Lactobacillus phage phiAT3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fattrevirus; Lactobacillus virus phiAT3

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6J1Y2|Q6J1Y2_9CAUD Putative major head protein OS=Lactobacillus phage phiAT3 OX=279281 PE=4 SV=1
MM1 pKa = 7.78ADD3 pKa = 3.28EE4 pKa = 4.42TADD7 pKa = 3.97TVGVTADD14 pKa = 4.54DD15 pKa = 3.72MQSYY19 pKa = 11.02LNLDD23 pKa = 3.42SDD25 pKa = 4.27GDD27 pKa = 3.85ASILEE32 pKa = 4.3GLISTAEE39 pKa = 4.14SAVMNAIDD47 pKa = 3.69DD48 pKa = 4.52TIAVEE53 pKa = 4.59VYY55 pKa = 8.58RR56 pKa = 11.84TYY58 pKa = 11.3PLFNQAVRR66 pKa = 11.84VLVDD70 pKa = 4.04FMYY73 pKa = 10.7YY74 pKa = 10.32SRR76 pKa = 11.84GTLSDD81 pKa = 3.14QSKK84 pKa = 10.57AYY86 pKa = 8.3PPSYY90 pKa = 10.8AYY92 pKa = 9.53MINSIRR98 pKa = 11.84WKK100 pKa = 9.6IQRR103 pKa = 11.84DD104 pKa = 3.45QAAKK108 pKa = 10.69AGGNDD113 pKa = 3.44GG114 pKa = 4.08

Molecular weight: 12.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6J1T9|Q6J1T9_9CAUD Uncharacterized protein OS=Lactobacillus phage phiAT3 OX=279281 PE=4 SV=1
MM1 pKa = 7.5PKK3 pKa = 10.02VRR5 pKa = 11.84IPRR8 pKa = 11.84HH9 pKa = 4.84RR10 pKa = 11.84VFPPSHH16 pKa = 6.87GDD18 pKa = 3.28DD19 pKa = 3.24KK20 pKa = 11.27TVARR24 pKa = 11.84RR25 pKa = 11.84GKK27 pKa = 7.82WTGCDD32 pKa = 3.39SQAGSIPACHH42 pKa = 6.28IVQFSDD48 pKa = 3.15RR49 pKa = 11.84TQLAMTPSDD58 pKa = 3.4TGRR61 pKa = 11.84ASSNRR66 pKa = 11.84SSSAA70 pKa = 2.89

Molecular weight: 7.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11740

50

1299

213.5

23.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.339 ± 0.474

0.716 ± 0.134

7.112 ± 0.388

5.085 ± 0.332

3.68 ± 0.215

6.661 ± 0.473

2.189 ± 0.217

5.86 ± 0.187

7.394 ± 0.399

7.359 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.169

5.801 ± 0.228

3.339 ± 0.267

4.54 ± 0.313

4.446 ± 0.377

6.721 ± 0.315

5.911 ± 0.335

6.457 ± 0.241

1.763 ± 0.226

4.046 ± 0.368
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski