Spissistilus festinus reovirus
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H6BDY2|H6BDY2_9REOV p9 OS=Spissistilus festinus reovirus OX=1004049 GN=P9 PE=4 SV=1
MM1 pKa = 8.05 DD2 pKa = 5.73 LGEE5 pKa = 4.45 NLYY8 pKa = 10.54 SLHH11 pKa = 7.2 AIIRR15 pKa = 11.84 NVYY18 pKa = 10.05 EE19 pKa = 4.02 YY20 pKa = 9.64 TQSNTLEE27 pKa = 4.09 NAQAGQITEE36 pKa = 4.19 EE37 pKa = 4.35 FQAGPITHH45 pKa = 7.03 HH46 pKa = 6.94 FDD48 pKa = 4.82 FITVSARR55 pKa = 11.84 LGLRR59 pKa = 11.84 LDD61 pKa = 4.37 FAYY64 pKa = 10.07 QQYY67 pKa = 9.58 TIEE70 pKa = 4.45 SNVAKK75 pKa = 10.19 WNADD79 pKa = 2.91 HH80 pKa = 7.21 RR81 pKa = 11.84 AHH83 pKa = 6.23 FHH85 pKa = 6.16 SADD88 pKa = 3.3 AVLRR92 pKa = 11.84 ALSSLAVHH100 pKa = 6.4 TEE102 pKa = 4.37 LYY104 pKa = 10.2 APSYY108 pKa = 10.77 RR109 pKa = 11.84 FATSDD114 pKa = 3.67 DD115 pKa = 3.39 ATLAHH120 pKa = 6.65 IYY122 pKa = 9.55 EE123 pKa = 4.32 WASDD127 pKa = 3.6 SSVSRR132 pKa = 11.84 FAVLDD137 pKa = 3.26 ISGIRR142 pKa = 11.84 FKK144 pKa = 11.44 SLVSDD149 pKa = 4.59 LNSEE153 pKa = 4.34 HH154 pKa = 6.33 GRR156 pKa = 11.84 SSSLPEE162 pKa = 3.74 EE163 pKa = 4.7 PGPSFTVNNPPALLPFGEE181 pKa = 4.38 SEE183 pKa = 4.17 QLNEE187 pKa = 3.93 VDD189 pKa = 3.67 LHH191 pKa = 7.01 GSVADD196 pKa = 3.63 TRR198 pKa = 11.84 AYY200 pKa = 10.35 RR201 pKa = 11.84 FHH203 pKa = 7.46 APRR206 pKa = 11.84 LPIPISVVQEE216 pKa = 3.55 EE217 pKa = 4.67 STALFSYY224 pKa = 10.04 KK225 pKa = 10.05 YY226 pKa = 10.09 RR227 pKa = 11.84 PNEE230 pKa = 3.76 GWWRR234 pKa = 11.84 DD235 pKa = 3.81 SYY237 pKa = 10.94 PNSYY241 pKa = 9.42 VFQYY245 pKa = 10.78 YY246 pKa = 10.65 GEE248 pKa = 4.38 TTPYY252 pKa = 9.86 TADD255 pKa = 3.32 QIVARR260 pKa = 11.84 FTPLQLPEE268 pKa = 4.48 VVSPPIPIIALPYY281 pKa = 10.04 ASLVTDD287 pKa = 4.19 ISSSPSFDD295 pKa = 5.37 LNLLLQNGLWLASINYY311 pKa = 7.65 EE312 pKa = 4.24 ALKK315 pKa = 10.72 LLNTLACSYY324 pKa = 10.21 LAVYY328 pKa = 7.65 GTLAMQEE335 pKa = 4.07 RR336 pKa = 11.84 VPPIFFTVGLTPEE349 pKa = 4.04 VTDD352 pKa = 3.69 DD353 pKa = 3.51 FLYY356 pKa = 9.42 VTRR359 pKa = 11.84 PRR361 pKa = 11.84 KK362 pKa = 9.55 GGIYY366 pKa = 9.88 LEE368 pKa = 4.4 HH369 pKa = 6.95 SAMSSLFDD377 pKa = 3.71 KK378 pKa = 10.57 VASVIGGYY386 pKa = 8.39 ILKK389 pKa = 10.45 VPIHH393 pKa = 6.75 TYY395 pKa = 9.13 AQCSSRR401 pKa = 11.84 MWVFANGLPNGQLQRR416 pKa = 11.84 INYY419 pKa = 7.05 QWPLVDD425 pKa = 3.97 ISSTLGLKK433 pKa = 10.28 NGTRR437 pKa = 11.84 FGLAITFRR445 pKa = 11.84 DD446 pKa = 3.4 MLEE449 pKa = 4.03 SVKK452 pKa = 10.11 TYY454 pKa = 11.24 NLVPLLLEE462 pKa = 5.1 LSYY465 pKa = 11.07 QQDD468 pKa = 3.34 NDD470 pKa = 4.01 LTPVIPTAEE479 pKa = 3.93 YY480 pKa = 10.25 LIWYY484 pKa = 8.08 WMFFFPFATEE494 pKa = 3.84 YY495 pKa = 10.73 NVNFRR500 pKa = 11.84 EE501 pKa = 4.23 PASVTVGTFPITGPPGARR519 pKa = 11.84 FPATFDD525 pKa = 3.4 YY526 pKa = 10.95 RR527 pKa = 11.84 STQVADD533 pKa = 3.36 YY534 pKa = 9.92 QRR536 pKa = 11.84 YY537 pKa = 7.87 FIEE540 pKa = 4.21 VFFVSARR547 pKa = 11.84 RR548 pKa = 11.84 INLCC552 pKa = 3.82
Molecular weight: 62.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.914
IPC2_protein 5.092
IPC_protein 5.067
Toseland 5.092
ProMoST 5.245
Dawson 5.105
Bjellqvist 5.207
Wikipedia 4.991
Rodwell 5.029
Grimsley 5.041
Solomon 5.105
Lehninger 5.067
Nozaki 5.245
DTASelect 5.397
Thurlkill 5.105
EMBOSS 5.067
Sillero 5.321
Patrickios 2.791
IPC_peptide 5.105
IPC2_peptide 5.321
IPC2.peptide.svr19 5.201
Protein with the highest isoelectric point:
>tr|H6BDX6|H6BDX6_9REOV Major core capsid OS=Spissistilus festinus reovirus OX=1004049 GN=P3 PE=4 SV=1
MM1 pKa = 7.4 SEE3 pKa = 3.92 AVEE6 pKa = 4.45 TFLKK10 pKa = 10.19 TLTIPHH16 pKa = 7.56 DD17 pKa = 4.2 DD18 pKa = 3.41 VTILQEE24 pKa = 3.98 VSSICSTPNSIFEE37 pKa = 4.33 YY38 pKa = 10.16 IKK40 pKa = 10.53 RR41 pKa = 11.84 DD42 pKa = 3.98 YY43 pKa = 11.55 NPLTPGKK50 pKa = 9.67 YY51 pKa = 9.35 HH52 pKa = 6.82 GEE54 pKa = 4.22 SASGQSIQVAFTIPKK69 pKa = 8.97 EE70 pKa = 4.16 FHH72 pKa = 6.78 SPPDD76 pKa = 3.44 VNPNSFEE83 pKa = 3.92 YY84 pKa = 10.87 SRR86 pKa = 11.84 TSFGLKK92 pKa = 9.72 YY93 pKa = 10.24 SLSGVEE99 pKa = 3.88 YY100 pKa = 9.68 EE101 pKa = 4.38 YY102 pKa = 11.4 NYY104 pKa = 10.99 LPTFNVSTFPTVPALNLADD123 pKa = 3.86 SGNVVLNLIKK133 pKa = 10.45 EE134 pKa = 4.39 LLSSSQNQSRR144 pKa = 11.84 VAGIWKK150 pKa = 8.13 QTAAMVALLRR160 pKa = 11.84 AYY162 pKa = 10.11 RR163 pKa = 11.84 DD164 pKa = 3.64 KK165 pKa = 11.59 NHH167 pKa = 5.55 VLLRR171 pKa = 11.84 YY172 pKa = 8.7 FHH174 pKa = 7.0 LLVGSYY180 pKa = 8.9 VAPPFYY186 pKa = 10.42 EE187 pKa = 4.16 KK188 pKa = 10.33 EE189 pKa = 4.17 GKK191 pKa = 9.55 IVWEE195 pKa = 4.35 KK196 pKa = 9.66 TRR198 pKa = 11.84 QPVLSVLPLLLHH210 pKa = 6.47 GAGHH214 pKa = 7.07 LAIMHH219 pKa = 6.11 FTNVVTEE226 pKa = 4.2 VDD228 pKa = 4.44 CIMCIQHH235 pKa = 6.81 WMDD238 pKa = 4.42 CLPGGFQDD246 pKa = 5.06 RR247 pKa = 11.84 KK248 pKa = 10.67 LKK250 pKa = 9.8 TKK252 pKa = 10.3 SHH254 pKa = 6.63 LSNWLPYY261 pKa = 9.76 SLHH264 pKa = 6.83 RR265 pKa = 11.84 LPPQKK270 pKa = 9.97 IAMWSQTGCLMGTTTRR286 pKa = 11.84 DD287 pKa = 3.05 TTEE290 pKa = 4.03 PDD292 pKa = 2.61 IVMQEE297 pKa = 3.86 EE298 pKa = 4.13 AGRR301 pKa = 11.84 NHH303 pKa = 6.7 AAKK306 pKa = 10.44 LNLSSEE312 pKa = 4.15 VEE314 pKa = 3.97 RR315 pKa = 11.84 YY316 pKa = 9.06 VRR318 pKa = 11.84 HH319 pKa = 5.67 LQSASSLDD327 pKa = 3.67 SCIAGYY333 pKa = 10.3 LVGRR337 pKa = 11.84 VMSNDD342 pKa = 2.68 GTYY345 pKa = 9.98 FASLPEE351 pKa = 3.84 ITIDD355 pKa = 3.42 KK356 pKa = 10.56 AVLPRR361 pKa = 11.84 VEE363 pKa = 3.99 HH364 pKa = 5.66 HH365 pKa = 7.16 VIRR368 pKa = 11.84 TMRR371 pKa = 11.84 PRR373 pKa = 11.84 SHH375 pKa = 6.55 LTNSQVLYY383 pKa = 10.55 YY384 pKa = 10.24 DD385 pKa = 3.39 QTSVPEE391 pKa = 4.09 FLPGYY396 pKa = 9.43 NYY398 pKa = 10.4 LRR400 pKa = 11.84 RR401 pKa = 11.84 AFPQWRR407 pKa = 11.84 ANSAVLLSNLNYY419 pKa = 10.35 EE420 pKa = 4.97 IIPFLTTSSAGQLPEE435 pKa = 4.22 GGQRR439 pKa = 11.84 VQHH442 pKa = 6.08 ADD444 pKa = 2.75 KK445 pKa = 10.81 RR446 pKa = 11.84 LRR448 pKa = 11.84 AASKK452 pKa = 10.0 KK453 pKa = 10.18 RR454 pKa = 11.84 VIAFIDD460 pKa = 3.49 EE461 pKa = 4.37 RR462 pKa = 11.84 EE463 pKa = 4.26 DD464 pKa = 3.56 YY465 pKa = 11.5 LNLEE469 pKa = 4.39 RR470 pKa = 11.84 FQARR474 pKa = 11.84 LVDD477 pKa = 3.54 VVVAVEE483 pKa = 4.15 RR484 pKa = 11.84 TQILRR489 pKa = 11.84 RR490 pKa = 11.84 ARR492 pKa = 11.84 AVAGVNNPRR501 pKa = 11.84 ILASLPVLRR510 pKa = 11.84 ALEE513 pKa = 4.22 EE514 pKa = 4.14 LQHH517 pKa = 6.64 LLPQASSGKK526 pKa = 9.91 QKK528 pKa = 11.41 GDD530 pKa = 3.42 MSDD533 pKa = 3.66 MLPLLYY539 pKa = 9.34 YY540 pKa = 9.96 TSSANVLCSSMDD552 pKa = 3.15 VSGFDD557 pKa = 3.79 ASVQLVSQMQTLSMTSEE574 pKa = 3.95 ALADD578 pKa = 4.25 VPCHH582 pKa = 7.08 KK583 pKa = 10.07 YY584 pKa = 9.91 LCYY587 pKa = 9.83 RR588 pKa = 11.84 AHH590 pKa = 5.9 QHH592 pKa = 5.93 TDD594 pKa = 3.59 PITQEE599 pKa = 3.92 TSIVSAFSYY608 pKa = 10.51 LISDD612 pKa = 3.61 IMTRR616 pKa = 11.84 FQPQSTRR623 pKa = 11.84 VKK625 pKa = 10.24 GKK627 pKa = 9.46 VIPYY631 pKa = 7.88 VTTADD636 pKa = 3.47 PTFPSGLGYY645 pKa = 7.11 TTAHH649 pKa = 5.29 HH650 pKa = 6.94 TIRR653 pKa = 11.84 LISAIFGDD661 pKa = 3.84 VFRR664 pKa = 11.84 DD665 pKa = 3.4 LRR667 pKa = 11.84 FGILSSIRR675 pKa = 11.84 HH676 pKa = 5.24 TAVQGDD682 pKa = 4.67 DD683 pKa = 2.62 ISMAFRR689 pKa = 11.84 GSPQKK694 pKa = 10.27 RR695 pKa = 11.84 ARR697 pKa = 11.84 DD698 pKa = 3.55 LKK700 pKa = 10.54 RR701 pKa = 11.84 VQDD704 pKa = 4.15 SIEE707 pKa = 3.9 KK708 pKa = 10.65 VGFKK712 pKa = 9.95 TEE714 pKa = 4.18 NEE716 pKa = 4.34 SSVSTMEE723 pKa = 4.2 FLQQRR728 pKa = 11.84 AVLGRR733 pKa = 11.84 PVLYY737 pKa = 9.62 PDD739 pKa = 3.63 RR740 pKa = 11.84 VSLLTAEE747 pKa = 5.03 KK748 pKa = 9.92 PRR750 pKa = 11.84 EE751 pKa = 4.02 AKK753 pKa = 10.85 GPVEE757 pKa = 4.78 KK758 pKa = 10.19 MSEE761 pKa = 4.2 LLALAYY767 pKa = 10.28 DD768 pKa = 4.27 LGNRR772 pKa = 11.84 VPNPHH777 pKa = 6.6 MLLPILYY784 pKa = 10.07 ALYY787 pKa = 10.09 LFCAAKK793 pKa = 8.99 VTLKK797 pKa = 10.48 ISTGAARR804 pKa = 11.84 KK805 pKa = 9.55 LYY807 pKa = 9.7 EE808 pKa = 4.42 DD809 pKa = 3.74 KK810 pKa = 11.26 EE811 pKa = 4.36 FIEE814 pKa = 5.28 RR815 pKa = 11.84 IFGRR819 pKa = 11.84 GIIEE823 pKa = 4.04 QDD825 pKa = 3.34 PKK827 pKa = 11.31 EE828 pKa = 4.3 CTHH831 pKa = 6.53 DD832 pKa = 3.27 TFFFSFIAPLSYY844 pKa = 10.66 AWVEE848 pKa = 4.12 GGPMLPPPSYY858 pKa = 9.87 RR859 pKa = 11.84 RR860 pKa = 11.84 KK861 pKa = 10.32 DD862 pKa = 3.45 GTYY865 pKa = 9.56 SPVPNFYY872 pKa = 10.61 SLRR875 pKa = 11.84 GAYY878 pKa = 9.97 AKK880 pKa = 9.91 TFLFHH885 pKa = 7.58 LSLTSNMWKK894 pKa = 10.23 EE895 pKa = 3.79 LMRR898 pKa = 11.84 RR899 pKa = 11.84 DD900 pKa = 3.56 EE901 pKa = 4.39 EE902 pKa = 4.36 GAIIDD907 pKa = 4.05 ASLLLDD913 pKa = 4.16 YY914 pKa = 11.11 EE915 pKa = 4.44 LLDD918 pKa = 4.58 DD919 pKa = 4.94 RR920 pKa = 11.84 NVLMGVHH927 pKa = 6.36 LAEE930 pKa = 4.4 TLSKK934 pKa = 10.49 FKK936 pKa = 10.3 TVSDD940 pKa = 3.53 VDD942 pKa = 3.21 IEE944 pKa = 4.37 RR945 pKa = 11.84 EE946 pKa = 3.97 YY947 pKa = 10.54 RR948 pKa = 11.84 TALPQMAAALHH959 pKa = 6.95 AYY961 pKa = 9.74 QNLRR965 pKa = 11.84 ANQRR969 pKa = 11.84 SLDD972 pKa = 3.72 AAAKK976 pKa = 9.21 LHH978 pKa = 6.08 AAGVTLPSAIIKK990 pKa = 10.28 GLEE993 pKa = 3.84 AEE995 pKa = 4.46 TKK997 pKa = 10.21 IKK999 pKa = 9.65 GTLRR1003 pKa = 11.84 SVASSPFEE1011 pKa = 4.27 EE1012 pKa = 4.57 IVRR1015 pKa = 11.84 ADD1017 pKa = 3.63 KK1018 pKa = 10.74 LALMLLEE1025 pKa = 4.63 SFRR1028 pKa = 11.84 SYY1030 pKa = 10.94 KK1031 pKa = 9.9 KK1032 pKa = 9.44 NRR1034 pKa = 11.84 RR1035 pKa = 11.84 LRR1037 pKa = 11.84 VHH1039 pKa = 6.86 PEE1041 pKa = 4.0 DD1042 pKa = 4.19 YY1043 pKa = 10.88 SCLYY1047 pKa = 8.25 YY1048 pKa = 10.86 CEE1050 pKa = 4.38 WEE1052 pKa = 4.19 EE1053 pKa = 4.25 GQLITPSTAYY1063 pKa = 9.92 EE1064 pKa = 3.98 VIYY1067 pKa = 10.63 EE1068 pKa = 4.17 NGVTLSPDD1076 pKa = 3.24 ANKK1079 pKa = 10.82 GSIASVLMAYY1089 pKa = 10.56 LGTRR1093 pKa = 11.84 SGFQPHH1099 pKa = 7.15 GDD1101 pKa = 3.02 PFMQKK1106 pKa = 7.4 VQRR1109 pKa = 11.84 AFKK1112 pKa = 10.68 DD1113 pKa = 4.03 FVRR1116 pKa = 11.84 GDD1118 pKa = 3.66 EE1119 pKa = 4.11 VLEE1122 pKa = 4.63 AGLKK1126 pKa = 8.73 VLRR1129 pKa = 11.84 HH1130 pKa = 5.35 APEE1133 pKa = 5.47 LIRR1136 pKa = 11.84 EE1137 pKa = 4.52 FSTAVGLSKK1146 pKa = 10.72 AQEE1149 pKa = 3.9 NRR1151 pKa = 11.84 LIALAPTYY1159 pKa = 10.67 AGSKK1163 pKa = 7.26 YY1164 pKa = 7.7 TYY1166 pKa = 10.12 HH1167 pKa = 7.36 RR1168 pKa = 11.84 IIYY1171 pKa = 9.71 SPRR1174 pKa = 11.84 QYY1176 pKa = 11.46 FMVNSDD1182 pKa = 3.73 PYY1184 pKa = 11.11 ALRR1187 pKa = 11.84 SHH1189 pKa = 7.53 ISCQHH1194 pKa = 4.83 QGGPLLIALSCVQAQEE1210 pKa = 3.9 WLSYY1214 pKa = 10.63 NVPTSKK1220 pKa = 11.01 GEE1222 pKa = 4.08 RR1223 pKa = 11.84 IKK1225 pKa = 11.14 VYY1227 pKa = 10.48 LPWRR1231 pKa = 11.84 TLEE1234 pKa = 3.71 RR1235 pKa = 11.84 LGRR1238 pKa = 11.84 LKK1240 pKa = 10.23 QACC1243 pKa = 3.63
Molecular weight: 140.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.758
IPC2_protein 8.024
IPC_protein 7.849
Toseland 7.775
ProMoST 8.404
Dawson 8.609
Bjellqvist 8.799
Wikipedia 8.624
Rodwell 8.668
Grimsley 7.892
Solomon 8.653
Lehninger 8.639
Nozaki 8.843
DTASelect 8.609
Thurlkill 8.653
EMBOSS 8.77
Sillero 8.902
Patrickios 4.266
IPC_peptide 8.653
IPC2_peptide 7.658
IPC2.peptide.svr19 7.856
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
0
10
8024
334
1310
802.4
89.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.328 ± 0.199
1.309 ± 0.221
5.309 ± 0.389
4.723 ± 0.23
3.801 ± 0.257
5.77 ± 0.263
2.493 ± 0.234
5.82 ± 0.307
3.863 ± 0.294
10.057 ± 0.343
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.168 ± 0.138
3.926 ± 0.239
5.733 ± 0.258
3.739 ± 0.237
5.558 ± 0.221
8.923 ± 0.434
6.381 ± 0.297
7.814 ± 0.373
1.346 ± 0.212
3.938 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here