Pseudomonas phage vB_PaeS_SCUT-S3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Septimatrevirus; unclassified Septimatrevirus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8G5V9|A0A3S8G5V9_9CAUD Virion structural protein OS=Pseudomonas phage vB_PaeS_SCUT-S3 OX=2382122 GN=S3_016 PE=4 SV=1
MM1 pKa = 7.02ATKK4 pKa = 10.28KK5 pKa = 10.42VVYY8 pKa = 8.13FTAGINATAGEE19 pKa = 4.19LADD22 pKa = 3.58IAKK25 pKa = 10.21LNAAAEE31 pKa = 4.15PQYY34 pKa = 11.14EE35 pKa = 4.21VLVANGAANAEE46 pKa = 4.2YY47 pKa = 10.98GEE49 pKa = 4.29TDD51 pKa = 4.17RR52 pKa = 11.84IIPADD57 pKa = 4.88FIAGTIPDD65 pKa = 4.19VYY67 pKa = 10.66RR68 pKa = 11.84VDD70 pKa = 4.08GDD72 pKa = 3.87PLEE75 pKa = 5.13DD76 pKa = 3.3PVYY79 pKa = 10.9PEE81 pKa = 4.97IDD83 pKa = 3.33PDD85 pKa = 4.74AIPNQALTATQVIVNDD101 pKa = 3.97AEE103 pKa = 4.23ALTVPVTGTYY113 pKa = 7.23TTTATVSVANGVVTGIVLSS132 pKa = 3.99

Molecular weight: 13.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8KTU2|A0A3Q8KTU2_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeS_SCUT-S3 OX=2382122 GN=S3_036 PE=4 SV=1
MM1 pKa = 7.33NALNNIQRR9 pKa = 11.84VVSWNSKK16 pKa = 10.03KK17 pKa = 9.49NTDD20 pKa = 3.31GTFTATAYY28 pKa = 10.93SFDD31 pKa = 4.03HH32 pKa = 6.2NAPTVVHH39 pKa = 6.76ASYY42 pKa = 10.09TRR44 pKa = 11.84ASRR47 pKa = 11.84AQAVADD53 pKa = 3.64AKK55 pKa = 10.92KK56 pKa = 10.26AVRR59 pKa = 11.84YY60 pKa = 9.57LKK62 pKa = 10.36AQQRR66 pKa = 11.84TAA68 pKa = 3.26

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

13418

49

1201

216.4

23.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.567 ± 0.774

1.021 ± 0.172

5.321 ± 0.216

6.618 ± 0.336

3.741 ± 0.182

7.654 ± 0.344

1.327 ± 0.168

5.031 ± 0.211

5.455 ± 0.387

7.877 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.079 ± 0.114

4.852 ± 0.179

4.919 ± 0.363

4.032 ± 0.553

5.351 ± 0.233

5.761 ± 0.25

5.694 ± 0.318

6.842 ± 0.315

1.55 ± 0.146

3.309 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski