Chalara elegans RNA Virus 1
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6Q425|Q6Q425_9VIRU Coat protein OS=Chalara elegans RNA Virus 1 OX=267285 PE=4 SV=1
MM1 pKa = 7.58 EE2 pKa = 4.73 ATSLNHH8 pKa = 6.28 FLSGVIAAPKK18 pKa = 9.75 GGRR21 pKa = 11.84 LAEE24 pKa = 4.35 DD25 pKa = 3.44 SAFRR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 9.13 RR32 pKa = 11.84 AHH34 pKa = 6.3 VRR36 pKa = 11.84 TTATIGGNTDD46 pKa = 2.62 ARR48 pKa = 11.84 NAFIRR53 pKa = 11.84 YY54 pKa = 7.66 EE55 pKa = 4.13 VGRR58 pKa = 11.84 SSGKK62 pKa = 10.18 RR63 pKa = 11.84 GQLLAAPPDD72 pKa = 3.54 DD73 pKa = 3.58 SRR75 pKa = 11.84 RR76 pKa = 11.84 IEE78 pKa = 3.95 ASYY81 pKa = 8.29 PTNAVLAEE89 pKa = 4.51 DD90 pKa = 4.25 FLGLAKK96 pKa = 10.32 KK97 pKa = 10.38 YY98 pKa = 10.88 SNFSAQFQFSSLAAVAEE115 pKa = 4.22 RR116 pKa = 11.84 LAKK119 pKa = 10.34 GLAVHH124 pKa = 6.44 ATVADD129 pKa = 3.73 VDD131 pKa = 4.57 SVALRR136 pKa = 11.84 GGAPLIVAGLGTYY149 pKa = 9.96 DD150 pKa = 3.84 GPINSLISSVFIPRR164 pKa = 11.84 LVNNVLTGDD173 pKa = 3.96 VFSVLANAIAGEE185 pKa = 4.34 GASIATDD192 pKa = 3.04 IAEE195 pKa = 4.66 IDD197 pKa = 3.59 PSTRR201 pKa = 11.84 QPVIPEE207 pKa = 3.61 VDD209 pKa = 2.93 GDD211 pKa = 4.3 GFAKK215 pKa = 10.57 AATEE219 pKa = 3.73 ALRR222 pKa = 11.84 IVGANMAQSDD232 pKa = 3.73 QGLLFSLAVTRR243 pKa = 11.84 GIHH246 pKa = 6.41 SVVSWLPTAMKK257 pKa = 10.28 AALLGICCVWTFQCPFVGFITGSKK281 pKa = 9.69 LHH283 pKa = 6.6 GPPSLLSTVSRR294 pKa = 11.84 SVLLCRR300 pKa = 11.84 CHH302 pKa = 6.17 CTDD305 pKa = 3.73 PAAAVAPSDD314 pKa = 3.76 PGEE317 pKa = 4.33 NYY319 pKa = 10.15 NGEE322 pKa = 4.24 WFPTFYY328 pKa = 10.8 SGTKK332 pKa = 10.37 GGDD335 pKa = 3.15 PTVRR339 pKa = 11.84 PGGDD343 pKa = 3.21 CPGNSEE349 pKa = 3.84 TSGRR353 pKa = 11.84 IRR355 pKa = 11.84 SQLLADD361 pKa = 3.76 CEE363 pKa = 4.46 KK364 pKa = 10.61 FFRR367 pKa = 11.84 NYY369 pKa = 9.52 IPALGRR375 pKa = 11.84 IFGLTGSPNLAVTVAVGMSRR395 pKa = 11.84 FLHH398 pKa = 6.47 ADD400 pKa = 3.35 PRR402 pKa = 11.84 HH403 pKa = 5.52 LRR405 pKa = 11.84 YY406 pKa = 10.1 ASVAPWYY413 pKa = 9.16 WIEE416 pKa = 4.11 PTSLLPSDD424 pKa = 4.32 FLGSVAEE431 pKa = 4.3 MNGSGSFGGKK441 pKa = 10.05 DD442 pKa = 3.21 STKK445 pKa = 10.67 TKK447 pKa = 10.42 LAWEE451 pKa = 5.06 DD452 pKa = 3.22 IEE454 pKa = 5.09 LAGDD458 pKa = 3.54 RR459 pKa = 11.84 DD460 pKa = 4.4 TTFSAYY466 pKa = 9.51 RR467 pKa = 11.84 AKK469 pKa = 10.46 FLSPRR474 pKa = 11.84 RR475 pKa = 11.84 AWFMAHH481 pKa = 6.06 WNGHH485 pKa = 5.58 PDD487 pKa = 3.35 NGLGCIRR494 pKa = 11.84 LRR496 pKa = 11.84 QADD499 pKa = 4.1 PNGFIHH505 pKa = 7.26 PGRR508 pKa = 11.84 GTSGADD514 pKa = 3.07 LRR516 pKa = 11.84 DD517 pKa = 3.75 RR518 pKa = 11.84 LEE520 pKa = 4.98 DD521 pKa = 4.0 DD522 pKa = 5.15 APISDD527 pKa = 4.25 YY528 pKa = 11.34 LWDD531 pKa = 4.52 RR532 pKa = 11.84 GQSPFCAPGEE542 pKa = 3.85 LLNLGSTIGFLVRR555 pKa = 11.84 HH556 pKa = 5.21 VTFDD560 pKa = 3.76 DD561 pKa = 4.27 DD562 pKa = 5.27 GIPTTEE568 pKa = 4.18 HH569 pKa = 6.1 VPTSRR574 pKa = 11.84 EE575 pKa = 3.93 FLDD578 pKa = 3.47 TTVTIEE584 pKa = 3.67 VGRR587 pKa = 11.84 PLSISIGKK595 pKa = 8.79 SNCPDD600 pKa = 3.0 SKK602 pKa = 10.99 ARR604 pKa = 11.84 RR605 pKa = 11.84 ARR607 pKa = 11.84 TRR609 pKa = 11.84 ATIEE613 pKa = 3.63 LGAASRR619 pKa = 11.84 RR620 pKa = 11.84 ARR622 pKa = 11.84 AFGSAAVAEE631 pKa = 4.61 MPTLSTAPRR640 pKa = 11.84 ALTFAPSRR648 pKa = 11.84 LMVKK652 pKa = 9.34 DD653 pKa = 3.36 TGGGAGSSRR662 pKa = 11.84 HH663 pKa = 5.65 GADD666 pKa = 3.19 SGGSGADD673 pKa = 3.34 DD674 pKa = 3.94 AARR677 pKa = 11.84 KK678 pKa = 9.7 ADD680 pKa = 3.83 GVPVRR685 pKa = 11.84 ATAHH689 pKa = 5.02 NQPVRR694 pKa = 11.84 FPTLANPTGPTRR706 pKa = 11.84 SSAAPAAAARR716 pKa = 11.84 DD717 pKa = 3.83 QSAGGPSVDD726 pKa = 3.17 IVRR729 pKa = 11.84 SGGDD733 pKa = 2.99 AASEE737 pKa = 4.35 VGSDD741 pKa = 3.76 VAPAPAPGVEE751 pKa = 4.2 SGVVSSSVAAPTNEE765 pKa = 3.24 GRR767 pKa = 11.84 NDD769 pKa = 3.16 VV770 pKa = 3.77
Molecular weight: 80.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.48
IPC2_protein 6.44
IPC_protein 6.532
Toseland 6.634
ProMoST 6.985
Dawson 6.971
Bjellqvist 6.956
Wikipedia 6.956
Rodwell 6.971
Grimsley 6.751
Solomon 6.985
Lehninger 6.985
Nozaki 7.278
DTASelect 7.351
Thurlkill 7.38
EMBOSS 7.366
Sillero 7.395
Patrickios 4.546
IPC_peptide 6.985
IPC2_peptide 7.102
IPC2.peptide.svr19 6.902
Protein with the highest isoelectric point:
>tr|Q6Q425|Q6Q425_9VIRU Coat protein OS=Chalara elegans RNA Virus 1 OX=267285 PE=4 SV=1
MM1 pKa = 7.58 EE2 pKa = 4.73 ATSLNHH8 pKa = 6.28 FLSGVIAAPKK18 pKa = 9.75 GGRR21 pKa = 11.84 LAEE24 pKa = 4.35 DD25 pKa = 3.44 SAFRR29 pKa = 11.84 RR30 pKa = 11.84 YY31 pKa = 9.13 RR32 pKa = 11.84 AHH34 pKa = 6.3 VRR36 pKa = 11.84 TTATIGGNTDD46 pKa = 2.62 ARR48 pKa = 11.84 NAFIRR53 pKa = 11.84 YY54 pKa = 7.66 EE55 pKa = 4.13 VGRR58 pKa = 11.84 SSGKK62 pKa = 10.18 RR63 pKa = 11.84 GQLLAAPPDD72 pKa = 3.54 DD73 pKa = 3.58 SRR75 pKa = 11.84 RR76 pKa = 11.84 IEE78 pKa = 3.95 ASYY81 pKa = 8.29 PTNAVLAEE89 pKa = 4.51 DD90 pKa = 4.25 FLGLAKK96 pKa = 10.32 KK97 pKa = 10.38 YY98 pKa = 10.88 SNFSAQFQFSSLAAVAEE115 pKa = 4.22 RR116 pKa = 11.84 LAKK119 pKa = 10.34 GLAVHH124 pKa = 6.44 ATVADD129 pKa = 3.73 VDD131 pKa = 4.57 SVALRR136 pKa = 11.84 GGAPLIVAGLGTYY149 pKa = 9.96 DD150 pKa = 3.84 GPINSLISSVFIPRR164 pKa = 11.84 LVNNVLTGDD173 pKa = 3.96 VFSVLANAIAGEE185 pKa = 4.34 GASIATDD192 pKa = 3.04 IAEE195 pKa = 4.66 IDD197 pKa = 3.59 PSTRR201 pKa = 11.84 QPVIPEE207 pKa = 3.61 VDD209 pKa = 2.93 GDD211 pKa = 4.3 GFAKK215 pKa = 10.57 AATEE219 pKa = 3.73 ALRR222 pKa = 11.84 IVGANMAQSDD232 pKa = 3.73 QGLLFSLAVTRR243 pKa = 11.84 GIHH246 pKa = 6.41 SVVSWLPTAMKK257 pKa = 10.28 AALLGICCVWTFQCPFVGFITGSKK281 pKa = 9.69 LHH283 pKa = 6.6 GPPSLLSTVSRR294 pKa = 11.84 SVLLCRR300 pKa = 11.84 CHH302 pKa = 6.17 CTDD305 pKa = 3.73 PAAAVAPSDD314 pKa = 3.76 PGEE317 pKa = 4.33 NYY319 pKa = 10.15 NGEE322 pKa = 4.24 WFPTFYY328 pKa = 10.8 SGTKK332 pKa = 10.37 GGDD335 pKa = 3.15 PTVRR339 pKa = 11.84 PGGDD343 pKa = 3.21 CPGNSEE349 pKa = 3.84 TSGRR353 pKa = 11.84 IRR355 pKa = 11.84 SQLLADD361 pKa = 3.76 CEE363 pKa = 4.46 KK364 pKa = 10.61 FFRR367 pKa = 11.84 NYY369 pKa = 9.52 IPALGRR375 pKa = 11.84 IFGLTGSPNLAVTVAVGMSRR395 pKa = 11.84 FLHH398 pKa = 6.47 ADD400 pKa = 3.35 PRR402 pKa = 11.84 HH403 pKa = 5.52 LRR405 pKa = 11.84 YY406 pKa = 10.1 ASVAPWYY413 pKa = 9.16 WIEE416 pKa = 4.11 PTSLLPSDD424 pKa = 4.32 FLGSVAEE431 pKa = 4.3 MNGSGSFGGKK441 pKa = 10.05 DD442 pKa = 3.21 STKK445 pKa = 10.67 TKK447 pKa = 10.42 LAWEE451 pKa = 5.06 DD452 pKa = 3.22 IEE454 pKa = 5.09 LAGDD458 pKa = 3.54 RR459 pKa = 11.84 DD460 pKa = 4.4 TTFSAYY466 pKa = 9.51 RR467 pKa = 11.84 AKK469 pKa = 10.46 FLSPRR474 pKa = 11.84 RR475 pKa = 11.84 AWFMAHH481 pKa = 6.06 WNGHH485 pKa = 5.58 PDD487 pKa = 3.35 NGLGCIRR494 pKa = 11.84 LRR496 pKa = 11.84 QADD499 pKa = 4.1 PNGFIHH505 pKa = 7.26 PGRR508 pKa = 11.84 GTSGADD514 pKa = 3.07 LRR516 pKa = 11.84 DD517 pKa = 3.75 RR518 pKa = 11.84 LEE520 pKa = 4.98 DD521 pKa = 4.0 DD522 pKa = 5.15 APISDD527 pKa = 4.25 YY528 pKa = 11.34 LWDD531 pKa = 4.52 RR532 pKa = 11.84 GQSPFCAPGEE542 pKa = 3.85 LLNLGSTIGFLVRR555 pKa = 11.84 HH556 pKa = 5.21 VTFDD560 pKa = 3.76 DD561 pKa = 4.27 DD562 pKa = 5.27 GIPTTEE568 pKa = 4.18 HH569 pKa = 6.1 VPTSRR574 pKa = 11.84 EE575 pKa = 3.93 FLDD578 pKa = 3.47 TTVTIEE584 pKa = 3.67 VGRR587 pKa = 11.84 PLSISIGKK595 pKa = 8.79 SNCPDD600 pKa = 3.0 SKK602 pKa = 10.99 ARR604 pKa = 11.84 RR605 pKa = 11.84 ARR607 pKa = 11.84 TRR609 pKa = 11.84 ATIEE613 pKa = 3.63 LGAASRR619 pKa = 11.84 RR620 pKa = 11.84 ARR622 pKa = 11.84 AFGSAAVAEE631 pKa = 4.61 MPTLSTAPRR640 pKa = 11.84 ALTFAPSRR648 pKa = 11.84 LMVKK652 pKa = 9.34 DD653 pKa = 3.36 TGGGAGSSRR662 pKa = 11.84 HH663 pKa = 5.65 GADD666 pKa = 3.19 SGGSGADD673 pKa = 3.34 DD674 pKa = 3.94 AARR677 pKa = 11.84 KK678 pKa = 9.7 ADD680 pKa = 3.83 GVPVRR685 pKa = 11.84 ATAHH689 pKa = 5.02 NQPVRR694 pKa = 11.84 FPTLANPTGPTRR706 pKa = 11.84 SSAAPAAAARR716 pKa = 11.84 DD717 pKa = 3.83 QSAGGPSVDD726 pKa = 3.17 IVRR729 pKa = 11.84 SGGDD733 pKa = 2.99 AASEE737 pKa = 4.35 VGSDD741 pKa = 3.76 VAPAPAPGVEE751 pKa = 4.2 SGVVSSSVAAPTNEE765 pKa = 3.24 GRR767 pKa = 11.84 NDD769 pKa = 3.16 VV770 pKa = 3.77
Molecular weight: 80.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.48
IPC2_protein 6.44
IPC_protein 6.532
Toseland 6.634
ProMoST 6.985
Dawson 6.971
Bjellqvist 6.956
Wikipedia 6.956
Rodwell 6.971
Grimsley 6.751
Solomon 6.985
Lehninger 6.985
Nozaki 7.278
DTASelect 7.351
Thurlkill 7.38
EMBOSS 7.366
Sillero 7.395
Patrickios 4.546
IPC_peptide 6.985
IPC2_peptide 7.102
IPC2.peptide.svr19 6.902
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
770
770
770
770.0
80.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.987 ± 0.0
1.429 ± 0.0
6.234 ± 0.0
3.636 ± 0.0
4.286 ± 0.0
10.39 ± 0.0
1.948 ± 0.0
4.156 ± 0.0
2.338 ± 0.0
7.662 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.039 ± 0.0
3.247 ± 0.0
6.753 ± 0.0
1.558 ± 0.0
7.403 ± 0.0
9.221 ± 0.0
6.364 ± 0.0
6.623 ± 0.0
1.169 ± 0.0
1.558 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here