Sistotremastrum suecicum HHB10207 ss-3

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Trechisporales; Trechisporales incertae sedis; Sistotremastrum; Sistotremastrum suecicum

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13633 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A166AXZ1|A0A166AXZ1_9AGAM Uncharacterized protein OS=Sistotremastrum suecicum HHB10207 ss-3 OX=1314776 GN=SISSUDRAFT_127168 PE=4 SV=1
MM1 pKa = 7.18QRR3 pKa = 11.84IACSDD8 pKa = 3.33NGFSLDD14 pKa = 3.37EE15 pKa = 4.44CQEE18 pKa = 4.02QLSDD22 pKa = 3.82CQGDD26 pKa = 4.1GEE28 pKa = 5.12SNLDD32 pKa = 3.38LSFEE36 pKa = 4.52VEE38 pKa = 4.91FKK40 pKa = 11.16ARR42 pKa = 11.84DD43 pKa = 3.33IEE45 pKa = 4.1PRR47 pKa = 11.84KK48 pKa = 8.45MARR51 pKa = 11.84RR52 pKa = 11.84DD53 pKa = 3.55DD54 pKa = 3.91EE55 pKa = 4.17GRR57 pKa = 11.84EE58 pKa = 4.25AGLSLGPGPFIPNAGLTPGFTTNSSTSLGANDD90 pKa = 4.06TVPASTNTTLYY101 pKa = 10.83MNMTLVTNATTDD113 pKa = 3.21AYY115 pKa = 11.28YY116 pKa = 9.69STADD120 pKa = 3.8PYY122 pKa = 10.59ATPDD126 pKa = 3.31SDD128 pKa = 4.01PDD130 pKa = 3.7VSVVGAYY137 pKa = 9.21YY138 pKa = 9.21QARR141 pKa = 11.84YY142 pKa = 5.46TTVDD146 pKa = 3.28PYY148 pKa = 10.64IAQGAYY154 pKa = 8.06ATLTPTTPANIDD166 pKa = 3.46PTSTSDD172 pKa = 3.27PTIEE176 pKa = 3.94TDD178 pKa = 3.76QRR180 pKa = 11.84LPSADD185 pKa = 3.74PSEE188 pKa = 4.64STPPPPPPPPPVDD201 pKa = 3.46APGDD205 pKa = 3.68QDD207 pKa = 3.58GGEE210 pKa = 4.24HH211 pKa = 6.16GKK213 pKa = 8.24EE214 pKa = 3.62TSEE217 pKa = 3.85YY218 pKa = 8.16RR219 pKa = 11.84THH221 pKa = 7.66HH222 pKa = 7.15DD223 pKa = 2.66ISYY226 pKa = 9.45LIPGTFGNEE235 pKa = 3.34SDD237 pKa = 3.55

Molecular weight:
25.3 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A166HIK4|A0A166HIK4_9AGAM Acid protease OS=Sistotremastrum suecicum HHB10207 ss-3 OX=1314776 GN=SISSUDRAFT_844647 PE=3 SV=1
MM1 pKa = 7.39PRR3 pKa = 11.84IPFSLSRR10 pKa = 11.84LLARR14 pKa = 11.84PLPVVQRR21 pKa = 11.84QPLVNRR27 pKa = 11.84SLSLTTHH34 pKa = 7.13LSSSIPRR41 pKa = 11.84LSSSPILRR49 pKa = 11.84AVSLLRR55 pKa = 11.84SSISLLPTTPTASHH69 pKa = 7.22LLQVRR74 pKa = 11.84FNQRR78 pKa = 11.84GVDD81 pKa = 3.75YY82 pKa = 10.92QPSQRR87 pKa = 11.84KK88 pKa = 8.81RR89 pKa = 11.84KK90 pKa = 9.17RR91 pKa = 11.84KK92 pKa = 9.36HH93 pKa = 5.58GFLARR98 pKa = 11.84LRR100 pKa = 11.84SAGGRR105 pKa = 11.84KK106 pKa = 8.73ILARR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84AKK114 pKa = 9.28GRR116 pKa = 11.84KK117 pKa = 8.14YY118 pKa = 10.54LSHH121 pKa = 7.04

Molecular weight:
13.72 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13630

3

13633

5578206

50

4925

409.2

45.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.002 ± 0.017

1.266 ± 0.009

5.545 ± 0.014

5.892 ± 0.02

3.89 ± 0.013

6.163 ± 0.019

2.609 ± 0.01

5.251 ± 0.016

4.483 ± 0.016

9.597 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.969 ± 0.008

3.476 ± 0.01

6.572 ± 0.027

3.684 ± 0.013

6.161 ± 0.016

9.243 ± 0.026

6.017 ± 0.012

6.097 ± 0.014

1.474 ± 0.007

2.61 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski