Leptospira phage LE4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Nylescharonvirus; Leptospira virus LE4

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 81 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343LEE5|A0A343LEE5_9CAUD Uncharacterized protein OS=Leptospira phage LE4 OX=2041383 PE=4 SV=1
MM1 pKa = 7.44SNKK4 pKa = 9.72KK5 pKa = 9.94VIQYY9 pKa = 7.12STKK12 pKa = 11.02DD13 pKa = 3.57FLTALADD20 pKa = 3.58IEE22 pKa = 4.6SDD24 pKa = 3.41PEE26 pKa = 4.11LRR28 pKa = 11.84KK29 pKa = 9.9FPARR33 pKa = 11.84FKK35 pKa = 10.35RR36 pKa = 11.84LHH38 pKa = 6.67AGTVDD43 pKa = 4.99AINNNLNAVFNSIILRR59 pKa = 11.84TAFSRR64 pKa = 11.84PILQDD69 pKa = 2.88ILQLIDD75 pKa = 4.08YY76 pKa = 9.44QMSWKK81 pKa = 7.96KK82 pKa = 9.22TSVATIQITVVSSATPYY99 pKa = 10.77VILKK103 pKa = 9.68ADD105 pKa = 4.68CIFGTAGTVDD115 pKa = 3.19QASIQFEE122 pKa = 4.29SRR124 pKa = 11.84EE125 pKa = 4.33DD126 pKa = 3.34IAVPALTTLISRR138 pKa = 11.84TVYY141 pKa = 10.7SQTTQPQRR149 pKa = 11.84TIGVTDD155 pKa = 4.17GSNFQEE161 pKa = 4.47IDD163 pKa = 4.05LPDD166 pKa = 5.32LDD168 pKa = 4.9ILPEE172 pKa = 4.08TLFITINGNQYY183 pKa = 11.53DD184 pKa = 4.24LVDD187 pKa = 4.09SFAEE191 pKa = 4.11SASTDD196 pKa = 2.89RR197 pKa = 11.84HH198 pKa = 4.06FRR200 pKa = 11.84IYY202 pKa = 10.36FRR204 pKa = 11.84SDD206 pKa = 2.53GSSYY210 pKa = 10.52IQLPGVDD217 pKa = 3.55QVDD220 pKa = 3.62NTSFGQVPPQGQTVYY235 pKa = 11.36ANYY238 pKa = 8.89ATGGGANTNVSANTITEE255 pKa = 4.07YY256 pKa = 11.1LGTDD260 pKa = 3.5SNIVSVNNGSPASGGLDD277 pKa = 3.08EE278 pKa = 5.06EE279 pKa = 5.1TIQNAVTIAPLRR291 pKa = 11.84ARR293 pKa = 11.84TTRR296 pKa = 11.84YY297 pKa = 9.4FINRR301 pKa = 11.84STGVAVAKK309 pKa = 10.46EE310 pKa = 3.96IDD312 pKa = 3.48GVLYY316 pKa = 10.93ADD318 pKa = 3.58IVKK321 pKa = 10.55VGALSCEE328 pKa = 4.68CYY330 pKa = 10.17IIPVGGGIASSQLLQEE346 pKa = 4.42VEE348 pKa = 4.28DD349 pKa = 4.36LLVSRR354 pKa = 11.84SPLEE358 pKa = 4.23EE359 pKa = 4.25IEE361 pKa = 4.39VNAYY365 pKa = 5.93TASYY369 pKa = 8.49LTTFVNMQIKK379 pKa = 9.97LLPGFSYY386 pKa = 11.57ADD388 pKa = 3.32VAKK391 pKa = 10.29FSSLAVCQTLHH402 pKa = 6.86VLGQYY407 pKa = 9.91IFEE410 pKa = 4.49VYY412 pKa = 10.48ASNGVQSAIEE422 pKa = 4.65EE423 pKa = 4.29INSIYY428 pKa = 10.78GPIIGYY434 pKa = 7.21TFTIADD440 pKa = 3.69SSGIIQILGNVEE452 pKa = 3.85TAVVSKK458 pKa = 9.84PRR460 pKa = 11.84KK461 pKa = 8.39ISEE464 pKa = 3.77IYY466 pKa = 8.3MALGFVQGLDD476 pKa = 3.45YY477 pKa = 11.62ANIVAPNSDD486 pKa = 3.74LVPNNGSITNVSTVTLTQVNN506 pKa = 3.76

Molecular weight: 54.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343LEH1|A0A343LEH1_9CAUD Uncharacterized protein OS=Leptospira phage LE4 OX=2041383 PE=4 SV=1
MM1 pKa = 8.45IEE3 pKa = 3.75MPGYY7 pKa = 10.3IFILMMMIFWSLGYY21 pKa = 11.29LMGKK25 pKa = 9.82RR26 pKa = 11.84SAKK29 pKa = 9.57KK30 pKa = 10.12RR31 pKa = 11.84PAVHH35 pKa = 6.27NKK37 pKa = 9.92SSVRR41 pKa = 3.54

Molecular weight: 4.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

81

0

81

15527

41

592

191.7

21.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.255 ± 0.285

0.915 ± 0.116

5.326 ± 0.216

8.141 ± 0.507

4.772 ± 0.229

6.157 ± 0.423

1.591 ± 0.152

7.548 ± 0.301

9.719 ± 0.573

7.703 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.312 ± 0.191

5.32 ± 0.18

3.703 ± 0.176

4.115 ± 0.185

4.103 ± 0.149

6.994 ± 0.275

5.706 ± 0.346

5.874 ± 0.263

1.069 ± 0.093

3.677 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski