Amino acid dipepetide frequency for Gordonia phage Bachita

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.415AlaAla: 10.415 ± 0.895
0.507AlaCys: 0.507 ± 0.166
6.864AlaAsp: 6.864 ± 0.491
6.323AlaGlu: 6.323 ± 0.592
2.874AlaPhe: 2.874 ± 0.414
7.913AlaGly: 7.913 ± 0.533
2.13AlaHis: 2.13 ± 0.367
4.531AlaIle: 4.531 ± 0.46
3.889AlaLys: 3.889 ± 0.537
7.913AlaLeu: 7.913 ± 0.504
2.502AlaMet: 2.502 ± 0.283
3.179AlaAsn: 3.179 ± 0.35
4.159AlaPro: 4.159 ± 0.405
4.531AlaGln: 4.531 ± 0.728
6.898AlaArg: 6.898 ± 0.733
4.666AlaSer: 4.666 ± 0.396
4.971AlaThr: 4.971 ± 0.496
7.169AlaVal: 7.169 ± 0.571
1.894AlaTrp: 1.894 ± 0.294
2.198AlaTyr: 2.198 ± 0.263
0.0AlaXaa: 0.0 ± 0.0
Cys
0.71CysAla: 0.71 ± 0.193
0.203CysCys: 0.203 ± 0.091
0.947CysAsp: 0.947 ± 0.229
0.778CysGlu: 0.778 ± 0.186
0.372CysPhe: 0.372 ± 0.106
1.014CysGly: 1.014 ± 0.242
0.507CysHis: 0.507 ± 0.149
0.406CysIle: 0.406 ± 0.12
0.44CysLys: 0.44 ± 0.134
0.473CysLeu: 0.473 ± 0.152
0.034CysMet: 0.034 ± 0.031
0.44CysAsn: 0.44 ± 0.144
0.473CysPro: 0.473 ± 0.161
0.44CysGln: 0.44 ± 0.124
0.642CysArg: 0.642 ± 0.161
0.575CysSer: 0.575 ± 0.149
0.473CysThr: 0.473 ± 0.146
0.71CysVal: 0.71 ± 0.176
0.169CysTrp: 0.169 ± 0.071
0.135CysTyr: 0.135 ± 0.061
0.0CysXaa: 0.0 ± 0.0
Asp
6.222AspAla: 6.222 ± 0.516
0.609AspCys: 0.609 ± 0.189
4.903AspAsp: 4.903 ± 0.453
5.038AspGlu: 5.038 ± 0.396
2.604AspPhe: 2.604 ± 0.253
5.816AspGly: 5.816 ± 0.469
1.725AspHis: 1.725 ± 0.201
3.28AspIle: 3.28 ± 0.351
2.671AspLys: 2.671 ± 0.297
5.85AspLeu: 5.85 ± 0.415
1.894AspMet: 1.894 ± 0.3
2.13AspAsn: 2.13 ± 0.308
4.362AspPro: 4.362 ± 0.439
2.063AspGln: 2.063 ± 0.279
5.275AspArg: 5.275 ± 0.506
2.773AspSer: 2.773 ± 0.33
4.294AspThr: 4.294 ± 0.437
5.309AspVal: 5.309 ± 0.404
1.42AspTrp: 1.42 ± 0.254
2.807AspTyr: 2.807 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
7.101GluAla: 7.101 ± 0.817
0.642GluCys: 0.642 ± 0.208
3.922GluAsp: 3.922 ± 0.331
4.362GluGlu: 4.362 ± 0.44
2.435GluPhe: 2.435 ± 0.257
4.666GluGly: 4.666 ± 0.342
1.42GluHis: 1.42 ± 0.224
4.058GluIle: 4.058 ± 0.384
2.739GluLys: 2.739 ± 0.385
6.391GluLeu: 6.391 ± 0.473
2.063GluMet: 2.063 ± 0.229
1.995GluAsn: 1.995 ± 0.216
3.043GluPro: 3.043 ± 0.524
2.638GluGln: 2.638 ± 0.307
4.633GluArg: 4.633 ± 0.374
3.381GluSer: 3.381 ± 0.346
3.584GluThr: 3.584 ± 0.33
4.835GluVal: 4.835 ± 0.554
2.164GluTrp: 2.164 ± 0.35
2.164GluTyr: 2.164 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
3.28PheAla: 3.28 ± 0.354
0.44PheCys: 0.44 ± 0.126
3.111PheAsp: 3.111 ± 0.243
2.367PheGlu: 2.367 ± 0.299
0.676PhePhe: 0.676 ± 0.153
2.232PheGly: 2.232 ± 0.3
0.676PheHis: 0.676 ± 0.134
1.488PheIle: 1.488 ± 0.249
1.116PheLys: 1.116 ± 0.163
2.063PheLeu: 2.063 ± 0.323
1.15PheMet: 1.15 ± 0.204
1.589PheAsn: 1.589 ± 0.214
1.589PhePro: 1.589 ± 0.212
0.812PheGln: 0.812 ± 0.171
1.589PheArg: 1.589 ± 0.233
1.995PheSer: 1.995 ± 0.227
2.13PheThr: 2.13 ± 0.279
2.029PheVal: 2.029 ± 0.319
0.812PheTrp: 0.812 ± 0.188
0.913PheTyr: 0.913 ± 0.167
0.0PheXaa: 0.0 ± 0.0
Gly
5.579GlyAla: 5.579 ± 0.569
0.812GlyCys: 0.812 ± 0.196
5.478GlyAsp: 5.478 ± 0.517
4.971GlyGlu: 4.971 ± 0.333
2.84GlyPhe: 2.84 ± 0.309
6.087GlyGly: 6.087 ± 0.736
1.894GlyHis: 1.894 ± 0.277
3.922GlyIle: 3.922 ± 0.367
3.415GlyLys: 3.415 ± 0.336
6.053GlyLeu: 6.053 ± 0.422
2.13GlyMet: 2.13 ± 0.268
2.739GlyAsn: 2.739 ± 0.343
3.855GlyPro: 3.855 ± 0.388
3.145GlyGln: 3.145 ± 0.307
5.14GlyArg: 5.14 ± 0.414
4.464GlySer: 4.464 ± 0.448
4.903GlyThr: 4.903 ± 0.537
6.188GlyVal: 6.188 ± 0.478
2.198GlyTrp: 2.198 ± 0.286
2.299GlyTyr: 2.299 ± 0.288
0.0GlyXaa: 0.0 ± 0.0
His
1.758HisAla: 1.758 ± 0.268
0.338HisCys: 0.338 ± 0.115
1.217HisAsp: 1.217 ± 0.269
1.589HisGlu: 1.589 ± 0.243
0.947HisPhe: 0.947 ± 0.151
1.961HisGly: 1.961 ± 0.315
0.541HisHis: 0.541 ± 0.137
1.014HisIle: 1.014 ± 0.189
0.676HisLys: 0.676 ± 0.149
2.097HisLeu: 2.097 ± 0.27
0.338HisMet: 0.338 ± 0.095
0.812HisAsn: 0.812 ± 0.186
1.353HisPro: 1.353 ± 0.263
0.981HisGln: 0.981 ± 0.2
1.927HisArg: 1.927 ± 0.274
1.386HisSer: 1.386 ± 0.245
1.285HisThr: 1.285 ± 0.194
1.927HisVal: 1.927 ± 0.272
0.473HisTrp: 0.473 ± 0.156
0.744HisTyr: 0.744 ± 0.164
0.0HisXaa: 0.0 ± 0.0
Ile
4.802IleAla: 4.802 ± 0.398
0.744IleCys: 0.744 ± 0.174
3.99IleAsp: 3.99 ± 0.395
3.686IleGlu: 3.686 ± 0.324
0.947IlePhe: 0.947 ± 0.177
3.111IleGly: 3.111 ± 0.278
1.116IleHis: 1.116 ± 0.216
1.961IleIle: 1.961 ± 0.341
2.266IleLys: 2.266 ± 0.274
3.077IleLeu: 3.077 ± 0.312
0.507IleMet: 0.507 ± 0.111
1.86IleAsn: 1.86 ± 0.308
2.367IlePro: 2.367 ± 0.303
1.792IleGln: 1.792 ± 0.262
3.956IleArg: 3.956 ± 0.425
3.111IleSer: 3.111 ± 0.34
3.28IleThr: 3.28 ± 0.308
3.145IleVal: 3.145 ± 0.379
1.014IleTrp: 1.014 ± 0.179
1.353IleTyr: 1.353 ± 0.208
0.0IleXaa: 0.0 ± 0.0
Lys
3.483LysAla: 3.483 ± 0.38
0.575LysCys: 0.575 ± 0.188
2.468LysAsp: 2.468 ± 0.307
2.232LysGlu: 2.232 ± 0.287
1.522LysPhe: 1.522 ± 0.307
3.077LysGly: 3.077 ± 0.389
0.981LysHis: 0.981 ± 0.172
1.725LysIle: 1.725 ± 0.239
2.198LysLys: 2.198 ± 0.328
3.753LysLeu: 3.753 ± 0.37
1.251LysMet: 1.251 ± 0.252
1.42LysAsn: 1.42 ± 0.234
2.333LysPro: 2.333 ± 0.275
1.15LysGln: 1.15 ± 0.252
3.043LysArg: 3.043 ± 0.303
2.604LysSer: 2.604 ± 0.329
1.961LysThr: 1.961 ± 0.246
3.652LysVal: 3.652 ± 0.313
0.71LysTrp: 0.71 ± 0.153
0.947LysTyr: 0.947 ± 0.165
0.0LysXaa: 0.0 ± 0.0
Leu
7.033LeuAla: 7.033 ± 0.694
0.879LeuCys: 0.879 ± 0.204
5.884LeuAsp: 5.884 ± 0.387
5.884LeuGlu: 5.884 ± 0.467
2.198LeuPhe: 2.198 ± 0.287
6.053LeuGly: 6.053 ± 0.513
1.758LeuHis: 1.758 ± 0.276
3.517LeuIle: 3.517 ± 0.345
2.739LeuLys: 2.739 ± 0.304
5.106LeuLeu: 5.106 ± 0.465
1.522LeuMet: 1.522 ± 0.203
3.348LeuAsn: 3.348 ± 0.351
3.787LeuPro: 3.787 ± 0.39
3.043LeuGln: 3.043 ± 0.359
5.444LeuArg: 5.444 ± 0.362
4.092LeuSer: 4.092 ± 0.35
4.903LeuThr: 4.903 ± 0.397
5.207LeuVal: 5.207 ± 0.469
1.319LeuTrp: 1.319 ± 0.19
2.13LeuTyr: 2.13 ± 0.251
0.0LeuXaa: 0.0 ± 0.0
Met
2.874MetAla: 2.874 ± 0.315
0.135MetCys: 0.135 ± 0.07
1.386MetAsp: 1.386 ± 0.245
1.353MetGlu: 1.353 ± 0.236
0.642MetPhe: 0.642 ± 0.143
1.522MetGly: 1.522 ± 0.188
0.642MetHis: 0.642 ± 0.164
1.082MetIle: 1.082 ± 0.209
0.812MetLys: 0.812 ± 0.178
1.623MetLeu: 1.623 ± 0.233
0.473MetMet: 0.473 ± 0.138
1.251MetAsn: 1.251 ± 0.17
1.454MetPro: 1.454 ± 0.196
0.879MetGln: 0.879 ± 0.176
1.826MetArg: 1.826 ± 0.268
2.671MetSer: 2.671 ± 0.282
2.198MetThr: 2.198 ± 0.306
1.522MetVal: 1.522 ± 0.242
0.304MetTrp: 0.304 ± 0.109
0.575MetTyr: 0.575 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
3.551AsnAla: 3.551 ± 0.462
0.473AsnCys: 0.473 ± 0.143
2.468AsnAsp: 2.468 ± 0.23
2.435AsnGlu: 2.435 ± 0.28
0.947AsnPhe: 0.947 ± 0.213
3.483AsnGly: 3.483 ± 0.367
0.947AsnHis: 0.947 ± 0.173
1.522AsnIle: 1.522 ± 0.303
1.488AsnLys: 1.488 ± 0.304
3.077AsnLeu: 3.077 ± 0.395
0.575AsnMet: 0.575 ± 0.146
1.116AsnAsn: 1.116 ± 0.212
2.097AsnPro: 2.097 ± 0.225
1.488AsnGln: 1.488 ± 0.215
2.401AsnArg: 2.401 ± 0.293
1.995AsnSer: 1.995 ± 0.237
2.063AsnThr: 2.063 ± 0.305
2.468AsnVal: 2.468 ± 0.247
0.642AsnTrp: 0.642 ± 0.142
0.981AsnTyr: 0.981 ± 0.2
0.0AsnXaa: 0.0 ± 0.0
Pro
5.309ProAla: 5.309 ± 0.498
0.304ProCys: 0.304 ± 0.115
3.821ProAsp: 3.821 ± 0.384
3.889ProGlu: 3.889 ± 0.467
2.029ProPhe: 2.029 ± 0.262
4.43ProGly: 4.43 ± 0.414
0.778ProHis: 0.778 ± 0.186
2.299ProIle: 2.299 ± 0.257
2.367ProLys: 2.367 ± 0.314
2.942ProLeu: 2.942 ± 0.336
1.048ProMet: 1.048 ± 0.222
1.589ProAsn: 1.589 ± 0.217
2.097ProPro: 2.097 ± 0.29
1.488ProGln: 1.488 ± 0.215
2.705ProArg: 2.705 ± 0.346
2.773ProSer: 2.773 ± 0.347
3.043ProThr: 3.043 ± 0.419
3.246ProVal: 3.246 ± 0.347
1.048ProTrp: 1.048 ± 0.172
1.454ProTyr: 1.454 ± 0.236
0.0ProXaa: 0.0 ± 0.0
Gln
3.415GlnAla: 3.415 ± 0.433
0.372GlnCys: 0.372 ± 0.114
1.894GlnAsp: 1.894 ± 0.256
2.063GlnGlu: 2.063 ± 0.303
0.947GlnPhe: 0.947 ± 0.195
2.705GlnGly: 2.705 ± 0.274
0.778GlnHis: 0.778 ± 0.186
2.063GlnIle: 2.063 ± 0.256
1.657GlnLys: 1.657 ± 0.346
3.72GlnLeu: 3.72 ± 0.378
1.251GlnMet: 1.251 ± 0.223
1.691GlnAsn: 1.691 ± 0.295
1.555GlnPro: 1.555 ± 0.23
1.691GlnGln: 1.691 ± 0.359
2.671GlnArg: 2.671 ± 0.378
1.961GlnSer: 1.961 ± 0.386
1.623GlnThr: 1.623 ± 0.287
2.266GlnVal: 2.266 ± 0.273
0.879GlnTrp: 0.879 ± 0.139
0.913GlnTyr: 0.913 ± 0.153
0.0GlnXaa: 0.0 ± 0.0
Arg
6.29ArgAla: 6.29 ± 0.485
0.778ArgCys: 0.778 ± 0.202
4.666ArgAsp: 4.666 ± 0.391
5.343ArgGlu: 5.343 ± 0.449
2.063ArgPhe: 2.063 ± 0.253
4.835ArgGly: 4.835 ± 0.426
1.758ArgHis: 1.758 ± 0.262
3.212ArgIle: 3.212 ± 0.276
3.212ArgLys: 3.212 ± 0.338
5.377ArgLeu: 5.377 ± 0.426
1.758ArgMet: 1.758 ± 0.253
2.739ArgAsn: 2.739 ± 0.293
2.705ArgPro: 2.705 ± 0.315
2.638ArgGln: 2.638 ± 0.304
5.985ArgArg: 5.985 ± 0.616
3.956ArgSer: 3.956 ± 0.345
3.99ArgThr: 3.99 ± 0.416
5.579ArgVal: 5.579 ± 0.501
1.657ArgTrp: 1.657 ± 0.265
2.367ArgTyr: 2.367 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
5.579SerAla: 5.579 ± 0.481
0.44SerCys: 0.44 ± 0.126
3.889SerAsp: 3.889 ± 0.357
3.821SerGlu: 3.821 ± 0.358
1.927SerPhe: 1.927 ± 0.214
5.14SerGly: 5.14 ± 0.548
1.217SerHis: 1.217 ± 0.237
3.077SerIle: 3.077 ± 0.31
2.13SerLys: 2.13 ± 0.283
4.092SerLeu: 4.092 ± 0.347
1.691SerMet: 1.691 ± 0.249
1.927SerAsn: 1.927 ± 0.292
2.299SerPro: 2.299 ± 0.238
1.522SerGln: 1.522 ± 0.23
3.956SerArg: 3.956 ± 0.371
3.28SerSer: 3.28 ± 0.337
2.976SerThr: 2.976 ± 0.323
4.599SerVal: 4.599 ± 0.427
1.353SerTrp: 1.353 ± 0.215
1.86SerTyr: 1.86 ± 0.25
0.0SerXaa: 0.0 ± 0.0
Thr
6.391ThrAla: 6.391 ± 0.473
0.575ThrCys: 0.575 ± 0.146
4.294ThrAsp: 4.294 ± 0.35
4.024ThrGlu: 4.024 ± 0.287
1.995ThrPhe: 1.995 ± 0.255
4.802ThrGly: 4.802 ± 0.427
1.184ThrHis: 1.184 ± 0.188
2.976ThrIle: 2.976 ± 0.386
2.84ThrLys: 2.84 ± 0.393
3.889ThrLeu: 3.889 ± 0.337
1.488ThrMet: 1.488 ± 0.222
1.927ThrAsn: 1.927 ± 0.236
3.889ThrPro: 3.889 ± 0.392
1.725ThrGln: 1.725 ± 0.278
3.584ThrArg: 3.584 ± 0.381
3.787ThrSer: 3.787 ± 0.513
3.077ThrThr: 3.077 ± 0.47
3.821ThrVal: 3.821 ± 0.373
1.014ThrTrp: 1.014 ± 0.166
1.657ThrTyr: 1.657 ± 0.254
0.0ThrXaa: 0.0 ± 0.0
Val
7.27ValAla: 7.27 ± 0.592
0.642ValCys: 0.642 ± 0.158
6.087ValAsp: 6.087 ± 0.384
4.937ValGlu: 4.937 ± 0.477
2.57ValPhe: 2.57 ± 0.301
5.444ValGly: 5.444 ± 0.313
1.758ValHis: 1.758 ± 0.245
3.686ValIle: 3.686 ± 0.435
2.705ValLys: 2.705 ± 0.258
4.768ValLeu: 4.768 ± 0.453
2.063ValMet: 2.063 ± 0.234
2.367ValAsn: 2.367 ± 0.343
3.246ValPro: 3.246 ± 0.353
2.232ValGln: 2.232 ± 0.245
5.241ValArg: 5.241 ± 0.335
4.328ValSer: 4.328 ± 0.472
5.038ValThr: 5.038 ± 0.537
5.681ValVal: 5.681 ± 0.492
1.488ValTrp: 1.488 ± 0.232
1.961ValTyr: 1.961 ± 0.318
0.0ValXaa: 0.0 ± 0.0
Trp
1.758TrpAla: 1.758 ± 0.248
0.237TrpCys: 0.237 ± 0.112
1.522TrpAsp: 1.522 ± 0.213
1.522TrpGlu: 1.522 ± 0.263
0.71TrpPhe: 0.71 ± 0.158
1.454TrpGly: 1.454 ± 0.278
0.879TrpHis: 0.879 ± 0.192
1.217TrpIle: 1.217 ± 0.188
0.71TrpLys: 0.71 ± 0.154
1.589TrpLeu: 1.589 ± 0.265
0.845TrpMet: 0.845 ± 0.159
0.879TrpAsn: 0.879 ± 0.177
0.981TrpPro: 0.981 ± 0.237
0.812TrpGln: 0.812 ± 0.164
1.386TrpArg: 1.386 ± 0.246
1.285TrpSer: 1.285 ± 0.27
1.657TrpThr: 1.657 ± 0.242
1.454TrpVal: 1.454 ± 0.211
0.507TrpTrp: 0.507 ± 0.154
0.473TrpTyr: 0.473 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.705TyrAla: 2.705 ± 0.301
0.237TyrCys: 0.237 ± 0.093
2.232TyrAsp: 2.232 ± 0.36
1.623TyrGlu: 1.623 ± 0.293
0.812TyrPhe: 0.812 ± 0.199
2.367TyrGly: 2.367 ± 0.283
0.676TyrHis: 0.676 ± 0.156
1.048TyrIle: 1.048 ± 0.205
1.014TyrLys: 1.014 ± 0.196
1.995TyrLeu: 1.995 ± 0.26
0.609TyrMet: 0.609 ± 0.158
1.217TyrAsn: 1.217 ± 0.22
1.014TyrPro: 1.014 ± 0.231
1.082TyrGln: 1.082 ± 0.192
2.604TyrArg: 2.604 ± 0.359
1.691TyrSer: 1.691 ± 0.263
1.488TyrThr: 1.488 ± 0.206
2.705TyrVal: 2.705 ± 0.405
0.812TyrTrp: 0.812 ± 0.165
0.541TyrTyr: 0.541 ± 0.126
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 182 proteins (29574 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski