Proteome-pI 2.0 - Proteome Isoelectric Point Database

Tomato leaf curl Java virus-[Ageratum] satellite DNA

Taxonomy: Viruses; Tolecusatellitidae; Betasatellite; Tomato leaf curl Java betasatellite

Average proteome isoelectric point is 5.04

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|Q75QI1|Q75QI1_9VIRU Beta C1 OS=Tomato leaf curl Java virus-[Ageratum] satellite DNA OX=263578 GN=beta C1 PE=4 SV=1

MM1 pKa = 7.32TISYY5 pKa = 10.53SNNRR9 pKa = 11.84GMMFIIQVRR18 pKa = 11.84LSSEE22 pKa = 3.57IQITVEE28 pKa = 3.66LEE30 pKa = 4.07MYY32 pKa = 7.99STRR35 pKa = 11.84SPALVRR41 pKa = 11.84HH42 pKa = 6.09KK43 pKa = 10.61FSIPYY48 pKa = 8.11GHH50 pKa = 6.72NGIIPPFNFNALEE63 pKa = 4.01EE64 pKa = 4.94GIKK67 pKa = 10.38NLLRR71 pKa = 11.84IMYY74 pKa = 9.29KK75 pKa = 10.41DD76 pKa = 3.34STIEE80 pKa = 3.98EE81 pKa = 4.15IKK83 pKa = 11.0AEE85 pKa = 4.08DD86 pKa = 3.62MVEE89 pKa = 4.24IIDD92 pKa = 4.6MMMQQEE98 pKa = 4.52APVIDD103 pKa = 3.35IQVNDD108 pKa = 3.78EE109 pKa = 4.0YY110 pKa = 10.99RR111 pKa = 11.84VYY113 pKa = 11.24KK114 pKa = 10.49HH115 pKa = 5.05VTVV118 pKa = 3.93

Molecular weight: 13.77 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|Q75QI1|Q75QI1_9VIRU Beta C1 OS=Tomato leaf curl Java virus-[Ageratum] satellite DNA OX=263578 GN=beta C1 PE=4 SV=1

MM1 pKa = 7.32TISYY5 pKa = 10.53SNNRR9 pKa = 11.84GMMFIIQVRR18 pKa = 11.84LSSEE22 pKa = 3.57IQITVEE28 pKa = 3.66LEE30 pKa = 4.07MYY32 pKa = 7.99STRR35 pKa = 11.84SPALVRR41 pKa = 11.84HH42 pKa = 6.09KK43 pKa = 10.61FSIPYY48 pKa = 8.11GHH50 pKa = 6.72NGIIPPFNFNALEE63 pKa = 4.01EE64 pKa = 4.94GIKK67 pKa = 10.38NLLRR71 pKa = 11.84IMYY74 pKa = 9.29KK75 pKa = 10.41DD76 pKa = 3.34STIEE80 pKa = 3.98EE81 pKa = 4.15IKK83 pKa = 11.0AEE85 pKa = 4.08DD86 pKa = 3.62MVEE89 pKa = 4.24IIDD92 pKa = 4.6MMMQQEE98 pKa = 4.52APVIDD103 pKa = 3.35IQVNDD108 pKa = 3.78EE109 pKa = 4.0YY110 pKa = 10.99RR111 pKa = 11.84VYY113 pKa = 11.24KK114 pKa = 10.49HH115 pKa = 5.05VTVV118 pKa = 3.93

Molecular weight: 13.77 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	118	118	118	118.0	13.77

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
3.39 ± 0.0	0.0 ± 0.0	4.237 ± 0.0	9.322 ± 0.0	3.39 ± 0.0	3.39 ± 0.0	2.542 ± 0.0	13.559 ± 0.0	4.237 ± 0.0	5.085 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
7.627 ± 0.0	5.932 ± 0.0	4.237 ± 0.0	4.237 ± 0.0	5.085 ± 0.0	6.78 ± 0.0	4.237 ± 0.0	7.627 ± 0.0	0.0 ± 0.0	5.085 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski