Nonomuraea sp. ATCC 55076

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Nonomuraea; unclassified Nonomuraea

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11313 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1V0AEX4|A0A1V0AEX4_9ACTN Dehydrogenase OS=Nonomuraea sp. ATCC 55076 OX=1909395 GN=BKM31_51360 PE=4 SV=1
MM1 pKa = 7.48FEE3 pKa = 4.48DD4 pKa = 4.41LIGRR8 pKa = 11.84VLVCSHH14 pKa = 6.49ATSAPGTEE22 pKa = 4.65VPPAPAIDD30 pKa = 3.52TAEE33 pKa = 3.92IARR36 pKa = 11.84YY37 pKa = 9.75LEE39 pKa = 4.02SLMNSFSSACTSTGTASTGTSGDD62 pKa = 3.69DD63 pKa = 3.67SVDD66 pKa = 3.51GASEE70 pKa = 4.02NASGASAGASQSSASLLDD88 pKa = 3.85VIGVNQLLGTITGYY102 pKa = 10.96SSACGTPQAAGGTSTSLLDD121 pKa = 4.15SIGVTNLFKK130 pKa = 11.06ALTGG134 pKa = 3.71

Molecular weight:
13.07 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1V0A865|A0A1V0A865_9ACTN Uncharacterized protein OS=Nonomuraea sp. ATCC 55076 OX=1909395 GN=BKM31_37615 PE=4 SV=1
MM1 pKa = 7.42TLRR4 pKa = 11.84LRR6 pKa = 11.84MPQVHH11 pKa = 6.16SAPVRR16 pKa = 11.84RR17 pKa = 11.84ATLHH21 pKa = 6.37ARR23 pKa = 11.84LATEE27 pKa = 4.33GLALRR32 pKa = 11.84RR33 pKa = 11.84LAPTRR38 pKa = 11.84PRR40 pKa = 11.84PLPMRR45 pKa = 11.84RR46 pKa = 11.84TRR48 pKa = 11.84MRR50 pKa = 11.84PRR52 pKa = 11.84LLPPGRR58 pKa = 11.84VPLRR62 pKa = 11.84PGTLHH67 pKa = 5.63VRR69 pKa = 11.84SVTARR74 pKa = 11.84SGRR77 pKa = 11.84MSFGVLRR84 pKa = 11.84LAWLLLTALWRR95 pKa = 11.84AAVRR99 pKa = 11.84LSTARR104 pKa = 11.84PRR106 pKa = 11.84RR107 pKa = 11.84GRR109 pKa = 11.84LSGRR113 pKa = 11.84WW114 pKa = 3.23

Molecular weight:
13.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11313

0

11313

3677589

29

4412

325.1

34.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.569 ± 0.03

0.758 ± 0.006

5.687 ± 0.017

5.603 ± 0.025

2.816 ± 0.013

9.406 ± 0.024

2.253 ± 0.011

3.426 ± 0.014

1.969 ± 0.018

10.767 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.851 ± 0.009

1.731 ± 0.013

6.122 ± 0.019

2.718 ± 0.014

8.254 ± 0.024

4.834 ± 0.016

5.866 ± 0.022

8.589 ± 0.021

1.616 ± 0.01

2.166 ± 0.013

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski