Hawaiian green turtle herpesvirus
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5ZR62|Q5ZR62_9ALPH Basic phosphorylated capsid protein OS=Hawaiian green turtle herpesvirus OX=70564 GN=UL35 PE=4 SV=1
TT1 pKa = 7.07 AAGPVSEE8 pKa = 4.6 TDD10 pKa = 3.29 PVLRR14 pKa = 11.84 SPLDD18 pKa = 3.52 RR19 pKa = 11.84 SEE21 pKa = 4.82 LNARR25 pKa = 11.84 DD26 pKa = 3.94 ALLLLAAFCPWHH38 pKa = 5.71 VALFARR44 pKa = 11.84 LPHH47 pKa = 6.01 VEE49 pKa = 3.84 QLEE52 pKa = 4.66 FVSKK56 pKa = 9.49 TLEE59 pKa = 4.23 PLLSEE64 pKa = 4.2 AAVGRR69 pKa = 11.84 LAVSRR74 pKa = 11.84 LSGKK78 pKa = 9.74 QGPRR82 pKa = 11.84 LLPVLSADD90 pKa = 3.72 EE91 pKa = 4.39 EE92 pKa = 4.23 KK93 pKa = 10.82 SPRR96 pKa = 11.84 VFGLRR101 pKa = 11.84 LRR103 pKa = 11.84 DD104 pKa = 3.19 WRR106 pKa = 11.84 SFQVTPSLTFPEE118 pKa = 4.07 IWLQTGAAEE127 pKa = 4.2 ATPSAEE133 pKa = 4.3 GLRR136 pKa = 11.84 EE137 pKa = 4.05 LQRR140 pKa = 11.84 LTDD143 pKa = 4.15 DD144 pKa = 3.91 GKK146 pKa = 11.16 AFATLLYY153 pKa = 10.35 ALAYY157 pKa = 9.77 HH158 pKa = 6.59 CVPPAILEE166 pKa = 4.27 MLWNALRR173 pKa = 11.84 PRR175 pKa = 11.84 DD176 pKa = 4.21 LPDD179 pKa = 3.55 EE180 pKa = 4.68 ADD182 pKa = 2.95 ASIFYY187 pKa = 10.14 RR188 pKa = 11.84 QRR190 pKa = 11.84 ALPGEE195 pKa = 4.24 SFVVSEE201 pKa = 4.52 STVALQTEE209 pKa = 4.51 RR210 pKa = 11.84 GEE212 pKa = 4.12 IPPDD216 pKa = 3.1 EE217 pKa = 4.66 RR218 pKa = 11.84 LWIPQARR225 pKa = 11.84 ARR227 pKa = 11.84 TLTVGEE233 pKa = 4.5 TRR235 pKa = 11.84 SKK237 pKa = 11.12 APPCAFDD244 pKa = 3.38 FAIFAVLTGVPLVIAATLAPGAGFSRR270 pKa = 11.84 EE271 pKa = 3.88 SGLFLGRR278 pKa = 11.84 VLLDD282 pKa = 3.42 ARR284 pKa = 11.84 SGDD287 pKa = 3.5 AVFDD291 pKa = 3.96 EE292 pKa = 4.5 VRR294 pKa = 11.84 QAVSSDD300 pKa = 3.45 VNFLAAIEE308 pKa = 4.64 KK309 pKa = 9.89 EE310 pKa = 4.18 YY311 pKa = 11.05 QPIEE315 pKa = 4.08 NVCLKK320 pKa = 10.02 EE321 pKa = 4.04 QIVQISMEE329 pKa = 4.56 LSRR332 pKa = 11.84 QRR334 pKa = 11.84 LKK336 pKa = 9.92 TAAPLLVLVDD346 pKa = 3.85 EE347 pKa = 4.64 DD348 pKa = 4.87 HH349 pKa = 7.2 EE350 pKa = 4.83 VTHH353 pKa = 7.07 LLAEE357 pKa = 4.26 NPKK360 pKa = 8.75 TAEE363 pKa = 4.12 KK364 pKa = 10.68 EE365 pKa = 4.25 LIFHH369 pKa = 6.31 FRR371 pKa = 11.84 VPEE374 pKa = 4.05 MEE376 pKa = 4.31 DD377 pKa = 3.15 VRR379 pKa = 11.84 IPSGAQEE386 pKa = 4.35 TFLPSAPPPGDD397 pKa = 3.43 PLFEE401 pKa = 4.29 TALGFPPNAISYY413 pKa = 9.2 EE414 pKa = 3.95 RR415 pKa = 11.84 DD416 pKa = 3.73 GIEE419 pKa = 3.58 GTLNHH424 pKa = 6.39 RR425 pKa = 11.84 PVYY428 pKa = 9.65 EE429 pKa = 4.15 KK430 pKa = 10.64 RR431 pKa = 11.84 PPTPEE436 pKa = 3.33 IFTPPEE442 pKa = 4.03 SPTTNSRR449 pKa = 11.84 PRR451 pKa = 11.84 WYY453 pKa = 9.73 DD454 pKa = 3.21 DD455 pKa = 4.6 KK456 pKa = 11.49 EE457 pKa = 4.47 IEE459 pKa = 4.3 DD460 pKa = 3.88 WSFPSPPDD468 pKa = 3.26 SPGFSEE474 pKa = 5.57 PEE476 pKa = 3.85 APPAKK481 pKa = 9.79 PPVSTRR487 pKa = 11.84 PIPPDD492 pKa = 3.28 LVLSPRR498 pKa = 11.84 DD499 pKa = 3.72 AYY501 pKa = 10.04 PVDD504 pKa = 3.75 EE505 pKa = 5.56 GGLGPIPEE513 pKa = 4.56 EE514 pKa = 4.33 GPLRR518 pKa = 11.84 SLVRR522 pKa = 11.84 RR523 pKa = 11.84 SLLSLLEE530 pKa = 4.27 KK531 pKa = 10.3 IRR533 pKa = 11.84 ALRR536 pKa = 11.84 RR537 pKa = 11.84 FAQKK541 pKa = 9.46 TGEE544 pKa = 4.17 SLLTDD549 pKa = 3.62 IQRR552 pKa = 11.84 IKK554 pKa = 11.06 LFLRR558 pKa = 4.14
Molecular weight: 61.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.088
IPC2_protein 5.054
IPC_protein 5.003
Toseland 4.902
ProMoST 5.105
Dawson 4.952
Bjellqvist 5.092
Wikipedia 4.787
Rodwell 4.863
Grimsley 4.813
Solomon 4.952
Lehninger 4.902
Nozaki 5.054
DTASelect 5.181
Thurlkill 4.889
EMBOSS 4.825
Sillero 5.13
Patrickios 4.418
IPC_peptide 4.952
IPC2_peptide 5.13
IPC2.peptide.svr19 5.112
Protein with the highest isoelectric point:
>tr|Q5ZR74|Q5ZR74_9ALPH Thymidine kinase (Fragment) OS=Hawaiian green turtle herpesvirus OX=70564 GN=UL23 PE=3 SV=2
MM1 pKa = 6.76 NTLRR5 pKa = 11.84 TRR7 pKa = 11.84 GARR10 pKa = 11.84 TSAFDD15 pKa = 3.7 YY16 pKa = 9.7 TSSRR20 pKa = 11.84 AVKK23 pKa = 9.99 KK24 pKa = 10.29 RR25 pKa = 11.84 RR26 pKa = 11.84 LLAGVRR32 pKa = 11.84 CHH34 pKa = 5.96 EE35 pKa = 4.12 RR36 pKa = 11.84 FYY38 pKa = 11.8 NLLTRR43 pKa = 11.84 GSQQINAGRR52 pKa = 11.84 PGAAAARR59 pKa = 11.84 PLVRR63 pKa = 11.84 ALGAAVDD70 pKa = 4.1 CRR72 pKa = 11.84 RR73 pKa = 11.84 EE74 pKa = 3.91 IIKK77 pKa = 9.52 TGVICEE83 pKa = 3.98 IDD85 pKa = 3.58 LGPRR89 pKa = 11.84 RR90 pKa = 11.84 PDD92 pKa = 2.99 CVIYY96 pKa = 10.44 VRR98 pKa = 11.84 RR99 pKa = 11.84 ASPVPHH105 pKa = 6.98 LLFLVEE111 pKa = 5.36 LKK113 pKa = 10.77 SCLHH117 pKa = 5.7 TRR119 pKa = 11.84 DD120 pKa = 3.01 MATRR124 pKa = 11.84 TKK126 pKa = 10.77 ALQRR130 pKa = 11.84 QEE132 pKa = 4.61 GLGQLRR138 pKa = 11.84 DD139 pKa = 3.46 ARR141 pKa = 11.84 ACLGRR146 pKa = 11.84 LTPPGPEE153 pKa = 4.13 PLLICPVLIFVAQKK167 pKa = 10.32 GLRR170 pKa = 11.84 ILAVQRR176 pKa = 11.84 LPTVAASVHH185 pKa = 5.87 FDD187 pKa = 2.94 RR188 pKa = 11.84 LLRR191 pKa = 11.84 VLLGRR196 pKa = 11.84 SCYY199 pKa = 9.85 HH200 pKa = 5.91 VRR202 pKa = 11.84 TAPGSPLDD210 pKa = 3.9 ARR212 pKa = 11.84 SQRR215 pKa = 11.84 SGARR219 pKa = 11.84 GVAQPAKK226 pKa = 9.58 PAAPSKK232 pKa = 10.58 LPSIPRR238 pKa = 11.84 SRR240 pKa = 11.84 SNPRR244 pKa = 11.84 SAGPRR249 pKa = 11.84 RR250 pKa = 11.84 SLAGQRR256 pKa = 11.84 RR257 pKa = 11.84 AARR260 pKa = 11.84 PKK262 pKa = 9.03 VRR264 pKa = 11.84 GAGSHH269 pKa = 4.78 GTAGGKK275 pKa = 9.58 HH276 pKa = 5.77 FDD278 pKa = 3.9 HH279 pKa = 7.8 RR280 pKa = 11.84 SGSNNAAGRR289 pKa = 11.84 AGRR292 pKa = 11.84 GRR294 pKa = 11.84 FKK296 pKa = 10.86 NRR298 pKa = 11.84 RR299 pKa = 11.84 GPGSRR304 pKa = 11.84 AGGRR308 pKa = 11.84 ALPANN313 pKa = 4.1
Molecular weight: 33.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.156
IPC2_protein 10.277
IPC_protein 11.754
Toseland 11.915
ProMoST 12.398
Dawson 11.915
Bjellqvist 11.901
Wikipedia 12.384
Rodwell 11.579
Grimsley 11.945
Solomon 12.398
Lehninger 12.296
Nozaki 11.915
DTASelect 11.901
Thurlkill 11.915
EMBOSS 12.398
Sillero 11.915
Patrickios 11.286
IPC_peptide 12.398
IPC2_peptide 11.389
IPC2.peptide.svr19 9.423
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
7492
111
1091
499.5
55.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.797 ± 0.568
1.962 ± 0.265
4.979 ± 0.335
6.887 ± 0.439
4.805 ± 0.241
6.1 ± 0.377
1.895 ± 0.162
2.803 ± 0.249
3.991 ± 0.253
10.918 ± 0.601
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.522 ± 0.165
3.217 ± 0.359
6.313 ± 0.814
3.31 ± 0.193
8.649 ± 0.539
6.38 ± 0.375
5.539 ± 0.294
6.874 ± 0.37
0.921 ± 0.127
3.137 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here