Rosellinia necatrix partitivirus 8
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5WQE2|A0A2Z5WQE2_9VIRU Coat protein OS=Rosellinia necatrix partitivirus 8 OX=2025333 PE=4 SV=1
MM1 pKa = 7.27 LTLFTIVTLNILLRR15 pKa = 11.84 FQQRR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 NEE24 pKa = 3.77 TTSTLIHH31 pKa = 7.26 PIRR34 pKa = 11.84 NILFEE39 pKa = 4.14 WEE41 pKa = 4.17 FRR43 pKa = 11.84 RR44 pKa = 11.84 SIPLDD49 pKa = 3.52 ASPYY53 pKa = 9.98 PVFDD57 pKa = 3.65 YY58 pKa = 11.13 SKK60 pKa = 11.3 SLLAYY65 pKa = 9.29 IDD67 pKa = 5.96 LITCHH72 pKa = 6.95 KK73 pKa = 10.37 ISIHH77 pKa = 5.76 LKK79 pKa = 8.19 EE80 pKa = 5.45 DD81 pKa = 3.06 IYY83 pKa = 11.09 TLEE86 pKa = 3.9 EE87 pKa = 4.45 RR88 pKa = 11.84 YY89 pKa = 10.06 SSRR92 pKa = 11.84 DD93 pKa = 3.54 EE94 pKa = 4.32 NFHH97 pKa = 7.4 LYY99 pKa = 10.37 EE100 pKa = 4.75 YY101 pKa = 9.62 ITDD104 pKa = 3.94 ADD106 pKa = 4.57 LPPDD110 pKa = 3.76 RR111 pKa = 11.84 QPANGISHH119 pKa = 7.29 AEE121 pKa = 3.87 RR122 pKa = 11.84 RR123 pKa = 11.84 YY124 pKa = 10.16 HH125 pKa = 6.86 SIPSGSLNVNDD136 pKa = 4.8 KK137 pKa = 11.12 IEE139 pKa = 4.48 VISNDD144 pKa = 3.17 PDD146 pKa = 4.33 FIEE149 pKa = 4.45 TNEE152 pKa = 3.79 FRR154 pKa = 11.84 SNILFAEE161 pKa = 4.28 SLDD164 pKa = 3.93 LTGSPPHH171 pKa = 5.98 PQIEE175 pKa = 4.62 KK176 pKa = 10.62 LIQDD180 pKa = 4.4 WFPQYY185 pKa = 11.08 VPYY188 pKa = 10.52 LHH190 pKa = 7.18 EE191 pKa = 4.18 YY192 pKa = 9.58 CRR194 pKa = 11.84 PPSFGPQAFLDD205 pKa = 4.29 FNRR208 pKa = 11.84 DD209 pKa = 3.35 TPDD212 pKa = 3.78 PQPPTPEE219 pKa = 3.39 RR220 pKa = 11.84 HH221 pKa = 4.71 EE222 pKa = 4.64 AIMRR226 pKa = 11.84 IVRR229 pKa = 11.84 LKK231 pKa = 10.73 MNIKK235 pKa = 9.99 PYY237 pKa = 10.32 RR238 pKa = 11.84 PLHH241 pKa = 5.77 FADD244 pKa = 4.26 ALAAEE249 pKa = 4.81 TPLNTSASYY258 pKa = 10.43 YY259 pKa = 10.74 SKK261 pKa = 10.79 FNPEE265 pKa = 3.44 TRR267 pKa = 11.84 VLARR271 pKa = 11.84 YY272 pKa = 7.4 STPSRR277 pKa = 11.84 YY278 pKa = 10.09 SDD280 pKa = 3.13 MPTSKK285 pKa = 10.81 GYY287 pKa = 10.45 FINVMLNEE295 pKa = 3.9 FRR297 pKa = 11.84 LEE299 pKa = 3.91 FHH301 pKa = 6.88 HH302 pKa = 6.85 IKK304 pKa = 10.15 YY305 pKa = 10.53 DD306 pKa = 3.6 GMPFPTDD313 pKa = 3.06 RR314 pKa = 11.84 HH315 pKa = 5.19 DD316 pKa = 4.02 HH317 pKa = 5.35 EE318 pKa = 5.12 TNLTILDD325 pKa = 3.49 TWLAKK330 pKa = 10.5 HH331 pKa = 6.27 PAQLFIRR338 pKa = 11.84 TQISKK343 pKa = 10.3 RR344 pKa = 11.84 DD345 pKa = 3.39 PSDD348 pKa = 3.26 PKK350 pKa = 10.5 KK351 pKa = 10.23 IRR353 pKa = 11.84 PVYY356 pKa = 10.59 SVDD359 pKa = 4.16 DD360 pKa = 3.94 RR361 pKa = 11.84 FLHH364 pKa = 6.15 IEE366 pKa = 4.2 KK367 pKa = 9.54 TVSTPLLAQMRR378 pKa = 11.84 NPQCCVAHH386 pKa = 5.99 GLEE389 pKa = 4.28 TFRR392 pKa = 11.84 GSMSLIDD399 pKa = 4.4 KK400 pKa = 9.63 IAHH403 pKa = 6.37 FFLSFISLDD412 pKa = 2.89 WSQFDD417 pKa = 3.23 QRR419 pKa = 11.84 LPYY422 pKa = 10.14 YY423 pKa = 10.23 VIIAFFLDD431 pKa = 4.4 FLASLIIVSHH441 pKa = 6.7 GYY443 pKa = 8.53 MPTRR447 pKa = 11.84 SYY449 pKa = 11.39 PDD451 pKa = 3.37 TKK453 pKa = 11.21 SDD455 pKa = 2.69 IHH457 pKa = 7.82 SFARR461 pKa = 11.84 RR462 pKa = 11.84 QYY464 pKa = 9.77 NVLVFLTTWYY474 pKa = 11.15 LSMTFLSFDD483 pKa = 3.03 GFAFIRR489 pKa = 11.84 IHH491 pKa = 6.33 GGVPSGLLNTQSLDD505 pKa = 3.37 SFGNMYY511 pKa = 10.44 IITDD515 pKa = 3.8 CLLEE519 pKa = 4.23 FGFTEE524 pKa = 4.41 QEE526 pKa = 4.07 CLEE529 pKa = 4.16 MLFCVLGDD537 pKa = 3.91 DD538 pKa = 4.4 NLIFLQHH545 pKa = 5.62 NLEE548 pKa = 4.78 RR549 pKa = 11.84 ITRR552 pKa = 11.84 FMVFLEE558 pKa = 4.63 RR559 pKa = 11.84 YY560 pKa = 8.88 SKK562 pKa = 10.55 DD563 pKa = 2.83 RR564 pKa = 11.84 HH565 pKa = 5.9 GMVLSILKK573 pKa = 10.83 SMFSNLRR580 pKa = 11.84 TKK582 pKa = 9.21 ITFLSYY588 pKa = 11.31 EE589 pKa = 4.09 NFFGHH594 pKa = 7.23 PSRR597 pKa = 11.84 PIGKK601 pKa = 9.18 LVAQLAFPEE610 pKa = 4.64 RR611 pKa = 11.84 PVPPARR617 pKa = 11.84 EE618 pKa = 4.11 WIHH621 pKa = 6.13 AARR624 pKa = 11.84 ALGLAYY630 pKa = 9.97 ASCGQDD636 pKa = 3.01 PTFHH640 pKa = 7.23 LLCKK644 pKa = 9.53 MVYY647 pKa = 9.14 EE648 pKa = 4.55 RR649 pKa = 11.84 FRR651 pKa = 11.84 PSVPVPTLHH660 pKa = 6.77 INKK663 pKa = 9.03 IFKK666 pKa = 8.89 KK667 pKa = 9.44 WKK669 pKa = 8.48 YY670 pKa = 9.07 QLPDD674 pKa = 3.53 FDD676 pKa = 5.05 IEE678 pKa = 4.12 EE679 pKa = 4.44 LEE681 pKa = 4.24 YY682 pKa = 10.74 TFPDD686 pKa = 4.69 FPTCTEE692 pKa = 3.67 IFALVSDD699 pKa = 3.82 YY700 pKa = 11.39 HH701 pKa = 8.12 GVFSEE706 pKa = 4.09 TDD708 pKa = 2.5 KK709 pKa = 11.33 WNFNVFTVPPSDD721 pKa = 3.88 NLPDD725 pKa = 3.67 YY726 pKa = 9.74 VTLKK730 pKa = 10.96 NYY732 pKa = 9.15 IQQSPDD738 pKa = 2.74 VSHH741 pKa = 6.47 TVNEE745 pKa = 4.48 FWHH748 pKa = 6.46 GKK750 pKa = 9.82 RR751 pKa = 11.84 PFF753 pKa = 3.6
Molecular weight: 88.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.973
IPC2_protein 5.906
IPC_protein 6.008
Toseland 6.478
ProMoST 6.402
Dawson 6.326
Bjellqvist 6.326
Wikipedia 6.351
Rodwell 6.326
Grimsley 6.649
Solomon 6.326
Lehninger 6.326
Nozaki 6.62
DTASelect 6.81
Thurlkill 6.825
EMBOSS 6.795
Sillero 6.722
Patrickios 3.973
IPC_peptide 6.351
IPC2_peptide 6.693
IPC2.peptide.svr19 6.631
Protein with the highest isoelectric point:
>tr|A0A2Z5WQE2|A0A2Z5WQE2_9VIRU Coat protein OS=Rosellinia necatrix partitivirus 8 OX=2025333 PE=4 SV=1
MM1 pKa = 7.38 SSSRR5 pKa = 11.84 LRR7 pKa = 11.84 STIPAPAAPTVTVQDD22 pKa = 4.1 GQPAAPASVAPQVLQYY38 pKa = 11.03 LDD40 pKa = 3.65 PSRR43 pKa = 11.84 NTDD46 pKa = 3.2 SNTGILFNLEE56 pKa = 3.91 WVPDD60 pKa = 4.23 FRR62 pKa = 11.84 FMLLSWLYY70 pKa = 9.1 SLARR74 pKa = 11.84 IVPNSAYY81 pKa = 9.36 TSSPYY86 pKa = 10.78 ASPASSIGYY95 pKa = 7.32 TIVMLVAFLYY105 pKa = 8.91 HH106 pKa = 6.63 TDD108 pKa = 3.58 ASHH111 pKa = 6.56 LQHH114 pKa = 6.93 PSAAASAIMNDD125 pKa = 3.14 ALFSRR130 pKa = 11.84 FFDD133 pKa = 3.47 MLLDD137 pKa = 3.97 FPVPDD142 pKa = 4.08 FASNEE147 pKa = 4.15 FASLMAFLPDD157 pKa = 5.52 DD158 pKa = 3.87 IPNLVILTSLASADD172 pKa = 3.86 YY173 pKa = 8.57 YY174 pKa = 11.3 HH175 pKa = 7.78 DD176 pKa = 4.09 FGRR179 pKa = 11.84 HH180 pKa = 4.02 FSANIFFLAHH190 pKa = 6.42 NLLAGLPANTPTSTLRR206 pKa = 11.84 ATFYY210 pKa = 8.6 ATPVNSVNIGNNNNVNITPGQLFGRR235 pKa = 11.84 INGANTVSNWLNQRR249 pKa = 11.84 IDD251 pKa = 3.32 AMINSMAIRR260 pKa = 11.84 AVNQNNMVAQIQFPTVALANTANYY284 pKa = 10.33 NPYY287 pKa = 10.67 LFLASLDD294 pKa = 3.43 QHH296 pKa = 6.85 NIGSITSAMRR306 pKa = 11.84 NMSQWISATFPASKK320 pKa = 9.33 TLRR323 pKa = 11.84 HH324 pKa = 5.27 YY325 pKa = 8.9 MQAGSHH331 pKa = 5.01 EE332 pKa = 4.17 TSNYY336 pKa = 9.7 LFAEE340 pKa = 4.29 IALPTWNTTQITITVPVSQTNSDD363 pKa = 3.47 KK364 pKa = 10.79 FLPTAAPDD372 pKa = 3.5 QNATEE377 pKa = 4.41 LAHH380 pKa = 7.46 DD381 pKa = 4.24 SGFLTVPTAPADD393 pKa = 3.54 TDD395 pKa = 3.96 VNGTSVWNIAAEE407 pKa = 4.44 RR408 pKa = 11.84 AASTPPANPMYY419 pKa = 10.92 ASLRR423 pKa = 11.84 SSATRR428 pKa = 11.84 PDD430 pKa = 4.22 PYY432 pKa = 10.93 VNPIPHH438 pKa = 5.48 VTFDD442 pKa = 3.81 VNLHH446 pKa = 5.19 VLPRR450 pKa = 11.84 MYY452 pKa = 10.18 IYY454 pKa = 10.88 APYY457 pKa = 8.5 VTNTGALGSVITSGKK472 pKa = 10.0 LIEE475 pKa = 5.05 SGDD478 pKa = 3.4 ISGIMQIVPSTTTPLLYY495 pKa = 10.6 EE496 pKa = 4.04 NSQFHH501 pKa = 7.33 DD502 pKa = 3.86 GAIRR506 pKa = 11.84 LSRR509 pKa = 11.84 TRR511 pKa = 11.84 SALFHH516 pKa = 6.84 AGIQIHH522 pKa = 4.94 MQEE525 pKa = 3.99 RR526 pKa = 11.84 PARR529 pKa = 11.84 RR530 pKa = 11.84 TLDD533 pKa = 3.21 APIAFFRR540 pKa = 11.84 GLTSGLRR547 pKa = 11.84 YY548 pKa = 8.53 PVPSTGPVYY557 pKa = 10.73 APSAFPNSSQARR569 pKa = 11.84 LLPGARR575 pKa = 11.84 WTSHH579 pKa = 4.42 ATNFFHH585 pKa = 6.7 TLNVFGRR592 pKa = 11.84 DD593 pKa = 3.54 LSHH596 pKa = 7.8 DD597 pKa = 4.08 LQLHH601 pKa = 6.37 ADD603 pKa = 3.4 SSFNIWSGLRR613 pKa = 11.84 FRR615 pKa = 11.84 YY616 pKa = 6.75 MTNTGPVVYY625 pKa = 9.4 VLPSARR631 pKa = 11.84 HH632 pKa = 4.68 IFGARR637 pKa = 11.84 ARR639 pKa = 11.84 SYY641 pKa = 9.58 GTEE644 pKa = 3.97 HH645 pKa = 7.07 PALRR649 pKa = 11.84 LPLL652 pKa = 4.15
Molecular weight: 71.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.795
IPC2_protein 6.59
IPC_protein 6.942
Toseland 7.527
ProMoST 7.22
Dawson 7.249
Bjellqvist 7.088
Wikipedia 7.293
Rodwell 7.234
Grimsley 7.702
Solomon 7.293
Lehninger 7.293
Nozaki 7.395
DTASelect 7.644
Thurlkill 7.658
EMBOSS 7.717
Sillero 7.585
Patrickios 3.948
IPC_peptide 7.307
IPC2_peptide 7.395
IPC2.peptide.svr19 7.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1405
652
753
702.5
79.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.616 ± 2.646
0.712 ± 0.485
5.338 ± 0.71
3.559 ± 1.378
6.69 ± 0.9
3.701 ± 0.508
3.63 ± 0.278
6.335 ± 0.553
2.278 ± 1.237
9.68 ± 0.325
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.349 ± 0.176
5.48 ± 1.072
7.829 ± 0.1
3.203 ± 0.117
5.836 ± 0.423
8.541 ± 0.868
7.046 ± 0.737
4.769 ± 0.408
1.21 ± 0.012
4.199 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here