Equid alphaherpesvirus 3
Average proteome isoelectric point is 6.79
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A077B9Q8|A0A077B9Q8_9ALPH Thymidine kinase OS=Equid alphaherpesvirus 3 OX=80341 GN=ORF38 PE=3 SV=1
MM1 pKa = 7.26 SSSVVSAPAEE11 pKa = 4.08 SPLAEE16 pKa = 4.13 EE17 pKa = 4.7 VSAVAQQICASEE29 pKa = 4.16 APAAGFHH36 pKa = 6.01 ATSGDD41 pKa = 3.24 HH42 pKa = 6.41 TYY44 pKa = 11.27 AGAASTPASANDD56 pKa = 4.41 CIEE59 pKa = 4.43 AMDD62 pKa = 4.07 TEE64 pKa = 4.53 EE65 pKa = 4.34 LLEE68 pKa = 4.16 MVLDD72 pKa = 4.02 AEE74 pKa = 5.07 GEE76 pKa = 4.54 GEE78 pKa = 4.55 SEE80 pKa = 4.48 APQPAQPAFTLKK92 pKa = 11.0 GNFICCSDD100 pKa = 3.93 EE101 pKa = 3.8 EE102 pKa = 4.39 CRR104 pKa = 11.84 ACLEE108 pKa = 4.18 LPFRR112 pKa = 11.84 PSEE115 pKa = 3.88 IGFSRR120 pKa = 11.84 DD121 pKa = 3.14 PHH123 pKa = 7.61 VSMALDD129 pKa = 3.79 MTCGTWIYY137 pKa = 10.05 IPRR140 pKa = 11.84 VFSDD144 pKa = 3.75 TPVCPWMANYY154 pKa = 9.22 CIPDD158 pKa = 3.67 LDD160 pKa = 4.71 EE161 pKa = 4.32 IADD164 pKa = 4.23 RR165 pKa = 11.84 EE166 pKa = 4.32
Molecular weight: 17.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.853
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.656
ProMoST 3.935
Dawson 3.795
Bjellqvist 3.999
Wikipedia 3.694
Rodwell 3.668
Grimsley 3.567
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.062
Thurlkill 3.694
EMBOSS 3.706
Sillero 3.948
Patrickios 0.299
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.886
Protein with the highest isoelectric point:
>tr|A0A077B7H6|A0A077B7H6_9ALPH DNA primase OS=Equid alphaherpesvirus 3 OX=80341 GN=ORF7 PE=3 SV=1
MM1 pKa = 7.51 ARR3 pKa = 11.84 RR4 pKa = 11.84 PGRR7 pKa = 11.84 CAARR11 pKa = 11.84 TSPPKK16 pKa = 9.83 PLPPEE21 pKa = 4.31 PSLSAGAVRR30 pKa = 11.84 RR31 pKa = 11.84 SGLKK35 pKa = 10.57 ASDD38 pKa = 4.0 LGASNARR45 pKa = 11.84 PRR47 pKa = 11.84 PQSQRR52 pKa = 11.84 PLQRR56 pKa = 11.84 LLAVPAIGGMAKK68 pKa = 10.29 FFRR71 pKa = 11.84 KK72 pKa = 9.51 KK73 pKa = 10.32 RR74 pKa = 11.84 GGARR78 pKa = 11.84 SRR80 pKa = 11.84 SVWYY84 pKa = 9.9 EE85 pKa = 3.54 GGTAFPPSAWKK96 pKa = 10.58 GPARR100 pKa = 11.84 TSPYY104 pKa = 10.31 GYY106 pKa = 10.36 EE107 pKa = 4.06 SDD109 pKa = 4.01 SDD111 pKa = 3.71 ADD113 pKa = 3.54 EE114 pKa = 4.47 ASRR117 pKa = 11.84 LRR119 pKa = 11.84 QGDD122 pKa = 3.64 GDD124 pKa = 3.88 RR125 pKa = 11.84 SRR127 pKa = 11.84 LASGAAGVRR136 pKa = 11.84 RR137 pKa = 11.84 EE138 pKa = 4.29 LGVEE142 pKa = 3.84 AGSPAVLPLNGSRR155 pKa = 11.84 RR156 pKa = 11.84 RR157 pKa = 11.84 DD158 pKa = 3.47 PTPADD163 pKa = 3.47 VYY165 pKa = 11.49 LDD167 pKa = 3.69 GFPTLRR173 pKa = 11.84 EE174 pKa = 3.98 QEE176 pKa = 4.26 EE177 pKa = 4.4 LQLAPRR183 pKa = 11.84 PVQKK187 pKa = 10.68 SGGAGACCQRR197 pKa = 11.84 LVIANVVILLFQVLVLTGTIFYY219 pKa = 10.03 SLHH222 pKa = 6.56 HH223 pKa = 5.75 GHH225 pKa = 7.1 CNGKK229 pKa = 8.39 QQ230 pKa = 2.9
Molecular weight: 24.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.692
IPC_protein 10.745
Toseland 10.818
ProMoST 10.73
Dawson 10.891
Bjellqvist 10.687
Wikipedia 11.184
Rodwell 10.95
Grimsley 10.95
Solomon 11.111
Lehninger 11.052
Nozaki 10.818
DTASelect 10.687
Thurlkill 10.818
EMBOSS 11.242
Sillero 10.847
Patrickios 10.657
IPC_peptide 11.111
IPC2_peptide 9.955
IPC2.peptide.svr19 8.648
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
76
1
77
39868
74
3353
517.8
56.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.352 ± 0.583
1.881 ± 0.148
5.127 ± 0.128
5.799 ± 0.179
3.602 ± 0.182
7.698 ± 0.192
2.122 ± 0.102
2.784 ± 0.141
2.335 ± 0.166
9.624 ± 0.18
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.808 ± 0.12
2.541 ± 0.167
7.675 ± 0.39
2.594 ± 0.132
8.498 ± 0.197
6.75 ± 0.167
4.991 ± 0.212
6.988 ± 0.219
1.031 ± 0.065
2.799 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here