Dragonfly associated cyclovirus 5
Average proteome isoelectric point is 8.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K0A152|K0A152_9CIRC Putative capsid protein OS=Dragonfly associated cyclovirus 5 OX=1234883 PE=4 SV=1
MM1 pKa = 7.49 ANCVVRR7 pKa = 11.84 RR8 pKa = 11.84 FCFTLNNFTEE18 pKa = 4.23 EE19 pKa = 4.34 EE20 pKa = 4.46 YY21 pKa = 11.11 EE22 pKa = 4.05 KK23 pKa = 10.17 VTRR26 pKa = 11.84 FIQDD30 pKa = 3.06 YY31 pKa = 9.34 CKK33 pKa = 10.81 YY34 pKa = 10.13 GIVGNEE40 pKa = 3.89 TAPTTGTIHH49 pKa = 7.45 LPGFCNLTKK58 pKa = 10.52 GMRR61 pKa = 11.84 FNNIKK66 pKa = 10.5 SKK68 pKa = 10.37 LATRR72 pKa = 11.84 IHH74 pKa = 6.51 LEE76 pKa = 3.96 KK77 pKa = 11.17 ANGTDD82 pKa = 3.41 EE83 pKa = 4.22 QNQIYY88 pKa = 9.43 CRR90 pKa = 11.84 KK91 pKa = 9.5 SGTYY95 pKa = 9.25 FEE97 pKa = 5.43 KK98 pKa = 10.26 GTPVGQGKK106 pKa = 7.85 RR107 pKa = 11.84 TDD109 pKa = 3.41 LVSLVEE115 pKa = 5.0 GIQNGQIRR123 pKa = 11.84 LSDD126 pKa = 3.27 IAKK129 pKa = 9.21 DD130 pKa = 3.51 HH131 pKa = 7.14 PIAFIKK137 pKa = 7.71 YY138 pKa = 7.66 HH139 pKa = 6.07 RR140 pKa = 11.84 GIRR143 pKa = 11.84 EE144 pKa = 3.8 YY145 pKa = 11.1 LQLTKK150 pKa = 10.21 PVQPRR155 pKa = 11.84 MFKK158 pKa = 8.93 TWVYY162 pKa = 9.53 YY163 pKa = 9.52 YY164 pKa = 9.7 WGPTGSGKK172 pKa = 9.98 SSRR175 pKa = 11.84 ALKK178 pKa = 10.0 EE179 pKa = 3.61 AMEE182 pKa = 4.5 IEE184 pKa = 3.96 GEE186 pKa = 4.07 IYY188 pKa = 10.52 YY189 pKa = 10.25 KK190 pKa = 10.56 PRR192 pKa = 11.84 GLWWDD197 pKa = 4.71 GYY199 pKa = 9.4 HH200 pKa = 5.68 QQDD203 pKa = 3.74 NVIIDD208 pKa = 4.59 DD209 pKa = 4.41 FYY211 pKa = 11.73 GWIKK215 pKa = 9.81 YY216 pKa = 10.71 DD217 pKa = 3.64 EE218 pKa = 4.36 MLKK221 pKa = 10.5 IMDD224 pKa = 4.59 RR225 pKa = 11.84 YY226 pKa = 9.01 PYY228 pKa = 9.88 KK229 pKa = 10.71 VQVKK233 pKa = 10.29 GGFEE237 pKa = 4.2 EE238 pKa = 4.82 FTSRR242 pKa = 11.84 RR243 pKa = 11.84 IWITSNVDD251 pKa = 2.59 TDD253 pKa = 3.55 QLYY256 pKa = 10.61 KK257 pKa = 10.85 FIGYY261 pKa = 9.98 VSDD264 pKa = 3.62 AFDD267 pKa = 3.42 RR268 pKa = 11.84 RR269 pKa = 11.84 ITNKK273 pKa = 9.82 VYY275 pKa = 10.47 IDD277 pKa = 3.47
Molecular weight: 32.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.268
IPC2_protein 8.302
IPC_protein 8.214
Toseland 8.521
ProMoST 8.624
Dawson 8.975
Bjellqvist 8.975
Wikipedia 9.107
Rodwell 9.048
Grimsley 8.843
Solomon 9.077
Lehninger 9.033
Nozaki 8.99
DTASelect 8.843
Thurlkill 8.887
EMBOSS 9.107
Sillero 9.107
Patrickios 4.151
IPC_peptide 9.063
IPC2_peptide 7.746
IPC2.peptide.svr19 7.707
Protein with the highest isoelectric point:
>tr|K0A152|K0A152_9CIRC Putative capsid protein OS=Dragonfly associated cyclovirus 5 OX=1234883 PE=4 SV=1
MM1 pKa = 7.48 ARR3 pKa = 11.84 FKK5 pKa = 10.86 RR6 pKa = 11.84 VFRR9 pKa = 11.84 RR10 pKa = 11.84 PRR12 pKa = 11.84 RR13 pKa = 11.84 VVRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 MFRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 YY26 pKa = 8.9 RR27 pKa = 11.84 RR28 pKa = 11.84 TRR30 pKa = 11.84 KK31 pKa = 8.47 RR32 pKa = 11.84 TSGTMWCKK40 pKa = 8.19 LTKK43 pKa = 10.37 VATVQVKK50 pKa = 10.38 NDD52 pKa = 3.56 STSVWSGSFYY62 pKa = 10.7 PNDD65 pKa = 3.24 FPEE68 pKa = 4.52 YY69 pKa = 10.63 VSLAPNFEE77 pKa = 5.07 AIQFVKK83 pKa = 9.95 MRR85 pKa = 11.84 MRR87 pKa = 11.84 VMPLQNVSNNSTSQTPAYY105 pKa = 10.68 AMLPWHH111 pKa = 6.81 KK112 pKa = 10.58 GGPLQKK118 pKa = 10.72 AFNTYY123 pKa = 10.81 LSVDD127 pKa = 2.62 KK128 pKa = 11.07 CKK130 pKa = 10.2 IYY132 pKa = 10.72 RR133 pKa = 11.84 QTQQGYY139 pKa = 7.67 QSYY142 pKa = 9.7 KK143 pKa = 10.64 CSVLQNAFTKK153 pKa = 10.34 SSSAISEE160 pKa = 4.34 DD161 pKa = 3.58 FSLATVTKK169 pKa = 7.79 WCPRR173 pKa = 11.84 IEE175 pKa = 4.35 RR176 pKa = 11.84 PNNMEE181 pKa = 3.96 AKK183 pKa = 9.88 QPQIYY188 pKa = 8.89 TGIVVFQGEE197 pKa = 4.14 NTMAGRR203 pKa = 11.84 TTAFNIIQDD212 pKa = 3.59 VWVKK216 pKa = 10.46 CINQSNFSII225 pKa = 4.27
Molecular weight: 26.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.33
IPC2_protein 9.97
IPC_protein 10.818
Toseland 10.877
ProMoST 10.613
Dawson 10.965
Bjellqvist 10.701
Wikipedia 11.199
Rodwell 11.199
Grimsley 11.008
Solomon 11.096
Lehninger 11.067
Nozaki 10.862
DTASelect 10.687
Thurlkill 10.877
EMBOSS 11.286
Sillero 10.891
Patrickios 10.906
IPC_peptide 11.111
IPC2_peptide 9.809
IPC2.peptide.svr19 8.29
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
502
225
277
251.0
29.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.382 ± 0.884
1.992 ± 0.146
4.183 ± 1.242
4.582 ± 1.213
5.378 ± 0.253
6.175 ± 1.659
1.195 ± 0.476
6.574 ± 1.349
7.371 ± 0.446
4.382 ± 0.524
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.988 ± 0.641
5.378 ± 0.534
3.984 ± 0.573
5.378 ± 0.816
8.167 ± 1.302
5.976 ± 1.845
7.371 ± 0.117
6.574 ± 0.903
2.191 ± 0.02
5.777 ± 1.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here