Singulisphaera acidiphila (strain ATCC BAA-1392 / DSM 18658 / VKM B-2454 / MOB10)

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Isosphaerales; Isosphaeraceae; Singulisphaera; Singulisphaera acidiphila

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0DII1|L0DII1_SINAD Uncharacterized protein OS=Singulisphaera acidiphila (strain ATCC BAA-1392 / DSM 18658 / VKM B-2454 / MOB10) OX=886293 GN=Sinac_5045 PE=4 SV=1
MM1 pKa = 7.45FGNLRR6 pKa = 11.84ISVRR10 pKa = 11.84LIGLFVAICLATTAARR26 pKa = 11.84ADD28 pKa = 4.1DD29 pKa = 4.4PAPTIDD35 pKa = 4.89SPGNLDD41 pKa = 3.49NLSPLSEE48 pKa = 3.8ITFTITLPGKK58 pKa = 10.05IGTKK62 pKa = 9.87IIDD65 pKa = 4.0PSTIGIGVYY74 pKa = 10.23DD75 pKa = 4.1SNSDD79 pKa = 3.69EE80 pKa = 4.14IASFDD85 pKa = 3.7TTTVTSSVINHH96 pKa = 6.84PPNDD100 pKa = 3.8EE101 pKa = 4.29TPTSVTYY108 pKa = 8.91TFKK111 pKa = 10.51VTLPQSSSNYY121 pKa = 9.31SIEE124 pKa = 3.54IGAIYY129 pKa = 10.56DD130 pKa = 3.47GTQYY134 pKa = 11.87AWGDD138 pKa = 3.65YY139 pKa = 10.87NIATGDD145 pKa = 3.3

Molecular weight: 15.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0DQ60|L0DQ60_SINAD Cation/multidrug efflux pump OS=Singulisphaera acidiphila (strain ATCC BAA-1392 / DSM 18658 / VKM B-2454 / MOB10) OX=886293 GN=Sinac_7475 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84NGGQPGLGNGAGLPVHH18 pKa = 6.99RR19 pKa = 11.84GLNGGGNNLGGAANLNRR36 pKa = 11.84VQGLNQGMGSLGGIPTHH53 pKa = 7.0RR54 pKa = 11.84GGGMPQQNALGGMGGAAHH72 pKa = 5.96QFGGGGAMARR82 pKa = 11.84GSSAPAFNPGNMGGGARR99 pKa = 11.84GFSGGQMGGLGGARR113 pKa = 11.84GFSGGGHH120 pKa = 6.75AGGFSGGHH128 pKa = 6.1AGGFSGGGMGHH139 pKa = 6.91GGGGGHH145 pKa = 6.95GGGGHH150 pKa = 6.28RR151 pKa = 4.98

Molecular weight: 13.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7126

0

7126

2607485

29

9294

365.9

39.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.744 ± 0.031

0.943 ± 0.012

5.591 ± 0.023

5.783 ± 0.032

3.522 ± 0.017

8.412 ± 0.034

2.141 ± 0.017

4.554 ± 0.018

3.37 ± 0.031

10.476 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.917 ± 0.015

2.815 ± 0.027

6.099 ± 0.028

3.361 ± 0.019

7.505 ± 0.036

5.945 ± 0.029

5.627 ± 0.045

7.329 ± 0.025

1.555 ± 0.013

2.31 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski