Staphylococcus phage phiSA_BS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Baoshanvirus; Staphylococcus virus BS1

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1MXQ9|A0A2P1MXQ9_9CAUD Uncharacterized protein OS=Staphylococcus phage phiSA_BS1 OX=2126734 PE=4 SV=1
MM1 pKa = 7.36FMYY4 pKa = 10.37ILIFIVCVGVGIHH17 pKa = 6.74LSRR20 pKa = 11.84KK21 pKa = 9.03IDD23 pKa = 3.8EE24 pKa = 4.05NWEE27 pKa = 3.8NGLYY31 pKa = 10.64DD32 pKa = 3.92HH33 pKa = 7.49LYY35 pKa = 10.57YY36 pKa = 10.84DD37 pKa = 4.65DD38 pKa = 6.44DD39 pKa = 4.66EE40 pKa = 7.18DD41 pKa = 4.02EE42 pKa = 5.2DD43 pKa = 4.97EE44 pKa = 4.84YY45 pKa = 11.8DD46 pKa = 4.3EE47 pKa = 4.88EE48 pKa = 6.45DD49 pKa = 4.68DD50 pKa = 4.6YY51 pKa = 12.13EE52 pKa = 5.6SDD54 pKa = 3.54SDD56 pKa = 4.26SEE58 pKa = 4.52EE59 pKa = 4.03VTSEE63 pKa = 4.23SNSEE67 pKa = 4.08PEE69 pKa = 5.68LKK71 pKa = 10.78DD72 pKa = 5.05LDD74 pKa = 4.37DD75 pKa = 5.61LDD77 pKa = 3.79RR78 pKa = 11.84TKK80 pKa = 11.34

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1MXQ0|A0A2P1MXQ0_9CAUD NUMOD4 domain-containing protein OS=Staphylococcus phage phiSA_BS1 OX=2126734 PE=4 SV=1
MM1 pKa = 7.18TKK3 pKa = 10.34KK4 pKa = 10.44NKK6 pKa = 9.68PFVNYY11 pKa = 10.47APLGNMLGVNNKK23 pKa = 9.26PLFYY27 pKa = 10.3KK28 pKa = 10.53RR29 pKa = 11.84NRR31 pKa = 11.84VEE33 pKa = 4.21IGDD36 pKa = 3.77SAKK39 pKa = 10.01TIKK42 pKa = 10.8YY43 pKa = 9.34KK44 pKa = 10.17IAFVKK49 pKa = 10.28VYY51 pKa = 10.79KK52 pKa = 10.58NVSTKK57 pKa = 10.71DD58 pKa = 3.34LSLQRR63 pKa = 11.84KK64 pKa = 8.01YY65 pKa = 11.37ALNLITDD72 pKa = 4.37FLNIKK77 pKa = 8.61EE78 pKa = 4.07QFLTIRR84 pKa = 11.84QHH86 pKa = 7.03KK87 pKa = 7.83KK88 pKa = 8.76TEE90 pKa = 5.21DD91 pKa = 3.16ILHH94 pKa = 6.63TDD96 pKa = 2.73RR97 pKa = 11.84VYY99 pKa = 11.16YY100 pKa = 9.53IRR102 pKa = 11.84KK103 pKa = 8.99GKK105 pKa = 10.07KK106 pKa = 9.78LLGKK110 pKa = 10.41CSIRR114 pKa = 11.84EE115 pKa = 3.85QRR117 pKa = 11.84TFTQTNLIIIFRR129 pKa = 11.84YY130 pKa = 8.36RR131 pKa = 11.84VRR133 pKa = 11.84VSGRR137 pKa = 11.84KK138 pKa = 8.97ARR140 pKa = 11.84SLTNEE145 pKa = 4.18SKK147 pKa = 10.67DD148 pKa = 3.56QKK150 pKa = 11.03KK151 pKa = 10.15GG152 pKa = 3.38

Molecular weight: 17.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

200

0

200

39824

37

1406

199.1

23.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.779 ± 0.161

0.66 ± 0.085

6.961 ± 0.166

9.256 ± 0.25

4.025 ± 0.106

5.723 ± 0.321

1.559 ± 0.087

7.257 ± 0.223

9.057 ± 0.245

7.932 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.122

6.971 ± 0.158

2.634 ± 0.139

3.94 ± 0.235

3.94 ± 0.123

5.999 ± 0.163

5.921 ± 0.163

5.886 ± 0.161

0.804 ± 0.061

5.283 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski