Amino acid dipepetide frequency for Rhizobium sp. BK251

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.753AlaAla: 15.753 ± 0.134	0.933AlaCys: 0.933 ± 0.025	6.52AlaAsp: 6.52 ± 0.06	7.382AlaGlu: 7.382 ± 0.07	4.624AlaPhe: 4.624 ± 0.049	10.02AlaGly: 10.02 ± 0.089	2.125AlaHis: 2.125 ± 0.033	6.947AlaIle: 6.947 ± 0.07	4.251AlaLys: 4.251 ± 0.052	12.209AlaLeu: 12.209 ± 0.092	3.363AlaMet: 3.363 ± 0.043	3.079AlaAsn: 3.079 ± 0.042	4.741AlaPro: 4.741 ± 0.06	3.415AlaGln: 3.415 ± 0.05	7.861AlaArg: 7.861 ± 0.075	6.768AlaSer: 6.768 ± 0.06	5.922AlaThr: 5.922 ± 0.058	8.608AlaVal: 8.608 ± 0.08	1.4AlaTrp: 1.4 ± 0.028	2.552AlaTyr: 2.552 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.829CysAla: 0.829 ± 0.019	0.109CysCys: 0.109 ± 0.008	0.486CysAsp: 0.486 ± 0.015	0.428CysGlu: 0.428 ± 0.016	0.316CysPhe: 0.316 ± 0.013	0.865CysGly: 0.865 ± 0.022	0.215CysHis: 0.215 ± 0.011	0.419CysIle: 0.419 ± 0.016	0.174CysLys: 0.174 ± 0.009	0.817CysLeu: 0.817 ± 0.02	0.165CysMet: 0.165 ± 0.009	0.193CysAsn: 0.193 ± 0.011	0.373CysPro: 0.373 ± 0.015	0.207CysGln: 0.207 ± 0.011	0.602CysArg: 0.602 ± 0.019	0.457CysSer: 0.457 ± 0.016	0.392CysThr: 0.392 ± 0.016	0.562CysVal: 0.562 ± 0.017	0.105CysTrp: 0.105 ± 0.007	0.186CysTyr: 0.186 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.311AspAla: 6.311 ± 0.064	0.472AspCys: 0.472 ± 0.015	3.155AspAsp: 3.155 ± 0.055	3.594AspGlu: 3.594 ± 0.041	2.301AspPhe: 2.301 ± 0.033	5.099AspGly: 5.099 ± 0.056	1.277AspHis: 1.277 ± 0.032	3.418AspIle: 3.418 ± 0.039	1.786AspLys: 1.786 ± 0.03	5.693AspLeu: 5.693 ± 0.061	1.313AspMet: 1.313 ± 0.025	1.492AspAsn: 1.492 ± 0.032	3.272AspPro: 3.272 ± 0.046	1.664AspGln: 1.664 ± 0.029	4.23AspArg: 4.23 ± 0.047	2.179AspSer: 2.179 ± 0.032	2.545AspThr: 2.545 ± 0.049	4.247AspVal: 4.247 ± 0.051	0.948AspTrp: 0.948 ± 0.023	1.544AspTyr: 1.544 ± 0.028	0.0AspXaa: 0.0 ± 0.0
Glu	7.379GluAla: 7.379 ± 0.073	0.353GluCys: 0.353 ± 0.015	2.848GluAsp: 2.848 ± 0.038	3.575GluGlu: 3.575 ± 0.051	1.964GluPhe: 1.964 ± 0.031	4.329GluGly: 4.329 ± 0.055	1.227GluHis: 1.227 ± 0.026	3.821GluIle: 3.821 ± 0.049	2.689GluLys: 2.689 ± 0.038	5.551GluLeu: 5.551 ± 0.06	1.576GluMet: 1.576 ± 0.03	1.834GluAsn: 1.834 ± 0.03	2.637GluPro: 2.637 ± 0.036	2.048GluGln: 2.048 ± 0.033	4.895GluArg: 4.895 ± 0.067	2.512GluSer: 2.512 ± 0.038	3.569GluThr: 3.569 ± 0.044	3.777GluVal: 3.777 ± 0.053	0.722GluTrp: 0.722 ± 0.021	1.13GluTyr: 1.13 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.609PheAla: 4.609 ± 0.056	0.402PheCys: 0.402 ± 0.013	2.688PheAsp: 2.688 ± 0.041	2.258PheGlu: 2.258 ± 0.036	1.604PhePhe: 1.604 ± 0.033	3.742PheGly: 3.742 ± 0.045	0.806PheHis: 0.806 ± 0.02	1.928PheIle: 1.928 ± 0.031	1.109PheLys: 1.109 ± 0.024	3.709PheLeu: 3.709 ± 0.049	0.877PheMet: 0.877 ± 0.02	1.203PheAsn: 1.203 ± 0.028	1.686PhePro: 1.686 ± 0.03	1.096PheGln: 1.096 ± 0.022	2.354PheArg: 2.354 ± 0.039	2.57PheSer: 2.57 ± 0.038	2.014PheThr: 2.014 ± 0.034	3.0PheVal: 3.0 ± 0.043	0.588PheTrp: 0.588 ± 0.017	1.007PheTyr: 1.007 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	8.344GlyAla: 8.344 ± 0.074	0.771GlyCys: 0.771 ± 0.023	4.355GlyAsp: 4.355 ± 0.056	4.837GlyGlu: 4.837 ± 0.046	3.627GlyPhe: 3.627 ± 0.046	7.025GlyGly: 7.025 ± 0.093	1.811GlyHis: 1.811 ± 0.033	5.047GlyIle: 5.047 ± 0.054	3.524GlyLys: 3.524 ± 0.052	8.568GlyLeu: 8.568 ± 0.081	2.308GlyMet: 2.308 ± 0.032	2.499GlyAsn: 2.499 ± 0.057	3.259GlyPro: 3.259 ± 0.043	2.743GlyGln: 2.743 ± 0.04	5.837GlyArg: 5.837 ± 0.056	4.945GlySer: 4.945 ± 0.047	4.534GlyThr: 4.534 ± 0.057	5.753GlyVal: 5.753 ± 0.059	1.279GlyTrp: 1.279 ± 0.027	2.411GlyTyr: 2.411 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.184HisAla: 2.184 ± 0.036	0.207HisCys: 0.207 ± 0.011	1.244HisAsp: 1.244 ± 0.029	1.148HisGlu: 1.148 ± 0.028	0.971HisPhe: 0.971 ± 0.025	1.9HisGly: 1.9 ± 0.03	0.56HisHis: 0.56 ± 0.02	1.049HisIle: 1.049 ± 0.023	0.471HisLys: 0.471 ± 0.016	2.151HisLeu: 2.151 ± 0.036	0.553HisMet: 0.553 ± 0.016	0.472HisAsn: 0.472 ± 0.014	1.259HisPro: 1.259 ± 0.031	0.575HisGln: 0.575 ± 0.017	1.401HisArg: 1.401 ± 0.025	1.036HisSer: 1.036 ± 0.022	0.787HisThr: 0.787 ± 0.02	1.533HisVal: 1.533 ± 0.029	0.336HisTrp: 0.336 ± 0.014	0.554HisTyr: 0.554 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	7.768IleAla: 7.768 ± 0.069	0.554IleCys: 0.554 ± 0.017	3.776IleAsp: 3.776 ± 0.041	3.759IleGlu: 3.759 ± 0.046	2.087IlePhe: 2.087 ± 0.035	5.238IleGly: 5.238 ± 0.057	0.992IleHis: 0.992 ± 0.021	2.777IleIle: 2.777 ± 0.04	1.605IleLys: 1.605 ± 0.029	4.977IleLeu: 4.977 ± 0.065	1.122IleMet: 1.122 ± 0.026	1.628IleAsn: 1.628 ± 0.035	2.547IlePro: 2.547 ± 0.036	1.287IleGln: 1.287 ± 0.027	3.478IleArg: 3.478 ± 0.038	3.504IleSer: 3.504 ± 0.046	2.798IleThr: 2.798 ± 0.036	4.686IleVal: 4.686 ± 0.052	0.662IleTrp: 0.662 ± 0.019	1.285IleTyr: 1.285 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	4.572LysAla: 4.572 ± 0.062	0.161LysCys: 0.161 ± 0.01	1.918LysAsp: 1.918 ± 0.036	1.92LysGlu: 1.92 ± 0.037	1.008LysPhe: 1.008 ± 0.02	2.791LysGly: 2.791 ± 0.044	0.633LysHis: 0.633 ± 0.015	1.95LysIle: 1.95 ± 0.032	1.407LysLys: 1.407 ± 0.03	3.553LysLeu: 3.553 ± 0.044	0.902LysMet: 0.902 ± 0.021	1.007LysAsn: 1.007 ± 0.022	2.097LysPro: 2.097 ± 0.033	1.139LysGln: 1.139 ± 0.025	2.411LysArg: 2.411 ± 0.037	2.101LysSer: 2.101 ± 0.036	2.114LysThr: 2.114 ± 0.037	2.612LysVal: 2.612 ± 0.04	0.407LysTrp: 0.407 ± 0.016	0.698LysTyr: 0.698 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	12.85LeuAla: 12.85 ± 0.107	0.851LeuCys: 0.851 ± 0.021	5.82LeuAsp: 5.82 ± 0.057	5.325LeuGlu: 5.325 ± 0.055	3.82LeuPhe: 3.82 ± 0.048	8.032LeuGly: 8.032 ± 0.087	1.841LeuHis: 1.841 ± 0.033	5.17LeuIle: 5.17 ± 0.073	3.865LeuLys: 3.865 ± 0.046	9.586LeuLeu: 9.586 ± 0.096	2.348LeuMet: 2.348 ± 0.036	2.637LeuAsn: 2.637 ± 0.043	5.304LeuPro: 5.304 ± 0.054	2.974LeuGln: 2.974 ± 0.04	6.42LeuArg: 6.42 ± 0.069	6.984LeuSer: 6.984 ± 0.06	5.388LeuThr: 5.388 ± 0.054	7.589LeuVal: 7.589 ± 0.071	1.118LeuTrp: 1.118 ± 0.026	2.102LeuTyr: 2.102 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	2.973MetAla: 2.973 ± 0.04	0.133MetCys: 0.133 ± 0.009	1.182MetAsp: 1.182 ± 0.025	1.218MetGlu: 1.218 ± 0.022	0.712MetPhe: 0.712 ± 0.021	1.704MetGly: 1.704 ± 0.027	0.477MetHis: 0.477 ± 0.015	1.51MetIle: 1.51 ± 0.026	1.097MetLys: 1.097 ± 0.026	2.539MetLeu: 2.539 ± 0.039	0.691MetMet: 0.691 ± 0.018	0.875MetAsn: 0.875 ± 0.023	1.539MetPro: 1.539 ± 0.028	0.87MetGln: 0.87 ± 0.02	1.813MetArg: 1.813 ± 0.033	1.752MetSer: 1.752 ± 0.032	1.944MetThr: 1.944 ± 0.032	1.73MetVal: 1.73 ± 0.027	0.194MetTrp: 0.194 ± 0.011	0.346MetTyr: 0.346 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.306AsnAla: 3.306 ± 0.044	0.251AsnCys: 0.251 ± 0.012	1.629AsnAsp: 1.629 ± 0.042	1.432AsnGlu: 1.432 ± 0.03	1.144AsnPhe: 1.144 ± 0.027	2.601AsnGly: 2.601 ± 0.044	0.562AsnHis: 0.562 ± 0.02	1.594AsnIle: 1.594 ± 0.027	0.8AsnLys: 0.8 ± 0.02	2.832AsnLeu: 2.832 ± 0.045	0.723AsnMet: 0.723 ± 0.018	0.805AsnAsn: 0.805 ± 0.023	1.867AsnPro: 1.867 ± 0.028	0.826AsnGln: 0.826 ± 0.021	1.921AsnArg: 1.921 ± 0.035	1.522AsnSer: 1.522 ± 0.03	1.335AsnThr: 1.335 ± 0.033	2.112AsnVal: 2.112 ± 0.036	0.483AsnTrp: 0.483 ± 0.016	0.722AsnTyr: 0.722 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	5.639ProAla: 5.639 ± 0.064	0.286ProCys: 0.286 ± 0.013	3.402ProAsp: 3.402 ± 0.045	3.571ProGlu: 3.571 ± 0.047	1.976ProPhe: 1.976 ± 0.032	4.133ProGly: 4.133 ± 0.048	1.05ProHis: 1.05 ± 0.023	2.398ProIle: 2.398 ± 0.038	1.709ProLys: 1.709 ± 0.031	4.645ProLeu: 4.645 ± 0.054	1.14ProMet: 1.14 ± 0.025	1.422ProAsn: 1.422 ± 0.028	2.329ProPro: 2.329 ± 0.042	1.645ProGln: 1.645 ± 0.029	2.647ProArg: 2.647 ± 0.04	2.965ProSer: 2.965 ± 0.046	2.401ProThr: 2.401 ± 0.035	4.07ProVal: 4.07 ± 0.047	0.638ProTrp: 0.638 ± 0.023	1.223ProTyr: 1.223 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.781GlnAla: 3.781 ± 0.047	0.16GlnCys: 0.16 ± 0.01	1.455GlnAsp: 1.455 ± 0.029	1.572GlnGlu: 1.572 ± 0.031	1.117GlnPhe: 1.117 ± 0.023	2.272GlnGly: 2.272 ± 0.035	0.618GlnHis: 0.618 ± 0.016	1.866GlnIle: 1.866 ± 0.033	1.218GlnLys: 1.218 ± 0.027	2.904GlnLeu: 2.904 ± 0.042	0.926GlnMet: 0.926 ± 0.021	0.896GlnAsn: 0.896 ± 0.021	1.747GlnPro: 1.747 ± 0.034	1.331GlnGln: 1.331 ± 0.031	2.24GlnArg: 2.24 ± 0.04	1.894GlnSer: 1.894 ± 0.037	1.668GlnThr: 1.668 ± 0.03	2.14GlnVal: 2.14 ± 0.034	0.381GlnTrp: 0.381 ± 0.015	0.657GlnTyr: 0.657 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	6.961ArgAla: 6.961 ± 0.071	0.452ArgCys: 0.452 ± 0.016	3.803ArgAsp: 3.803 ± 0.041	4.156ArgGlu: 4.156 ± 0.048	2.874ArgPhe: 2.874 ± 0.04	4.508ArgGly: 4.508 ± 0.057	1.693ArgHis: 1.693 ± 0.033	4.091ArgIle: 4.091 ± 0.052	2.499ArgLys: 2.499 ± 0.037	7.506ArgLeu: 7.506 ± 0.075	1.888ArgMet: 1.888 ± 0.032	2.108ArgAsn: 2.108 ± 0.032	3.284ArgPro: 3.284 ± 0.047	2.61ArgGln: 2.61 ± 0.041	5.369ArgArg: 5.369 ± 0.069	4.041ArgSer: 4.041 ± 0.054	3.234ArgThr: 3.234 ± 0.042	4.329ArgVal: 4.329 ± 0.044	0.946ArgTrp: 0.946 ± 0.025	1.71ArgTyr: 1.71 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.496SerAla: 6.496 ± 0.058	0.423SerCys: 0.423 ± 0.014	3.226SerAsp: 3.226 ± 0.045	3.196SerGlu: 3.196 ± 0.045	2.536SerPhe: 2.536 ± 0.043	5.786SerGly: 5.786 ± 0.063	1.208SerHis: 1.208 ± 0.028	3.328SerIle: 3.328 ± 0.041	1.838SerLys: 1.838 ± 0.032	5.954SerLeu: 5.954 ± 0.066	1.455SerMet: 1.455 ± 0.03	1.657SerAsn: 1.657 ± 0.031	2.942SerPro: 2.942 ± 0.044	1.702SerGln: 1.702 ± 0.031	3.901SerArg: 3.901 ± 0.05	3.46SerSer: 3.46 ± 0.053	2.946SerThr: 2.946 ± 0.041	4.361SerVal: 4.361 ± 0.053	0.802SerTrp: 0.802 ± 0.022	1.417SerTyr: 1.417 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	6.09ThrAla: 6.09 ± 0.059	0.373ThrCys: 0.373 ± 0.014	2.771ThrAsp: 2.771 ± 0.039	2.711ThrGlu: 2.711 ± 0.038	2.091ThrPhe: 2.091 ± 0.035	4.965ThrGly: 4.965 ± 0.058	1.019ThrHis: 1.019 ± 0.025	3.25ThrIle: 3.25 ± 0.043	1.674ThrLys: 1.674 ± 0.031	5.538ThrLeu: 5.538 ± 0.063	1.256ThrMet: 1.256 ± 0.024	1.416ThrAsn: 1.416 ± 0.028	2.93ThrPro: 2.93 ± 0.036	1.395ThrGln: 1.395 ± 0.029	3.079ThrArg: 3.079 ± 0.039	2.959ThrSer: 2.959 ± 0.041	2.912ThrThr: 2.912 ± 0.04	4.377ThrVal: 4.377 ± 0.051	0.627ThrTrp: 0.627 ± 0.018	1.255ThrTyr: 1.255 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	8.965ValAla: 8.965 ± 0.078	0.62ValCys: 0.62 ± 0.018	4.139ValAsp: 4.139 ± 0.049	4.443ValGlu: 4.443 ± 0.047	2.92ValPhe: 2.92 ± 0.042	5.461ValGly: 5.461 ± 0.052	1.418ValHis: 1.418 ± 0.027	4.224ValIle: 4.224 ± 0.047	2.464ValLys: 2.464 ± 0.041	7.352ValLeu: 7.352 ± 0.076	1.85ValMet: 1.85 ± 0.031	2.076ValAsn: 2.076 ± 0.033	3.66ValPro: 3.66 ± 0.049	2.019ValGln: 2.019 ± 0.031	4.774ValArg: 4.774 ± 0.058	4.807ValSer: 4.807 ± 0.054	4.357ValThr: 4.357 ± 0.044	5.976ValVal: 5.976 ± 0.058	0.856ValTrp: 0.856 ± 0.022	1.531ValTyr: 1.531 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.179TrpAla: 1.179 ± 0.027	0.138TrpCys: 0.138 ± 0.008	0.647TrpAsp: 0.647 ± 0.02	0.602TrpGlu: 0.602 ± 0.018	0.523TrpPhe: 0.523 ± 0.017	0.819TrpGly: 0.819 ± 0.02	0.323TrpHis: 0.323 ± 0.013	0.704TrpIle: 0.704 ± 0.022	0.507TrpLys: 0.507 ± 0.016	1.601TrpLeu: 1.601 ± 0.031	0.361TrpMet: 0.361 ± 0.015	0.518TrpAsn: 0.518 ± 0.017	0.687TrpPro: 0.687 ± 0.02	0.557TrpGln: 0.557 ± 0.016	1.033TrpArg: 1.033 ± 0.023	0.863TrpSer: 0.863 ± 0.022	0.763TrpThr: 0.763 ± 0.021	0.757TrpVal: 0.757 ± 0.022	0.218TrpTrp: 0.218 ± 0.012	0.302TrpTyr: 0.302 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.483TyrAla: 2.483 ± 0.039	0.249TyrCys: 0.249 ± 0.011	1.475TyrAsp: 1.475 ± 0.029	1.311TyrGlu: 1.311 ± 0.024	1.038TyrPhe: 1.038 ± 0.022	2.186TyrGly: 2.186 ± 0.035	0.536TyrHis: 0.536 ± 0.018	1.039TyrIle: 1.039 ± 0.025	0.716TyrLys: 0.716 ± 0.019	2.339TyrLeu: 2.339 ± 0.033	0.483TyrMet: 0.483 ± 0.015	0.648TyrAsn: 0.648 ± 0.017	1.129TyrPro: 1.129 ± 0.023	0.737TyrGln: 0.737 ± 0.021	1.797TyrArg: 1.797 ± 0.031	1.313TyrSer: 1.313 ± 0.026	1.065TyrThr: 1.065 ± 0.026	1.695TyrVal: 1.695 ± 0.03	0.393TyrTrp: 0.393 ± 0.015	0.65TyrTyr: 0.65 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6346 proteins (1948081 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---