Amino acid dipepetide frequency for Bacteriophage sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.346AlaAla: 10.346 ± 2.164
1.2AlaCys: 1.2 ± 0.481
2.999AlaAsp: 2.999 ± 0.666
3.899AlaGlu: 3.899 ± 0.913
1.949AlaPhe: 1.949 ± 0.638
6.298AlaGly: 6.298 ± 1.382
0.9AlaHis: 0.9 ± 0.326
4.798AlaIle: 4.798 ± 1.318
6.598AlaLys: 6.598 ± 1.243
5.698AlaLeu: 5.698 ± 1.222
1.35AlaMet: 1.35 ± 0.383
2.549AlaAsn: 2.549 ± 0.83
0.6AlaPro: 0.6 ± 0.27
2.249AlaGln: 2.249 ± 0.733
2.099AlaArg: 2.099 ± 0.716
5.548AlaSer: 5.548 ± 1.167
6.148AlaThr: 6.148 ± 1.422
4.948AlaVal: 4.948 ± 0.969
1.05AlaTrp: 1.05 ± 0.388
2.699AlaTyr: 2.699 ± 0.513
0.0AlaXaa: 0.0 ± 0.0
Cys
0.45CysAla: 0.45 ± 0.357
0.9CysCys: 0.9 ± 0.499
1.499CysAsp: 1.499 ± 0.385
1.2CysGlu: 1.2 ± 0.451
0.9CysPhe: 0.9 ± 0.476
0.9CysGly: 0.9 ± 0.288
0.15CysHis: 0.15 ± 0.137
1.2CysIle: 1.2 ± 0.442
1.05CysLys: 1.05 ± 0.349
1.799CysLeu: 1.799 ± 0.779
0.15CysMet: 0.15 ± 0.14
0.45CysAsn: 0.45 ± 0.302
1.2CysPro: 1.2 ± 0.495
1.05CysGln: 1.05 ± 0.553
0.6CysArg: 0.6 ± 0.345
2.399CysSer: 2.399 ± 0.813
1.2CysThr: 1.2 ± 0.482
0.6CysVal: 0.6 ± 0.342
0.45CysTrp: 0.45 ± 0.278
0.6CysTyr: 0.6 ± 0.382
0.0CysXaa: 0.0 ± 0.0
Asp
4.798AspAla: 4.798 ± 0.859
0.9AspCys: 0.9 ± 0.467
2.249AspAsp: 2.249 ± 0.678
6.298AspGlu: 6.298 ± 1.113
2.399AspPhe: 2.399 ± 0.659
5.548AspGly: 5.548 ± 1.12
0.75AspHis: 0.75 ± 0.484
4.798AspIle: 4.798 ± 1.272
4.498AspLys: 4.498 ± 0.897
2.999AspLeu: 2.999 ± 0.694
2.249AspMet: 2.249 ± 0.738
1.949AspAsn: 1.949 ± 0.603
1.35AspPro: 1.35 ± 0.425
1.05AspGln: 1.05 ± 0.391
2.849AspArg: 2.849 ± 0.703
3.149AspSer: 3.149 ± 0.499
4.648AspThr: 4.648 ± 1.096
3.299AspVal: 3.299 ± 0.859
0.3AspTrp: 0.3 ± 0.295
2.399AspTyr: 2.399 ± 0.58
0.0AspXaa: 0.0 ± 0.0
Glu
5.098GluAla: 5.098 ± 1.257
0.6GluCys: 0.6 ± 0.282
3.749GluAsp: 3.749 ± 0.657
7.197GluGlu: 7.197 ± 1.694
3.299GluPhe: 3.299 ± 0.589
3.899GluGly: 3.899 ± 0.733
1.649GluHis: 1.649 ± 0.665
4.648GluIle: 4.648 ± 1.014
7.347GluLys: 7.347 ± 0.949
6.898GluLeu: 6.898 ± 0.94
2.249GluMet: 2.249 ± 0.527
3.599GluAsn: 3.599 ± 0.781
1.649GluPro: 1.649 ± 0.514
5.548GluGln: 5.548 ± 1.443
4.049GluArg: 4.049 ± 1.526
4.199GluSer: 4.199 ± 0.831
4.648GluThr: 4.648 ± 0.674
4.049GluVal: 4.049 ± 0.718
0.9GluTrp: 0.9 ± 0.33
1.799GluTyr: 1.799 ± 0.448
0.0GluXaa: 0.0 ± 0.0
Phe
2.099PheAla: 2.099 ± 0.56
1.499PheCys: 1.499 ± 0.637
3.149PheAsp: 3.149 ± 0.872
3.299PheGlu: 3.299 ± 0.844
1.499PhePhe: 1.499 ± 0.403
2.999PheGly: 2.999 ± 0.866
0.6PheHis: 0.6 ± 0.281
1.2PheIle: 1.2 ± 0.347
2.249PheLys: 2.249 ± 0.584
3.299PheLeu: 3.299 ± 1.066
1.499PheMet: 1.499 ± 0.588
1.649PheAsn: 1.649 ± 0.323
1.649PhePro: 1.649 ± 0.539
0.6PheGln: 0.6 ± 0.326
2.099PheArg: 2.099 ± 0.746
3.899PheSer: 3.899 ± 0.98
2.249PheThr: 2.249 ± 0.73
2.849PheVal: 2.849 ± 0.569
0.6PheTrp: 0.6 ± 0.364
1.799PheTyr: 1.799 ± 0.48
0.0PheXaa: 0.0 ± 0.0
Gly
5.398GlyAla: 5.398 ± 1.43
1.05GlyCys: 1.05 ± 0.446
4.798GlyAsp: 4.798 ± 1.081
3.449GlyGlu: 3.449 ± 0.853
2.399GlyPhe: 2.399 ± 0.547
5.398GlyGly: 5.398 ± 1.175
1.35GlyHis: 1.35 ± 0.515
3.599GlyIle: 3.599 ± 1.213
6.298GlyLys: 6.298 ± 0.869
4.498GlyLeu: 4.498 ± 0.875
1.949GlyMet: 1.949 ± 0.453
2.999GlyAsn: 2.999 ± 0.81
0.15GlyPro: 0.15 ± 0.13
3.149GlyGln: 3.149 ± 1.121
1.799GlyArg: 1.799 ± 0.628
3.599GlySer: 3.599 ± 0.934
4.798GlyThr: 4.798 ± 1.072
3.299GlyVal: 3.299 ± 0.536
1.05GlyTrp: 1.05 ± 0.481
3.899GlyTyr: 3.899 ± 0.821
0.0GlyXaa: 0.0 ± 0.0
His
0.3HisAla: 0.3 ± 0.198
0.3HisCys: 0.3 ± 0.226
0.6HisAsp: 0.6 ± 0.379
1.2HisGlu: 1.2 ± 0.434
1.499HisPhe: 1.499 ± 0.407
0.45HisGly: 0.45 ± 0.242
0.75HisHis: 0.75 ± 0.44
1.35HisIle: 1.35 ± 0.46
1.35HisLys: 1.35 ± 0.457
1.499HisLeu: 1.499 ± 0.567
0.45HisMet: 0.45 ± 0.273
0.75HisAsn: 0.75 ± 0.29
1.05HisPro: 1.05 ± 0.397
0.45HisGln: 0.45 ± 0.296
0.9HisArg: 0.9 ± 0.447
0.9HisSer: 0.9 ± 0.353
0.9HisThr: 0.9 ± 0.347
1.799HisVal: 1.799 ± 0.573
0.0HisTrp: 0.0 ± 0.0
0.45HisTyr: 0.45 ± 0.367
0.0HisXaa: 0.0 ± 0.0
Ile
4.648IleAla: 4.648 ± 0.996
0.45IleCys: 0.45 ± 0.278
3.599IleAsp: 3.599 ± 0.57
4.049IleGlu: 4.049 ± 0.932
1.799IlePhe: 1.799 ± 0.59
2.549IleGly: 2.549 ± 0.584
1.649IleHis: 1.649 ± 0.591
3.449IleIle: 3.449 ± 0.854
5.848IleLys: 5.848 ± 1.005
4.348IleLeu: 4.348 ± 0.838
1.799IleMet: 1.799 ± 0.465
3.149IleAsn: 3.149 ± 0.556
2.249IlePro: 2.249 ± 0.709
1.35IleGln: 1.35 ± 0.45
4.199IleArg: 4.199 ± 0.931
3.449IleSer: 3.449 ± 0.713
3.749IleThr: 3.749 ± 0.706
3.899IleVal: 3.899 ± 0.552
0.45IleTrp: 0.45 ± 0.325
1.799IleTyr: 1.799 ± 0.813
0.0IleXaa: 0.0 ± 0.0
Lys
8.097LysAla: 8.097 ± 1.322
1.35LysCys: 1.35 ± 0.489
4.648LysAsp: 4.648 ± 0.989
7.347LysGlu: 7.347 ± 1.099
2.099LysPhe: 2.099 ± 0.446
5.248LysGly: 5.248 ± 0.768
0.6LysHis: 0.6 ± 0.384
3.749LysIle: 3.749 ± 0.667
7.797LysLys: 7.797 ± 1.752
6.598LysLeu: 6.598 ± 1.64
2.699LysMet: 2.699 ± 0.64
4.948LysAsn: 4.948 ± 0.666
2.399LysPro: 2.399 ± 0.811
2.549LysGln: 2.549 ± 0.627
2.699LysArg: 2.699 ± 0.941
3.899LysSer: 3.899 ± 0.604
6.598LysThr: 6.598 ± 1.714
5.698LysVal: 5.698 ± 1.162
0.9LysTrp: 0.9 ± 0.339
2.999LysTyr: 2.999 ± 0.803
0.0LysXaa: 0.0 ± 0.0
Leu
4.199LeuAla: 4.199 ± 0.865
1.649LeuCys: 1.649 ± 0.626
4.498LeuAsp: 4.498 ± 0.842
6.298LeuGlu: 6.298 ± 0.839
3.449LeuPhe: 3.449 ± 1.004
1.2LeuGly: 1.2 ± 0.329
1.2LeuHis: 1.2 ± 0.501
3.449LeuIle: 3.449 ± 0.58
7.497LeuLys: 7.497 ± 1.022
5.248LeuLeu: 5.248 ± 1.494
2.849LeuMet: 2.849 ± 0.652
3.899LeuAsn: 3.899 ± 0.825
3.149LeuPro: 3.149 ± 0.704
3.449LeuGln: 3.449 ± 0.576
2.999LeuArg: 2.999 ± 0.742
7.048LeuSer: 7.048 ± 1.296
5.698LeuThr: 5.698 ± 0.994
1.649LeuVal: 1.649 ± 0.419
1.2LeuTrp: 1.2 ± 0.5
2.249LeuTyr: 2.249 ± 0.483
0.0LeuXaa: 0.0 ± 0.0
Met
2.549MetAla: 2.549 ± 0.652
0.15MetCys: 0.15 ± 0.147
1.649MetAsp: 1.649 ± 0.725
2.099MetGlu: 2.099 ± 0.654
1.499MetPhe: 1.499 ± 0.617
1.649MetGly: 1.649 ± 0.641
0.3MetHis: 0.3 ± 0.222
1.35MetIle: 1.35 ± 0.423
2.999MetLys: 2.999 ± 1.029
2.549MetLeu: 2.549 ± 0.78
0.3MetMet: 0.3 ± 0.188
2.249MetAsn: 2.249 ± 0.552
0.6MetPro: 0.6 ± 0.29
0.75MetGln: 0.75 ± 0.254
1.499MetArg: 1.499 ± 0.552
2.099MetSer: 2.099 ± 0.58
2.249MetThr: 2.249 ± 0.51
1.499MetVal: 1.499 ± 0.34
0.6MetTrp: 0.6 ± 0.328
1.35MetTyr: 1.35 ± 0.578
0.0MetXaa: 0.0 ± 0.0
Asn
3.299AsnAla: 3.299 ± 1.079
0.45AsnCys: 0.45 ± 0.301
2.099AsnAsp: 2.099 ± 0.67
3.299AsnGlu: 3.299 ± 0.661
1.949AsnPhe: 1.949 ± 0.663
4.798AsnGly: 4.798 ± 0.937
0.9AsnHis: 0.9 ± 0.386
2.549AsnIle: 2.549 ± 0.521
2.999AsnLys: 2.999 ± 0.449
4.199AsnLeu: 4.199 ± 0.863
0.9AsnMet: 0.9 ± 0.432
2.549AsnAsn: 2.549 ± 0.675
1.35AsnPro: 1.35 ± 0.464
1.05AsnGln: 1.05 ± 0.365
2.399AsnArg: 2.399 ± 0.555
3.449AsnSer: 3.449 ± 0.7
3.149AsnThr: 3.149 ± 0.579
4.498AsnVal: 4.498 ± 0.767
0.6AsnTrp: 0.6 ± 0.246
1.499AsnTyr: 1.499 ± 0.343
0.0AsnXaa: 0.0 ± 0.0
Pro
1.499ProAla: 1.499 ± 0.582
0.75ProCys: 0.75 ± 0.414
2.699ProAsp: 2.699 ± 0.853
3.299ProGlu: 3.299 ± 1.137
1.35ProPhe: 1.35 ± 0.655
0.0ProGly: 0.0 ± 0.0
1.05ProHis: 1.05 ± 0.337
1.949ProIle: 1.949 ± 0.45
0.75ProLys: 0.75 ± 0.32
2.699ProLeu: 2.699 ± 0.767
0.6ProMet: 0.6 ± 0.281
1.949ProAsn: 1.949 ± 0.626
2.699ProPro: 2.699 ± 0.975
1.2ProGln: 1.2 ± 0.407
1.05ProArg: 1.05 ± 0.479
1.949ProSer: 1.949 ± 0.581
2.249ProThr: 2.249 ± 0.677
2.699ProVal: 2.699 ± 0.676
0.15ProTrp: 0.15 ± 0.171
1.2ProTyr: 1.2 ± 0.494
0.0ProXaa: 0.0 ± 0.0
Gln
1.649GlnAla: 1.649 ± 0.502
0.45GlnCys: 0.45 ± 0.27
2.549GlnAsp: 2.549 ± 0.821
3.449GlnGlu: 3.449 ± 0.661
1.799GlnPhe: 1.799 ± 0.481
2.099GlnGly: 2.099 ± 0.496
0.15GlnHis: 0.15 ± 0.163
1.499GlnIle: 1.499 ± 0.593
2.699GlnLys: 2.699 ± 0.694
1.649GlnLeu: 1.649 ± 0.501
1.649GlnMet: 1.649 ± 0.513
1.499GlnAsn: 1.499 ± 0.483
1.499GlnPro: 1.499 ± 0.664
2.549GlnGln: 2.549 ± 0.674
2.099GlnArg: 2.099 ± 0.782
2.699GlnSer: 2.699 ± 0.567
1.949GlnThr: 1.949 ± 0.815
1.799GlnVal: 1.799 ± 0.498
0.3GlnTrp: 0.3 ± 0.177
1.649GlnTyr: 1.649 ± 0.645
0.0GlnXaa: 0.0 ± 0.0
Arg
1.499ArgAla: 1.499 ± 0.618
1.499ArgCys: 1.499 ± 0.604
1.949ArgAsp: 1.949 ± 0.608
3.299ArgGlu: 3.299 ± 1.072
2.099ArgPhe: 2.099 ± 0.515
2.849ArgGly: 2.849 ± 0.902
1.35ArgHis: 1.35 ± 0.706
2.099ArgIle: 2.099 ± 0.523
3.299ArgLys: 3.299 ± 0.966
3.299ArgLeu: 3.299 ± 0.907
2.099ArgMet: 2.099 ± 0.898
2.249ArgAsn: 2.249 ± 0.788
1.649ArgPro: 1.649 ± 0.6
0.6ArgGln: 0.6 ± 0.32
2.249ArgArg: 2.249 ± 0.593
2.699ArgSer: 2.699 ± 0.799
2.549ArgThr: 2.549 ± 0.626
1.649ArgVal: 1.649 ± 0.71
1.2ArgTrp: 1.2 ± 0.511
2.849ArgTyr: 2.849 ± 0.84
0.0ArgXaa: 0.0 ± 0.0
Ser
3.599SerAla: 3.599 ± 1.139
2.399SerCys: 2.399 ± 0.801
3.149SerAsp: 3.149 ± 0.707
3.899SerGlu: 3.899 ± 1.043
3.599SerPhe: 3.599 ± 1.094
6.748SerGly: 6.748 ± 1.151
1.649SerHis: 1.649 ± 0.57
4.648SerIle: 4.648 ± 0.722
4.199SerLys: 4.199 ± 0.704
5.848SerLeu: 5.848 ± 1.12
2.549SerMet: 2.549 ± 0.504
2.399SerAsn: 2.399 ± 0.81
1.649SerPro: 1.649 ± 0.55
2.249SerGln: 2.249 ± 0.548
1.649SerArg: 1.649 ± 0.461
4.199SerSer: 4.199 ± 1.068
4.648SerThr: 4.648 ± 1.895
5.098SerVal: 5.098 ± 0.934
0.45SerTrp: 0.45 ± 0.296
2.099SerTyr: 2.099 ± 0.43
0.0SerXaa: 0.0 ± 0.0
Thr
8.097ThrAla: 8.097 ± 2.501
1.499ThrCys: 1.499 ± 0.542
4.798ThrAsp: 4.798 ± 0.824
4.498ThrGlu: 4.498 ± 0.849
2.249ThrPhe: 2.249 ± 0.604
6.598ThrGly: 6.598 ± 1.495
0.3ThrHis: 0.3 ± 0.215
4.498ThrIle: 4.498 ± 1.039
4.948ThrLys: 4.948 ± 1.559
2.549ThrLeu: 2.549 ± 0.609
1.499ThrMet: 1.499 ± 0.422
2.999ThrAsn: 2.999 ± 1.049
2.549ThrPro: 2.549 ± 0.766
2.849ThrGln: 2.849 ± 0.609
3.149ThrArg: 3.149 ± 0.595
4.798ThrSer: 4.798 ± 0.953
4.498ThrThr: 4.498 ± 1.201
4.199ThrVal: 4.199 ± 0.852
0.45ThrTrp: 0.45 ± 0.28
1.649ThrTyr: 1.649 ± 0.5
0.0ThrXaa: 0.0 ± 0.0
Val
4.948ValAla: 4.948 ± 0.766
0.75ValCys: 0.75 ± 0.386
3.899ValAsp: 3.899 ± 0.702
4.648ValGlu: 4.648 ± 1.187
2.699ValPhe: 2.699 ± 0.64
3.449ValGly: 3.449 ± 0.747
0.9ValHis: 0.9 ± 0.379
4.498ValIle: 4.498 ± 0.955
5.248ValLys: 5.248 ± 0.982
4.199ValLeu: 4.199 ± 1.01
1.649ValMet: 1.649 ± 0.357
2.699ValAsn: 2.699 ± 0.507
2.699ValPro: 2.699 ± 0.611
1.649ValGln: 1.649 ± 0.548
2.549ValArg: 2.549 ± 0.76
4.498ValSer: 4.498 ± 0.862
3.599ValThr: 3.599 ± 0.883
3.749ValVal: 3.749 ± 0.662
0.15ValTrp: 0.15 ± 0.174
2.699ValTyr: 2.699 ± 0.897
0.0ValXaa: 0.0 ± 0.0
Trp
0.0TrpAla: 0.0 ± 0.0
0.15TrpCys: 0.15 ± 0.137
0.75TrpAsp: 0.75 ± 0.302
1.05TrpGlu: 1.05 ± 0.396
1.05TrpPhe: 1.05 ± 0.462
0.6TrpGly: 0.6 ± 0.288
0.3TrpHis: 0.3 ± 0.216
0.45TrpIle: 0.45 ± 0.242
1.649TrpLys: 1.649 ± 0.443
1.05TrpLeu: 1.05 ± 0.491
0.3TrpMet: 0.3 ± 0.205
0.6TrpAsn: 0.6 ± 0.282
0.0TrpPro: 0.0 ± 0.0
0.3TrpGln: 0.3 ± 0.226
0.45TrpArg: 0.45 ± 0.34
0.9TrpSer: 0.9 ± 0.498
0.3TrpThr: 0.3 ± 0.212
0.3TrpVal: 0.3 ± 0.234
0.0TrpTrp: 0.0 ± 0.0
1.2TrpTyr: 1.2 ± 0.308
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.35TyrAla: 1.35 ± 0.369
0.75TyrCys: 0.75 ± 0.358
3.149TyrAsp: 3.149 ± 0.846
3.449TyrGlu: 3.449 ± 0.805
1.2TyrPhe: 1.2 ± 0.633
1.649TyrGly: 1.649 ± 0.511
0.45TyrHis: 0.45 ± 0.315
2.699TyrIle: 2.699 ± 0.721
3.599TyrLys: 3.599 ± 0.704
1.949TyrLeu: 1.949 ± 0.711
1.05TyrMet: 1.05 ± 0.5
2.849TyrAsn: 2.849 ± 0.571
1.649TyrPro: 1.649 ± 0.703
1.05TyrGln: 1.05 ± 0.313
1.649TyrArg: 1.649 ± 0.59
1.35TyrSer: 1.35 ± 0.35
2.849TyrThr: 2.849 ± 0.583
3.599TyrVal: 3.599 ± 0.898
0.6TyrTrp: 0.6 ± 0.377
1.499TyrTyr: 1.499 ± 0.507
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 29 proteins (6670 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski