Moraxella phage Mcat8
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PHP4|A0A0R6PHP4_9CAUD Uncharacterized protein OS=Moraxella phage Mcat8 OX=1647554 PE=4 SV=1
MM1 pKa = 7.97 DD2 pKa = 5.69 FIEE5 pKa = 4.49 LQFSEE10 pKa = 5.12 EE11 pKa = 3.87 EE12 pKa = 3.95 LAQFDD17 pKa = 5.11 DD18 pKa = 6.47 DD19 pKa = 3.87 EE20 pKa = 4.33 QQYY23 pKa = 11.23 YY24 pKa = 11.09 DD25 pKa = 4.18 LLAHH29 pKa = 6.66 EE30 pKa = 5.49 FGLPFEE36 pKa = 5.25 SEE38 pKa = 4.5 AKK40 pKa = 10.52 NEE42 pKa = 4.47 SIPNQQRR49 pKa = 11.84 SS50 pKa = 3.25
Molecular weight: 5.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.134
IPC2_protein 3.77
IPC_protein 3.643
Toseland 3.478
ProMoST 3.808
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.541
Rodwell 3.49
Grimsley 3.401
Solomon 3.579
Lehninger 3.528
Nozaki 3.757
DTASelect 3.859
Thurlkill 3.541
EMBOSS 3.554
Sillero 3.757
Patrickios 3.058
IPC_peptide 3.579
IPC2_peptide 3.732
IPC2.peptide.svr19 3.716
Protein with the highest isoelectric point:
>tr|A0A0R6PHK6|A0A0R6PHK6_9CAUD Uncharacterized protein OS=Moraxella phage Mcat8 OX=1647554 PE=4 SV=1
MM1 pKa = 7.48 CQTICGVGIKK11 pKa = 9.86 PKK13 pKa = 8.94 QLKK16 pKa = 9.46 RR17 pKa = 11.84 LKK19 pKa = 10.12 NRR21 pKa = 11.84 HH22 pKa = 4.44 RR23 pKa = 11.84 QQRR26 pKa = 11.84 LSLFLRR32 pKa = 11.84 QACPKK37 pKa = 10.47 SKK39 pKa = 10.17 GANYY43 pKa = 10.05 EE44 pKa = 4.13 YY45 pKa = 11.38
Molecular weight: 5.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.794
IPC_protein 10.204
Toseland 10.716
ProMoST 10.321
Dawson 10.818
Bjellqvist 10.467
Wikipedia 10.965
Rodwell 11.272
Grimsley 10.847
Solomon 10.877
Lehninger 10.862
Nozaki 10.716
DTASelect 10.452
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.73
Patrickios 11.052
IPC_peptide 10.891
IPC2_peptide 9.589
IPC2.peptide.svr19 8.464
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
12163
39
1750
217.2
24.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.205 ± 0.62
0.937 ± 0.174
6.207 ± 0.217
5.352 ± 0.337
3.905 ± 0.247
6.791 ± 0.313
2.195 ± 0.214
7.062 ± 0.276
7.63 ± 0.502
8.263 ± 0.325
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.302 ± 0.255
5.517 ± 0.332
2.96 ± 0.271
5.262 ± 0.286
4.473 ± 0.247
6.487 ± 0.546
6.207 ± 0.377
5.525 ± 0.246
1.159 ± 0.112
3.56 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here