Equid alphaherpesvirus 3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A077B9Q8|A0A077B9Q8_9ALPH Thymidine kinase OS=Equid alphaherpesvirus 3 OX=80341 GN=ORF38 PE=3 SV=1
MM1 pKa = 7.26SSSVVSAPAEE11 pKa = 4.08SPLAEE16 pKa = 4.13EE17 pKa = 4.7VSAVAQQICASEE29 pKa = 4.16APAAGFHH36 pKa = 6.01ATSGDD41 pKa = 3.24HH42 pKa = 6.41TYY44 pKa = 11.27AGAASTPASANDD56 pKa = 4.41CIEE59 pKa = 4.43AMDD62 pKa = 4.07TEE64 pKa = 4.53EE65 pKa = 4.34LLEE68 pKa = 4.16MVLDD72 pKa = 4.02AEE74 pKa = 5.07GEE76 pKa = 4.54GEE78 pKa = 4.55SEE80 pKa = 4.48APQPAQPAFTLKK92 pKa = 11.0GNFICCSDD100 pKa = 3.93EE101 pKa = 3.8EE102 pKa = 4.39CRR104 pKa = 11.84ACLEE108 pKa = 4.18LPFRR112 pKa = 11.84PSEE115 pKa = 3.88IGFSRR120 pKa = 11.84DD121 pKa = 3.14PHH123 pKa = 7.61VSMALDD129 pKa = 3.79MTCGTWIYY137 pKa = 10.05IPRR140 pKa = 11.84VFSDD144 pKa = 3.75TPVCPWMANYY154 pKa = 9.22CIPDD158 pKa = 3.67LDD160 pKa = 4.71EE161 pKa = 4.32IADD164 pKa = 4.23RR165 pKa = 11.84EE166 pKa = 4.32

Molecular weight:
17.68 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A077B7H6|A0A077B7H6_9ALPH DNA primase OS=Equid alphaherpesvirus 3 OX=80341 GN=ORF7 PE=3 SV=1
MM1 pKa = 7.51ARR3 pKa = 11.84RR4 pKa = 11.84PGRR7 pKa = 11.84CAARR11 pKa = 11.84TSPPKK16 pKa = 9.83PLPPEE21 pKa = 4.31PSLSAGAVRR30 pKa = 11.84RR31 pKa = 11.84SGLKK35 pKa = 10.57ASDD38 pKa = 4.0LGASNARR45 pKa = 11.84PRR47 pKa = 11.84PQSQRR52 pKa = 11.84PLQRR56 pKa = 11.84LLAVPAIGGMAKK68 pKa = 10.29FFRR71 pKa = 11.84KK72 pKa = 9.51KK73 pKa = 10.32RR74 pKa = 11.84GGARR78 pKa = 11.84SRR80 pKa = 11.84SVWYY84 pKa = 9.9EE85 pKa = 3.54GGTAFPPSAWKK96 pKa = 10.58GPARR100 pKa = 11.84TSPYY104 pKa = 10.31GYY106 pKa = 10.36EE107 pKa = 4.06SDD109 pKa = 4.01SDD111 pKa = 3.71ADD113 pKa = 3.54EE114 pKa = 4.47ASRR117 pKa = 11.84LRR119 pKa = 11.84QGDD122 pKa = 3.64GDD124 pKa = 3.88RR125 pKa = 11.84SRR127 pKa = 11.84LASGAAGVRR136 pKa = 11.84RR137 pKa = 11.84EE138 pKa = 4.29LGVEE142 pKa = 3.84AGSPAVLPLNGSRR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84DD158 pKa = 3.47PTPADD163 pKa = 3.47VYY165 pKa = 11.49LDD167 pKa = 3.69GFPTLRR173 pKa = 11.84EE174 pKa = 3.98QEE176 pKa = 4.26EE177 pKa = 4.4LQLAPRR183 pKa = 11.84PVQKK187 pKa = 10.68SGGAGACCQRR197 pKa = 11.84LVIANVVILLFQVLVLTGTIFYY219 pKa = 10.03SLHH222 pKa = 6.56HH223 pKa = 5.75GHH225 pKa = 7.1CNGKK229 pKa = 8.39QQ230 pKa = 2.9

Molecular weight:
24.57 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

1

77

39868

74

3353

517.8

56.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.352 ± 0.583

1.881 ± 0.148

5.127 ± 0.128

5.799 ± 0.179

3.602 ± 0.182

7.698 ± 0.192

2.122 ± 0.102

2.784 ± 0.141

2.335 ± 0.166

9.624 ± 0.18

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.808 ± 0.12

2.541 ± 0.167

7.675 ± 0.39

2.594 ± 0.132

8.498 ± 0.197

6.75 ± 0.167

4.991 ± 0.212

6.988 ± 0.219

1.031 ± 0.065

2.799 ± 0.162

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski