Hubei tetragnatha maxillosa virus 8
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLM1|A0A1L3KLM1_9VIRU Uncharacterized protein OS=Hubei tetragnatha maxillosa virus 8 OX=1923250 PE=4 SV=1
MM1 pKa = 8.07 LEE3 pKa = 4.32 DD4 pKa = 3.98 KK5 pKa = 10.58 DD6 pKa = 4.14 YY7 pKa = 11.53 EE8 pKa = 4.93 SVYY11 pKa = 11.28 DD12 pKa = 3.68 WLNDD16 pKa = 3.35 YY17 pKa = 7.67 TTSIEE22 pKa = 4.26 KK23 pKa = 9.44 MDD25 pKa = 3.75 AVEE28 pKa = 4.47 RR29 pKa = 11.84 RR30 pKa = 11.84 QEE32 pKa = 3.72 TRR34 pKa = 11.84 EE35 pKa = 3.99 DD36 pKa = 3.59 YY37 pKa = 10.96 RR38 pKa = 11.84 EE39 pKa = 3.88 EE40 pKa = 4.12 SQIKK44 pKa = 10.13 ADD46 pKa = 3.59 YY47 pKa = 10.58 LDD49 pKa = 5.74 YY50 pKa = 10.77 VDD52 pKa = 3.08 EE53 pKa = 4.72 WKK55 pKa = 9.12 WHH57 pKa = 5.18 NKK59 pKa = 6.27 EE60 pKa = 4.03 QKK62 pKa = 9.89 HH63 pKa = 4.69 QLLEE67 pKa = 4.08 AFLYY71 pKa = 10.79 SISQTHH77 pKa = 5.79 QIVRR81 pKa = 11.84 CNCGNTHH88 pKa = 6.75 TFTTMAQRR96 pKa = 11.84 EE97 pKa = 4.21 EE98 pKa = 4.38 GEE100 pKa = 4.11 IAIDD104 pKa = 3.81 EE105 pKa = 4.15 PTILIFTQCWYY116 pKa = 10.34 IHH118 pKa = 6.86 ISNQISIALDD128 pKa = 3.38 SLTEE132 pKa = 3.9 EE133 pKa = 5.02 QIRR136 pKa = 11.84 QPTYY140 pKa = 9.63 VEE142 pKa = 4.7 DD143 pKa = 3.58 VLGYY147 pKa = 7.51 VAKK150 pKa = 10.31 WKK152 pKa = 8.4 VTHH155 pKa = 6.64 NLTPMLPLDD164 pKa = 3.85 VFKK167 pKa = 10.96 KK168 pKa = 9.83 IWWDD172 pKa = 3.1 KK173 pKa = 7.14 TLKK176 pKa = 10.5 NKK178 pKa = 10.32 RR179 pKa = 11.84 STDD182 pKa = 3.9 AIEE185 pKa = 4.63 LEE187 pKa = 4.25 QKK189 pKa = 10.26 IPSNDD194 pKa = 2.84 RR195 pKa = 11.84 TIIHH199 pKa = 7.2 AKK201 pKa = 8.51 QKK203 pKa = 10.69 EE204 pKa = 3.91 YY205 pKa = 10.76 DD206 pKa = 3.21 APIRR210 pKa = 11.84 HH211 pKa = 6.25 EE212 pKa = 3.94 GHH214 pKa = 6.37 RR215 pKa = 11.84 VLLHH219 pKa = 5.97 FVHH222 pKa = 6.67 NEE224 pKa = 3.66 HH225 pKa = 6.83 SFHH228 pKa = 6.4 NLYY231 pKa = 10.36 HH232 pKa = 6.8 ALSKK236 pKa = 11.11 ADD238 pKa = 3.89 FLIVLKK244 pKa = 10.5 FMNTWRR250 pKa = 11.84 TKK252 pKa = 10.06 IGRR255 pKa = 11.84 SSDD258 pKa = 3.88 KK259 pKa = 10.52 IDD261 pKa = 3.49 LTDD264 pKa = 3.63 IEE266 pKa = 4.51 ASVMKK271 pKa = 10.19 CEE273 pKa = 3.76 RR274 pKa = 11.84 CMEE277 pKa = 3.84 IKK279 pKa = 9.87 TRR281 pKa = 11.84 VKK283 pKa = 9.47 LTLPGVYY290 pKa = 9.36 YY291 pKa = 10.76 APPGVGKK298 pKa = 6.95 TTAMEE303 pKa = 4.27 SGQIVGVDD311 pKa = 3.57 TDD313 pKa = 4.38 WIGVGPTWRR322 pKa = 11.84 DD323 pKa = 3.0 YY324 pKa = 11.01 STIFKK329 pKa = 9.7 MKK331 pKa = 10.2 IPIVTNQYY339 pKa = 8.67 TAFVGCGYY347 pKa = 10.79 KK348 pKa = 9.0 VTGVVKK354 pKa = 10.58 SSIRR358 pKa = 11.84 KK359 pKa = 9.4 DD360 pKa = 3.54 SEE362 pKa = 4.02 GKK364 pKa = 9.83 PFTTKK369 pKa = 10.18 QQLLKK374 pKa = 9.97 YY375 pKa = 9.27 AKK377 pKa = 8.65 SHH379 pKa = 6.05 PVDD382 pKa = 3.04 VHH384 pKa = 6.54 FIVSSEE390 pKa = 3.86 KK391 pKa = 10.75 KK392 pKa = 9.87 FFQDD396 pKa = 3.0 HH397 pKa = 5.1 VLRR400 pKa = 11.84 LQIIQQMHH408 pKa = 5.75 YY409 pKa = 10.63 LIANYY414 pKa = 10.71 SMNQMPFYY422 pKa = 9.88 RR423 pKa = 11.84 TEE425 pKa = 4.05 QSEE428 pKa = 4.63 EE429 pKa = 3.75 WAKK432 pKa = 10.2 TYY434 pKa = 10.03 PKK436 pKa = 10.58 LLRR439 pKa = 11.84 KK440 pKa = 9.52 EE441 pKa = 4.21 KK442 pKa = 10.41 FIKK445 pKa = 10.33 LYY447 pKa = 10.56 NINRR451 pKa = 3.84
Molecular weight: 53.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.759
IPC2_protein 6.971
IPC_protein 7.029
Toseland 7.073
ProMoST 7.629
Dawson 7.644
Bjellqvist 7.673
Wikipedia 7.585
Rodwell 7.644
Grimsley 7.205
Solomon 7.702
Lehninger 7.717
Nozaki 7.907
DTASelect 7.819
Thurlkill 7.863
EMBOSS 7.922
Sillero 8.039
Patrickios 4.266
IPC_peptide 7.702
IPC2_peptide 7.161
IPC2.peptide.svr19 7.18
Protein with the highest isoelectric point:
>tr|A0A1L3KLM1|A0A1L3KLM1_9VIRU Uncharacterized protein OS=Hubei tetragnatha maxillosa virus 8 OX=1923250 PE=4 SV=1
MM1 pKa = 7.83 DD2 pKa = 3.81 VANEE6 pKa = 4.01 RR7 pKa = 11.84 SKK9 pKa = 11.32 VLQDD13 pKa = 3.14 TRR15 pKa = 11.84 FFKK18 pKa = 10.48 PLPRR22 pKa = 11.84 GTGFPFNYY30 pKa = 9.54 QPSRR34 pKa = 11.84 LDD36 pKa = 3.42 RR37 pKa = 11.84 NALFALNRR45 pKa = 11.84 VYY47 pKa = 10.76 DD48 pKa = 3.85 PEE50 pKa = 6.34 KK51 pKa = 9.99 ISEE54 pKa = 4.11 VRR56 pKa = 11.84 TKK58 pKa = 8.59 YY59 pKa = 10.33 HH60 pKa = 6.05 RR61 pKa = 11.84 AKK63 pKa = 11.07 LNMEE67 pKa = 4.73 DD68 pKa = 4.78 LRR70 pKa = 11.84 TDD72 pKa = 3.56 LLEE75 pKa = 4.03 YY76 pKa = 10.72 SCAKK80 pKa = 9.74 VPRR83 pKa = 11.84 IQDD86 pKa = 3.08 DD87 pKa = 4.08 SYY89 pKa = 11.47 YY90 pKa = 10.18 MIFEE94 pKa = 4.42 SVDD97 pKa = 2.95 KK98 pKa = 11.17 DD99 pKa = 3.06 IFGDD103 pKa = 3.55 MKK105 pKa = 10.91 VIPLTHH111 pKa = 6.21 GAVANHH117 pKa = 7.33 PDD119 pKa = 3.7 LPRR122 pKa = 11.84 QKK124 pKa = 10.88 SPGLPYY130 pKa = 9.2 KK131 pKa = 8.69 TQGYY135 pKa = 5.97 ATKK138 pKa = 10.87 GEE140 pKa = 4.26 ALDD143 pKa = 5.59 DD144 pKa = 4.77 PNTLKK149 pKa = 10.88 NIRR152 pKa = 11.84 ATWYY156 pKa = 10.13 AIEE159 pKa = 4.33 RR160 pKa = 11.84 SEE162 pKa = 5.65 DD163 pKa = 3.24 VTLPDD168 pKa = 3.28 VACYY172 pKa = 10.43 ARR174 pKa = 11.84 AQICSRR180 pKa = 11.84 DD181 pKa = 3.59 KK182 pKa = 11.4 NKK184 pKa = 9.4 IRR186 pKa = 11.84 ATWGYY191 pKa = 8.46 PLSVFLTEE199 pKa = 4.0 AQYY202 pKa = 10.32 FYY204 pKa = 10.73 PILKK208 pKa = 9.61 EE209 pKa = 3.72 LKK211 pKa = 10.08 QRR213 pKa = 11.84 DD214 pKa = 4.1 KK215 pKa = 11.4 PKK217 pKa = 9.68 IAYY220 pKa = 9.2 GVEE223 pKa = 3.63 IGTGGMGFLNEE234 pKa = 3.79 MASYY238 pKa = 11.05 YY239 pKa = 8.98 KK240 pKa = 10.45 TNNFLIGDD248 pKa = 3.49 WRR250 pKa = 11.84 KK251 pKa = 9.23 FDD253 pKa = 3.46 KK254 pKa = 10.77 TIPAWLIRR262 pKa = 11.84 DD263 pKa = 4.32 AFRR266 pKa = 11.84 MIMRR270 pKa = 11.84 HH271 pKa = 5.57 IDD273 pKa = 3.41 LTKK276 pKa = 9.65 VQSTGGRR283 pKa = 11.84 IWPVRR288 pKa = 11.84 ASKK291 pKa = 9.33 TKK293 pKa = 10.22 KK294 pKa = 10.21 RR295 pKa = 11.84 LAKK298 pKa = 10.14 LINYY302 pKa = 8.54 FIDD305 pKa = 3.64 TPVQLSSGEE314 pKa = 4.01 RR315 pKa = 11.84 FVKK318 pKa = 10.54 HH319 pKa = 5.98 GGVPSGSSFTNLIDD333 pKa = 4.73 GIVNAIVTRR342 pKa = 11.84 YY343 pKa = 6.9 TVYY346 pKa = 11.3 SMTGEE351 pKa = 4.11 LPYY354 pKa = 11.16 DD355 pKa = 3.83 DD356 pKa = 6.19 LYY358 pKa = 11.71 LGDD361 pKa = 5.87 DD362 pKa = 3.29 IVAVTKK368 pKa = 10.76 KK369 pKa = 10.38 PLDD372 pKa = 3.34 LHH374 pKa = 6.62 LFAEE378 pKa = 4.59 IAEE381 pKa = 4.68 KK382 pKa = 9.9 QFSMIFNPDD391 pKa = 2.56 KK392 pKa = 11.0 SYY394 pKa = 9.32 QTSQKK399 pKa = 10.8 EE400 pKa = 4.19 NIHH403 pKa = 5.88 FLGYY407 pKa = 10.43 YY408 pKa = 9.1 NIHH411 pKa = 5.77 GMPYY415 pKa = 9.91 KK416 pKa = 10.56 PVDD419 pKa = 3.54 TVIASSIYY427 pKa = 9.62 PEE429 pKa = 4.72 RR430 pKa = 11.84 PCEE433 pKa = 4.15 TKK435 pKa = 10.64 FEE437 pKa = 4.85 TITRR441 pKa = 11.84 LIGQAYY447 pKa = 10.04 SCFEE451 pKa = 4.15 PEE453 pKa = 3.59 DD454 pKa = 3.37 AKK456 pKa = 11.17 RR457 pKa = 11.84 FFLAAQMLQDD467 pKa = 4.24 EE468 pKa = 5.16 VPGLTNQMITEE479 pKa = 4.58 FTKK482 pKa = 11.02 DD483 pKa = 2.82 HH484 pKa = 5.31 THH486 pKa = 5.57 WFKK489 pKa = 11.1 YY490 pKa = 9.14 LQTVGVSTRR499 pKa = 11.84 DD500 pKa = 3.4 GLTMPKK506 pKa = 9.85 VRR508 pKa = 11.84 TYY510 pKa = 10.88 DD511 pKa = 3.64 QIWITAPQAPRR522 pKa = 11.84 RR523 pKa = 11.84 KK524 pKa = 8.37 WSPVVHH530 pKa = 7.21 DD531 pKa = 5.15 LEE533 pKa = 4.56 QLAQLAYY540 pKa = 9.51 EE541 pKa = 3.96 KK542 pKa = 10.4 WKK544 pKa = 11.08 VEE546 pKa = 3.97 EE547 pKa = 4.41 EE548 pKa = 4.26 DD549 pKa = 5.38 NIDD552 pKa = 3.58 STT554 pKa = 4.89
Molecular weight: 63.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.498
IPC2_protein 7.849
IPC_protein 7.761
Toseland 7.863
ProMoST 8.229
Dawson 8.521
Bjellqvist 8.595
Wikipedia 8.565
Rodwell 8.58
Grimsley 7.922
Solomon 8.624
Lehninger 8.609
Nozaki 8.624
DTASelect 8.463
Thurlkill 8.521
EMBOSS 8.697
Sillero 8.741
Patrickios 4.329
IPC_peptide 8.624
IPC2_peptide 7.351
IPC2.peptide.svr19 7.66
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1904
438
554
476.0
54.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.935 ± 0.616
1.103 ± 0.167
6.197 ± 0.488
6.513 ± 0.417
3.624 ± 0.421
4.674 ± 0.479
2.363 ± 0.513
7.458 ± 0.49
6.985 ± 0.755
7.09 ± 0.296
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.311 ± 0.208
4.832 ± 0.647
4.884 ± 0.829
5.147 ± 0.518
6.197 ± 0.468
5.725 ± 0.767
7.038 ± 0.167
6.25 ± 0.28
1.576 ± 0.257
4.097 ± 0.618
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here