Torque teno mini virus 10
Average proteome isoelectric point is 7.89
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8DMQ5|A8DMQ5_9VIRU Capsid protein OS=Torque teno mini virus 10 OX=2065036 PE=3 SV=1
MM1 pKa = 7.61 SKK3 pKa = 10.01 QLQNPVYY10 pKa = 9.56 YY11 pKa = 10.51 GKK13 pKa = 10.47 ALEE16 pKa = 4.34 SQWRR20 pKa = 11.84 NNIFNSHH27 pKa = 6.9 DD28 pKa = 3.85 LMCGCKK34 pKa = 10.43 DD35 pKa = 3.59 PVLHH39 pKa = 7.03 LLIILNRR46 pKa = 11.84 EE47 pKa = 3.75 GNAPKK52 pKa = 10.39 PEE54 pKa = 3.96 EE55 pKa = 3.83 DD56 pKa = 3.69 VKK58 pKa = 11.3 NIKK61 pKa = 10.3 CLITGEE67 pKa = 4.21 GAKK70 pKa = 10.63 DD71 pKa = 3.63 GEE73 pKa = 4.36 KK74 pKa = 10.64 DD75 pKa = 3.44 IIPDD79 pKa = 3.59 DD80 pKa = 4.13 FEE82 pKa = 6.61 PGEE85 pKa = 4.22 LDD87 pKa = 3.65 RR88 pKa = 11.84 LFSEE92 pKa = 4.48 LGEE95 pKa = 4.19 TEE97 pKa = 4.83 EE98 pKa = 4.44 EE99 pKa = 4.52 DD100 pKa = 4.94 EE101 pKa = 5.26 NPTDD105 pKa = 4.85 DD106 pKa = 4.02 GG107 pKa = 4.22
Molecular weight: 12.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.398
IPC2_protein 4.291
IPC_protein 4.215
Toseland 4.037
ProMoST 4.329
Dawson 4.164
Bjellqvist 4.317
Wikipedia 4.05
Rodwell 4.05
Grimsley 3.948
Solomon 4.164
Lehninger 4.113
Nozaki 4.279
DTASelect 4.431
Thurlkill 4.062
EMBOSS 4.062
Sillero 4.329
Patrickios 3.77
IPC_peptide 4.164
IPC2_peptide 4.317
IPC2.peptide.svr19 4.245
Protein with the highest isoelectric point:
>tr|A8DMQ7|A8DMQ7_9VIRU ORF4 OS=Torque teno mini virus 10 OX=2065036 PE=4 SV=1
MM1 pKa = 7.47 ILVHH5 pKa = 6.39 SLPGIFPVTSMKK17 pKa = 10.09 EE18 pKa = 3.66 YY19 pKa = 10.34 KK20 pKa = 10.46 YY21 pKa = 10.42 RR22 pKa = 11.84 IQKK25 pKa = 9.49 QNHH28 pKa = 5.25 KK29 pKa = 10.2 QNSKK33 pKa = 10.46 NGTGEE38 pKa = 3.96 GTTLHH43 pKa = 6.68 PKK45 pKa = 10.21 LSKK48 pKa = 10.5 EE49 pKa = 4.04 LVATRR54 pKa = 11.84 QLMKK58 pKa = 9.73 MYY60 pKa = 10.72 KK61 pKa = 10.04 FLQEE65 pKa = 4.06 PKK67 pKa = 9.09 EE68 pKa = 4.32 TCQYY72 pKa = 11.12 ADD74 pKa = 3.16 KK75 pKa = 10.52 HH76 pKa = 5.17 HH77 pKa = 5.93 QRR79 pKa = 11.84 TRR81 pKa = 11.84 ARR83 pKa = 11.84 KK84 pKa = 7.66 RR85 pKa = 11.84 KK86 pKa = 9.09 KK87 pKa = 9.69 RR88 pKa = 11.84 RR89 pKa = 11.84 HH90 pKa = 4.97 HH91 pKa = 6.4 RR92 pKa = 11.84 PRR94 pKa = 11.84 SKK96 pKa = 9.67 STSSNEE102 pKa = 3.9 SNTNSSSEE110 pKa = 4.25 SSSDD114 pKa = 3.14
Molecular weight: 13.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.248
IPC2_protein 9.75
IPC_protein 9.999
Toseland 10.73
ProMoST 10.248
Dawson 10.804
Bjellqvist 10.423
Wikipedia 10.935
Rodwell 11.345
Grimsley 10.847
Solomon 10.862
Lehninger 10.847
Nozaki 10.687
DTASelect 10.423
Thurlkill 10.701
EMBOSS 11.096
Sillero 10.716
Patrickios 11.067
IPC_peptide 10.862
IPC2_peptide 9.004
IPC2.peptide.svr19 8.708
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
988
107
650
247.0
29.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.53 ± 0.554
1.518 ± 0.255
4.858 ± 1.089
6.073 ± 1.639
4.453 ± 0.791
4.858 ± 0.774
2.632 ± 0.711
4.757 ± 0.728
8.502 ± 0.949
6.984 ± 0.65
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.923 ± 0.333
6.579 ± 0.355
7.895 ± 0.867
5.364 ± 0.5
7.287 ± 0.994
5.668 ± 1.601
7.794 ± 1.11
3.441 ± 0.532
2.126 ± 0.682
4.757 ± 1.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here