Nonomuraea sp. ATCC 55076
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11313 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V0AEX4|A0A1V0AEX4_9ACTN Dehydrogenase OS=Nonomuraea sp. ATCC 55076 OX=1909395 GN=BKM31_51360 PE=4 SV=1
MM1 pKa = 7.48 FEE3 pKa = 4.48 DD4 pKa = 4.41 LIGRR8 pKa = 11.84 VLVCSHH14 pKa = 6.49 ATSAPGTEE22 pKa = 4.65 VPPAPAIDD30 pKa = 3.52 TAEE33 pKa = 3.92 IARR36 pKa = 11.84 YY37 pKa = 9.75 LEE39 pKa = 4.02 SLMNSFSSACTSTGTASTGTSGDD62 pKa = 3.69 DD63 pKa = 3.67 SVDD66 pKa = 3.51 GASEE70 pKa = 4.02 NASGASAGASQSSASLLDD88 pKa = 3.85 VIGVNQLLGTITGYY102 pKa = 10.96 SSACGTPQAAGGTSTSLLDD121 pKa = 4.15 SIGVTNLFKK130 pKa = 11.06 ALTGG134 pKa = 3.71
Molecular weight: 13.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.884
IPC_protein 3.783
Toseland 3.592
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.503
Solomon 3.757
Lehninger 3.706
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.91
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A1V0A865|A0A1V0A865_9ACTN Uncharacterized protein OS=Nonomuraea sp. ATCC 55076 OX=1909395 GN=BKM31_37615 PE=4 SV=1
MM1 pKa = 7.42 TLRR4 pKa = 11.84 LRR6 pKa = 11.84 MPQVHH11 pKa = 6.16 SAPVRR16 pKa = 11.84 RR17 pKa = 11.84 ATLHH21 pKa = 6.37 ARR23 pKa = 11.84 LATEE27 pKa = 4.33 GLALRR32 pKa = 11.84 RR33 pKa = 11.84 LAPTRR38 pKa = 11.84 PRR40 pKa = 11.84 PLPMRR45 pKa = 11.84 RR46 pKa = 11.84 TRR48 pKa = 11.84 MRR50 pKa = 11.84 PRR52 pKa = 11.84 LLPPGRR58 pKa = 11.84 VPLRR62 pKa = 11.84 PGTLHH67 pKa = 5.63 VRR69 pKa = 11.84 SVTARR74 pKa = 11.84 SGRR77 pKa = 11.84 MSFGVLRR84 pKa = 11.84 LAWLLLTALWRR95 pKa = 11.84 AAVRR99 pKa = 11.84 LSTARR104 pKa = 11.84 PRR106 pKa = 11.84 RR107 pKa = 11.84 GRR109 pKa = 11.84 LSGRR113 pKa = 11.84 WW114 pKa = 3.23
Molecular weight: 13.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.603
Grimsley 13.144
Solomon 13.598
Lehninger 13.495
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.31
IPC_peptide 13.598
IPC2_peptide 12.588
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11313
0
11313
3677589
29
4412
325.1
34.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.569 ± 0.03
0.758 ± 0.006
5.687 ± 0.017
5.603 ± 0.025
2.816 ± 0.013
9.406 ± 0.024
2.253 ± 0.011
3.426 ± 0.014
1.969 ± 0.018
10.767 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.851 ± 0.009
1.731 ± 0.013
6.122 ± 0.019
2.718 ± 0.014
8.254 ± 0.024
4.834 ± 0.016
5.866 ± 0.022
8.589 ± 0.021
1.616 ± 0.01
2.166 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here