Amino acid dipepetide frequency for Microbacterium phage ChickenKing

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.603AlaAla: 9.603 ± 1.1
0.476AlaCys: 0.476 ± 0.171
5.556AlaAsp: 5.556 ± 0.495
6.27AlaGlu: 6.27 ± 0.633
2.619AlaPhe: 2.619 ± 0.545
8.492AlaGly: 8.492 ± 1.048
2.619AlaHis: 2.619 ± 0.478
4.524AlaIle: 4.524 ± 0.696
6.111AlaLys: 6.111 ± 1.143
10.317AlaLeu: 10.317 ± 1.172
2.46AlaMet: 2.46 ± 0.482
2.857AlaAsn: 2.857 ± 0.643
3.571AlaPro: 3.571 ± 0.464
4.206AlaGln: 4.206 ± 0.604
5.635AlaArg: 5.635 ± 0.633
5.159AlaSer: 5.159 ± 0.712
6.746AlaThr: 6.746 ± 0.724
8.571AlaVal: 8.571 ± 0.836
1.667AlaTrp: 1.667 ± 0.431
2.698AlaTyr: 2.698 ± 0.519
0.0AlaXaa: 0.0 ± 0.0
Cys
0.794CysAla: 0.794 ± 0.251
0.0CysCys: 0.0 ± 0.0
0.397CysAsp: 0.397 ± 0.171
0.159CysGlu: 0.159 ± 0.111
0.238CysPhe: 0.238 ± 0.152
0.794CysGly: 0.794 ± 0.312
0.079CysHis: 0.079 ± 0.084
0.238CysIle: 0.238 ± 0.155
0.476CysLys: 0.476 ± 0.172
0.476CysLeu: 0.476 ± 0.283
0.0CysMet: 0.0 ± 0.0
0.238CysAsn: 0.238 ± 0.142
0.476CysPro: 0.476 ± 0.193
0.159CysGln: 0.159 ± 0.101
0.317CysArg: 0.317 ± 0.152
0.317CysSer: 0.317 ± 0.157
0.556CysThr: 0.556 ± 0.222
0.397CysVal: 0.397 ± 0.146
0.238CysTrp: 0.238 ± 0.12
0.159CysTyr: 0.159 ± 0.119
0.0CysXaa: 0.0 ± 0.0
Asp
5.556AspAla: 5.556 ± 0.705
0.714AspCys: 0.714 ± 0.222
5.0AspAsp: 5.0 ± 0.96
5.397AspGlu: 5.397 ± 1.624
2.54AspPhe: 2.54 ± 0.453
3.651AspGly: 3.651 ± 0.539
1.587AspHis: 1.587 ± 0.326
2.778AspIle: 2.778 ± 0.332
2.698AspLys: 2.698 ± 0.425
5.873AspLeu: 5.873 ± 0.61
1.746AspMet: 1.746 ± 0.391
1.825AspAsn: 1.825 ± 0.407
4.206AspPro: 4.206 ± 0.594
2.857AspGln: 2.857 ± 0.553
3.413AspArg: 3.413 ± 0.63
2.778AspSer: 2.778 ± 0.424
3.413AspThr: 3.413 ± 0.517
4.127AspVal: 4.127 ± 0.548
1.508AspTrp: 1.508 ± 0.351
3.016AspTyr: 3.016 ± 0.362
0.0AspXaa: 0.0 ± 0.0
Glu
8.175GluAla: 8.175 ± 1.02
0.476GluCys: 0.476 ± 0.291
6.429GluAsp: 6.429 ± 1.469
4.524GluGlu: 4.524 ± 0.944
1.825GluPhe: 1.825 ± 0.369
4.603GluGly: 4.603 ± 0.693
1.27GluHis: 1.27 ± 0.385
2.46GluIle: 2.46 ± 0.446
2.381GluLys: 2.381 ± 0.566
5.873GluLeu: 5.873 ± 0.752
1.905GluMet: 1.905 ± 0.425
2.381GluAsn: 2.381 ± 0.414
2.222GluPro: 2.222 ± 0.429
2.54GluGln: 2.54 ± 0.433
4.206GluArg: 4.206 ± 0.712
2.857GluSer: 2.857 ± 0.54
3.651GluThr: 3.651 ± 0.454
4.286GluVal: 4.286 ± 0.539
1.349GluTrp: 1.349 ± 0.349
1.429GluTyr: 1.429 ± 0.373
0.0GluXaa: 0.0 ± 0.0
Phe
2.54PheAla: 2.54 ± 0.464
0.079PheCys: 0.079 ± 0.077
2.063PheAsp: 2.063 ± 0.378
1.905PheGlu: 1.905 ± 0.425
1.19PhePhe: 1.19 ± 0.296
2.698PheGly: 2.698 ± 0.409
0.873PheHis: 0.873 ± 0.288
1.032PheIle: 1.032 ± 0.272
1.587PheLys: 1.587 ± 0.317
1.984PheLeu: 1.984 ± 0.425
0.714PheMet: 0.714 ± 0.299
1.349PheAsn: 1.349 ± 0.311
1.19PhePro: 1.19 ± 0.342
1.508PheGln: 1.508 ± 0.275
2.46PheArg: 2.46 ± 0.382
2.222PheSer: 2.222 ± 0.343
1.825PheThr: 1.825 ± 0.345
1.825PheVal: 1.825 ± 0.362
0.476PheTrp: 0.476 ± 0.238
0.714PheTyr: 0.714 ± 0.35
0.0PheXaa: 0.0 ± 0.0
Gly
6.19GlyAla: 6.19 ± 1.134
0.714GlyCys: 0.714 ± 0.204
5.317GlyAsp: 5.317 ± 0.599
4.444GlyGlu: 4.444 ± 0.783
3.413GlyPhe: 3.413 ± 0.534
5.714GlyGly: 5.714 ± 1.113
1.349GlyHis: 1.349 ± 0.29
5.0GlyIle: 5.0 ± 0.747
4.524GlyLys: 4.524 ± 0.688
6.111GlyLeu: 6.111 ± 0.98
1.984GlyMet: 1.984 ± 0.346
2.778GlyAsn: 2.778 ± 0.447
2.46GlyPro: 2.46 ± 0.476
4.683GlyGln: 4.683 ± 0.731
4.762GlyArg: 4.762 ± 0.584
5.079GlySer: 5.079 ± 0.733
6.587GlyThr: 6.587 ± 0.77
5.873GlyVal: 5.873 ± 0.701
1.508GlyTrp: 1.508 ± 0.286
1.905GlyTyr: 1.905 ± 0.453
0.0GlyXaa: 0.0 ± 0.0
His
1.349HisAla: 1.349 ± 0.396
0.0HisCys: 0.0 ± 0.0
1.19HisAsp: 1.19 ± 0.314
1.032HisGlu: 1.032 ± 0.382
0.556HisPhe: 0.556 ± 0.214
2.143HisGly: 2.143 ± 0.445
0.556HisHis: 0.556 ± 0.214
1.508HisIle: 1.508 ± 0.373
0.952HisLys: 0.952 ± 0.314
1.508HisLeu: 1.508 ± 0.317
0.238HisMet: 0.238 ± 0.153
0.714HisAsn: 0.714 ± 0.258
1.111HisPro: 1.111 ± 0.265
0.556HisGln: 0.556 ± 0.188
0.794HisArg: 0.794 ± 0.254
0.794HisSer: 0.794 ± 0.237
0.952HisThr: 0.952 ± 0.269
1.429HisVal: 1.429 ± 0.336
0.635HisTrp: 0.635 ± 0.204
0.714HisTyr: 0.714 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
4.762IleAla: 4.762 ± 0.491
0.317IleCys: 0.317 ± 0.16
4.762IleAsp: 4.762 ± 0.519
3.571IleGlu: 3.571 ± 0.619
0.873IlePhe: 0.873 ± 0.264
3.889IleGly: 3.889 ± 1.097
0.714IleHis: 0.714 ± 0.218
2.857IleIle: 2.857 ± 0.604
1.825IleLys: 1.825 ± 0.428
2.54IleLeu: 2.54 ± 0.426
1.429IleMet: 1.429 ± 0.305
2.381IleAsn: 2.381 ± 0.537
2.222IlePro: 2.222 ± 0.549
1.746IleGln: 1.746 ± 0.405
2.54IleArg: 2.54 ± 0.52
2.46IleSer: 2.46 ± 0.461
4.048IleThr: 4.048 ± 0.939
3.016IleVal: 3.016 ± 0.644
0.952IleTrp: 0.952 ± 0.246
1.667IleTyr: 1.667 ± 0.325
0.0IleXaa: 0.0 ± 0.0
Lys
5.714LysAla: 5.714 ± 0.986
0.079LysCys: 0.079 ± 0.072
2.54LysAsp: 2.54 ± 0.473
3.095LysGlu: 3.095 ± 0.542
1.429LysPhe: 1.429 ± 0.277
3.968LysGly: 3.968 ± 0.596
0.476LysHis: 0.476 ± 0.191
2.143LysIle: 2.143 ± 0.383
3.413LysLys: 3.413 ± 0.818
4.127LysLeu: 4.127 ± 0.664
1.032LysMet: 1.032 ± 0.287
1.825LysAsn: 1.825 ± 0.326
3.175LysPro: 3.175 ± 0.639
1.587LysGln: 1.587 ± 0.433
2.698LysArg: 2.698 ± 0.541
2.302LysSer: 2.302 ± 0.425
3.413LysThr: 3.413 ± 0.501
4.206LysVal: 4.206 ± 0.574
0.952LysTrp: 0.952 ± 0.291
0.635LysTyr: 0.635 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
8.968LeuAla: 8.968 ± 0.937
0.556LeuCys: 0.556 ± 0.219
4.921LeuAsp: 4.921 ± 0.6
4.921LeuGlu: 4.921 ± 0.616
2.698LeuPhe: 2.698 ± 0.409
6.667LeuGly: 6.667 ± 0.695
1.667LeuHis: 1.667 ± 0.424
5.079LeuIle: 5.079 ± 1.183
3.492LeuLys: 3.492 ± 0.598
7.54LeuLeu: 7.54 ± 0.802
1.746LeuMet: 1.746 ± 0.344
3.333LeuAsn: 3.333 ± 0.55
4.762LeuPro: 4.762 ± 0.714
2.46LeuGln: 2.46 ± 0.446
4.762LeuArg: 4.762 ± 0.833
5.635LeuSer: 5.635 ± 0.687
5.397LeuThr: 5.397 ± 0.572
6.825LeuVal: 6.825 ± 0.744
1.349LeuTrp: 1.349 ± 0.27
1.825LeuTyr: 1.825 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
3.651MetAla: 3.651 ± 0.49
0.159MetCys: 0.159 ± 0.122
1.349MetAsp: 1.349 ± 0.369
1.27MetGlu: 1.27 ± 0.301
0.635MetPhe: 0.635 ± 0.171
1.825MetGly: 1.825 ± 0.395
0.397MetHis: 0.397 ± 0.161
0.873MetIle: 0.873 ± 0.269
0.476MetLys: 0.476 ± 0.168
2.302MetLeu: 2.302 ± 0.482
0.556MetMet: 0.556 ± 0.143
1.032MetAsn: 1.032 ± 0.227
0.873MetPro: 0.873 ± 0.307
1.27MetGln: 1.27 ± 0.328
1.349MetArg: 1.349 ± 0.296
2.381MetSer: 2.381 ± 0.376
2.063MetThr: 2.063 ± 0.394
1.27MetVal: 1.27 ± 0.27
0.317MetTrp: 0.317 ± 0.156
0.476MetTyr: 0.476 ± 0.231
0.0MetXaa: 0.0 ± 0.0
Asn
3.81AsnAla: 3.81 ± 0.514
0.079AsnCys: 0.079 ± 0.093
2.302AsnAsp: 2.302 ± 0.487
2.46AsnGlu: 2.46 ± 0.396
0.397AsnPhe: 0.397 ± 0.186
3.81AsnGly: 3.81 ± 0.488
0.397AsnHis: 0.397 ± 0.172
1.984AsnIle: 1.984 ± 0.462
1.825AsnLys: 1.825 ± 0.448
2.857AsnLeu: 2.857 ± 0.52
0.873AsnMet: 0.873 ± 0.286
1.27AsnAsn: 1.27 ± 0.275
2.063AsnPro: 2.063 ± 0.406
1.508AsnGln: 1.508 ± 0.354
1.349AsnArg: 1.349 ± 0.323
2.46AsnSer: 2.46 ± 0.445
1.825AsnThr: 1.825 ± 0.406
1.984AsnVal: 1.984 ± 0.3
0.635AsnTrp: 0.635 ± 0.229
1.429AsnTyr: 1.429 ± 0.357
0.0AsnXaa: 0.0 ± 0.0
Pro
5.159ProAla: 5.159 ± 0.602
0.159ProCys: 0.159 ± 0.121
2.381ProAsp: 2.381 ± 0.395
3.333ProGlu: 3.333 ± 0.646
1.111ProPhe: 1.111 ± 0.296
3.968ProGly: 3.968 ± 0.724
0.397ProHis: 0.397 ± 0.148
1.905ProIle: 1.905 ± 0.292
1.825ProLys: 1.825 ± 0.387
3.651ProLeu: 3.651 ± 0.561
0.635ProMet: 0.635 ± 0.198
1.905ProAsn: 1.905 ± 0.443
1.19ProPro: 1.19 ± 0.358
1.825ProGln: 1.825 ± 0.38
2.619ProArg: 2.619 ± 0.648
3.016ProSer: 3.016 ± 0.511
4.127ProThr: 4.127 ± 0.767
3.889ProVal: 3.889 ± 0.514
1.111ProTrp: 1.111 ± 0.269
1.19ProTyr: 1.19 ± 0.26
0.0ProXaa: 0.0 ± 0.0
Gln
4.762GlnAla: 4.762 ± 0.566
0.159GlnCys: 0.159 ± 0.117
2.063GlnAsp: 2.063 ± 0.389
3.81GlnGlu: 3.81 ± 0.624
0.556GlnPhe: 0.556 ± 0.213
2.778GlnGly: 2.778 ± 0.436
0.714GlnHis: 0.714 ± 0.224
1.825GlnIle: 1.825 ± 0.363
2.302GlnLys: 2.302 ± 0.499
3.095GlnLeu: 3.095 ± 0.458
1.19GlnMet: 1.19 ± 0.298
1.667GlnAsn: 1.667 ± 0.49
1.587GlnPro: 1.587 ± 0.315
1.19GlnGln: 1.19 ± 0.305
2.222GlnArg: 2.222 ± 0.388
2.222GlnSer: 2.222 ± 0.372
2.063GlnThr: 2.063 ± 0.376
2.857GlnVal: 2.857 ± 0.552
0.952GlnTrp: 0.952 ± 0.302
1.508GlnTyr: 1.508 ± 0.313
0.0GlnXaa: 0.0 ± 0.0
Arg
5.556ArgAla: 5.556 ± 0.689
0.794ArgCys: 0.794 ± 0.311
3.413ArgAsp: 3.413 ± 0.565
3.968ArgGlu: 3.968 ± 0.47
1.984ArgPhe: 1.984 ± 0.417
3.651ArgGly: 3.651 ± 0.541
0.952ArgHis: 0.952 ± 0.361
2.778ArgIle: 2.778 ± 0.417
3.413ArgLys: 3.413 ± 0.723
5.952ArgLeu: 5.952 ± 0.911
2.143ArgMet: 2.143 ± 0.401
2.222ArgAsn: 2.222 ± 0.359
1.905ArgPro: 1.905 ± 0.367
1.984ArgGln: 1.984 ± 0.411
3.095ArgArg: 3.095 ± 0.608
3.095ArgSer: 3.095 ± 0.499
3.333ArgThr: 3.333 ± 0.637
4.524ArgVal: 4.524 ± 0.582
1.349ArgTrp: 1.349 ± 0.314
1.429ArgTyr: 1.429 ± 0.349
0.0ArgXaa: 0.0 ± 0.0
Ser
5.317SerAla: 5.317 ± 0.648
0.317SerCys: 0.317 ± 0.178
3.73SerAsp: 3.73 ± 0.615
3.492SerGlu: 3.492 ± 0.631
2.46SerPhe: 2.46 ± 0.427
5.714SerGly: 5.714 ± 0.968
1.111SerHis: 1.111 ± 0.3
2.302SerIle: 2.302 ± 0.486
3.571SerLys: 3.571 ± 0.548
4.841SerLeu: 4.841 ± 0.694
1.508SerMet: 1.508 ± 0.417
1.667SerAsn: 1.667 ± 0.392
2.54SerPro: 2.54 ± 0.432
2.143SerGln: 2.143 ± 0.413
3.095SerArg: 3.095 ± 0.57
3.968SerSer: 3.968 ± 0.499
3.968SerThr: 3.968 ± 0.582
4.365SerVal: 4.365 ± 0.581
0.794SerTrp: 0.794 ± 0.246
2.381SerTyr: 2.381 ± 0.459
0.0SerXaa: 0.0 ± 0.0
Thr
5.952ThrAla: 5.952 ± 0.96
0.238ThrCys: 0.238 ± 0.154
3.651ThrAsp: 3.651 ± 0.55
3.016ThrGlu: 3.016 ± 0.568
2.54ThrPhe: 2.54 ± 0.354
6.349ThrGly: 6.349 ± 0.644
0.873ThrHis: 0.873 ± 0.263
3.81ThrIle: 3.81 ± 0.486
2.381ThrLys: 2.381 ± 0.474
5.238ThrLeu: 5.238 ± 0.669
1.746ThrMet: 1.746 ± 0.336
1.746ThrAsn: 1.746 ± 0.262
3.73ThrPro: 3.73 ± 0.486
2.302ThrGln: 2.302 ± 0.495
4.365ThrArg: 4.365 ± 0.537
4.206ThrSer: 4.206 ± 0.521
5.079ThrThr: 5.079 ± 0.699
6.111ThrVal: 6.111 ± 0.742
1.111ThrTrp: 1.111 ± 0.264
2.54ThrTyr: 2.54 ± 0.391
0.0ThrXaa: 0.0 ± 0.0
Val
8.175ValAla: 8.175 ± 0.658
0.476ValCys: 0.476 ± 0.179
4.444ValAsp: 4.444 ± 0.675
5.159ValGlu: 5.159 ± 0.627
1.746ValPhe: 1.746 ± 0.421
5.397ValGly: 5.397 ± 0.844
1.746ValHis: 1.746 ± 0.327
3.175ValIle: 3.175 ± 0.619
3.016ValLys: 3.016 ± 0.561
5.794ValLeu: 5.794 ± 0.817
1.825ValMet: 1.825 ± 0.416
2.222ValAsn: 2.222 ± 0.419
3.333ValPro: 3.333 ± 0.543
4.048ValGln: 4.048 ± 0.44
5.0ValArg: 5.0 ± 0.697
4.444ValSer: 4.444 ± 0.579
4.841ValThr: 4.841 ± 0.652
5.397ValVal: 5.397 ± 0.898
1.746ValTrp: 1.746 ± 0.347
2.302ValTyr: 2.302 ± 0.394
0.0ValXaa: 0.0 ± 0.0
Trp
0.952TrpAla: 0.952 ± 0.281
0.159TrpCys: 0.159 ± 0.123
1.19TrpAsp: 1.19 ± 0.304
1.349TrpGlu: 1.349 ± 0.373
0.873TrpPhe: 0.873 ± 0.361
1.27TrpGly: 1.27 ± 0.372
0.635TrpHis: 0.635 ± 0.228
1.111TrpIle: 1.111 ± 0.236
1.111TrpLys: 1.111 ± 0.306
2.302TrpLeu: 2.302 ± 0.448
0.159TrpMet: 0.159 ± 0.106
0.714TrpAsn: 0.714 ± 0.209
0.952TrpPro: 0.952 ± 0.259
0.556TrpGln: 0.556 ± 0.183
0.794TrpArg: 0.794 ± 0.216
1.27TrpSer: 1.27 ± 0.338
1.746TrpThr: 1.746 ± 0.362
1.667TrpVal: 1.667 ± 0.343
0.714TrpTrp: 0.714 ± 0.287
0.635TrpTyr: 0.635 ± 0.192
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.175TyrAla: 3.175 ± 0.405
0.556TyrCys: 0.556 ± 0.188
1.984TyrAsp: 1.984 ± 0.356
1.667TyrGlu: 1.667 ± 0.369
0.556TyrPhe: 0.556 ± 0.174
3.016TyrGly: 3.016 ± 0.519
0.317TyrHis: 0.317 ± 0.192
1.19TyrIle: 1.19 ± 0.309
1.429TyrLys: 1.429 ± 0.365
2.222TyrLeu: 2.222 ± 0.369
0.556TyrMet: 0.556 ± 0.234
1.19TyrAsn: 1.19 ± 0.339
1.825TyrPro: 1.825 ± 0.531
0.397TyrGln: 0.397 ± 0.166
2.063TyrArg: 2.063 ± 0.463
2.619TyrSer: 2.619 ± 0.46
1.429TyrThr: 1.429 ± 0.331
1.667TyrVal: 1.667 ± 0.34
0.794TyrTrp: 0.794 ± 0.213
0.794TyrTyr: 0.794 ± 0.321
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (12601 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski