Thioflexothrix psekupsii
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3187 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A251XA25|A0A251XA25_9GAMM Uncharacterized protein OS=Thioflexothrix psekupsii OX=1570016 GN=TPSD3_09580 PE=4 SV=1
MM1 pKa = 7.52 SLQNSLDD8 pKa = 3.38 GLTEE12 pKa = 3.63 EE13 pKa = 4.03 LRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 EE20 pKa = 3.79 AQEE23 pKa = 5.27 SIDD26 pKa = 4.06 SCCDD30 pKa = 3.13 TSDD33 pKa = 4.56 DD34 pKa = 3.52 LHH36 pKa = 7.72 GFYY39 pKa = 10.82 GVDD42 pKa = 3.2 SDD44 pKa = 6.35 DD45 pKa = 3.39 IVSYY49 pKa = 11.04 DD50 pKa = 3.98 FNHH53 pKa = 7.06 HH54 pKa = 7.11 ANDD57 pKa = 3.74 QYY59 pKa = 11.5 EE60 pKa = 4.52 PPHH63 pKa = 8.16 SDD65 pKa = 3.71 DD66 pKa = 3.79 FCDD69 pKa = 5.28 DD70 pKa = 3.48 VSSDD74 pKa = 3.21
Molecular weight: 8.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.923
IPC_protein 3.91
Toseland 3.681
ProMoST 4.024
Dawson 3.923
Bjellqvist 4.113
Wikipedia 3.884
Rodwell 3.732
Grimsley 3.592
Solomon 3.91
Lehninger 3.859
Nozaki 4.037
DTASelect 4.317
Thurlkill 3.757
EMBOSS 3.884
Sillero 4.037
Patrickios 1.939
IPC_peptide 3.897
IPC2_peptide 4.012
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|A0A251X694|A0A251X694_9GAMM Uncharacterized protein OS=Thioflexothrix psekupsii OX=1570016 GN=TPSD3_10880 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.5 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 9.02 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIQARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.51 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3187
0
3187
1126285
21
4445
353.4
39.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.509 ± 0.052
1.018 ± 0.015
5.16 ± 0.04
6.296 ± 0.048
4.116 ± 0.031
6.166 ± 0.053
2.68 ± 0.027
6.294 ± 0.037
4.414 ± 0.042
11.541 ± 0.061
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.249 ± 0.019
4.045 ± 0.032
4.371 ± 0.033
5.507 ± 0.043
5.355 ± 0.032
5.792 ± 0.036
5.481 ± 0.047
6.445 ± 0.046
1.366 ± 0.02
3.196 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here