Amino acid dipepetide frequency for Staphylococcus phage P282

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.723AlaAla: 1.723 ± 0.771
0.164AlaCys: 0.164 ± 0.107
2.134AlaAsp: 2.134 ± 0.427
3.775AlaGlu: 3.775 ± 0.665
3.036AlaPhe: 3.036 ± 0.614
3.365AlaGly: 3.365 ± 0.616
0.821AlaHis: 0.821 ± 0.286
5.744AlaIle: 5.744 ± 0.656
4.76AlaLys: 4.76 ± 0.592
4.677AlaLeu: 4.677 ± 0.713
1.723AlaMet: 1.723 ± 0.448
2.79AlaAsn: 2.79 ± 0.527
1.723AlaPro: 1.723 ± 0.327
3.2AlaGln: 3.2 ± 0.534
2.79AlaArg: 2.79 ± 0.483
3.365AlaSer: 3.365 ± 0.694
4.021AlaThr: 4.021 ± 0.656
3.036AlaVal: 3.036 ± 0.645
0.41AlaTrp: 0.41 ± 0.203
2.954AlaTyr: 2.954 ± 0.431
0.0AlaXaa: 0.0 ± 0.0
Cys
0.41CysAla: 0.41 ± 0.204
0.0CysCys: 0.0 ± 0.0
0.082CysAsp: 0.082 ± 0.073
0.41CysGlu: 0.41 ± 0.239
0.246CysPhe: 0.246 ± 0.148
0.246CysGly: 0.246 ± 0.184
0.164CysHis: 0.164 ± 0.112
0.903CysIle: 0.903 ± 0.246
0.492CysLys: 0.492 ± 0.285
0.246CysLeu: 0.246 ± 0.131
0.164CysMet: 0.164 ± 0.198
0.164CysAsn: 0.164 ± 0.099
0.164CysPro: 0.164 ± 0.124
0.082CysGln: 0.082 ± 0.086
0.41CysArg: 0.41 ± 0.259
0.328CysSer: 0.328 ± 0.202
0.328CysThr: 0.328 ± 0.159
0.328CysVal: 0.328 ± 0.185
0.082CysTrp: 0.082 ± 0.073
0.164CysTyr: 0.164 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
3.611AspAla: 3.611 ± 0.575
0.492AspCys: 0.492 ± 0.195
3.775AspAsp: 3.775 ± 0.692
5.662AspGlu: 5.662 ± 0.956
3.036AspPhe: 3.036 ± 0.461
5.17AspGly: 5.17 ± 0.795
0.41AspHis: 0.41 ± 0.187
4.842AspIle: 4.842 ± 0.593
5.662AspLys: 5.662 ± 0.765
5.006AspLeu: 5.006 ± 0.698
2.134AspMet: 2.134 ± 0.415
4.103AspAsn: 4.103 ± 0.722
1.395AspPro: 1.395 ± 0.398
0.821AspGln: 0.821 ± 0.188
2.216AspArg: 2.216 ± 0.511
3.2AspSer: 3.2 ± 0.62
2.544AspThr: 2.544 ± 0.634
4.021AspVal: 4.021 ± 0.561
0.574AspTrp: 0.574 ± 0.234
2.954AspTyr: 2.954 ± 0.608
0.0AspXaa: 0.0 ± 0.0
Glu
4.513GluAla: 4.513 ± 0.726
0.739GluCys: 0.739 ± 0.308
4.595GluAsp: 4.595 ± 0.831
7.632GluGlu: 7.632 ± 1.076
3.365GluPhe: 3.365 ± 0.412
3.118GluGly: 3.118 ± 0.527
1.723GluHis: 1.723 ± 0.414
6.401GluIle: 6.401 ± 0.805
7.221GluLys: 7.221 ± 0.952
7.303GluLeu: 7.303 ± 0.889
1.887GluMet: 1.887 ± 0.424
5.58GluAsn: 5.58 ± 0.584
1.723GluPro: 1.723 ± 0.36
4.103GluGln: 4.103 ± 0.725
4.349GluArg: 4.349 ± 0.71
4.185GluSer: 4.185 ± 0.672
3.693GluThr: 3.693 ± 0.8
4.595GluVal: 4.595 ± 0.532
1.231GluTrp: 1.231 ± 0.344
5.006GluTyr: 5.006 ± 0.729
0.0GluXaa: 0.0 ± 0.0
Phe
2.79PheAla: 2.79 ± 0.6
0.246PheCys: 0.246 ± 0.148
2.954PheAsp: 2.954 ± 0.445
3.2PheGlu: 3.2 ± 0.437
1.149PhePhe: 1.149 ± 0.355
2.134PheGly: 2.134 ± 0.39
0.656PheHis: 0.656 ± 0.244
3.447PheIle: 3.447 ± 0.565
4.185PheLys: 4.185 ± 0.698
3.036PheLeu: 3.036 ± 0.626
1.477PheMet: 1.477 ± 0.46
3.529PheAsn: 3.529 ± 0.57
0.903PhePro: 0.903 ± 0.27
0.492PheGln: 0.492 ± 0.194
1.723PheArg: 1.723 ± 0.34
3.611PheSer: 3.611 ± 0.823
3.118PheThr: 3.118 ± 0.589
2.298PheVal: 2.298 ± 0.477
0.164PheTrp: 0.164 ± 0.12
1.395PheTyr: 1.395 ± 0.332
0.0PheXaa: 0.0 ± 0.0
Gly
2.626GlyAla: 2.626 ± 0.794
0.492GlyCys: 0.492 ± 0.238
3.693GlyAsp: 3.693 ± 0.639
3.447GlyGlu: 3.447 ± 0.791
2.708GlyPhe: 2.708 ± 0.561
3.857GlyGly: 3.857 ± 1.12
1.149GlyHis: 1.149 ± 0.213
4.103GlyIle: 4.103 ± 0.793
6.893GlyLys: 6.893 ± 0.722
5.58GlyLeu: 5.58 ± 0.922
1.149GlyMet: 1.149 ± 0.332
2.79GlyAsn: 2.79 ± 0.465
1.313GlyPro: 1.313 ± 0.334
1.559GlyGln: 1.559 ± 0.42
2.052GlyArg: 2.052 ± 0.566
2.708GlySer: 2.708 ± 0.459
2.79GlyThr: 2.79 ± 0.461
3.775GlyVal: 3.775 ± 0.649
0.903GlyTrp: 0.903 ± 0.303
3.118GlyTyr: 3.118 ± 0.589
0.0GlyXaa: 0.0 ± 0.0
His
1.231HisAla: 1.231 ± 0.426
0.082HisCys: 0.082 ± 0.087
0.821HisAsp: 0.821 ± 0.259
1.149HisGlu: 1.149 ± 0.34
1.395HisPhe: 1.395 ± 0.31
0.492HisGly: 0.492 ± 0.281
0.41HisHis: 0.41 ± 0.267
2.134HisIle: 2.134 ± 0.409
1.395HisLys: 1.395 ± 0.38
1.231HisLeu: 1.231 ± 0.266
0.164HisMet: 0.164 ± 0.107
0.985HisAsn: 0.985 ± 0.286
0.41HisPro: 0.41 ± 0.165
0.492HisGln: 0.492 ± 0.171
0.246HisArg: 0.246 ± 0.153
0.656HisSer: 0.656 ± 0.163
0.739HisThr: 0.739 ± 0.321
0.985HisVal: 0.985 ± 0.266
0.082HisTrp: 0.082 ± 0.092
1.313HisTyr: 1.313 ± 0.342
0.0HisXaa: 0.0 ± 0.0
Ile
5.334IleAla: 5.334 ± 0.873
0.574IleCys: 0.574 ± 0.242
5.99IleAsp: 5.99 ± 0.724
6.893IleGlu: 6.893 ± 0.709
3.611IlePhe: 3.611 ± 0.62
3.939IleGly: 3.939 ± 0.607
1.231IleHis: 1.231 ± 0.414
4.595IleIle: 4.595 ± 0.904
8.863IleLys: 8.863 ± 0.738
3.693IleLeu: 3.693 ± 0.488
1.641IleMet: 1.641 ± 0.35
6.483IleAsn: 6.483 ± 1.137
2.462IlePro: 2.462 ± 0.517
2.954IleGln: 2.954 ± 0.435
3.447IleArg: 3.447 ± 0.554
5.088IleSer: 5.088 ± 0.757
4.924IleThr: 4.924 ± 0.603
4.842IleVal: 4.842 ± 0.604
1.149IleTrp: 1.149 ± 0.444
3.036IleTyr: 3.036 ± 0.689
0.0IleXaa: 0.0 ± 0.0
Lys
5.744LysAla: 5.744 ± 0.464
0.328LysCys: 0.328 ± 0.25
5.908LysAsp: 5.908 ± 0.665
9.027LysGlu: 9.027 ± 0.897
4.021LysPhe: 4.021 ± 0.617
6.073LysGly: 6.073 ± 0.956
1.559LysHis: 1.559 ± 0.519
7.468LysIle: 7.468 ± 0.753
7.878LysLys: 7.878 ± 0.88
8.124LysLeu: 8.124 ± 0.839
2.872LysMet: 2.872 ± 0.6
6.155LysAsn: 6.155 ± 0.887
2.626LysPro: 2.626 ± 0.544
4.103LysGln: 4.103 ± 0.492
4.185LysArg: 4.185 ± 0.578
5.006LysSer: 5.006 ± 0.699
5.334LysThr: 5.334 ± 0.759
5.744LysVal: 5.744 ± 0.685
1.067LysTrp: 1.067 ± 0.288
4.513LysTyr: 4.513 ± 0.688
0.0LysXaa: 0.0 ± 0.0
Leu
4.349LeuAla: 4.349 ± 0.695
0.41LeuCys: 0.41 ± 0.18
4.513LeuAsp: 4.513 ± 0.572
6.975LeuGlu: 6.975 ± 0.908
2.872LeuPhe: 2.872 ± 0.466
3.282LeuGly: 3.282 ± 0.675
0.985LeuHis: 0.985 ± 0.296
5.252LeuIle: 5.252 ± 0.73
8.699LeuLys: 8.699 ± 0.885
6.483LeuLeu: 6.483 ± 0.898
1.969LeuMet: 1.969 ± 0.514
5.252LeuAsn: 5.252 ± 0.626
2.544LeuPro: 2.544 ± 0.452
2.708LeuGln: 2.708 ± 0.45
2.954LeuArg: 2.954 ± 0.575
5.744LeuSer: 5.744 ± 0.663
4.349LeuThr: 4.349 ± 0.664
3.857LeuVal: 3.857 ± 0.613
0.739LeuTrp: 0.739 ± 0.221
3.118LeuTyr: 3.118 ± 0.516
0.0LeuXaa: 0.0 ± 0.0
Met
0.985MetAla: 0.985 ± 0.35
0.164MetCys: 0.164 ± 0.123
1.231MetAsp: 1.231 ± 0.321
0.985MetGlu: 0.985 ± 0.314
0.656MetPhe: 0.656 ± 0.193
1.477MetGly: 1.477 ± 0.512
0.246MetHis: 0.246 ± 0.196
2.298MetIle: 2.298 ± 0.427
2.626MetLys: 2.626 ± 0.527
2.052MetLeu: 2.052 ± 0.402
0.821MetMet: 0.821 ± 0.278
1.887MetAsn: 1.887 ± 0.429
0.821MetPro: 0.821 ± 0.228
0.903MetGln: 0.903 ± 0.224
1.313MetArg: 1.313 ± 0.295
2.79MetSer: 2.79 ± 0.51
1.641MetThr: 1.641 ± 0.415
1.641MetVal: 1.641 ± 0.345
0.41MetTrp: 0.41 ± 0.168
0.821MetTyr: 0.821 ± 0.279
0.0MetXaa: 0.0 ± 0.0
Asn
4.513AsnAla: 4.513 ± 0.655
0.246AsnCys: 0.246 ± 0.143
4.349AsnAsp: 4.349 ± 0.639
5.744AsnGlu: 5.744 ± 1.165
1.559AsnPhe: 1.559 ± 0.628
5.17AsnGly: 5.17 ± 0.611
0.903AsnHis: 0.903 ± 0.247
4.349AsnIle: 4.349 ± 0.619
6.729AsnLys: 6.729 ± 0.878
4.185AsnLeu: 4.185 ± 0.673
1.395AsnMet: 1.395 ± 0.319
4.267AsnAsn: 4.267 ± 0.7
2.462AsnPro: 2.462 ± 0.377
2.462AsnGln: 2.462 ± 0.445
3.447AsnArg: 3.447 ± 0.598
3.282AsnSer: 3.282 ± 0.482
3.365AsnThr: 3.365 ± 0.512
3.447AsnVal: 3.447 ± 0.549
0.985AsnTrp: 0.985 ± 0.373
2.544AsnTyr: 2.544 ± 0.398
0.0AsnXaa: 0.0 ± 0.0
Pro
1.149ProAla: 1.149 ± 0.315
0.0ProCys: 0.0 ± 0.0
1.231ProAsp: 1.231 ± 0.294
2.216ProGlu: 2.216 ± 0.388
1.231ProPhe: 1.231 ± 0.295
1.149ProGly: 1.149 ± 0.296
0.574ProHis: 0.574 ± 0.182
2.708ProIle: 2.708 ± 0.393
2.79ProLys: 2.79 ± 0.584
1.887ProLeu: 1.887 ± 0.395
0.739ProMet: 0.739 ± 0.199
1.149ProAsn: 1.149 ± 0.289
0.985ProPro: 0.985 ± 0.261
0.903ProGln: 0.903 ± 0.283
1.149ProArg: 1.149 ± 0.279
1.887ProSer: 1.887 ± 0.469
1.559ProThr: 1.559 ± 0.431
1.477ProVal: 1.477 ± 0.362
0.164ProTrp: 0.164 ± 0.105
1.313ProTyr: 1.313 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
2.79GlnAla: 2.79 ± 0.469
0.41GlnCys: 0.41 ± 0.212
1.559GlnAsp: 1.559 ± 0.423
3.2GlnGlu: 3.2 ± 0.637
1.149GlnPhe: 1.149 ± 0.276
2.134GlnGly: 2.134 ± 0.432
0.903GlnHis: 0.903 ± 0.269
2.872GlnIle: 2.872 ± 0.524
3.529GlnLys: 3.529 ± 0.588
2.462GlnLeu: 2.462 ± 0.386
0.574GlnMet: 0.574 ± 0.216
2.38GlnAsn: 2.38 ± 0.452
1.067GlnPro: 1.067 ± 0.289
1.395GlnGln: 1.395 ± 0.361
1.641GlnArg: 1.641 ± 0.317
1.805GlnSer: 1.805 ± 0.376
1.395GlnThr: 1.395 ± 0.321
2.134GlnVal: 2.134 ± 0.459
0.328GlnTrp: 0.328 ± 0.164
1.559GlnTyr: 1.559 ± 0.298
0.0GlnXaa: 0.0 ± 0.0
Arg
2.462ArgAla: 2.462 ± 0.393
0.246ArgCys: 0.246 ± 0.166
2.954ArgAsp: 2.954 ± 0.562
3.857ArgGlu: 3.857 ± 0.626
1.805ArgPhe: 1.805 ± 0.386
1.805ArgGly: 1.805 ± 0.436
0.574ArgHis: 0.574 ± 0.22
3.118ArgIle: 3.118 ± 0.551
4.103ArgLys: 4.103 ± 0.726
4.185ArgLeu: 4.185 ± 0.57
1.477ArgMet: 1.477 ± 0.371
2.872ArgAsn: 2.872 ± 0.404
0.821ArgPro: 0.821 ± 0.216
1.231ArgGln: 1.231 ± 0.341
2.298ArgArg: 2.298 ± 0.465
1.313ArgSer: 1.313 ± 0.309
2.216ArgThr: 2.216 ± 0.458
2.298ArgVal: 2.298 ± 0.414
0.492ArgTrp: 0.492 ± 0.207
2.872ArgTyr: 2.872 ± 0.715
0.0ArgXaa: 0.0 ± 0.0
Ser
3.939SerAla: 3.939 ± 0.611
0.41SerCys: 0.41 ± 0.182
4.595SerAsp: 4.595 ± 0.859
5.826SerGlu: 5.826 ± 0.676
3.282SerPhe: 3.282 ± 0.652
3.2SerGly: 3.2 ± 0.873
1.477SerHis: 1.477 ± 0.254
5.416SerIle: 5.416 ± 0.894
4.842SerLys: 4.842 ± 0.58
3.775SerLeu: 3.775 ± 0.6
1.067SerMet: 1.067 ± 0.248
4.349SerAsn: 4.349 ± 0.614
0.739SerPro: 0.739 ± 0.202
1.559SerGln: 1.559 ± 0.375
1.723SerArg: 1.723 ± 0.346
3.282SerSer: 3.282 ± 0.512
3.036SerThr: 3.036 ± 0.519
2.462SerVal: 2.462 ± 0.479
0.246SerTrp: 0.246 ± 0.139
2.216SerTyr: 2.216 ± 0.433
0.0SerXaa: 0.0 ± 0.0
Thr
2.79ThrAla: 2.79 ± 0.547
0.082ThrCys: 0.082 ± 0.085
3.857ThrAsp: 3.857 ± 0.807
3.857ThrGlu: 3.857 ± 0.499
2.216ThrPhe: 2.216 ± 0.504
3.693ThrGly: 3.693 ± 0.737
1.313ThrHis: 1.313 ± 0.272
4.513ThrIle: 4.513 ± 0.672
5.088ThrLys: 5.088 ± 0.625
4.021ThrLeu: 4.021 ± 0.42
0.903ThrMet: 0.903 ± 0.221
3.282ThrAsn: 3.282 ± 0.473
1.723ThrPro: 1.723 ± 0.328
1.149ThrGln: 1.149 ± 0.359
2.216ThrArg: 2.216 ± 0.494
3.118ThrSer: 3.118 ± 0.574
3.036ThrThr: 3.036 ± 0.61
3.693ThrVal: 3.693 ± 0.579
0.739ThrTrp: 0.739 ± 0.213
3.036ThrTyr: 3.036 ± 0.522
0.0ThrXaa: 0.0 ± 0.0
Val
2.954ValAla: 2.954 ± 0.55
0.082ValCys: 0.082 ± 0.096
3.365ValAsp: 3.365 ± 0.572
4.021ValGlu: 4.021 ± 0.855
2.216ValPhe: 2.216 ± 0.463
3.118ValGly: 3.118 ± 0.65
0.574ValHis: 0.574 ± 0.18
5.334ValIle: 5.334 ± 0.653
5.908ValLys: 5.908 ± 0.526
4.76ValLeu: 4.76 ± 0.496
1.887ValMet: 1.887 ± 0.405
4.021ValAsn: 4.021 ± 0.43
1.313ValPro: 1.313 ± 0.33
2.052ValGln: 2.052 ± 0.581
2.216ValArg: 2.216 ± 0.395
2.872ValSer: 2.872 ± 0.497
3.611ValThr: 3.611 ± 0.574
3.611ValVal: 3.611 ± 0.645
0.574ValTrp: 0.574 ± 0.226
2.954ValTyr: 2.954 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
0.41TrpAla: 0.41 ± 0.171
0.082TrpCys: 0.082 ± 0.069
0.821TrpAsp: 0.821 ± 0.282
1.067TrpGlu: 1.067 ± 0.312
0.574TrpPhe: 0.574 ± 0.193
0.656TrpGly: 0.656 ± 0.181
0.082TrpHis: 0.082 ± 0.069
1.313TrpIle: 1.313 ± 0.289
0.903TrpLys: 0.903 ± 0.248
0.656TrpLeu: 0.656 ± 0.227
0.574TrpMet: 0.574 ± 0.207
0.656TrpAsn: 0.656 ± 0.283
0.082TrpPro: 0.082 ± 0.067
0.574TrpGln: 0.574 ± 0.169
0.492TrpArg: 0.492 ± 0.168
0.574TrpSer: 0.574 ± 0.281
0.328TrpThr: 0.328 ± 0.117
0.656TrpVal: 0.656 ± 0.203
0.164TrpTrp: 0.164 ± 0.089
0.492TrpTyr: 0.492 ± 0.173
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.887TyrAla: 1.887 ± 0.343
0.164TyrCys: 0.164 ± 0.118
3.282TyrAsp: 3.282 ± 0.613
4.021TyrGlu: 4.021 ± 0.762
2.38TyrPhe: 2.38 ± 0.544
2.626TyrGly: 2.626 ± 0.49
0.656TyrHis: 0.656 ± 0.219
3.939TyrIle: 3.939 ± 0.799
5.17TyrLys: 5.17 ± 0.766
3.693TyrLeu: 3.693 ± 0.633
1.067TyrMet: 1.067 ± 0.311
2.708TyrAsn: 2.708 ± 0.396
0.985TyrPro: 0.985 ± 0.281
2.544TyrGln: 2.544 ± 0.435
2.134TyrArg: 2.134 ± 0.529
2.626TyrSer: 2.626 ± 0.505
2.298TyrThr: 2.298 ± 0.476
2.544TyrVal: 2.544 ± 0.51
0.656TyrTrp: 0.656 ± 0.217
1.149TyrTyr: 1.149 ± 0.385
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (12187 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski