Hubei tetragnatha maxillosa virus 8

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KLM1|A0A1L3KLM1_9VIRU Uncharacterized protein OS=Hubei tetragnatha maxillosa virus 8 OX=1923250 PE=4 SV=1
MM1 pKa = 8.07LEE3 pKa = 4.32DD4 pKa = 3.98KK5 pKa = 10.58DD6 pKa = 4.14YY7 pKa = 11.53EE8 pKa = 4.93SVYY11 pKa = 11.28DD12 pKa = 3.68WLNDD16 pKa = 3.35YY17 pKa = 7.67TTSIEE22 pKa = 4.26KK23 pKa = 9.44MDD25 pKa = 3.75AVEE28 pKa = 4.47RR29 pKa = 11.84RR30 pKa = 11.84QEE32 pKa = 3.72TRR34 pKa = 11.84EE35 pKa = 3.99DD36 pKa = 3.59YY37 pKa = 10.96RR38 pKa = 11.84EE39 pKa = 3.88EE40 pKa = 4.12SQIKK44 pKa = 10.13ADD46 pKa = 3.59YY47 pKa = 10.58LDD49 pKa = 5.74YY50 pKa = 10.77VDD52 pKa = 3.08EE53 pKa = 4.72WKK55 pKa = 9.12WHH57 pKa = 5.18NKK59 pKa = 6.27EE60 pKa = 4.03QKK62 pKa = 9.89HH63 pKa = 4.69QLLEE67 pKa = 4.08AFLYY71 pKa = 10.79SISQTHH77 pKa = 5.79QIVRR81 pKa = 11.84CNCGNTHH88 pKa = 6.75TFTTMAQRR96 pKa = 11.84EE97 pKa = 4.21EE98 pKa = 4.38GEE100 pKa = 4.11IAIDD104 pKa = 3.81EE105 pKa = 4.15PTILIFTQCWYY116 pKa = 10.34IHH118 pKa = 6.86ISNQISIALDD128 pKa = 3.38SLTEE132 pKa = 3.9EE133 pKa = 5.02QIRR136 pKa = 11.84QPTYY140 pKa = 9.63VEE142 pKa = 4.7DD143 pKa = 3.58VLGYY147 pKa = 7.51VAKK150 pKa = 10.31WKK152 pKa = 8.4VTHH155 pKa = 6.64NLTPMLPLDD164 pKa = 3.85VFKK167 pKa = 10.96KK168 pKa = 9.83IWWDD172 pKa = 3.1KK173 pKa = 7.14TLKK176 pKa = 10.5NKK178 pKa = 10.32RR179 pKa = 11.84STDD182 pKa = 3.9AIEE185 pKa = 4.63LEE187 pKa = 4.25QKK189 pKa = 10.26IPSNDD194 pKa = 2.84RR195 pKa = 11.84TIIHH199 pKa = 7.2AKK201 pKa = 8.51QKK203 pKa = 10.69EE204 pKa = 3.91YY205 pKa = 10.76DD206 pKa = 3.21APIRR210 pKa = 11.84HH211 pKa = 6.25EE212 pKa = 3.94GHH214 pKa = 6.37RR215 pKa = 11.84VLLHH219 pKa = 5.97FVHH222 pKa = 6.67NEE224 pKa = 3.66HH225 pKa = 6.83SFHH228 pKa = 6.4NLYY231 pKa = 10.36HH232 pKa = 6.8ALSKK236 pKa = 11.11ADD238 pKa = 3.89FLIVLKK244 pKa = 10.5FMNTWRR250 pKa = 11.84TKK252 pKa = 10.06IGRR255 pKa = 11.84SSDD258 pKa = 3.88KK259 pKa = 10.52IDD261 pKa = 3.49LTDD264 pKa = 3.63IEE266 pKa = 4.51ASVMKK271 pKa = 10.19CEE273 pKa = 3.76RR274 pKa = 11.84CMEE277 pKa = 3.84IKK279 pKa = 9.87TRR281 pKa = 11.84VKK283 pKa = 9.47LTLPGVYY290 pKa = 9.36YY291 pKa = 10.76APPGVGKK298 pKa = 6.95TTAMEE303 pKa = 4.27SGQIVGVDD311 pKa = 3.57TDD313 pKa = 4.38WIGVGPTWRR322 pKa = 11.84DD323 pKa = 3.0YY324 pKa = 11.01STIFKK329 pKa = 9.7MKK331 pKa = 10.2IPIVTNQYY339 pKa = 8.67TAFVGCGYY347 pKa = 10.79KK348 pKa = 9.0VTGVVKK354 pKa = 10.58SSIRR358 pKa = 11.84KK359 pKa = 9.4DD360 pKa = 3.54SEE362 pKa = 4.02GKK364 pKa = 9.83PFTTKK369 pKa = 10.18QQLLKK374 pKa = 9.97YY375 pKa = 9.27AKK377 pKa = 8.65SHH379 pKa = 6.05PVDD382 pKa = 3.04VHH384 pKa = 6.54FIVSSEE390 pKa = 3.86KK391 pKa = 10.75KK392 pKa = 9.87FFQDD396 pKa = 3.0HH397 pKa = 5.1VLRR400 pKa = 11.84LQIIQQMHH408 pKa = 5.75YY409 pKa = 10.63LIANYY414 pKa = 10.71SMNQMPFYY422 pKa = 9.88RR423 pKa = 11.84TEE425 pKa = 4.05QSEE428 pKa = 4.63EE429 pKa = 3.75WAKK432 pKa = 10.2TYY434 pKa = 10.03PKK436 pKa = 10.58LLRR439 pKa = 11.84KK440 pKa = 9.52EE441 pKa = 4.21KK442 pKa = 10.41FIKK445 pKa = 10.33LYY447 pKa = 10.56NINRR451 pKa = 3.84

Molecular weight:
53.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KLM1|A0A1L3KLM1_9VIRU Uncharacterized protein OS=Hubei tetragnatha maxillosa virus 8 OX=1923250 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 3.81VANEE6 pKa = 4.01RR7 pKa = 11.84SKK9 pKa = 11.32VLQDD13 pKa = 3.14TRR15 pKa = 11.84FFKK18 pKa = 10.48PLPRR22 pKa = 11.84GTGFPFNYY30 pKa = 9.54QPSRR34 pKa = 11.84LDD36 pKa = 3.42RR37 pKa = 11.84NALFALNRR45 pKa = 11.84VYY47 pKa = 10.76DD48 pKa = 3.85PEE50 pKa = 6.34KK51 pKa = 9.99ISEE54 pKa = 4.11VRR56 pKa = 11.84TKK58 pKa = 8.59YY59 pKa = 10.33HH60 pKa = 6.05RR61 pKa = 11.84AKK63 pKa = 11.07LNMEE67 pKa = 4.73DD68 pKa = 4.78LRR70 pKa = 11.84TDD72 pKa = 3.56LLEE75 pKa = 4.03YY76 pKa = 10.72SCAKK80 pKa = 9.74VPRR83 pKa = 11.84IQDD86 pKa = 3.08DD87 pKa = 4.08SYY89 pKa = 11.47YY90 pKa = 10.18MIFEE94 pKa = 4.42SVDD97 pKa = 2.95KK98 pKa = 11.17DD99 pKa = 3.06IFGDD103 pKa = 3.55MKK105 pKa = 10.91VIPLTHH111 pKa = 6.21GAVANHH117 pKa = 7.33PDD119 pKa = 3.7LPRR122 pKa = 11.84QKK124 pKa = 10.88SPGLPYY130 pKa = 9.2KK131 pKa = 8.69TQGYY135 pKa = 5.97ATKK138 pKa = 10.87GEE140 pKa = 4.26ALDD143 pKa = 5.59DD144 pKa = 4.77PNTLKK149 pKa = 10.88NIRR152 pKa = 11.84ATWYY156 pKa = 10.13AIEE159 pKa = 4.33RR160 pKa = 11.84SEE162 pKa = 5.65DD163 pKa = 3.24VTLPDD168 pKa = 3.28VACYY172 pKa = 10.43ARR174 pKa = 11.84AQICSRR180 pKa = 11.84DD181 pKa = 3.59KK182 pKa = 11.4NKK184 pKa = 9.4IRR186 pKa = 11.84ATWGYY191 pKa = 8.46PLSVFLTEE199 pKa = 4.0AQYY202 pKa = 10.32FYY204 pKa = 10.73PILKK208 pKa = 9.61EE209 pKa = 3.72LKK211 pKa = 10.08QRR213 pKa = 11.84DD214 pKa = 4.1KK215 pKa = 11.4PKK217 pKa = 9.68IAYY220 pKa = 9.2GVEE223 pKa = 3.63IGTGGMGFLNEE234 pKa = 3.79MASYY238 pKa = 11.05YY239 pKa = 8.98KK240 pKa = 10.45TNNFLIGDD248 pKa = 3.49WRR250 pKa = 11.84KK251 pKa = 9.23FDD253 pKa = 3.46KK254 pKa = 10.77TIPAWLIRR262 pKa = 11.84DD263 pKa = 4.32AFRR266 pKa = 11.84MIMRR270 pKa = 11.84HH271 pKa = 5.57IDD273 pKa = 3.41LTKK276 pKa = 9.65VQSTGGRR283 pKa = 11.84IWPVRR288 pKa = 11.84ASKK291 pKa = 9.33TKK293 pKa = 10.22KK294 pKa = 10.21RR295 pKa = 11.84LAKK298 pKa = 10.14LINYY302 pKa = 8.54FIDD305 pKa = 3.64TPVQLSSGEE314 pKa = 4.01RR315 pKa = 11.84FVKK318 pKa = 10.54HH319 pKa = 5.98GGVPSGSSFTNLIDD333 pKa = 4.73GIVNAIVTRR342 pKa = 11.84YY343 pKa = 6.9TVYY346 pKa = 11.3SMTGEE351 pKa = 4.11LPYY354 pKa = 11.16DD355 pKa = 3.83DD356 pKa = 6.19LYY358 pKa = 11.71LGDD361 pKa = 5.87DD362 pKa = 3.29IVAVTKK368 pKa = 10.76KK369 pKa = 10.38PLDD372 pKa = 3.34LHH374 pKa = 6.62LFAEE378 pKa = 4.59IAEE381 pKa = 4.68KK382 pKa = 9.9QFSMIFNPDD391 pKa = 2.56KK392 pKa = 11.0SYY394 pKa = 9.32QTSQKK399 pKa = 10.8EE400 pKa = 4.19NIHH403 pKa = 5.88FLGYY407 pKa = 10.43YY408 pKa = 9.1NIHH411 pKa = 5.77GMPYY415 pKa = 9.91KK416 pKa = 10.56PVDD419 pKa = 3.54TVIASSIYY427 pKa = 9.62PEE429 pKa = 4.72RR430 pKa = 11.84PCEE433 pKa = 4.15TKK435 pKa = 10.64FEE437 pKa = 4.85TITRR441 pKa = 11.84LIGQAYY447 pKa = 10.04SCFEE451 pKa = 4.15PEE453 pKa = 3.59DD454 pKa = 3.37AKK456 pKa = 11.17RR457 pKa = 11.84FFLAAQMLQDD467 pKa = 4.24EE468 pKa = 5.16VPGLTNQMITEE479 pKa = 4.58FTKK482 pKa = 11.02DD483 pKa = 2.82HH484 pKa = 5.31THH486 pKa = 5.57WFKK489 pKa = 11.1YY490 pKa = 9.14LQTVGVSTRR499 pKa = 11.84DD500 pKa = 3.4GLTMPKK506 pKa = 9.85VRR508 pKa = 11.84TYY510 pKa = 10.88DD511 pKa = 3.64QIWITAPQAPRR522 pKa = 11.84RR523 pKa = 11.84KK524 pKa = 8.37WSPVVHH530 pKa = 7.21DD531 pKa = 5.15LEE533 pKa = 4.56QLAQLAYY540 pKa = 9.51EE541 pKa = 3.96KK542 pKa = 10.4WKK544 pKa = 11.08VEE546 pKa = 3.97EE547 pKa = 4.41EE548 pKa = 4.26DD549 pKa = 5.38NIDD552 pKa = 3.58STT554 pKa = 4.89

Molecular weight:
63.89 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1904

438

554

476.0

54.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.935 ± 0.616

1.103 ± 0.167

6.197 ± 0.488

6.513 ± 0.417

3.624 ± 0.421

4.674 ± 0.479

2.363 ± 0.513

7.458 ± 0.49

6.985 ± 0.755

7.09 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.208

4.832 ± 0.647

4.884 ± 0.829

5.147 ± 0.518

6.197 ± 0.468

5.725 ± 0.767

7.038 ± 0.167

6.25 ± 0.28

1.576 ± 0.257

4.097 ± 0.618

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski