Spiroplasma endosymbiont of Megaselia nigra

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; unclassified Spiroplasma

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S0ST99|A0A3S0ST99_9MOLU Uncharacterized protein OS=Spiroplasma endosymbiont of Megaselia nigra OX=2478537 GN=D9R21_05755 PE=4 SV=1
MM1 pKa = 7.57NDD3 pKa = 3.23NVEE6 pKa = 4.25LLEE9 pKa = 4.81LVYY12 pKa = 10.82SYY14 pKa = 11.41LKK16 pKa = 10.43KK17 pKa = 10.57KK18 pKa = 10.4KK19 pKa = 10.49NSDD22 pKa = 3.06TFEE25 pKa = 4.9NIWNDD30 pKa = 3.0IASKK34 pKa = 10.94SDD36 pKa = 3.13VTVRR40 pKa = 11.84GASKK44 pKa = 10.46EE45 pKa = 3.95DD46 pKa = 3.8VIAEE50 pKa = 4.8LYY52 pKa = 9.48TDD54 pKa = 4.58LVLDD58 pKa = 3.6NRR60 pKa = 11.84FVLTSEE66 pKa = 4.45GMWGLCEE73 pKa = 3.73FLKK76 pKa = 10.83YY77 pKa = 10.75DD78 pKa = 4.49EE79 pKa = 5.02IKK81 pKa = 10.55KK82 pKa = 10.52QYY84 pKa = 10.82DD85 pKa = 3.31YY86 pKa = 11.33TDD88 pKa = 3.3RR89 pKa = 11.84FEE91 pKa = 4.28TTEE94 pKa = 3.96EE95 pKa = 4.23FEE97 pKa = 6.56DD98 pKa = 4.78IDD100 pKa = 5.46LADD103 pKa = 4.86DD104 pKa = 5.73DD105 pKa = 4.96EE106 pKa = 6.83DD107 pKa = 3.97EE108 pKa = 4.52EE109 pKa = 5.4KK110 pKa = 10.98NDD112 pKa = 3.91EE113 pKa = 4.26EE114 pKa = 4.37EE115 pKa = 4.46SLIDD119 pKa = 4.02TLDD122 pKa = 3.87FDD124 pKa = 4.8DD125 pKa = 4.94TLVDD129 pKa = 5.31DD130 pKa = 6.04DD131 pKa = 6.0LIEE134 pKa = 4.33NDD136 pKa = 3.79YY137 pKa = 11.21SINDD141 pKa = 3.83DD142 pKa = 4.38DD143 pKa = 4.15EE144 pKa = 4.65TVATKK149 pKa = 10.75LGITEE154 pKa = 4.44EE155 pKa = 4.33IDD157 pKa = 2.94WKK159 pKa = 11.04ALEE162 pKa = 5.14KK163 pKa = 10.88EE164 pKa = 4.19MEE166 pKa = 4.31EE167 pKa = 4.36NNN169 pKa = 3.57

Molecular weight: 19.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A432ZWL7|A0A432ZWL7_9MOLU Thiol peroxidase OS=Spiroplasma endosymbiont of Megaselia nigra OX=2478537 GN=D9R21_02320 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.14IKK11 pKa = 10.33HH12 pKa = 5.53KK13 pKa = 9.07RR14 pKa = 11.84THH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MEE23 pKa = 4.26SASGRR28 pKa = 11.84KK29 pKa = 9.01VLSKK33 pKa = 10.58RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.82GRR39 pKa = 11.84KK40 pKa = 8.62VLSAA44 pKa = 4.05

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1263

0

1263

352031

35

1454

278.7

32.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.994 ± 0.069

0.701 ± 0.017

5.184 ± 0.052

6.116 ± 0.074

4.826 ± 0.067

4.874 ± 0.062

1.475 ± 0.029

9.892 ± 0.068

9.48 ± 0.08

9.397 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.991 ± 0.029

8.165 ± 0.088

3.063 ± 0.041

3.995 ± 0.046

3.012 ± 0.047

5.593 ± 0.057

5.936 ± 0.047

5.482 ± 0.056

1.255 ± 0.031

4.569 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski