa = 11.37 APIAGEE77 pKa = 3.64 IVEE80 pKa = 4.77 INDD83 pKa = 3.8 ALVDD87 pKa = 3.58 TPEE90 pKa = 4.39 IVNSDD95 pKa = 3.73 PYY97 pKa = 11.02 GEE99 pKa = 4.2 GWFFKK104 pKa = 10.39 IKK106 pKa = 8.48 PTNASDD112 pKa = 3.91 YY113 pKa = 10.85 DD114 pKa = 3.78 GLMDD118 pKa = 5.05 ADD120 pKa = 4.9 EE121 pKa = 4.77 YY122 pKa = 11.44 LDD124 pKa = 4.5 SII126 pKa = 4.97
Molecular weight: 13.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.77
IPC_protein 3.745
Toseland 3.541
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.859
Patrickios 1.863
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|L2F854|L2F854_9GAMM Phosphoglycolate phosphatase OS=Moraxella macacae 0408225 OX=1230338 GN=MOMA_01585 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.81 IKK11 pKa = 10.24 RR12 pKa = 11.84 KK13 pKa = 7.73 RR14 pKa = 11.84 THH16 pKa = 5.96 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.03 GRR39 pKa = 11.84 HH40 pKa = 5.34 RR41 pKa = 11.84 LTVV44 pKa = 3.07
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1822
0
1822
599093
32
2065
328.8
36.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.233 ± 0.059
0.962 ± 0.022
5.758 ± 0.042
4.881 ± 0.051
4.238 ± 0.037
6.315 ± 0.057
2.351 ± 0.031
6.773 ± 0.037
6.409 ± 0.054
10.418 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.55 ± 0.028
5.691 ± 0.057
3.835 ± 0.03
5.339 ± 0.061
3.896 ± 0.043
5.667 ± 0.038
5.938 ± 0.043
6.628 ± 0.054
1.067 ± 0.024
3.05 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here