Fowl adenovirus
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 34 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P8NQN0|A0A5P8NQN0_9ADEN 22k protein OS=Fowl adenovirus OX=1354736 PE=4 SV=1
MM1 pKa = 7.52 AKK3 pKa = 8.76 STPFTFSMGQHH14 pKa = 6.21 SSRR17 pKa = 11.84 KK18 pKa = 9.39 RR19 pKa = 11.84 PADD22 pKa = 3.44 SEE24 pKa = 4.19 NTQNASKK31 pKa = 10.45 VAKK34 pKa = 8.38 TQTSATRR41 pKa = 11.84 AGVDD45 pKa = 3.89 GNDD48 pKa = 4.54 DD49 pKa = 4.1 LNLVYY54 pKa = 9.94 PFWLQNSTSGGGGGGSGGNPSLNPPFIDD82 pKa = 3.73 PNGPLYY88 pKa = 10.31 VQNSLLYY95 pKa = 10.35 VKK97 pKa = 7.76 TTAPIEE103 pKa = 4.16 VEE105 pKa = 4.18 NKK107 pKa = 10.27 SLALAYY113 pKa = 10.4 NSSLDD118 pKa = 3.5 VDD120 pKa = 4.2 AQNQLQVKK128 pKa = 9.2 VDD130 pKa = 3.45 AEE132 pKa = 4.46 GPIRR136 pKa = 11.84 ISPDD140 pKa = 3.08 GLDD143 pKa = 3.18 IAVDD147 pKa = 4.11 PSTLEE152 pKa = 4.49 VDD154 pKa = 4.89 DD155 pKa = 4.35 EE156 pKa = 4.52 WEE158 pKa = 4.23 LTVKK162 pKa = 10.5 LDD164 pKa = 3.59 PAGPISSSSAGINIRR179 pKa = 11.84 VDD181 pKa = 3.33 DD182 pKa = 4.2 TLLIEE187 pKa = 6.18 DD188 pKa = 5.61 DD189 pKa = 4.27 DD190 pKa = 4.23 TTQVKK195 pKa = 9.92 EE196 pKa = 4.4 LGVHH200 pKa = 6.68 LNPNGPITADD210 pKa = 3.16 QDD212 pKa = 4.14 GLDD215 pKa = 4.68 LEE217 pKa = 5.14 VDD219 pKa = 3.83 PQTLTVTTSGATGGVLGVLLKK240 pKa = 10.67 PSGGLQTSIQGIGVAVADD258 pKa = 4.18 TLTISSNNTVEE269 pKa = 4.99 VKK271 pKa = 9.88 TDD273 pKa = 3.71 PNGSIGSSSNGIAVVTDD290 pKa = 3.45 PAGPLTTSSNGLSLKK305 pKa = 8.58 LTPNGSIQSSSTGLSVQTDD324 pKa = 3.58 PAGPITSGANGLSLSYY340 pKa = 9.5 DD341 pKa = 3.29 TSDD344 pKa = 3.48 FTVSQGMLSIIRR356 pKa = 11.84 NPSTYY361 pKa = 9.77 PDD363 pKa = 3.91 AYY365 pKa = 10.87 LEE367 pKa = 4.61 SGTNLLNNYY376 pKa = 7.05 TAYY379 pKa = 10.66 AEE381 pKa = 4.05 NSSNYY386 pKa = 9.63 KK387 pKa = 9.97 FNCAYY392 pKa = 10.3 FLQSWYY398 pKa = 11.27 SNGLVTSSLYY408 pKa = 11.07 LKK410 pKa = 10.39 INRR413 pKa = 11.84 DD414 pKa = 3.68 NLTSLPSGQLSEE426 pKa = 3.98 NAKK429 pKa = 10.55 YY430 pKa = 8.24 FTFWVPTYY438 pKa = 11.17 EE439 pKa = 5.04 SMNLSNVATPTITPSSVPWGAFLPAQNCTSNPAFKK474 pKa = 10.69 YY475 pKa = 10.75 YY476 pKa = 9.51 LTQPPSIYY484 pKa = 10.32 FEE486 pKa = 4.53 PEE488 pKa = 3.66 SGSVQTFQPVLTGDD502 pKa = 3.49 WDD504 pKa = 4.24 TNTYY508 pKa = 10.85 NPGTVQVCILPQTVVGGQSTFVNMTCYY535 pKa = 10.52 NFRR538 pKa = 11.84 CQNPGIFKK546 pKa = 10.24 VAASSGTFTIGPIFYY561 pKa = 10.31 SCPTNKK567 pKa = 8.58 LTQPP571 pKa = 3.58
Molecular weight: 60.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.903
IPC2_protein 4.164
IPC_protein 4.164
Toseland 3.948
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.075
Rodwell 3.986
Grimsley 3.859
Solomon 4.139
Lehninger 4.101
Nozaki 4.253
DTASelect 4.507
Thurlkill 3.986
EMBOSS 4.088
Sillero 4.279
Patrickios 1.977
IPC_peptide 4.139
IPC2_peptide 4.266
IPC2.peptide.svr19 4.167
Protein with the highest isoelectric point:
>tr|A0A5P8NQL4|A0A5P8NQL4_9ADEN PTP protein OS=Fowl adenovirus OX=1354736 PE=4 SV=1
MM1 pKa = 7.68 SILISPNDD9 pKa = 3.25 NRR11 pKa = 11.84 GWGMRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 SSMRR24 pKa = 11.84 GVGIRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 LTLRR36 pKa = 11.84 TLLGLGTSSRR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 SGGRR52 pKa = 11.84 SSRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 SRR59 pKa = 11.84 PASTTTRR66 pKa = 11.84 VMLVRR71 pKa = 11.84 TSRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 3.32
Molecular weight: 9.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.296
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.258
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
33
1
34
11899
76
1305
350.0
39.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.841 ± 0.442
1.79 ± 0.26
5.572 ± 0.249
5.95 ± 0.52
4.152 ± 0.269
6.429 ± 0.316
2.378 ± 0.278
3.958 ± 0.174
3.706 ± 0.314
8.673 ± 0.334
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.395 ± 0.187
4.379 ± 0.487
6.454 ± 0.246
3.664 ± 0.239
7.37 ± 0.54
7.127 ± 0.424
6.043 ± 0.383
6.656 ± 0.277
1.361 ± 0.109
4.101 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here