Olive latent virus 1
Average proteome isoelectric point is 8.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q83941|Q83941_9TOMB p6 OS=Olive latent virus 1 OX=47669 PE=4 SV=1
MM1 pKa = 8.39 DD2 pKa = 3.84 YY3 pKa = 9.22 QTEE6 pKa = 4.13 VSEE9 pKa = 5.25 DD10 pKa = 3.54 NVSVRR15 pKa = 11.84 GRR17 pKa = 11.84 ARR19 pKa = 11.84 RR20 pKa = 11.84 GTEE23 pKa = 3.88 DD24 pKa = 3.32 KK25 pKa = 10.69 KK26 pKa = 10.52 HH27 pKa = 6.16 NGSGLTGVKK36 pKa = 9.46 RR37 pKa = 11.84 HH38 pKa = 5.83 AVSEE42 pKa = 4.39 TSQKK46 pKa = 9.9 SQSRR50 pKa = 11.84 YY51 pKa = 6.17 WSNGTMTNIAEE62 pKa = 4.27 EE63 pKa = 3.98 QTITVTYY70 pKa = 10.55 NFNFF74 pKa = 3.71
Molecular weight: 8.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.76
IPC2_protein 7.497
IPC_protein 8.448
Toseland 8.77
ProMoST 8.009
Dawson 8.77
Bjellqvist 8.112
Wikipedia 8.902
Rodwell 8.756
Grimsley 8.639
Solomon 9.311
Lehninger 9.282
Nozaki 8.273
DTASelect 8.551
Thurlkill 8.595
EMBOSS 9.004
Sillero 8.726
Patrickios 4.8
IPC_peptide 9.297
IPC2_peptide 7.556
IPC2.peptide.svr19 7.802
Protein with the highest isoelectric point:
>tr|Q83940|Q83940_9TOMB Uncharacterized protein OS=Olive latent virus 1 OX=47669 PE=4 SV=1
MM1 pKa = 7.66 EE2 pKa = 6.2 LPNQHH7 pKa = 6.32 KK8 pKa = 8.46 QTAAEE13 pKa = 4.35 GFVSFLNWLCNPWRR27 pKa = 11.84 RR28 pKa = 11.84 QRR30 pKa = 11.84 TVNAAVAFQNSILAIEE46 pKa = 4.52 DD47 pKa = 3.53 VEE49 pKa = 4.23 HH50 pKa = 7.04 FEE52 pKa = 6.3 DD53 pKa = 3.88 INEE56 pKa = 4.14 CFEE59 pKa = 4.24 EE60 pKa = 4.73 SSGGQSQRR68 pKa = 11.84 TKK70 pKa = 10.67 VVAEE74 pKa = 4.2 GAYY77 pKa = 10.54 SPVKK81 pKa = 9.96 SEE83 pKa = 3.46 RR84 pKa = 11.84 TRR86 pKa = 11.84 RR87 pKa = 11.84 VRR89 pKa = 11.84 KK90 pKa = 9.76 QKK92 pKa = 11.08 AKK94 pKa = 9.41 FVKK97 pKa = 10.51 YY98 pKa = 10.14 LVNEE102 pKa = 3.83 ARR104 pKa = 11.84 AEE106 pKa = 4.09 FGLPKK111 pKa = 9.72 ATEE114 pKa = 4.06 ANRR117 pKa = 11.84 LMVQHH122 pKa = 6.67 FLLRR126 pKa = 11.84 RR127 pKa = 11.84 CKK129 pKa = 10.08 DD130 pKa = 2.97 WGVVTSQCHH139 pKa = 4.95 NNVALALTLVFVPTGDD155 pKa = 4.03 DD156 pKa = 3.15 LLARR160 pKa = 11.84 VVMNTYY166 pKa = 8.26 KK167 pKa = 10.13 TRR169 pKa = 11.84 SAVRR173 pKa = 11.84 GMDD176 pKa = 3.52 NLQGEE181 pKa = 4.94 GWWNNRR187 pKa = 11.84 LGIGGQAGLAFRR199 pKa = 11.84 AKK201 pKa = 10.47
Molecular weight: 22.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.981
IPC2_protein 8.785
IPC_protein 8.785
Toseland 9.648
ProMoST 9.414
Dawson 9.838
Bjellqvist 9.575
Wikipedia 9.97
Rodwell 10.204
Grimsley 9.867
Solomon 9.897
Lehninger 9.882
Nozaki 9.823
DTASelect 9.502
Thurlkill 9.721
EMBOSS 10.058
Sillero 9.809
Patrickios 9.75
IPC_peptide 9.897
IPC2_peptide 8.346
IPC2.peptide.svr19 7.721
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1324
56
723
264.8
29.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.722 ± 0.919
1.964 ± 0.219
3.625 ± 0.208
5.514 ± 0.848
4.532 ± 0.197
6.949 ± 0.334
2.417 ± 0.46
4.532 ± 0.788
5.287 ± 0.957
8.233 ± 0.999
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.417 ± 0.133
5.589 ± 0.38
4.607 ± 0.859
5.211 ± 0.1
6.571 ± 0.397
6.495 ± 0.459
6.269 ± 1.094
7.779 ± 0.377
1.586 ± 0.192
3.625 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here