Flavobacterium phage vB_FspP_elemoA_2-5C

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7IXF0|A0A7D7IXF0_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspP_elemoA_2-5C OX=2743815 GN=elemo25C_phanotate12 PE=4 SV=1
MM1 pKa = 7.43ARR3 pKa = 11.84IKK5 pKa = 10.03TYY7 pKa = 10.42TIDD10 pKa = 3.75TLISDD15 pKa = 3.92NDD17 pKa = 3.88IIIGSDD23 pKa = 2.83ADD25 pKa = 3.98NNNEE29 pKa = 4.18TKK31 pKa = 10.68NFSAGLIRR39 pKa = 11.84EE40 pKa = 4.38FVLSGLEE47 pKa = 4.02PEE49 pKa = 4.48VGGNLKK55 pKa = 8.7ITTIVDD61 pKa = 3.24NDD63 pKa = 3.97SEE65 pKa = 4.56EE66 pKa = 4.43TTPEE70 pKa = 3.99DD71 pKa = 3.59YY72 pKa = 10.94FNNSVTPIIVLHH84 pKa = 5.26YY85 pKa = 9.83EE86 pKa = 3.83IVFLILNGRR95 pKa = 11.84TFIFRR100 pKa = 11.84KK101 pKa = 10.51NNDD104 pKa = 3.08TYY106 pKa = 11.57GVDD109 pKa = 3.37EE110 pKa = 4.44TQVVSGDD117 pKa = 3.4FTEE120 pKa = 4.8IDD122 pKa = 3.08ITSVINANLQDD133 pKa = 4.38LDD135 pKa = 4.02SVLTEE140 pKa = 4.25GNEE143 pKa = 4.8APDD146 pKa = 3.64KK147 pKa = 10.42DD148 pKa = 3.84AKK150 pKa = 10.74IRR152 pKa = 11.84DD153 pKa = 3.64LYY155 pKa = 11.22LYY157 pKa = 10.55DD158 pKa = 5.55DD159 pKa = 4.97FGSPDD164 pKa = 3.27GYY166 pKa = 11.61ARR168 pKa = 11.84LYY170 pKa = 10.82SDD172 pKa = 3.34KK173 pKa = 10.93QSLYY177 pKa = 10.94FMNKK181 pKa = 7.41TGEE184 pKa = 4.39SVFQLEE190 pKa = 4.51KK191 pKa = 10.67NSLYY195 pKa = 10.65FPIGAYY201 pKa = 7.89TYY203 pKa = 10.67KK204 pKa = 9.39ITTPSITGGRR214 pKa = 11.84IATFQDD220 pKa = 2.86ASGIVAYY227 pKa = 9.58TSDD230 pKa = 3.7IPTDD234 pKa = 4.03YY235 pKa = 10.41ISSITSSSDD244 pKa = 2.96EE245 pKa = 4.02LDD247 pKa = 3.08ITTVAGEE254 pKa = 4.03TTIIYY259 pKa = 8.06TPKK262 pKa = 10.58KK263 pKa = 9.09EE264 pKa = 3.77IQLIGTEE271 pKa = 4.25LSNNLTNTTFFSNGGEE287 pKa = 3.9FSYY290 pKa = 11.47DD291 pKa = 2.99EE292 pKa = 4.13TLYY295 pKa = 10.5PITALDD301 pKa = 3.86LKK303 pKa = 10.84FNYY306 pKa = 10.62NIDD309 pKa = 3.72DD310 pKa = 3.73NGIDD314 pKa = 3.25FGYY317 pKa = 11.01YY318 pKa = 8.69VFHH321 pKa = 7.66YY322 pKa = 10.39EE323 pKa = 4.68DD324 pKa = 3.95GRR326 pKa = 11.84NVIAPIEE333 pKa = 3.91LAGSIISGTEE343 pKa = 3.29LRR345 pKa = 11.84VRR347 pKa = 11.84LDD349 pKa = 3.19LATYY353 pKa = 6.96WTEE356 pKa = 4.13SPDD359 pKa = 3.24PRR361 pKa = 11.84LSYY364 pKa = 10.93FEE366 pKa = 4.47INLYY370 pKa = 10.15QGTLISEE377 pKa = 4.53DD378 pKa = 3.04GVYY381 pKa = 10.6FDD383 pKa = 4.44GHH385 pKa = 6.54APSSQIKK392 pKa = 9.75YY393 pKa = 9.9FNIWTT398 pKa = 3.82

Molecular weight: 44.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7F4W6|A0A7D7F4W6_9CAUD Structural protein OS=Flavobacterium phage vB_FspP_elemoA_2-5C OX=2743815 GN=elemo25C_phanotate87 PE=4 SV=1
MM1 pKa = 7.51AEE3 pKa = 4.22SVKK6 pKa = 11.2SNMKK10 pKa = 10.01CGEE13 pKa = 4.04VRR15 pKa = 11.84KK16 pKa = 8.56STRR19 pKa = 11.84PSKK22 pKa = 10.82KK23 pKa = 9.54IMKK26 pKa = 9.37KK27 pKa = 8.48YY28 pKa = 9.9CVNGKK33 pKa = 7.42EE34 pKa = 4.37TLVHH38 pKa = 6.88AGAKK42 pKa = 9.88GYY44 pKa = 11.24SDD46 pKa = 3.55FTKK49 pKa = 10.72HH50 pKa = 6.71KK51 pKa = 10.3DD52 pKa = 3.3SKK54 pKa = 10.2RR55 pKa = 11.84KK56 pKa = 9.37KK57 pKa = 9.71NFHH60 pKa = 6.63ARR62 pKa = 11.84HH63 pKa = 5.67KK64 pKa = 10.58CATAKK69 pKa = 10.52PGTPKK74 pKa = 10.47HH75 pKa = 5.56LACTKK80 pKa = 10.28LWW82 pKa = 3.54

Molecular weight: 9.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

19296

30

3694

212.0

24.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.353 ± 0.404

0.762 ± 0.156

6.939 ± 0.191

7.4 ± 0.332

4.54 ± 0.221

5.835 ± 0.249

1.182 ± 0.167

7.634 ± 0.228

9.432 ± 0.684

7.815 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.254 ± 0.162

7.24 ± 0.184

2.684 ± 0.134

3.063 ± 0.251

3.555 ± 0.155

7.204 ± 0.213

5.675 ± 0.316

5.949 ± 0.249

0.876 ± 0.119

4.607 ± 0.345
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski