Escherichia phage Lilleen

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; unclassified Bullavirinae

Average proteome isoelectric point is 7.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6TCG9|A0A4D6TCG9_9VIRU External scaffolding protein D OS=Escherichia phage Lilleen OX=2562233 PE=3 SV=1
MM1 pKa = 7.53NSNEE5 pKa = 4.22SAVAFQTAIASIKK18 pKa = 9.2MIQASSVLDD27 pKa = 3.53LTEE30 pKa = 5.82DD31 pKa = 3.47DD32 pKa = 5.78FEE34 pKa = 4.82FLTGDD39 pKa = 3.9RR40 pKa = 11.84VWIATDD46 pKa = 3.49RR47 pKa = 11.84SRR49 pKa = 11.84ARR51 pKa = 11.84RR52 pKa = 11.84AVEE55 pKa = 3.52ACVYY59 pKa = 8.38GTLDD63 pKa = 3.27FVGYY67 pKa = 9.24PRR69 pKa = 11.84FPAPVEE75 pKa = 4.42FISAVIAYY83 pKa = 7.79YY84 pKa = 8.7VHH86 pKa = 6.96PVNIQTACLIMEE98 pKa = 4.57GAEE101 pKa = 3.88FTEE104 pKa = 4.67NIVNGVEE111 pKa = 4.44SPVKK115 pKa = 10.44ASEE118 pKa = 3.96LFAFVLRR125 pKa = 11.84VRR127 pKa = 11.84AGCKK131 pKa = 9.8DD132 pKa = 3.6VLEE135 pKa = 4.51GAEE138 pKa = 3.99DD139 pKa = 3.92NIRR142 pKa = 11.84AKK144 pKa = 10.71LRR146 pKa = 11.84AQGIMM151 pKa = 3.61

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T5F7|A0A4D6T5F7_9VIRU H protein OS=Escherichia phage Lilleen OX=2562233 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.38KK3 pKa = 10.54FNLALKK9 pKa = 9.73NSRR12 pKa = 11.84GSYY15 pKa = 9.45FATFRR20 pKa = 11.84HH21 pKa = 5.28QLAILSKK28 pKa = 10.81ADD30 pKa = 3.64ALDD33 pKa = 3.61EE34 pKa = 4.42EE35 pKa = 4.87KK36 pKa = 10.08WLNMMGALLKK46 pKa = 10.7DD47 pKa = 3.22WFRR50 pKa = 11.84YY51 pKa = 8.98EE52 pKa = 3.55EE53 pKa = 4.4HH54 pKa = 6.5FVHH57 pKa = 7.12GKK59 pKa = 9.55QSLVDD64 pKa = 3.34ILIEE68 pKa = 4.66RR69 pKa = 11.84GLLSPTQTTRR79 pKa = 11.84NNKK82 pKa = 9.63GNTKK86 pKa = 10.42

Molecular weight: 10.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1747

86

544

291.2

32.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.502 ± 1.087

0.859 ± 0.245

5.839 ± 0.469

3.721 ± 0.579

3.492 ± 0.584

6.525 ± 1.051

2.118 ± 0.698

5.438 ± 0.734

5.552 ± 0.976

8.357 ± 0.964

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.977 ± 0.415

5.152 ± 0.33

4.236 ± 0.494

4.98 ± 1.012

5.381 ± 0.721

7.27 ± 0.792

7.613 ± 0.806

5.781 ± 1.055

1.317 ± 0.304

3.892 ± 0.836
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski