Streptococcus phage Javan124

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6API8|A0A4D6API8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan124 OX=2547991 GN=Javan124_0025 PE=4 SV=1
MM1 pKa = 7.63PVGLIPKK8 pKa = 8.75EE9 pKa = 3.78ILVTSVGCDD18 pKa = 3.25MVNDD22 pKa = 4.12CCLGQLTFGFALNTEE37 pKa = 4.13WMNFF41 pKa = 3.37

Molecular weight: 4.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AU82|A0A4D6AU82_9CAUD Uncharacterized protein OS=Streptococcus phage Javan124 OX=2547991 GN=Javan124_0028 PE=4 SV=1
MM1 pKa = 7.62LGLPSRR7 pKa = 11.84ILRR10 pKa = 11.84LVGFGFRR17 pKa = 11.84PLTAMAITSSSATKK31 pKa = 10.18KK32 pKa = 10.56GLRR35 pKa = 11.84TFF37 pKa = 4.07

Molecular weight: 4.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

12015

37

971

293.0

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.525 ± 0.559

0.807 ± 0.13

5.718 ± 0.336

7.308 ± 0.36

4.02 ± 0.295

6.717 ± 0.361

1.798 ± 0.147

6.475 ± 0.404

7.05 ± 0.259

9.097 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.177

4.802 ± 0.288

3.104 ± 0.22

4.236 ± 0.217

4.711 ± 0.274

7.133 ± 0.383

6.484 ± 0.437

6.475 ± 0.249

1.398 ± 0.101

3.737 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski