Rosellinia necatrix partitivirus 8

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2Z5WQE2|A0A2Z5WQE2_9VIRU Coat protein OS=Rosellinia necatrix partitivirus 8 OX=2025333 PE=4 SV=1
MM1 pKa = 7.27LTLFTIVTLNILLRR15 pKa = 11.84FQQRR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84NEE24 pKa = 3.77TTSTLIHH31 pKa = 7.26PIRR34 pKa = 11.84NILFEE39 pKa = 4.14WEE41 pKa = 4.17FRR43 pKa = 11.84RR44 pKa = 11.84SIPLDD49 pKa = 3.52ASPYY53 pKa = 9.98PVFDD57 pKa = 3.65YY58 pKa = 11.13SKK60 pKa = 11.3SLLAYY65 pKa = 9.29IDD67 pKa = 5.96LITCHH72 pKa = 6.95KK73 pKa = 10.37ISIHH77 pKa = 5.76LKK79 pKa = 8.19EE80 pKa = 5.45DD81 pKa = 3.06IYY83 pKa = 11.09TLEE86 pKa = 3.9EE87 pKa = 4.45RR88 pKa = 11.84YY89 pKa = 10.06SSRR92 pKa = 11.84DD93 pKa = 3.54EE94 pKa = 4.32NFHH97 pKa = 7.4LYY99 pKa = 10.37EE100 pKa = 4.75YY101 pKa = 9.62ITDD104 pKa = 3.94ADD106 pKa = 4.57LPPDD110 pKa = 3.76RR111 pKa = 11.84QPANGISHH119 pKa = 7.29AEE121 pKa = 3.87RR122 pKa = 11.84RR123 pKa = 11.84YY124 pKa = 10.16HH125 pKa = 6.86SIPSGSLNVNDD136 pKa = 4.8KK137 pKa = 11.12IEE139 pKa = 4.48VISNDD144 pKa = 3.17PDD146 pKa = 4.33FIEE149 pKa = 4.45TNEE152 pKa = 3.79FRR154 pKa = 11.84SNILFAEE161 pKa = 4.28SLDD164 pKa = 3.93LTGSPPHH171 pKa = 5.98PQIEE175 pKa = 4.62KK176 pKa = 10.62LIQDD180 pKa = 4.4WFPQYY185 pKa = 11.08VPYY188 pKa = 10.52LHH190 pKa = 7.18EE191 pKa = 4.18YY192 pKa = 9.58CRR194 pKa = 11.84PPSFGPQAFLDD205 pKa = 4.29FNRR208 pKa = 11.84DD209 pKa = 3.35TPDD212 pKa = 3.78PQPPTPEE219 pKa = 3.39RR220 pKa = 11.84HH221 pKa = 4.71EE222 pKa = 4.64AIMRR226 pKa = 11.84IVRR229 pKa = 11.84LKK231 pKa = 10.73MNIKK235 pKa = 9.99PYY237 pKa = 10.32RR238 pKa = 11.84PLHH241 pKa = 5.77FADD244 pKa = 4.26ALAAEE249 pKa = 4.81TPLNTSASYY258 pKa = 10.43YY259 pKa = 10.74SKK261 pKa = 10.79FNPEE265 pKa = 3.44TRR267 pKa = 11.84VLARR271 pKa = 11.84YY272 pKa = 7.4STPSRR277 pKa = 11.84YY278 pKa = 10.09SDD280 pKa = 3.13MPTSKK285 pKa = 10.81GYY287 pKa = 10.45FINVMLNEE295 pKa = 3.9FRR297 pKa = 11.84LEE299 pKa = 3.91FHH301 pKa = 6.88HH302 pKa = 6.85IKK304 pKa = 10.15YY305 pKa = 10.53DD306 pKa = 3.6GMPFPTDD313 pKa = 3.06RR314 pKa = 11.84HH315 pKa = 5.19DD316 pKa = 4.02HH317 pKa = 5.35EE318 pKa = 5.12TNLTILDD325 pKa = 3.49TWLAKK330 pKa = 10.5HH331 pKa = 6.27PAQLFIRR338 pKa = 11.84TQISKK343 pKa = 10.3RR344 pKa = 11.84DD345 pKa = 3.39PSDD348 pKa = 3.26PKK350 pKa = 10.5KK351 pKa = 10.23IRR353 pKa = 11.84PVYY356 pKa = 10.59SVDD359 pKa = 4.16DD360 pKa = 3.94RR361 pKa = 11.84FLHH364 pKa = 6.15IEE366 pKa = 4.2KK367 pKa = 9.54TVSTPLLAQMRR378 pKa = 11.84NPQCCVAHH386 pKa = 5.99GLEE389 pKa = 4.28TFRR392 pKa = 11.84GSMSLIDD399 pKa = 4.4KK400 pKa = 9.63IAHH403 pKa = 6.37FFLSFISLDD412 pKa = 2.89WSQFDD417 pKa = 3.23QRR419 pKa = 11.84LPYY422 pKa = 10.14YY423 pKa = 10.23VIIAFFLDD431 pKa = 4.4FLASLIIVSHH441 pKa = 6.7GYY443 pKa = 8.53MPTRR447 pKa = 11.84SYY449 pKa = 11.39PDD451 pKa = 3.37TKK453 pKa = 11.21SDD455 pKa = 2.69IHH457 pKa = 7.82SFARR461 pKa = 11.84RR462 pKa = 11.84QYY464 pKa = 9.77NVLVFLTTWYY474 pKa = 11.15LSMTFLSFDD483 pKa = 3.03GFAFIRR489 pKa = 11.84IHH491 pKa = 6.33GGVPSGLLNTQSLDD505 pKa = 3.37SFGNMYY511 pKa = 10.44IITDD515 pKa = 3.8CLLEE519 pKa = 4.23FGFTEE524 pKa = 4.41QEE526 pKa = 4.07CLEE529 pKa = 4.16MLFCVLGDD537 pKa = 3.91DD538 pKa = 4.4NLIFLQHH545 pKa = 5.62NLEE548 pKa = 4.78RR549 pKa = 11.84ITRR552 pKa = 11.84FMVFLEE558 pKa = 4.63RR559 pKa = 11.84YY560 pKa = 8.88SKK562 pKa = 10.55DD563 pKa = 2.83RR564 pKa = 11.84HH565 pKa = 5.9GMVLSILKK573 pKa = 10.83SMFSNLRR580 pKa = 11.84TKK582 pKa = 9.21ITFLSYY588 pKa = 11.31EE589 pKa = 4.09NFFGHH594 pKa = 7.23PSRR597 pKa = 11.84PIGKK601 pKa = 9.18LVAQLAFPEE610 pKa = 4.64RR611 pKa = 11.84PVPPARR617 pKa = 11.84EE618 pKa = 4.11WIHH621 pKa = 6.13AARR624 pKa = 11.84ALGLAYY630 pKa = 9.97ASCGQDD636 pKa = 3.01PTFHH640 pKa = 7.23LLCKK644 pKa = 9.53MVYY647 pKa = 9.14EE648 pKa = 4.55RR649 pKa = 11.84FRR651 pKa = 11.84PSVPVPTLHH660 pKa = 6.77INKK663 pKa = 9.03IFKK666 pKa = 8.89KK667 pKa = 9.44WKK669 pKa = 8.48YY670 pKa = 9.07QLPDD674 pKa = 3.53FDD676 pKa = 5.05IEE678 pKa = 4.12EE679 pKa = 4.44LEE681 pKa = 4.24YY682 pKa = 10.74TFPDD686 pKa = 4.69FPTCTEE692 pKa = 3.67IFALVSDD699 pKa = 3.82YY700 pKa = 11.39HH701 pKa = 8.12GVFSEE706 pKa = 4.09TDD708 pKa = 2.5KK709 pKa = 11.33WNFNVFTVPPSDD721 pKa = 3.88NLPDD725 pKa = 3.67YY726 pKa = 9.74VTLKK730 pKa = 10.96NYY732 pKa = 9.15IQQSPDD738 pKa = 2.74VSHH741 pKa = 6.47TVNEE745 pKa = 4.48FWHH748 pKa = 6.46GKK750 pKa = 9.82RR751 pKa = 11.84PFF753 pKa = 3.6

Molecular weight:
88.12 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2Z5WQE2|A0A2Z5WQE2_9VIRU Coat protein OS=Rosellinia necatrix partitivirus 8 OX=2025333 PE=4 SV=1
MM1 pKa = 7.38SSSRR5 pKa = 11.84LRR7 pKa = 11.84STIPAPAAPTVTVQDD22 pKa = 4.1GQPAAPASVAPQVLQYY38 pKa = 11.03LDD40 pKa = 3.65PSRR43 pKa = 11.84NTDD46 pKa = 3.2SNTGILFNLEE56 pKa = 3.91WVPDD60 pKa = 4.23FRR62 pKa = 11.84FMLLSWLYY70 pKa = 9.1SLARR74 pKa = 11.84IVPNSAYY81 pKa = 9.36TSSPYY86 pKa = 10.78ASPASSIGYY95 pKa = 7.32TIVMLVAFLYY105 pKa = 8.91HH106 pKa = 6.63TDD108 pKa = 3.58ASHH111 pKa = 6.56LQHH114 pKa = 6.93PSAAASAIMNDD125 pKa = 3.14ALFSRR130 pKa = 11.84FFDD133 pKa = 3.47MLLDD137 pKa = 3.97FPVPDD142 pKa = 4.08FASNEE147 pKa = 4.15FASLMAFLPDD157 pKa = 5.52DD158 pKa = 3.87IPNLVILTSLASADD172 pKa = 3.86YY173 pKa = 8.57YY174 pKa = 11.3HH175 pKa = 7.78DD176 pKa = 4.09FGRR179 pKa = 11.84HH180 pKa = 4.02FSANIFFLAHH190 pKa = 6.42NLLAGLPANTPTSTLRR206 pKa = 11.84ATFYY210 pKa = 8.6ATPVNSVNIGNNNNVNITPGQLFGRR235 pKa = 11.84INGANTVSNWLNQRR249 pKa = 11.84IDD251 pKa = 3.32AMINSMAIRR260 pKa = 11.84AVNQNNMVAQIQFPTVALANTANYY284 pKa = 10.33NPYY287 pKa = 10.67LFLASLDD294 pKa = 3.43QHH296 pKa = 6.85NIGSITSAMRR306 pKa = 11.84NMSQWISATFPASKK320 pKa = 9.33TLRR323 pKa = 11.84HH324 pKa = 5.27YY325 pKa = 8.9MQAGSHH331 pKa = 5.01EE332 pKa = 4.17TSNYY336 pKa = 9.7LFAEE340 pKa = 4.29IALPTWNTTQITITVPVSQTNSDD363 pKa = 3.47KK364 pKa = 10.79FLPTAAPDD372 pKa = 3.5QNATEE377 pKa = 4.41LAHH380 pKa = 7.46DD381 pKa = 4.24SGFLTVPTAPADD393 pKa = 3.54TDD395 pKa = 3.96VNGTSVWNIAAEE407 pKa = 4.44RR408 pKa = 11.84AASTPPANPMYY419 pKa = 10.92ASLRR423 pKa = 11.84SSATRR428 pKa = 11.84PDD430 pKa = 4.22PYY432 pKa = 10.93VNPIPHH438 pKa = 5.48VTFDD442 pKa = 3.81VNLHH446 pKa = 5.19VLPRR450 pKa = 11.84MYY452 pKa = 10.18IYY454 pKa = 10.88APYY457 pKa = 8.5VTNTGALGSVITSGKK472 pKa = 10.0LIEE475 pKa = 5.05SGDD478 pKa = 3.4ISGIMQIVPSTTTPLLYY495 pKa = 10.6EE496 pKa = 4.04NSQFHH501 pKa = 7.33DD502 pKa = 3.86GAIRR506 pKa = 11.84LSRR509 pKa = 11.84TRR511 pKa = 11.84SALFHH516 pKa = 6.84AGIQIHH522 pKa = 4.94MQEE525 pKa = 3.99RR526 pKa = 11.84PARR529 pKa = 11.84RR530 pKa = 11.84TLDD533 pKa = 3.21APIAFFRR540 pKa = 11.84GLTSGLRR547 pKa = 11.84YY548 pKa = 8.53PVPSTGPVYY557 pKa = 10.73APSAFPNSSQARR569 pKa = 11.84LLPGARR575 pKa = 11.84WTSHH579 pKa = 4.42ATNFFHH585 pKa = 6.7TLNVFGRR592 pKa = 11.84DD593 pKa = 3.54LSHH596 pKa = 7.8DD597 pKa = 4.08LQLHH601 pKa = 6.37ADD603 pKa = 3.4SSFNIWSGLRR613 pKa = 11.84FRR615 pKa = 11.84YY616 pKa = 6.75MTNTGPVVYY625 pKa = 9.4VLPSARR631 pKa = 11.84HH632 pKa = 4.68IFGARR637 pKa = 11.84ARR639 pKa = 11.84SYY641 pKa = 9.58GTEE644 pKa = 3.97HH645 pKa = 7.07PALRR649 pKa = 11.84LPLL652 pKa = 4.15

Molecular weight:
71.26 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1405

652

753

702.5

79.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.616 ± 2.646

0.712 ± 0.485

5.338 ± 0.71

3.559 ± 1.378

6.69 ± 0.9

3.701 ± 0.508

3.63 ± 0.278

6.335 ± 0.553

2.278 ± 1.237

9.68 ± 0.325

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.176

5.48 ± 1.072

7.829 ± 0.1

3.203 ± 0.117

5.836 ± 0.423

8.541 ± 0.868

7.046 ± 0.737

4.769 ± 0.408

1.21 ± 0.012

4.199 ± 0.353

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski