Sistotremastrum suecicum HHB10207 ss-3
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13633 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A166AXZ1|A0A166AXZ1_9AGAM Uncharacterized protein OS=Sistotremastrum suecicum HHB10207 ss-3 OX=1314776 GN=SISSUDRAFT_127168 PE=4 SV=1
MM1 pKa = 7.18 QRR3 pKa = 11.84 IACSDD8 pKa = 3.33 NGFSLDD14 pKa = 3.37 EE15 pKa = 4.44 CQEE18 pKa = 4.02 QLSDD22 pKa = 3.82 CQGDD26 pKa = 4.1 GEE28 pKa = 5.12 SNLDD32 pKa = 3.38 LSFEE36 pKa = 4.52 VEE38 pKa = 4.91 FKK40 pKa = 11.16 ARR42 pKa = 11.84 DD43 pKa = 3.33 IEE45 pKa = 4.1 PRR47 pKa = 11.84 KK48 pKa = 8.45 MARR51 pKa = 11.84 RR52 pKa = 11.84 DD53 pKa = 3.55 DD54 pKa = 3.91 EE55 pKa = 4.17 GRR57 pKa = 11.84 EE58 pKa = 4.25 AGLSLGPGPFIPNAGLTPGFTTNSSTSLGANDD90 pKa = 4.06 TVPASTNTTLYY101 pKa = 10.83 MNMTLVTNATTDD113 pKa = 3.21 AYY115 pKa = 11.28 YY116 pKa = 9.69 STADD120 pKa = 3.8 PYY122 pKa = 10.59 ATPDD126 pKa = 3.31 SDD128 pKa = 4.01 PDD130 pKa = 3.7 VSVVGAYY137 pKa = 9.21 YY138 pKa = 9.21 QARR141 pKa = 11.84 YY142 pKa = 5.46 TTVDD146 pKa = 3.28 PYY148 pKa = 10.64 IAQGAYY154 pKa = 8.06 ATLTPTTPANIDD166 pKa = 3.46 PTSTSDD172 pKa = 3.27 PTIEE176 pKa = 3.94 TDD178 pKa = 3.76 QRR180 pKa = 11.84 LPSADD185 pKa = 3.74 PSEE188 pKa = 4.64 STPPPPPPPPPVDD201 pKa = 3.46 APGDD205 pKa = 3.68 QDD207 pKa = 3.58 GGEE210 pKa = 4.24 HH211 pKa = 6.16 GKK213 pKa = 8.24 EE214 pKa = 3.62 TSEE217 pKa = 3.85 YY218 pKa = 8.16 RR219 pKa = 11.84 THH221 pKa = 7.66 HH222 pKa = 7.15 DD223 pKa = 2.66 ISYY226 pKa = 9.45 LIPGTFGNEE235 pKa = 3.34 SDD237 pKa = 3.55
Molecular weight: 25.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.859
IPC_protein 3.872
Toseland 3.643
ProMoST 3.999
Dawson 3.859
Bjellqvist 4.037
Wikipedia 3.795
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.215
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.986
Patrickios 1.189
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.889
Protein with the highest isoelectric point:
>tr|A0A166HIK4|A0A166HIK4_9AGAM Acid protease OS=Sistotremastrum suecicum HHB10207 ss-3 OX=1314776 GN=SISSUDRAFT_844647 PE=3 SV=1
MM1 pKa = 7.39 PRR3 pKa = 11.84 IPFSLSRR10 pKa = 11.84 LLARR14 pKa = 11.84 PLPVVQRR21 pKa = 11.84 QPLVNRR27 pKa = 11.84 SLSLTTHH34 pKa = 7.13 LSSSIPRR41 pKa = 11.84 LSSSPILRR49 pKa = 11.84 AVSLLRR55 pKa = 11.84 SSISLLPTTPTASHH69 pKa = 7.22 LLQVRR74 pKa = 11.84 FNQRR78 pKa = 11.84 GVDD81 pKa = 3.75 YY82 pKa = 10.92 QPSQRR87 pKa = 11.84 KK88 pKa = 8.81 RR89 pKa = 11.84 KK90 pKa = 9.17 RR91 pKa = 11.84 KK92 pKa = 9.36 HH93 pKa = 5.58 GFLARR98 pKa = 11.84 LRR100 pKa = 11.84 SAGGRR105 pKa = 11.84 KK106 pKa = 8.73 ILARR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 AKK114 pKa = 9.28 GRR116 pKa = 11.84 KK117 pKa = 8.14 YY118 pKa = 10.54 LSHH121 pKa = 7.04
Molecular weight: 13.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.404
IPC2_protein 11.082
IPC_protein 12.442
Toseland 12.603
ProMoST 13.1
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.237
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.959
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13630
3
13633
5578206
50
4925
409.2
45.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.002 ± 0.017
1.266 ± 0.009
5.545 ± 0.014
5.892 ± 0.02
3.89 ± 0.013
6.163 ± 0.019
2.609 ± 0.01
5.251 ± 0.016
4.483 ± 0.016
9.597 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.969 ± 0.008
3.476 ± 0.01
6.572 ± 0.027
3.684 ± 0.013
6.161 ± 0.016
9.243 ± 0.026
6.017 ± 0.012
6.097 ± 0.014
1.474 ± 0.007
2.61 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here