Cavenderia fasciculata (strain SH3) (Slime mold) (Dictyostelium fasciculatum)

Taxonomy: cellular organisms; Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Acytosteliales; Cavenderiaceae; Cavenderia; Cavenderia fasciculata

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12152 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4PX52|F4PX52_CAVFA AAA domain-containing protein OS=Cavenderia fasciculata (strain SH3) OX=1054147 GN=DFA_06958 PE=4 SV=1
MM1 pKa = 7.38GFEE4 pKa = 4.84CIMRR8 pKa = 11.84ASTPCVGTQCCTLSAFCAPPRR29 pKa = 11.84QLAPTLASVSGLVGGSNDD47 pKa = 2.74ITTTATTTATTTCSTTASTMGTTTASTLATTTDD80 pKa = 2.99WQTIGLTVPITTTASTTASTLASTTASTTASTTASTIGVVSASTTATTTATSIGSTTASTASTTASTTATTLASTTASTTATMLVTTTASTTASTIASTTASTTASTTATTTSPQTIGRR199 pKa = 11.84ISTTASTSASTTASTLGTTTASTLASTTASTTASTTASTMGSTTASTTASTSASTTATTTATSTATTTASTTATTASTTASTTASTTATTTASSTATTTASTSASTTASTTATTTASTTASTIASTSASTTASTIGSTSASTTASTTATTTAFITTANPTTNLPFYY365 pKa = 10.87SSTTTSDD372 pKa = 3.05IFDD375 pKa = 4.02SPSASGTTSASGKK388 pKa = 8.54TSSTPTTTGTASASGTASSTPTTTTAPSTASTSASGTVSATGITGTASASGTASSTPTTTAPSTASATGFTGISSGRR465 pKa = 11.84GIVSGSGGGGGGGGGGGGGGGDD487 pKa = 3.8TASSTPTSIDD497 pKa = 2.63ISASPTSIDD506 pKa = 3.51ISSEE510 pKa = 4.06SQTTTGTSSDD520 pKa = 3.54PTTTTGTSSASGTASASGTDD540 pKa = 3.25SSTPTTTTTGTASSTDD556 pKa = 2.95SSTPTTGTATTTGTASSTPTTTGTASSTPTTTGTASSTDD595 pKa = 3.32SSSTTTGTASASGTASASGTATTTGTASATDD626 pKa = 3.41SSTPTTTGTASSTPTTTGTASSTPTTGTASASGTGTASATGITASATGIATGVTASATGIATGVTGTATATGIATGVTGTATGVTGTATGVTASATGIATGVTGTASATGIATGVTGTASANGVTASATGIATGVTASATGIATGVSGTASATGIATGVSGTASATGIATGVTATATGIATGVTGTASATGIATGVTGTASATGIATGVTGTASATGVTGTASATGVTGTASATGITGVTSASATGITGVTSASATGITGTASATGITSASATGVTGTASATGITGVTSASATGITGVTSASATGVTGTASATGVTGTASATGVTGTASATGIATGVTASATGIATGVTASATGVTGTATGVTGTATGVTTTATGIATGVTGTATGIATGVTGTATGVTASATGIATGVTGTATGIATGVTGTATGIATGVTASATGVTASATGIATGVTGTASATGIATGVTGTASATGVTASATGIATGVTGTASATGIATGVTASATGVTGTATGIATGVTASATGVTASATGVTASATGIATGVTGTASATGIATGVTASATGIATGVTASATGIATGVTASATGIATGVTGTASATGIATGVTGTASATGVTASATGIATGVTGTASATGIATGVTGTATGIATGVTASATGVTASATGVTASATGIATGVTASATGVTTTATGIATGVTGTASATGVTASATGIATGVTASATGIATGVTASATGIATGVTGTATGIATGVTASATGIATGVTGTASATGVTGTATGIATGVTASATGIATGVTASATGVTTTATGITGTASATGIATGVTASATGVTGTATGIATGVTASATGIATGVTGTASATGVTGTASATGIATGVTGTASATGIATGVTGTASATGVTGTATGIATGVTASATGIATGVTASATGIATGVTGTASATGIATGVTGTASATGVTGTATGIATGVTGTASATGVTGTATATGVTASATGIATGVTGTASATGVTASATGIATGVTGTATATGVTASATATGVTASATGIATGG1648 pKa = 3.67

Molecular weight: 143.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4PLB3|F4PLB3_CAVFA Uncharacterized protein OS=Cavenderia fasciculata (strain SH3) OX=1054147 GN=DFA_05467 PE=4 SV=1
MM1 pKa = 7.03VLSGAGSGSVLSVSLSGGTRR21 pKa = 11.84KK22 pKa = 9.93PIGSQPISQRR32 pKa = 11.84PFSRR36 pKa = 11.84PPSSSLPTSNRR47 pKa = 11.84KK48 pKa = 8.64

Molecular weight: 4.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12152

0

12152

6765862

16

7796

556.8

62.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.523 ± 0.02

1.497 ± 0.012

5.583 ± 0.017

5.46 ± 0.026

4.205 ± 0.015

4.953 ± 0.021

2.31 ± 0.011

7.252 ± 0.02

6.464 ± 0.027

8.847 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.007

7.196 ± 0.028

4.565 ± 0.022

5.25 ± 0.027

3.621 ± 0.015

9.534 ± 0.029

6.82 ± 0.029

5.432 ± 0.017

0.865 ± 0.005

3.582 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski