Flavobacterium phage FPSV-D15
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M2VBL3|A0A6M2VBL3_9CAUD SNF2_N domain-containing protein OS=Flavobacterium phage FPSV-D15 OX=2575338 PE=4 SV=1
MM1 pKa = 8.06 RR2 pKa = 11.84 NRR4 pKa = 11.84 IYY6 pKa = 10.25 IQYY9 pKa = 9.89 SGKK12 pKa = 8.17 ITKK15 pKa = 8.21 NTIALMEE22 pKa = 4.56 TYY24 pKa = 10.11 LICDD28 pKa = 4.78 FIGRR32 pKa = 11.84 TVIDD36 pKa = 3.57 GRR38 pKa = 11.84 RR39 pKa = 11.84 AYY41 pKa = 9.61 IDD43 pKa = 3.9 LKK45 pKa = 10.97 QSEE48 pKa = 4.73 NPNLDD53 pKa = 3.37 EE54 pKa = 4.11 VSQFILALDD63 pKa = 3.84 GTDD66 pKa = 6.66 FITFWGYY73 pKa = 9.86 PLEE76 pKa = 4.99 DD77 pKa = 3.88 GSNITTNEE85 pKa = 3.77 DD86 pKa = 3.29 FKK88 pKa = 11.48 DD89 pKa = 4.53 FSISEE94 pKa = 3.79 IDD96 pKa = 3.12 AA97 pKa = 4.2
Molecular weight: 11.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.345
IPC2_protein 4.279
IPC_protein 4.19
Toseland 3.999
ProMoST 4.342
Dawson 4.177
Bjellqvist 4.329
Wikipedia 4.101
Rodwell 4.024
Grimsley 3.91
Solomon 4.164
Lehninger 4.126
Nozaki 4.291
DTASelect 4.507
Thurlkill 4.037
EMBOSS 4.113
Sillero 4.317
Patrickios 3.694
IPC_peptide 4.164
IPC2_peptide 4.291
IPC2.peptide.svr19 4.236
Protein with the highest isoelectric point:
>tr|A0A6M2VAQ8|A0A6M2VAQ8_9CAUD Uncharacterized protein OS=Flavobacterium phage FPSV-D15 OX=2575338 PE=4 SV=1
MM1 pKa = 7.77 HH2 pKa = 6.82 IQILIMQQQPTIKK15 pKa = 10.4 LRR17 pKa = 11.84 DD18 pKa = 4.03 AIKK21 pKa = 10.7 KK22 pKa = 8.45 MRR24 pKa = 11.84 ALTGINVPFSFSYY37 pKa = 10.2 ISYY40 pKa = 10.62 SEE42 pKa = 4.16 VLNKK46 pKa = 10.35 SEE48 pKa = 4.35 GYY50 pKa = 10.05 KK51 pKa = 10.08 RR52 pKa = 11.84 VNKK55 pKa = 10.1 GLLRR59 pKa = 11.84 AGYY62 pKa = 9.35 RR63 pKa = 11.84 ANQSEE68 pKa = 4.24 RR69 pKa = 11.84 SNILIAYY76 pKa = 8.87 IDD78 pKa = 4.03 YY79 pKa = 10.62 DD80 pKa = 3.54 KK81 pKa = 11.66 KK82 pKa = 10.39 EE83 pKa = 4.11 ADD85 pKa = 3.69 RR86 pKa = 11.84 QFYY89 pKa = 10.85 LPLLMTFNNIQVIPP103 pKa = 3.92
Molecular weight: 12.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.145
IPC2_protein 9.458
IPC_protein 9.516
Toseland 9.853
ProMoST 9.648
Dawson 10.116
Bjellqvist 9.823
Wikipedia 10.321
Rodwell 10.423
Grimsley 10.218
Solomon 10.145
Lehninger 10.101
Nozaki 9.809
DTASelect 9.823
Thurlkill 9.94
EMBOSS 10.262
Sillero 10.028
Patrickios 7.571
IPC_peptide 10.145
IPC2_peptide 8.419
IPC2.peptide.svr19 8.509
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
63
0
63
14348
61
1464
227.7
26.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.638 ± 0.493
0.725 ± 0.127
5.597 ± 0.187
7.437 ± 0.235
4.969 ± 0.324
4.948 ± 0.26
1.61 ± 0.152
8.698 ± 0.29
9.653 ± 0.344
9.005 ± 0.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.335 ± 0.135
6.858 ± 0.25
2.795 ± 0.157
3.764 ± 0.142
3.757 ± 0.155
6.335 ± 0.214
5.164 ± 0.26
5.422 ± 0.184
1.164 ± 0.125
4.126 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here