Desulfovibrio sp. OH1186_COT-070
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2373 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P2A4F8|A0A3P2A4F8_9DELT Uncharacterized protein OS=Desulfovibrio sp. OH1186_COT-070 OX=2491045 GN=EII23_00570 PE=4 SV=1
MM1 pKa = 7.12 QNKK4 pKa = 9.61 EE5 pKa = 4.13 YY6 pKa = 10.9 LHH8 pKa = 6.66 AAAKK12 pKa = 10.47 AIAQQIEE19 pKa = 4.4 GNLLVIGVSLDD30 pKa = 3.56 PDD32 pKa = 3.45 VADD35 pKa = 3.29 YY36 pKa = 10.32 MGAFEE41 pKa = 5.04 EE42 pKa = 4.49 EE43 pKa = 4.98 AITLADD49 pKa = 3.69 VVEE52 pKa = 5.26 DD53 pKa = 3.94 GLLTVTNSGEE63 pKa = 3.92 VMYY66 pKa = 10.99 VGNNN70 pKa = 3.11
Molecular weight: 7.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.786
IPC2_protein 3.834
IPC_protein 3.694
Toseland 3.528
ProMoST 3.872
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.452
Solomon 3.643
Lehninger 3.605
Nozaki 3.821
DTASelect 3.948
Thurlkill 3.592
EMBOSS 3.63
Sillero 3.821
Patrickios 1.837
IPC_peptide 3.643
IPC2_peptide 3.795
IPC2.peptide.svr19 3.751
Protein with the highest isoelectric point:
>tr|A0A3P1ZXK6|A0A3P1ZXK6_9DELT Recombinase family protein OS=Desulfovibrio sp. OH1186_COT-070 OX=2491045 GN=EII23_03890 PE=4 SV=1
MM1 pKa = 7.76 RR2 pKa = 11.84 HH3 pKa = 6.22 PPLPAPLLWQSLLGFWILGILAVAWPVPSASCAFLLFLADD43 pKa = 3.36 ARR45 pKa = 11.84 LWRR48 pKa = 11.84 AKK50 pKa = 10.77 GIFAALAVLLAGVLAGHH67 pKa = 7.22 LTTGKK72 pKa = 8.18 MWRR75 pKa = 11.84 DD76 pKa = 3.44 HH77 pKa = 6.32 TPTPVAWLDD86 pKa = 3.62 TAPKK90 pKa = 10.3 SMRR93 pKa = 11.84 ICGQIQEE100 pKa = 4.39 VRR102 pKa = 11.84 GLPDD106 pKa = 2.93 NRR108 pKa = 11.84 IRR110 pKa = 11.84 MLLADD115 pKa = 4.02 VRR117 pKa = 11.84 PHH119 pKa = 6.47 GGAEE123 pKa = 3.96 KK124 pKa = 10.52 SLSGLMEE131 pKa = 4.11 WTWQNPSAYY140 pKa = 8.68 PLPGQSACLSRR151 pKa = 11.84 RR152 pKa = 11.84 PQAMQGFANPGLPDD166 pKa = 4.01 RR167 pKa = 11.84 DD168 pKa = 3.52 VVIQSVRR175 pKa = 11.84 NVRR178 pKa = 11.84 WRR180 pKa = 11.84 IWSQGEE186 pKa = 4.12 SGQPRR191 pKa = 11.84 IDD193 pKa = 3.75 GQPSLSARR201 pKa = 11.84 WRR203 pKa = 11.84 EE204 pKa = 3.97 SLRR207 pKa = 11.84 QGFFTVLSPDD217 pKa = 3.5 AEE219 pKa = 4.16 HH220 pKa = 7.26 FPRR223 pKa = 11.84 QKK225 pKa = 10.64 LPQGKK230 pKa = 9.82 AILVALLFGDD240 pKa = 4.4 RR241 pKa = 11.84 QFLDD245 pKa = 3.36 NQTIAGFSMAALGHH259 pKa = 5.87 SLALSGQHH267 pKa = 6.35 LAVAGLAGILLVVVVARR284 pKa = 11.84 AYY286 pKa = 9.86 PGIYY290 pKa = 9.79 LCRR293 pKa = 11.84 TRR295 pKa = 11.84 TVWVMSASVPPALAYY310 pKa = 10.37 LWLGNAPPSLVRR322 pKa = 11.84 ATIMLCILALWLLRR336 pKa = 11.84 RR337 pKa = 11.84 RR338 pKa = 11.84 VHH340 pKa = 5.12 TTLDD344 pKa = 3.73 VLCSALFCITLFSPAAVFDD363 pKa = 3.81 TGLQLSAICVAVIGMSMPWLRR384 pKa = 11.84 RR385 pKa = 11.84 LLPRR389 pKa = 11.84 PHH391 pKa = 6.91 PAEE394 pKa = 3.99 HH395 pKa = 6.4 AARR398 pKa = 11.84 WRR400 pKa = 11.84 SLLQDD405 pKa = 4.43 ALWSLQRR412 pKa = 11.84 IFALSLIIQTVLLPVGILLFGNTGHH437 pKa = 7.51 WFPLNVLWLPLVNLLVLPGAALGLLAGACGWDD469 pKa = 3.18 AAARR473 pKa = 11.84 AILEE477 pKa = 4.34 YY478 pKa = 10.93 ASLPCQWLADD488 pKa = 3.83 ALAWLARR495 pKa = 11.84 QNALEE500 pKa = 4.23 SPVVLRR506 pKa = 11.84 PHH508 pKa = 6.04 WTALPAFAALTAAAALRR525 pKa = 11.84 GGRR528 pKa = 11.84 LRR530 pKa = 11.84 LPPAGRR536 pKa = 11.84 RR537 pKa = 11.84 LLLAGALLLCAGPLLRR553 pKa = 11.84 AGEE556 pKa = 4.13 RR557 pKa = 11.84 LSPEE561 pKa = 3.61 VRR563 pKa = 11.84 LDD565 pKa = 3.34 ILDD568 pKa = 3.94 VGQSQALLLRR578 pKa = 11.84 LPGHH582 pKa = 6.05 VRR584 pKa = 11.84 LLLDD588 pKa = 4.0 GSGSASPRR596 pKa = 11.84 FDD598 pKa = 3.44 PGKK601 pKa = 10.62 SLVAPVLVHH610 pKa = 6.7 NDD612 pKa = 3.29 APRR615 pKa = 11.84 LTAVLNSHH623 pKa = 7.66 PDD625 pKa = 3.56 LDD627 pKa = 4.14 HH628 pKa = 6.79 MGGLLYY634 pKa = 10.25 ILRR637 pKa = 11.84 HH638 pKa = 5.71 FRR640 pKa = 11.84 TQGLFDD646 pKa = 3.64 NGKK649 pKa = 8.32 NAKK652 pKa = 9.63 GKK654 pKa = 9.17 WGEE657 pKa = 3.89 LWDD660 pKa = 3.89 LTRR663 pKa = 11.84 QKK665 pKa = 10.61 HH666 pKa = 4.57 AARR669 pKa = 11.84 PLRR672 pKa = 11.84 QGDD675 pKa = 3.9 TLVLGEE681 pKa = 4.7 PEE683 pKa = 3.7 NGLRR687 pKa = 11.84 LEE689 pKa = 4.17 ILHH692 pKa = 6.61 PPRR695 pKa = 11.84 HH696 pKa = 6.29 SEE698 pKa = 4.56 DD699 pKa = 2.58 KK700 pKa = 10.36 WRR702 pKa = 11.84 GNNASLVARR711 pKa = 11.84 LLHH714 pKa = 6.22 HH715 pKa = 6.45 EE716 pKa = 4.39 RR717 pKa = 11.84 GLALFMGDD725 pKa = 3.21 AEE727 pKa = 4.39 RR728 pKa = 11.84 PVLQRR733 pKa = 11.84 LLAEE737 pKa = 4.52 GLDD740 pKa = 3.62 VRR742 pKa = 11.84 AEE744 pKa = 4.0 VLIAPHH750 pKa = 6.55 HH751 pKa = 5.82 GSYY754 pKa = 10.03 TGFLPQFFQAVRR766 pKa = 11.84 PQLVVASCGSRR777 pKa = 11.84 YY778 pKa = 10.02 AYY780 pKa = 9.33 PDD782 pKa = 2.93 KK783 pKa = 10.88 KK784 pKa = 10.53 LRR786 pKa = 11.84 EE787 pKa = 4.17 WLAEE791 pKa = 3.88 NHH793 pKa = 6.22 ISLAYY798 pKa = 8.4 TSRR801 pKa = 11.84 DD802 pKa = 3.14 GAVRR806 pKa = 11.84 IAWQRR811 pKa = 11.84 AGQGFSLPRR820 pKa = 11.84 ISTARR825 pKa = 11.84 KK826 pKa = 8.65 QKK828 pKa = 10.78
Molecular weight: 90.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.458
IPC_protein 10.365
Toseland 10.54
ProMoST 10.335
Dawson 10.643
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.701
Grimsley 10.701
Solomon 10.789
Lehninger 10.745
Nozaki 10.57
DTASelect 10.394
Thurlkill 10.555
EMBOSS 10.95
Sillero 10.584
Patrickios 10.365
IPC_peptide 10.789
IPC2_peptide 9.692
IPC2.peptide.svr19 8.633
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2373
0
2373
758626
26
3022
319.7
35.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.204 ± 0.069
1.657 ± 0.026
5.071 ± 0.037
5.967 ± 0.049
3.827 ± 0.031
8.152 ± 0.048
2.337 ± 0.027
4.431 ± 0.046
3.862 ± 0.042
11.147 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.658 ± 0.027
2.853 ± 0.036
5.245 ± 0.045
3.463 ± 0.027
7.046 ± 0.054
5.592 ± 0.031
4.752 ± 0.049
7.033 ± 0.043
1.317 ± 0.02
2.389 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here