Escherichia phage vB_Eco_SLUR26

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Seuratvirus; unclassified Seuratvirus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3B1E5U3|A0A3B1E5U3_9CAUD Uncharacterized protein OS=Escherichia phage vB_Eco_SLUR26 OX=2029173 GN=SLURMMXVI_30001 PE=4 SV=1
MM1 pKa = 7.36RR2 pKa = 11.84VEE4 pKa = 4.48EE5 pKa = 4.01IVEE8 pKa = 4.13GKK10 pKa = 9.87EE11 pKa = 3.61YY12 pKa = 10.96RR13 pKa = 11.84LLTDD17 pKa = 3.43IGGVGYY23 pKa = 8.53TPDD26 pKa = 3.2NAACFNVLPQGAIVTVNKK44 pKa = 9.97VVTDD48 pKa = 4.39LYY50 pKa = 10.96SVHH53 pKa = 7.46DD54 pKa = 4.51GRR56 pKa = 11.84WPHH59 pKa = 5.98AVIVTFGKK67 pKa = 10.28DD68 pKa = 3.12GYY70 pKa = 11.32VDD72 pKa = 5.34LDD74 pKa = 4.32GEE76 pKa = 4.64DD77 pKa = 3.45DD78 pKa = 4.01TYY80 pKa = 11.68EE81 pKa = 3.86VAYY84 pKa = 9.28TGLVSPNFLEE94 pKa = 4.59EE95 pKa = 4.0VV96 pKa = 3.43

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3B1E3E3|A0A3B1E3E3_9CAUD Uncharacterized protein OS=Escherichia phage vB_Eco_SLUR26 OX=2029173 GN=SLURMMXVI_30004 PE=4 SV=1
MM1 pKa = 6.88FHH3 pKa = 7.16IFLFLLLQSFEE14 pKa = 4.16AAFDD18 pKa = 3.63DD19 pKa = 3.9FRR21 pKa = 11.84IYY23 pKa = 10.69HH24 pKa = 6.35RR25 pKa = 11.84AQYY28 pKa = 10.51RR29 pKa = 11.84DD30 pKa = 3.28AGSWRR35 pKa = 11.84GGRR38 pKa = 11.84CRR40 pKa = 11.84YY41 pKa = 9.19FLLRR45 pKa = 11.84LLRR48 pKa = 11.84FFMVAQHH55 pKa = 6.67LKK57 pKa = 10.66HH58 pKa = 6.55FLLDD62 pKa = 3.76DD63 pKa = 3.88VKK65 pKa = 10.51TDD67 pKa = 3.72LVLMDD72 pKa = 4.31KK73 pKa = 11.1LFVCVACNLTPTGLKK88 pKa = 8.88GTACIRR94 pKa = 11.84FHH96 pKa = 6.51GVAHH100 pKa = 6.98FILFAVSYY108 pKa = 8.83FRR110 pKa = 11.84HH111 pKa = 6.45GIAPFINISLLITAHH126 pKa = 7.12RR127 pKa = 11.84NDD129 pKa = 3.6EE130 pKa = 3.9QCIRR134 pKa = 11.84KK135 pKa = 9.65LIARR139 pKa = 11.84LLRR142 pKa = 11.84NRR144 pKa = 11.84RR145 pKa = 11.84LRR147 pKa = 11.84YY148 pKa = 8.41PCLRR152 pKa = 11.84KK153 pKa = 9.21QRR155 pKa = 11.84CYY157 pKa = 10.91SRR159 pKa = 11.84CRR161 pKa = 11.84CPRR164 pKa = 3.25

Molecular weight: 19.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

18348

38

1040

199.4

22.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.156 ± 0.58

1.461 ± 0.158

5.652 ± 0.177

6.399 ± 0.206

3.613 ± 0.182

7.183 ± 0.232

1.864 ± 0.177

5.892 ± 0.188

6.224 ± 0.269

7.565 ± 0.246

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.861 ± 0.177

4.954 ± 0.171

4.175 ± 0.265

4.333 ± 0.29

5.036 ± 0.193

5.537 ± 0.275

5.924 ± 0.303

7.02 ± 0.18

1.559 ± 0.131

3.592 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski