Kroppenstedtia eburnea

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Kroppenstedtia

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3484 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7KW18|A0A1N7KW18_9BACL Competence protein ComEA OS=Kroppenstedtia eburnea OX=714067 GN=SAMN05421790_103304 PE=4 SV=1
MM1 pKa = 7.67ARR3 pKa = 11.84MLCKK7 pKa = 10.35CGEE10 pKa = 4.32TLSTVQVPNEE20 pKa = 3.75IEE22 pKa = 4.21LYY24 pKa = 10.27VYY26 pKa = 10.61TDD28 pKa = 3.6FEE30 pKa = 4.26MDD32 pKa = 4.62EE33 pKa = 4.24INAMDD38 pKa = 4.64INDD41 pKa = 4.37PMDD44 pKa = 3.75IPDD47 pKa = 4.12PEE49 pKa = 4.61RR50 pKa = 11.84DD51 pKa = 3.24VWRR54 pKa = 11.84CPHH57 pKa = 6.64CEE59 pKa = 3.71RR60 pKa = 11.84IYY62 pKa = 11.31VFDD65 pKa = 4.06GNKK68 pKa = 9.86VIKK71 pKa = 9.67TYY73 pKa = 10.65VLEE76 pKa = 4.25EE77 pKa = 4.42DD78 pKa = 3.92EE79 pKa = 4.7EE80 pKa = 4.6EE81 pKa = 4.35EE82 pKa = 4.75NGGNN86 pKa = 3.74

Molecular weight: 10.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7LXB9|A0A1N7LXB9_9BACL 2-isopropylmalate synthase OS=Kroppenstedtia eburnea OX=714067 GN=leuA PE=3 SV=1
MM1 pKa = 7.07QGWNRR6 pKa = 11.84ASRR9 pKa = 11.84FAQQSFALWVLVFAGIAWMFPEE31 pKa = 4.6IFQWIGPAITPLLGIIMFGMGLTLKK56 pKa = 10.68GADD59 pKa = 3.44FRR61 pKa = 11.84LVFQRR66 pKa = 11.84PGPVLAGVCLQFIIMPATAWIVATALGLPPEE97 pKa = 4.49LAAGVMLVGACPGGTASNVIVYY119 pKa = 9.41LSKK122 pKa = 11.25GDD124 pKa = 3.69VPLSVTMTSISTLLAPLLTPFLLWWLAGSWLPVDD158 pKa = 4.39PGSMVLSILQVVILPIVLGLLVRR181 pKa = 11.84RR182 pKa = 11.84FFPVTVRR189 pKa = 11.84RR190 pKa = 11.84ATEE193 pKa = 4.0ALPLISVTAIVIIVAGVVALNADD216 pKa = 4.09SLGKK220 pKa = 8.21TALPVILAVILHH232 pKa = 6.42NGLGLLLGYY241 pKa = 9.19WCARR245 pKa = 11.84ALRR248 pKa = 11.84LDD250 pKa = 3.82EE251 pKa = 4.48PRR253 pKa = 11.84SRR255 pKa = 11.84AVSIEE260 pKa = 3.55VGMQNSGLAVALALAHH276 pKa = 6.62LNPIAALPGAIFSVWHH292 pKa = 6.48NISGSLLATYY302 pKa = 9.27WSRR305 pKa = 11.84KK306 pKa = 8.16GRR308 pKa = 11.84AKK310 pKa = 9.75TRR312 pKa = 11.84PTQAATGG319 pKa = 3.71

Molecular weight: 33.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3484

0

3484

997009

27

3165

286.2

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.744 ± 0.041

0.77 ± 0.012

5.015 ± 0.031

7.558 ± 0.058

3.858 ± 0.031

8.238 ± 0.041

2.319 ± 0.019

5.848 ± 0.04

5.138 ± 0.043

10.404 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.7 ± 0.019

2.902 ± 0.03

4.671 ± 0.034

3.715 ± 0.029

6.752 ± 0.047

5.426 ± 0.026

5.134 ± 0.032

7.564 ± 0.037

1.409 ± 0.02

2.833 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski