Aestuariivirga litoralis
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3996 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W2ASP7|A0A2W2ASP7_9RHIZ Alpha/beta hydrolase OS=Aestuariivirga litoralis OX=2650924 GN=DK847_12840 PE=4 SV=1
MM1 pKa = 7.61 LYY3 pKa = 10.93 VMSTAASAATLDD15 pKa = 3.9 FTLTTSKK22 pKa = 10.54 PVIVTGTPRR31 pKa = 11.84 LAIDD35 pKa = 3.18 VGGVTRR41 pKa = 11.84 HH42 pKa = 6.08 ADD44 pKa = 3.39 YY45 pKa = 9.21 TAGSGTTALTFSYY58 pKa = 10.9 AVGAGDD64 pKa = 4.39 FDD66 pKa = 5.71 ANGISLVPPLDD77 pKa = 3.96 LNGGSLTDD85 pKa = 3.52 ASGNAVGSLAFTAPDD100 pKa = 3.34 TSGIRR105 pKa = 11.84 IQTYY109 pKa = 8.12 TAAFDD114 pKa = 3.78 TATVTSANAAALSFRR129 pKa = 11.84 IAKK132 pKa = 9.64 APPGGSYY139 pKa = 10.18 SYY141 pKa = 10.96 SITSSGGAGTVTGSGTISASPQVVSGVDD169 pKa = 3.53 VTSLAVGTLTLSVTVSTAAGGTGAARR195 pKa = 11.84 QATAAFDD202 pKa = 3.73 NVAPSGYY209 pKa = 9.75 SAAFLTSPVTSLNSSAAGFEE229 pKa = 4.31 LTGGEE234 pKa = 3.76 IGASYY239 pKa = 10.56 SYY241 pKa = 11.24 AIGSSGGGVPVTGSGTVSADD261 pKa = 3.28 PQQITGLDD269 pKa = 3.64 LSGLGDD275 pKa = 3.66 GTLTLSVTLTDD286 pKa = 3.68 TVGNAGAAATATALKK301 pKa = 9.18 DD302 pKa = 3.53 TASPTIVSVTPPAAGTYY319 pKa = 10.73 DD320 pKa = 3.67 DD321 pKa = 4.77 LL322 pKa = 6.74
Molecular weight: 30.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.592
ProMoST 4.05
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.859
Rodwell 3.656
Grimsley 3.503
Solomon 3.846
Lehninger 3.808
Nozaki 3.986
DTASelect 4.317
Thurlkill 3.681
EMBOSS 3.859
Sillero 3.973
Patrickios 0.998
IPC_peptide 3.846
IPC2_peptide 3.935
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A2W2AME5|A0A2W2AME5_9RHIZ Hemerythrin domain-containing protein OS=Aestuariivirga litoralis OX=2650924 GN=DK847_12205 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3996
0
3996
1285438
18
2121
321.7
34.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.531 ± 0.055
0.934 ± 0.013
5.429 ± 0.029
5.484 ± 0.035
3.731 ± 0.023
8.703 ± 0.036
2.12 ± 0.02
5.095 ± 0.03
3.774 ± 0.037
10.089 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.662 ± 0.018
2.654 ± 0.021
5.291 ± 0.027
3.114 ± 0.022
6.708 ± 0.035
5.249 ± 0.026
5.307 ± 0.03
7.355 ± 0.034
1.449 ± 0.016
2.32 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here