Vibrio phage vB_VhaP_VH-5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Beijerinckvirinae; unclassified Beijerinckvirinae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WA29|A0A5Q2WA29_9CAUD Capsid protein OS=Vibrio phage vB_VhaP_VH-5 OX=2660694 PE=4 SV=1
MM1 pKa = 7.38HH2 pKa = 7.11FSEE5 pKa = 6.28DD6 pKa = 3.68EE7 pKa = 3.96LHH9 pKa = 7.34LEE11 pKa = 4.27VQNTLQVLRR20 pKa = 11.84GLDD23 pKa = 3.9YY24 pKa = 10.84QPHH27 pKa = 7.03LIGGVLRR34 pKa = 11.84VAALGGTTADD44 pKa = 3.03VDD46 pKa = 3.36IALIEE51 pKa = 4.84DD52 pKa = 3.79STDD55 pKa = 3.49QLVLDD60 pKa = 4.47VLLKK64 pKa = 10.75GLGYY68 pKa = 10.63NLQHH72 pKa = 5.99VQDD75 pKa = 3.69SKK77 pKa = 11.63YY78 pKa = 11.14ANEE81 pKa = 4.42TNGFLADD88 pKa = 3.77YY89 pKa = 10.26RR90 pKa = 11.84KK91 pKa = 10.62GDD93 pKa = 3.6INIILYY99 pKa = 10.55SSEE102 pKa = 3.78VYY104 pKa = 10.84NNVRR108 pKa = 11.84EE109 pKa = 4.28LVSSFDD115 pKa = 3.73LSINKK120 pKa = 9.78YY121 pKa = 9.89YY122 pKa = 10.74LDD124 pKa = 4.0SDD126 pKa = 4.3GVLCNDD132 pKa = 3.55HH133 pKa = 6.98FDD135 pKa = 4.06GYY137 pKa = 9.38VVKK140 pKa = 9.81CTEE143 pKa = 4.42SSNHH147 pKa = 3.94THH149 pKa = 5.6NLEE152 pKa = 4.45RR153 pKa = 11.84ITRR156 pKa = 11.84FKK158 pKa = 10.78TEE160 pKa = 4.14YY161 pKa = 9.97PDD163 pKa = 5.58LDD165 pKa = 3.3WTQPQSVLDD174 pKa = 3.89KK175 pKa = 10.8EE176 pKa = 4.76SGFEE180 pKa = 4.07VFSS183 pKa = 3.89

Molecular weight: 20.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W9X0|A0A5Q2W9X0_9CAUD Metallo-dependent phosphoesterase OS=Vibrio phage vB_VhaP_VH-5 OX=2660694 PE=4 SV=1
MM1 pKa = 7.57SNRR4 pKa = 11.84KK5 pKa = 8.53NVLKK9 pKa = 10.46RR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.44VKK14 pKa = 9.94AAPKK18 pKa = 9.56PIKK21 pKa = 9.96QNPPINHH28 pKa = 6.29KK29 pKa = 8.47TDD31 pKa = 3.23QVVRR35 pKa = 11.84SIYY38 pKa = 10.21FGYY41 pKa = 10.86AGTDD45 pKa = 3.28KK46 pKa = 10.59PLVSSPVKK54 pKa = 10.71GNALMFFEE62 pKa = 4.75PEE64 pKa = 4.09SYY66 pKa = 10.7VVVGIADD73 pKa = 3.22ARR75 pKa = 11.84GYY77 pKa = 10.88RR78 pKa = 11.84FTPATNSALISLGYY92 pKa = 10.44HH93 pKa = 5.33PTAVLTWLSQLHH105 pKa = 6.56LNGAPVVQAQGDD117 pKa = 3.98EE118 pKa = 4.33LVAVHH123 pKa = 6.8AAVVQCARR131 pKa = 11.84DD132 pKa = 3.44VVMNYY137 pKa = 9.07EE138 pKa = 4.0KK139 pKa = 11.07LGMVKK144 pKa = 10.32GKK146 pKa = 9.43PRR148 pKa = 11.84VLSKK152 pKa = 10.75PILEE156 pKa = 4.6HH157 pKa = 5.81YY158 pKa = 9.61KK159 pKa = 10.8AVGKK163 pKa = 10.12HH164 pKa = 5.44PLFAA168 pKa = 6.23

Molecular weight: 18.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

11317

59

1136

353.7

39.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.492 ± 0.489

1.087 ± 0.188

6.062 ± 0.229

6.221 ± 0.337

3.34 ± 0.18

6.998 ± 0.26

2.13 ± 0.217

4.374 ± 0.267

5.699 ± 0.301

8.907 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.176

4.613 ± 0.325

3.72 ± 0.226

4.984 ± 0.361

5.284 ± 0.326

6.079 ± 0.272

6.875 ± 0.383

7.511 ± 0.405

1.113 ± 0.123

4.127 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski