Niastella vici
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7808 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V9G7W4|A0A1V9G7W4_9BACT Uncharacterized protein OS=Niastella vici OX=1703345 GN=A3860_13885 PE=4 SV=1
MM1 pKa = 7.66 NILINQQSRR10 pKa = 11.84 DD11 pKa = 3.7 IPDD14 pKa = 3.64 SCSLEE19 pKa = 3.92 QLLTQVLGIPVAGLAVAVDD38 pKa = 3.57 QTVIPSTEE46 pKa = 3.42 WPAYY50 pKa = 7.21 MLCEE54 pKa = 4.6 ADD56 pKa = 3.5 NVMLIRR62 pKa = 11.84 ATQGGG67 pKa = 3.72
Molecular weight: 7.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.792
IPC2_protein 3.923
IPC_protein 3.706
Toseland 3.541
ProMoST 3.923
Dawson 3.719
Bjellqvist 3.897
Wikipedia 3.706
Rodwell 3.567
Grimsley 3.465
Solomon 3.668
Lehninger 3.63
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.846
Patrickios 1.888
IPC_peptide 3.668
IPC2_peptide 3.808
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A1V9G8Z4|A0A1V9G8Z4_9BACT Uncharacterized protein OS=Niastella vici OX=1703345 GN=A3860_01245 PE=4 SV=1
MM1 pKa = 7.45 TGTMPLKK8 pKa = 10.48 LYY10 pKa = 10.99 SPGGRR15 pKa = 11.84 LNWLMRR21 pKa = 11.84 VPRR24 pKa = 11.84 KK25 pKa = 10.14 RR26 pKa = 11.84 GTDD29 pKa = 3.43 VEE31 pKa = 4.38 NEE33 pKa = 4.3 CIFFISATIFNRR45 pKa = 11.84 EE46 pKa = 3.88 QVSGVTTSKK55 pKa = 9.84 TIRR58 pKa = 11.84 CLLRR62 pKa = 11.84 TGFYY66 pKa = 10.31 HH67 pKa = 6.97 IRR69 pKa = 11.84 SS70 pKa = 3.79
Molecular weight: 8.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.692
IPC_protein 10.584
Toseland 10.643
ProMoST 10.394
Dawson 10.76
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.906
Grimsley 10.818
Solomon 10.891
Lehninger 10.862
Nozaki 10.657
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.701
Patrickios 10.716
IPC_peptide 10.891
IPC2_peptide 9.765
IPC2.peptide.svr19 8.44
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7808
0
7808
2869664
26
4088
367.5
41.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.635 ± 0.029
0.905 ± 0.01
5.061 ± 0.019
5.21 ± 0.032
4.741 ± 0.018
6.981 ± 0.031
1.893 ± 0.014
6.832 ± 0.024
6.504 ± 0.03
9.221 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.265 ± 0.012
5.786 ± 0.028
4.075 ± 0.016
4.015 ± 0.018
4.169 ± 0.019
6.237 ± 0.028
6.326 ± 0.037
6.448 ± 0.019
1.402 ± 0.013
4.293 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here