Wuhan insect virus 22

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLW1|A0A1L3KLW1_9VIRU RdRp OS=Wuhan insect virus 22 OX=1923726 PE=4 SV=1
MM1 pKa = 7.67AMNNSATTSQGQQMVMGSQNSDD23 pKa = 2.6SGLRR27 pKa = 11.84NRR29 pKa = 11.84RR30 pKa = 11.84WMRR33 pKa = 11.84DD34 pKa = 2.89GQRR37 pKa = 11.84QLDD40 pKa = 3.77RR41 pKa = 11.84ALDD44 pKa = 3.87KK45 pKa = 11.1DD46 pKa = 3.51WEE48 pKa = 4.41KK49 pKa = 11.22NRR51 pKa = 11.84DD52 pKa = 2.91WSSQVDD58 pKa = 4.11VEE60 pKa = 4.38NKK62 pKa = 9.52IASKK66 pKa = 9.66TPTQQPVSRR75 pKa = 11.84TRR77 pKa = 11.84RR78 pKa = 11.84NRR80 pKa = 11.84NRR82 pKa = 11.84AVQWKK87 pKa = 6.71TNKK90 pKa = 10.0KK91 pKa = 9.21ISEE94 pKa = 4.25DD95 pKa = 3.26ASRR98 pKa = 11.84IDD100 pKa = 3.31PSKK103 pKa = 9.51YY104 pKa = 9.77KK105 pKa = 10.63SVATSEE111 pKa = 5.08LKK113 pKa = 10.74DD114 pKa = 3.46EE115 pKa = 4.38PLYY118 pKa = 11.25NIVNGSRR125 pKa = 11.84PNKK128 pKa = 9.77RR129 pKa = 11.84LYY131 pKa = 10.73SVNFDD136 pKa = 3.7FSSFPLLCDD145 pKa = 2.83MTYY148 pKa = 10.74RR149 pKa = 11.84EE150 pKa = 5.01LRR152 pKa = 11.84LSNPRR157 pKa = 11.84IKK159 pKa = 10.4RR160 pKa = 11.84EE161 pKa = 3.78MPFCSFLHH169 pKa = 6.77SMTSCLNAWIIQTVKK184 pKa = 10.59KK185 pKa = 10.36INSEE189 pKa = 3.92DD190 pKa = 3.57RR191 pKa = 11.84FSEE194 pKa = 3.93EE195 pKa = 3.91RR196 pKa = 11.84DD197 pKa = 3.39PAEE200 pKa = 4.87LIPLEE205 pKa = 4.07MLIPQPIAEE214 pKa = 4.42YY215 pKa = 10.37LSLVNTIVTSSGDD228 pKa = 3.49TVRR231 pKa = 11.84VNFPDD236 pKa = 3.75AGLPQLSLPEE246 pKa = 6.29DD247 pKa = 3.89GDD249 pKa = 3.94KK250 pKa = 11.45PEE252 pKa = 4.35VPPGSFGDD260 pKa = 3.74VTSQNHH266 pKa = 5.41NAYY269 pKa = 8.77EE270 pKa = 4.67CYY272 pKa = 10.12VSPLITRR279 pKa = 11.84RR280 pKa = 11.84FLEE283 pKa = 3.94ASRR286 pKa = 11.84DD287 pKa = 3.79NNPNYY292 pKa = 10.29HH293 pKa = 7.09PLPPEE298 pKa = 3.9RR299 pKa = 11.84VTAGTTPTRR308 pKa = 11.84NLLGYY313 pKa = 9.94YY314 pKa = 9.08PVEE317 pKa = 4.06KK318 pKa = 10.71VPPEE322 pKa = 3.91GVAILSGYY330 pKa = 7.43EE331 pKa = 3.97FPEE334 pKa = 4.22TDD336 pKa = 3.84DD337 pKa = 3.27MTGRR341 pKa = 11.84LQLVPSLMLEE351 pKa = 4.09VSNSLFRR358 pKa = 11.84CQRR361 pKa = 11.84DD362 pKa = 3.31VKK364 pKa = 8.67MTKK367 pKa = 9.9FHH369 pKa = 7.37DD370 pKa = 3.61IVRR373 pKa = 11.84KK374 pKa = 8.61PVQTGLVFAKK384 pKa = 9.19VTSAPQINVSTLFNISSNLHH404 pKa = 5.46GPMAFGNVTASQAALFAYY422 pKa = 9.83RR423 pKa = 11.84RR424 pKa = 11.84EE425 pKa = 4.25RR426 pKa = 11.84TSSAKK431 pKa = 9.97GLCYY435 pKa = 10.02VAANNAVPAGWGATINNNFNMAAPFHH461 pKa = 7.24AYY463 pKa = 9.1TGVDD467 pKa = 3.72GPQFRR472 pKa = 11.84SDD474 pKa = 3.12QHH476 pKa = 5.72QAFPPDD482 pKa = 3.58TEE484 pKa = 4.07RR485 pKa = 11.84ATLIGDD491 pKa = 3.34WLKK494 pKa = 11.05NFRR497 pKa = 11.84LSQRR501 pKa = 3.37

Molecular weight: 56.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLW1|A0A1L3KLW1_9VIRU RdRp OS=Wuhan insect virus 22 OX=1923726 PE=4 SV=1
MM1 pKa = 7.55AVRR4 pKa = 11.84IEE6 pKa = 3.98VEE8 pKa = 4.14QRR10 pKa = 11.84QDD12 pKa = 2.57WVLPSHH18 pKa = 6.83APYY21 pKa = 9.72TISSFEE27 pKa = 3.99YY28 pKa = 9.64PEE30 pKa = 4.4RR31 pKa = 11.84PTIQEE36 pKa = 3.95SKK38 pKa = 10.72IKK40 pKa = 10.54IEE42 pKa = 5.01DD43 pKa = 3.89GEE45 pKa = 4.45LLPNGKK51 pKa = 9.46GFPWRR56 pKa = 11.84MEE58 pKa = 3.96PPSVSFKK65 pKa = 10.78VYY67 pKa = 10.12RR68 pKa = 11.84AMVNHH73 pKa = 6.93GLSDD77 pKa = 3.7VANHH81 pKa = 6.06VLRR84 pKa = 11.84TKK86 pKa = 10.06KK87 pKa = 10.13RR88 pKa = 11.84SYY90 pKa = 9.09ITPQAIWNGVLEE102 pKa = 4.58FGEE105 pKa = 4.37ALPSKK110 pKa = 10.1LRR112 pKa = 11.84KK113 pKa = 9.64LPEE116 pKa = 3.73YY117 pKa = 10.86LLALDD122 pKa = 4.22EE123 pKa = 4.5LRR125 pKa = 11.84IEE127 pKa = 4.65IDD129 pKa = 3.13TKK131 pKa = 11.46DD132 pKa = 3.79RR133 pKa = 11.84ITPFTMEE140 pKa = 4.59EE141 pKa = 4.3GAKK144 pKa = 9.16TLPQGTSPGLPYY156 pKa = 10.47INTHH160 pKa = 6.25PGWKK164 pKa = 9.97KK165 pKa = 10.63GDD167 pKa = 3.32ISLKK171 pKa = 10.51YY172 pKa = 9.59MPAFNNYY179 pKa = 6.42WDD181 pKa = 4.13RR182 pKa = 11.84VGQGLPVAPLPDD194 pKa = 3.55CAAFARR200 pKa = 11.84SHH202 pKa = 6.37ISSPDD207 pKa = 3.39VNKK210 pKa = 9.7VRR212 pKa = 11.84PVWAYY217 pKa = 9.83PMMAIAQEE225 pKa = 4.3SRR227 pKa = 11.84FVAPLLRR234 pKa = 11.84DD235 pKa = 4.78LIDD238 pKa = 3.5QKK240 pKa = 11.04IGLHH244 pKa = 4.97TAYY247 pKa = 10.16GMEE250 pKa = 4.01MMKK253 pKa = 10.93GGMTWLNGQAMIANARR269 pKa = 11.84QPGVKK274 pKa = 10.16YY275 pKa = 10.91LMTDD279 pKa = 3.45FSGFDD284 pKa = 3.14KK285 pKa = 11.02SLPAWLIRR293 pKa = 11.84DD294 pKa = 3.25IFKK297 pKa = 10.49IIEE300 pKa = 3.9EE301 pKa = 4.35KK302 pKa = 10.72YY303 pKa = 9.86QFNRR307 pKa = 11.84SWNGKK312 pKa = 7.54EE313 pKa = 4.75EE314 pKa = 4.0IPQYY318 pKa = 10.74GAVEE322 pKa = 3.84RR323 pKa = 11.84RR324 pKa = 11.84KK325 pKa = 9.76FRR327 pKa = 11.84RR328 pKa = 11.84LVNYY332 pKa = 8.08FINTPIQNCDD342 pKa = 2.5GRR344 pKa = 11.84RR345 pKa = 11.84FLKK348 pKa = 10.55KK349 pKa = 9.65HH350 pKa = 5.24GVPSGSMFTNIIDD363 pKa = 3.73TFANFVMSRR372 pKa = 11.84TIAKK376 pKa = 10.08LSSNEE381 pKa = 3.66NPIFDD386 pKa = 4.52VYY388 pKa = 11.05FGDD391 pKa = 4.08DD392 pKa = 3.96AIMCFSGDD400 pKa = 3.14TAVDD404 pKa = 3.42VRR406 pKa = 11.84KK407 pKa = 10.31YY408 pKa = 11.14SLAAKK413 pKa = 9.96DD414 pKa = 3.96LFGMTLSPKK423 pKa = 10.08KK424 pKa = 10.54SYY426 pKa = 9.51LTTRR430 pKa = 11.84PDD432 pKa = 3.69NIHH435 pKa = 6.07FLGYY439 pKa = 10.11FNYY442 pKa = 10.35HH443 pKa = 6.04GSPYY447 pKa = 9.97KK448 pKa = 10.55AAEE451 pKa = 3.96EE452 pKa = 5.02LIASMLYY459 pKa = 9.57PQYY462 pKa = 11.26LKK464 pKa = 10.78DD465 pKa = 3.25DD466 pKa = 3.34WAYY469 pKa = 10.46CVSRR473 pKa = 11.84ALGCALASAGMQTDD487 pKa = 4.17VYY489 pKa = 10.68LAAQAVFIFASRR501 pKa = 11.84RR502 pKa = 11.84EE503 pKa = 4.12NKK505 pKa = 9.98FEE507 pKa = 4.34EE508 pKa = 4.66GLEE511 pKa = 3.95LLRR514 pKa = 11.84TNPRR518 pKa = 11.84AKK520 pKa = 10.11RR521 pKa = 11.84HH522 pKa = 4.93MSQMGCSDD530 pKa = 3.91LPLNEE535 pKa = 4.56SYY537 pKa = 10.84FRR539 pKa = 11.84DD540 pKa = 3.28INALVPRR547 pKa = 11.84MDD549 pKa = 3.82CSKK552 pKa = 9.3ITKK555 pKa = 9.33GVNIVII561 pKa = 4.41

Molecular weight: 63.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1062

501

561

531.0

60.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.156 ± 0.372

1.224 ± 0.017

5.461 ± 0.083

5.179 ± 0.123

4.614 ± 0.276

5.556 ± 0.5

1.601 ± 0.133

5.179 ± 0.906

5.367 ± 0.638

8.004 ± 0.143

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.39 ± 0.389

5.838 ± 0.881

6.968 ± 0.273

3.766 ± 0.539

6.874 ± 0.465

7.627 ± 0.886

5.179 ± 0.659

5.744 ± 0.42

1.601 ± 0.133

3.672 ± 0.574
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski