Cellulomonas soli
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3847 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A512PAN6|A0A512PAN6_9CELL Uncharacterized protein OS=Cellulomonas soli OX=931535 GN=CSO01_09940 PE=4 SV=1
MM1 pKa = 7.52 CLAGATAWALDD12 pKa = 3.57 RR13 pKa = 11.84 FVIEE17 pKa = 4.27 HH18 pKa = 5.82 VEE20 pKa = 3.95 IADD23 pKa = 3.51 VSAYY27 pKa = 9.29 EE28 pKa = 4.0 AAQTGTVTTSTTTSASDD45 pKa = 3.63 ALVTDD50 pKa = 4.12 TTYY53 pKa = 11.27 EE54 pKa = 3.99 SDD56 pKa = 3.84 DD57 pKa = 3.73 ASITISTVTTGSGEE71 pKa = 4.1 ATVTYY76 pKa = 9.96 YY77 pKa = 11.02 VADD80 pKa = 3.68 VTLTDD85 pKa = 3.51 ATVLRR90 pKa = 11.84 SAFAQDD96 pKa = 3.45 AFGEE100 pKa = 4.45 NITEE104 pKa = 4.12 NTSDD108 pKa = 3.16 IAADD112 pKa = 3.53 NDD114 pKa = 3.76 AVFAINGDD122 pKa = 3.87 YY123 pKa = 11.14 YY124 pKa = 11.14 GFRR127 pKa = 11.84 STGIVIRR134 pKa = 11.84 NGVVYY139 pKa = 9.99 RR140 pKa = 11.84 DD141 pKa = 3.19 EE142 pKa = 4.38 GARR145 pKa = 11.84 EE146 pKa = 3.7 GLAFYY151 pKa = 10.56 RR152 pKa = 11.84 DD153 pKa = 3.68 GHH155 pKa = 6.34 VEE157 pKa = 4.21 VYY159 pKa = 11.0 DD160 pKa = 3.79 EE161 pKa = 4.39 TTTTAEE167 pKa = 4.03 EE168 pKa = 3.92 LLAAGVWNTLSFGPALLEE186 pKa = 4.74 DD187 pKa = 4.16 GQVVDD192 pKa = 5.36 GIEE195 pKa = 4.05 DD196 pKa = 3.81 VEE198 pKa = 4.28 VDD200 pKa = 3.87 TNVGNHH206 pKa = 6.74 SIQGDD211 pKa = 3.79 QPRR214 pKa = 11.84 TAIGVIDD221 pKa = 4.7 DD222 pKa = 3.62 NHH224 pKa = 6.99 LVFVVVDD231 pKa = 4.2 GRR233 pKa = 11.84 STGYY237 pKa = 10.15 SAGVDD242 pKa = 3.54 MTEE245 pKa = 4.72 LAQIMADD252 pKa = 3.94 LGATTAYY259 pKa = 10.52 NIDD262 pKa = 3.82 GGGSSTMVFDD272 pKa = 5.14 GEE274 pKa = 4.86 LVNNPLGKK282 pKa = 8.98 GTEE285 pKa = 4.16 RR286 pKa = 11.84 GTSDD290 pKa = 2.78 ILYY293 pKa = 10.14 IAGG296 pKa = 3.68
Molecular weight: 31.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.062
Thurlkill 3.541
EMBOSS 3.656
Sillero 3.834
Patrickios 1.189
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A512PD09|A0A512PD09_9CELL Uncharacterized protein OS=Cellulomonas soli OX=931535 GN=CSO01_18050 PE=4 SV=1
MM1 pKa = 6.74 VTPSHH6 pKa = 6.75 LRR8 pKa = 11.84 RR9 pKa = 11.84 TVAPAVVLGTRR20 pKa = 11.84 PPRR23 pKa = 11.84 VARR26 pKa = 11.84 RR27 pKa = 11.84 ARR29 pKa = 11.84 GGRR32 pKa = 11.84 ALARR36 pKa = 11.84 VVVLAAVVGVLVAVLLVAPASSGAGEE62 pKa = 4.28 LLVGAVAFGSTLLGAGAVVLVRR84 pKa = 11.84 RR85 pKa = 11.84 LRR87 pKa = 11.84 RR88 pKa = 11.84 SRR90 pKa = 11.84 TRR92 pKa = 3.06
Molecular weight: 9.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.472
IPC2_protein 11.038
IPC_protein 12.647
Toseland 12.808
ProMoST 13.32
Dawson 12.808
Bjellqvist 12.808
Wikipedia 13.29
Rodwell 12.31
Grimsley 12.852
Solomon 13.32
Lehninger 13.217
Nozaki 12.808
DTASelect 12.808
Thurlkill 12.808
EMBOSS 13.32
Sillero 12.808
Patrickios 12.047
IPC_peptide 13.32
IPC2_peptide 12.31
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3847
0
3847
1283081
39
2578
333.5
35.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.434 ± 0.053
0.568 ± 0.009
6.402 ± 0.033
5.09 ± 0.037
2.46 ± 0.022
9.379 ± 0.037
2.123 ± 0.023
2.875 ± 0.028
1.292 ± 0.023
10.495 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.533 ± 0.013
1.383 ± 0.018
5.932 ± 0.035
2.847 ± 0.019
7.642 ± 0.044
5.173 ± 0.036
6.751 ± 0.05
10.294 ± 0.043
1.536 ± 0.019
1.791 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here