Spissistilus festinus reovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; unclassified Reoviridae

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|H6BDY2|H6BDY2_9REOV p9 OS=Spissistilus festinus reovirus OX=1004049 GN=P9 PE=4 SV=1
MM1 pKa = 8.05DD2 pKa = 5.73LGEE5 pKa = 4.45NLYY8 pKa = 10.54SLHH11 pKa = 7.2AIIRR15 pKa = 11.84NVYY18 pKa = 10.05EE19 pKa = 4.02YY20 pKa = 9.64TQSNTLEE27 pKa = 4.09NAQAGQITEE36 pKa = 4.19EE37 pKa = 4.35FQAGPITHH45 pKa = 7.03HH46 pKa = 6.94FDD48 pKa = 4.82FITVSARR55 pKa = 11.84LGLRR59 pKa = 11.84LDD61 pKa = 4.37FAYY64 pKa = 10.07QQYY67 pKa = 9.58TIEE70 pKa = 4.45SNVAKK75 pKa = 10.19WNADD79 pKa = 2.91HH80 pKa = 7.21RR81 pKa = 11.84AHH83 pKa = 6.23FHH85 pKa = 6.16SADD88 pKa = 3.3AVLRR92 pKa = 11.84ALSSLAVHH100 pKa = 6.4TEE102 pKa = 4.37LYY104 pKa = 10.2APSYY108 pKa = 10.77RR109 pKa = 11.84FATSDD114 pKa = 3.67DD115 pKa = 3.39ATLAHH120 pKa = 6.65IYY122 pKa = 9.55EE123 pKa = 4.32WASDD127 pKa = 3.6SSVSRR132 pKa = 11.84FAVLDD137 pKa = 3.26ISGIRR142 pKa = 11.84FKK144 pKa = 11.44SLVSDD149 pKa = 4.59LNSEE153 pKa = 4.34HH154 pKa = 6.33GRR156 pKa = 11.84SSSLPEE162 pKa = 3.74EE163 pKa = 4.7PGPSFTVNNPPALLPFGEE181 pKa = 4.38SEE183 pKa = 4.17QLNEE187 pKa = 3.93VDD189 pKa = 3.67LHH191 pKa = 7.01GSVADD196 pKa = 3.63TRR198 pKa = 11.84AYY200 pKa = 10.35RR201 pKa = 11.84FHH203 pKa = 7.46APRR206 pKa = 11.84LPIPISVVQEE216 pKa = 3.55EE217 pKa = 4.67STALFSYY224 pKa = 10.04KK225 pKa = 10.05YY226 pKa = 10.09RR227 pKa = 11.84PNEE230 pKa = 3.76GWWRR234 pKa = 11.84DD235 pKa = 3.81SYY237 pKa = 10.94PNSYY241 pKa = 9.42VFQYY245 pKa = 10.78YY246 pKa = 10.65GEE248 pKa = 4.38TTPYY252 pKa = 9.86TADD255 pKa = 3.32QIVARR260 pKa = 11.84FTPLQLPEE268 pKa = 4.48VVSPPIPIIALPYY281 pKa = 10.04ASLVTDD287 pKa = 4.19ISSSPSFDD295 pKa = 5.37LNLLLQNGLWLASINYY311 pKa = 7.65EE312 pKa = 4.24ALKK315 pKa = 10.72LLNTLACSYY324 pKa = 10.21LAVYY328 pKa = 7.65GTLAMQEE335 pKa = 4.07RR336 pKa = 11.84VPPIFFTVGLTPEE349 pKa = 4.04VTDD352 pKa = 3.69DD353 pKa = 3.51FLYY356 pKa = 9.42VTRR359 pKa = 11.84PRR361 pKa = 11.84KK362 pKa = 9.55GGIYY366 pKa = 9.88LEE368 pKa = 4.4HH369 pKa = 6.95SAMSSLFDD377 pKa = 3.71KK378 pKa = 10.57VASVIGGYY386 pKa = 8.39ILKK389 pKa = 10.45VPIHH393 pKa = 6.75TYY395 pKa = 9.13AQCSSRR401 pKa = 11.84MWVFANGLPNGQLQRR416 pKa = 11.84INYY419 pKa = 7.05QWPLVDD425 pKa = 3.97ISSTLGLKK433 pKa = 10.28NGTRR437 pKa = 11.84FGLAITFRR445 pKa = 11.84DD446 pKa = 3.4MLEE449 pKa = 4.03SVKK452 pKa = 10.11TYY454 pKa = 11.24NLVPLLLEE462 pKa = 5.1LSYY465 pKa = 11.07QQDD468 pKa = 3.34NDD470 pKa = 4.01LTPVIPTAEE479 pKa = 3.93YY480 pKa = 10.25LIWYY484 pKa = 8.08WMFFFPFATEE494 pKa = 3.84YY495 pKa = 10.73NVNFRR500 pKa = 11.84EE501 pKa = 4.23PASVTVGTFPITGPPGARR519 pKa = 11.84FPATFDD525 pKa = 3.4YY526 pKa = 10.95RR527 pKa = 11.84STQVADD533 pKa = 3.36YY534 pKa = 9.92QRR536 pKa = 11.84YY537 pKa = 7.87FIEE540 pKa = 4.21VFFVSARR547 pKa = 11.84RR548 pKa = 11.84INLCC552 pKa = 3.82

Molecular weight:
62.31 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|H6BDX6|H6BDX6_9REOV Major core capsid OS=Spissistilus festinus reovirus OX=1004049 GN=P3 PE=4 SV=1
MM1 pKa = 7.4SEE3 pKa = 3.92AVEE6 pKa = 4.45TFLKK10 pKa = 10.19TLTIPHH16 pKa = 7.56DD17 pKa = 4.2DD18 pKa = 3.41VTILQEE24 pKa = 3.98VSSICSTPNSIFEE37 pKa = 4.33YY38 pKa = 10.16IKK40 pKa = 10.53RR41 pKa = 11.84DD42 pKa = 3.98YY43 pKa = 11.55NPLTPGKK50 pKa = 9.67YY51 pKa = 9.35HH52 pKa = 6.82GEE54 pKa = 4.22SASGQSIQVAFTIPKK69 pKa = 8.97EE70 pKa = 4.16FHH72 pKa = 6.78SPPDD76 pKa = 3.44VNPNSFEE83 pKa = 3.92YY84 pKa = 10.87SRR86 pKa = 11.84TSFGLKK92 pKa = 9.72YY93 pKa = 10.24SLSGVEE99 pKa = 3.88YY100 pKa = 9.68EE101 pKa = 4.38YY102 pKa = 11.4NYY104 pKa = 10.99LPTFNVSTFPTVPALNLADD123 pKa = 3.86SGNVVLNLIKK133 pKa = 10.45EE134 pKa = 4.39LLSSSQNQSRR144 pKa = 11.84VAGIWKK150 pKa = 8.13QTAAMVALLRR160 pKa = 11.84AYY162 pKa = 10.11RR163 pKa = 11.84DD164 pKa = 3.64KK165 pKa = 11.59NHH167 pKa = 5.55VLLRR171 pKa = 11.84YY172 pKa = 8.7FHH174 pKa = 7.0LLVGSYY180 pKa = 8.9VAPPFYY186 pKa = 10.42EE187 pKa = 4.16KK188 pKa = 10.33EE189 pKa = 4.17GKK191 pKa = 9.55IVWEE195 pKa = 4.35KK196 pKa = 9.66TRR198 pKa = 11.84QPVLSVLPLLLHH210 pKa = 6.47GAGHH214 pKa = 7.07LAIMHH219 pKa = 6.11FTNVVTEE226 pKa = 4.2VDD228 pKa = 4.44CIMCIQHH235 pKa = 6.81WMDD238 pKa = 4.42CLPGGFQDD246 pKa = 5.06RR247 pKa = 11.84KK248 pKa = 10.67LKK250 pKa = 9.8TKK252 pKa = 10.3SHH254 pKa = 6.63LSNWLPYY261 pKa = 9.76SLHH264 pKa = 6.83RR265 pKa = 11.84LPPQKK270 pKa = 9.97IAMWSQTGCLMGTTTRR286 pKa = 11.84DD287 pKa = 3.05TTEE290 pKa = 4.03PDD292 pKa = 2.61IVMQEE297 pKa = 3.86EE298 pKa = 4.13AGRR301 pKa = 11.84NHH303 pKa = 6.7AAKK306 pKa = 10.44LNLSSEE312 pKa = 4.15VEE314 pKa = 3.97RR315 pKa = 11.84YY316 pKa = 9.06VRR318 pKa = 11.84HH319 pKa = 5.67LQSASSLDD327 pKa = 3.67SCIAGYY333 pKa = 10.3LVGRR337 pKa = 11.84VMSNDD342 pKa = 2.68GTYY345 pKa = 9.98FASLPEE351 pKa = 3.84ITIDD355 pKa = 3.42KK356 pKa = 10.56AVLPRR361 pKa = 11.84VEE363 pKa = 3.99HH364 pKa = 5.66HH365 pKa = 7.16VIRR368 pKa = 11.84TMRR371 pKa = 11.84PRR373 pKa = 11.84SHH375 pKa = 6.55LTNSQVLYY383 pKa = 10.55YY384 pKa = 10.24DD385 pKa = 3.39QTSVPEE391 pKa = 4.09FLPGYY396 pKa = 9.43NYY398 pKa = 10.4LRR400 pKa = 11.84RR401 pKa = 11.84AFPQWRR407 pKa = 11.84ANSAVLLSNLNYY419 pKa = 10.35EE420 pKa = 4.97IIPFLTTSSAGQLPEE435 pKa = 4.22GGQRR439 pKa = 11.84VQHH442 pKa = 6.08ADD444 pKa = 2.75KK445 pKa = 10.81RR446 pKa = 11.84LRR448 pKa = 11.84AASKK452 pKa = 10.0KK453 pKa = 10.18RR454 pKa = 11.84VIAFIDD460 pKa = 3.49EE461 pKa = 4.37RR462 pKa = 11.84EE463 pKa = 4.26DD464 pKa = 3.56YY465 pKa = 11.5LNLEE469 pKa = 4.39RR470 pKa = 11.84FQARR474 pKa = 11.84LVDD477 pKa = 3.54VVVAVEE483 pKa = 4.15RR484 pKa = 11.84TQILRR489 pKa = 11.84RR490 pKa = 11.84ARR492 pKa = 11.84AVAGVNNPRR501 pKa = 11.84ILASLPVLRR510 pKa = 11.84ALEE513 pKa = 4.22EE514 pKa = 4.14LQHH517 pKa = 6.64LLPQASSGKK526 pKa = 9.91QKK528 pKa = 11.41GDD530 pKa = 3.42MSDD533 pKa = 3.66MLPLLYY539 pKa = 9.34YY540 pKa = 9.96TSSANVLCSSMDD552 pKa = 3.15VSGFDD557 pKa = 3.79ASVQLVSQMQTLSMTSEE574 pKa = 3.95ALADD578 pKa = 4.25VPCHH582 pKa = 7.08KK583 pKa = 10.07YY584 pKa = 9.91LCYY587 pKa = 9.83RR588 pKa = 11.84AHH590 pKa = 5.9QHH592 pKa = 5.93TDD594 pKa = 3.59PITQEE599 pKa = 3.92TSIVSAFSYY608 pKa = 10.51LISDD612 pKa = 3.61IMTRR616 pKa = 11.84FQPQSTRR623 pKa = 11.84VKK625 pKa = 10.24GKK627 pKa = 9.46VIPYY631 pKa = 7.88VTTADD636 pKa = 3.47PTFPSGLGYY645 pKa = 7.11TTAHH649 pKa = 5.29HH650 pKa = 6.94TIRR653 pKa = 11.84LISAIFGDD661 pKa = 3.84VFRR664 pKa = 11.84DD665 pKa = 3.4LRR667 pKa = 11.84FGILSSIRR675 pKa = 11.84HH676 pKa = 5.24TAVQGDD682 pKa = 4.67DD683 pKa = 2.62ISMAFRR689 pKa = 11.84GSPQKK694 pKa = 10.27RR695 pKa = 11.84ARR697 pKa = 11.84DD698 pKa = 3.55LKK700 pKa = 10.54RR701 pKa = 11.84VQDD704 pKa = 4.15SIEE707 pKa = 3.9KK708 pKa = 10.65VGFKK712 pKa = 9.95TEE714 pKa = 4.18NEE716 pKa = 4.34SSVSTMEE723 pKa = 4.2FLQQRR728 pKa = 11.84AVLGRR733 pKa = 11.84PVLYY737 pKa = 9.62PDD739 pKa = 3.63RR740 pKa = 11.84VSLLTAEE747 pKa = 5.03KK748 pKa = 9.92PRR750 pKa = 11.84EE751 pKa = 4.02AKK753 pKa = 10.85GPVEE757 pKa = 4.78KK758 pKa = 10.19MSEE761 pKa = 4.2LLALAYY767 pKa = 10.28DD768 pKa = 4.27LGNRR772 pKa = 11.84VPNPHH777 pKa = 6.6MLLPILYY784 pKa = 10.07ALYY787 pKa = 10.09LFCAAKK793 pKa = 8.99VTLKK797 pKa = 10.48ISTGAARR804 pKa = 11.84KK805 pKa = 9.55LYY807 pKa = 9.7EE808 pKa = 4.42DD809 pKa = 3.74KK810 pKa = 11.26EE811 pKa = 4.36FIEE814 pKa = 5.28RR815 pKa = 11.84IFGRR819 pKa = 11.84GIIEE823 pKa = 4.04QDD825 pKa = 3.34PKK827 pKa = 11.31EE828 pKa = 4.3CTHH831 pKa = 6.53DD832 pKa = 3.27TFFFSFIAPLSYY844 pKa = 10.66AWVEE848 pKa = 4.12GGPMLPPPSYY858 pKa = 9.87RR859 pKa = 11.84RR860 pKa = 11.84KK861 pKa = 10.32DD862 pKa = 3.45GTYY865 pKa = 9.56SPVPNFYY872 pKa = 10.61SLRR875 pKa = 11.84GAYY878 pKa = 9.97AKK880 pKa = 9.91TFLFHH885 pKa = 7.58LSLTSNMWKK894 pKa = 10.23EE895 pKa = 3.79LMRR898 pKa = 11.84RR899 pKa = 11.84DD900 pKa = 3.56EE901 pKa = 4.39EE902 pKa = 4.36GAIIDD907 pKa = 4.05ASLLLDD913 pKa = 4.16YY914 pKa = 11.11EE915 pKa = 4.44LLDD918 pKa = 4.58DD919 pKa = 4.94RR920 pKa = 11.84NVLMGVHH927 pKa = 6.36LAEE930 pKa = 4.4TLSKK934 pKa = 10.49FKK936 pKa = 10.3TVSDD940 pKa = 3.53VDD942 pKa = 3.21IEE944 pKa = 4.37RR945 pKa = 11.84EE946 pKa = 3.97YY947 pKa = 10.54RR948 pKa = 11.84TALPQMAAALHH959 pKa = 6.95AYY961 pKa = 9.74QNLRR965 pKa = 11.84ANQRR969 pKa = 11.84SLDD972 pKa = 3.72AAAKK976 pKa = 9.21LHH978 pKa = 6.08AAGVTLPSAIIKK990 pKa = 10.28GLEE993 pKa = 3.84AEE995 pKa = 4.46TKK997 pKa = 10.21IKK999 pKa = 9.65GTLRR1003 pKa = 11.84SVASSPFEE1011 pKa = 4.27EE1012 pKa = 4.57IVRR1015 pKa = 11.84ADD1017 pKa = 3.63KK1018 pKa = 10.74LALMLLEE1025 pKa = 4.63SFRR1028 pKa = 11.84SYY1030 pKa = 10.94KK1031 pKa = 9.9KK1032 pKa = 9.44NRR1034 pKa = 11.84RR1035 pKa = 11.84LRR1037 pKa = 11.84VHH1039 pKa = 6.86PEE1041 pKa = 4.0DD1042 pKa = 4.19YY1043 pKa = 10.88SCLYY1047 pKa = 8.25YY1048 pKa = 10.86CEE1050 pKa = 4.38WEE1052 pKa = 4.19EE1053 pKa = 4.25GQLITPSTAYY1063 pKa = 9.92EE1064 pKa = 3.98VIYY1067 pKa = 10.63EE1068 pKa = 4.17NGVTLSPDD1076 pKa = 3.24ANKK1079 pKa = 10.82GSIASVLMAYY1089 pKa = 10.56LGTRR1093 pKa = 11.84SGFQPHH1099 pKa = 7.15GDD1101 pKa = 3.02PFMQKK1106 pKa = 7.4VQRR1109 pKa = 11.84AFKK1112 pKa = 10.68DD1113 pKa = 4.03FVRR1116 pKa = 11.84GDD1118 pKa = 3.66EE1119 pKa = 4.11VLEE1122 pKa = 4.63AGLKK1126 pKa = 8.73VLRR1129 pKa = 11.84HH1130 pKa = 5.35APEE1133 pKa = 5.47LIRR1136 pKa = 11.84EE1137 pKa = 4.52FSTAVGLSKK1146 pKa = 10.72AQEE1149 pKa = 3.9NRR1151 pKa = 11.84LIALAPTYY1159 pKa = 10.67AGSKK1163 pKa = 7.26YY1164 pKa = 7.7TYY1166 pKa = 10.12HH1167 pKa = 7.36RR1168 pKa = 11.84IIYY1171 pKa = 9.71SPRR1174 pKa = 11.84QYY1176 pKa = 11.46FMVNSDD1182 pKa = 3.73PYY1184 pKa = 11.11ALRR1187 pKa = 11.84SHH1189 pKa = 7.53ISCQHH1194 pKa = 4.83QGGPLLIALSCVQAQEE1210 pKa = 3.9WLSYY1214 pKa = 10.63NVPTSKK1220 pKa = 11.01GEE1222 pKa = 4.08RR1223 pKa = 11.84IKK1225 pKa = 11.14VYY1227 pKa = 10.48LPWRR1231 pKa = 11.84TLEE1234 pKa = 3.71RR1235 pKa = 11.84LGRR1238 pKa = 11.84LKK1240 pKa = 10.23QACC1243 pKa = 3.63

Molecular weight:
140.0 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

8024

334

1310

802.4

89.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.328 ± 0.199

1.309 ± 0.221

5.309 ± 0.389

4.723 ± 0.23

3.801 ± 0.257

5.77 ± 0.263

2.493 ± 0.234

5.82 ± 0.307

3.863 ± 0.294

10.057 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.168 ± 0.138

3.926 ± 0.239

5.733 ± 0.258

3.739 ± 0.237

5.558 ± 0.221

8.923 ± 0.434

6.381 ± 0.297

7.814 ± 0.373

1.346 ± 0.212

3.938 ± 0.243

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski